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Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow 
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Sandeep K. Sharma MVSc Ambika P. Singh MVSc PhD Rishi Tayal MVSc Inder S. Chandna MVSc PhD 《Veterinary radiology & ultrasound》1984,25(1):17-22
Contrast radiography of the alimentary canal was conducted in six adult sheep with barium sulfate (70% W/V, 25–30 ml/kg). A period of 12 hours was adequate for opacification of most parts of the gastrointestinal tract. The presence of contrast medium in different parts of the alimentary canal was noted for as long as 60 hours. The right lateral view was adequate for visualization of most structures, but the typical laminar mucosal pattern of the omasum and the spiral mucosal folds of the abomasum were demonstrated better on the ventrodorsal view. 相似文献
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Richa Sood Rekha Khandia Sandeep Bhatia Divakar Hemadri Manoj Kumar Sharan S. Patil Atul K. Pateriya Arshi Siddiqui Malkanna Sanjeev Kumar Mudalagiri Dasappa Venkatesha Diwakar D. Kulkarni 《Tropical animal health and production》2014,46(6):1037-1043
Malignant catarrhal fever (MCF) is a fatal herpesvirus infection of domestic and wild ruminants, with a short and dramatic clinical course characterized primarily by high fever, severe depression, swollen lymph nodes, salivation, diarrhea, dermatitis, neurological disorders, and ocular lesions often leading to blindness. In the present study, fatal clinical cases of sheep associated malignant catarrhal fever (SA-MCF) were identified in cattle in the state of Karnataka. These cases were initially presented with symptoms of diarrhea, respiratory distress, conjunctivitis, and nasal discharges. Laboratory diagnosis confirmed the detection of ovine herpesvirus-2 (OvHV-2) genome in the peripheral blood samples of two ailing animals. The blood samples collected subsequently from sheep of the neighboring areas also showed presence of OvHV-2 genome indicating a nidus of infection in the region. The positive test results were further confirmed by nucleotide sequencing of the OIE approved portion of tegument gene as well as complete ORF8 region of the OvHV-2 genome. Phylogenetic analysis based on the sequence of the latter region indicated close genetic relationship with other OvHV-2 reported elsewhere in the world. 相似文献
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M. N. Shrivastava Arvind Kumar Sandeep Bhandarkar B. C. Shukla K. C. Agrawal 《Euphytica》2003,130(1):143-145
The Asian rice gall midge, Orseolia oryzae Wood Mason (Diptera: Cecidomyiidae), is a major pest of rice in several South and South East Asian countries. The maggots
feed internally on the growing tips of the tillers and transform them into tubular galls, onion leaf-like structures called
‘silver shoots’ resulting into severe yield loss to the rice crop. We studied the mode of inheritance and allelic relationships
of the resistance genes involved in resistant donor Line 9, a sib of a susceptible cultivar ‘Madhuri’. The segregation behaviour
of F1, F2 and F3 populations of the cross between Line 9 and susceptible cultivar MW10 confirmed the presence of a single dominant gene for
resistance. Tests of allelism with all the known genes giving resistance to this population indicated that Line 9 possessed
a new gene which was designated Gm 9
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Sandeep?KumarEmail author Ranjith?P.?Udawatta Stephen?H.?Anderson Ashish?Mudgal 《Agroforestry Systems》2011,83(1):51-62
Buffers have been found to reduce non-point source pollution (NPSP) from watersheds. Hydrologic simulation models assist in
predicting the effects of buffers on runoff and sediment losses from small watersheds. The objective of this study was to
calibrate, validate and simulate runoff and sediment losses and compare buffer effects on NPSP losses relative to control
watersheds (no buffer) for seven years. The experimental design consists of four watersheds under pasture management which
were monitored from 2002 through 2008; two with agroforestry buffers (AgB 100 and AgB 300) and two control watersheds (CW
400 and CW 600). Pasture areas included red clover (Trifolium pretense L.) and lespedeza (Kummerowia stipulacea Maxim.) planted into fescue (Festuca arundinacea Schreb.) while the agroforestry buffer area included Eastern cottonwood trees (Populus deltoids Bortr. ex Marsh.) planted into fescue. The APEX model was calibrated from 2002 to 2005 and was validated from 2006 to 2008.
The r
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and NSE values for the calibration and validation periods of the runoff varied from 0.52 to 0.78 and 0.50 to 0.74, respectively.
The model did not predict sediment loss very well probably due to insufficient number of measured events and low measured
sediment loss. The measured runoff was 57% higher for CW watersheds compared to AgB watersheds. The measured sediment loss
was 95% higher for CW watersheds compared to AgB watersheds. After calibrating and validating the model, it was run for long-term
scenario analyses for 10 years from 1999 to 2008. Simulated buffer width had a significant influence on runoff. Simulated
runoff decreased by 24% when the buffer width was doubled compared to losses associated with the measured buffer width. Simulated
runoff from the CW watersheds was 11% higher with double stocking density (relative to measured density) compared to AgB watersheds
with double stocking density. With half stocking density (relative to measured density), the AgB watershed had 18% lower runoff
compared to CW. Results from this study imply that establishment of agroforestry buffers on grazed pasture watersheds reduce
runoff and sediment losses compared to control watersheds without buffers. 相似文献
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Vinod Kumar Yadav Sandeep Kumar Ravindra K. Panwar 《Genetic Resources and Crop Evolution》2007,54(6):1285-1289
The present investigation was carried out on fifteen germplasm lines of Pisum sativum L. were used for characterization using Randomly Amplified Polymorphic DNA (RAPD) markers. While 12 random primers were taken,
out of them 11 primers gave amplification. These primers gave a total of 133 bands out of which 106 were polymorphic. Genetic
similarities of the RAPD profiles were estimated by using Jaccard’s coefficient with NTSYSpc 2.0 software. The similarity
index values ranged from 0.263 to 0.793 indicating the presence of enormous genetic diversity at molecular level. A dendrogram
generated by cluster analysis divided fifteen fieldpea genotypes into two Groups A and B. Major Group A have five genotypes
and major Group B have nine genotypes. 相似文献
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Kurkure NV Pande VV Thomas F Bhandarkar AG 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2006,53(1):22-23
A case of seminoma in a monorchid adult guinea fowl (Numida meleagris) is described. Grossly, a right enlarged testis, which was soft in consistency, and white to pale in colour with few spots of haemorrhages was observed. Histologically, the testicle revealed diffusely spread sheets of tumour cells. The cells were large pleomorphic with eccentrically placed hyperchromic nuclei. Mitotic figures were evident. A scanty fibrous stroma, containing lymphocytes and histiocytes, separating the groups of tumour cells, along with few areas of haemorrhages were observed. Occurrence of seminoma in guinea fowl is unusual and hence reported. 相似文献
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Subhash Verma Mandeep Sharma Shailja Katoch Lovit Verma Sandeep Kumar Vishal Dogra Rajesh Chahota Prasenjit Dhar Geetanjali Singh 《Veterinary research communications》2013,37(1):83-89
Pasteurella multocida is a causative agent of many major diseases of which haemorrhagic septiciemia (HS) in cattle & a buffalo is responsible for significant losses to livestock sector in India and south Asia. The disease outcome is affected by various host- and pathogen-specific determinants. Several bacterial species-specific putative virulence factors including the capsular and virulence associated genes have been proposed to play a key role in this interaction. A total of 23 isolates of P. multocida were obtained from 335 cases of various clinically healthy and diseased cattle. These isolates were examined for capsule synthesis genes (capA, B, D, E and F) and eleven virulence associated genes (tbpA, pfhA, toxA, hgbB, hgbA, nanH, nanB, sodA, sodC, oma87 and ptfA) by PCR. A total of 19 P. multocida isolates belonging to capsular type B and 4 of capsular type A were isolated. All isolates of capsular type B harboured the virulence associated genes: tbpA, pfhA, hgbA, sodC and nanH, coding for transferrin binding protein, filamentous hemagglutinin, haemoglobin binding protein, superoxide dismutase and neuraminidases, respectively; while isolates belonging to capsular type A also carried tbpA, pfhA, hgbA and nanH genes. Only 50 % of capsular type A isolates contained sodC gene while 100 % of capsular type B isolates had sodC gene. The gene nanB and toxA were absent in all the 23 isolates. In capsular type A isolates, either sodA or sodC gene was present & these genes did not occur concurrently. The presence of virulence associated gene ptfA revealed a positive association with the disease outcome in cattle and could therefore be an important epidemiological marker gene for characterizing P. multocida isolates. 相似文献