Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow          下载免费PDF全文

Pradeep Jatav  Monika Sodhi  Ankita Sharma  Sandeep Mann  Amit Kishore  Umesh K Shandilya  Ashok K Mohanty  Ranjit S Kataria  Poonam Yadav  Preeti Verma  Surinder Kumar  Dhruba Malakar  Manishi Mukesh 《Animal Science Journal》2016,87(3):344-353

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Secretion of dopamine and norepinephrine in hypophyseal portal blood and prolactin in peripheral blood of Holstein cattle     

Hard DL  Bhatnagar RK  Molina JR  Anderson LL 《Domestic animal endocrinology》2001,20(2):89-100

The objective was to test the hypothesis that dopamine regulates prolactin (PRL) secretion by determining acute changes in catecholamine concentrations in hypophyseal portal blood of cattle, and their relation to peripheral blood concentration of PRL in hypophyseal stalk-transected (HST) and sham-operated controls (SOC). Holstein heifers (606 +/- 21 kg BW; mean +/- SE) were subjected to neurosurgery for 8 h to collect hypophyseal portal blood with a stainless steel cannula designed with a cuff placed under the pituitary stalk and peripheral blood via a jugular vein catheter. PRL plasma concentration was measured by radioimmunoassay, and dopamine and norepinephrine in portal plasma by radioenzymatic assay. During anesthesia before HST or SOC, PRL plasma concentration ranged from 20-40 ng/ml throughout 255 min. PRL abruptly increased and remained above 90 ng/ml after HST compared with a steady decrease to <20 ng/ml in SOC heifers throughout 440 min. Within 5 min after severing the hypophyseal stalk, dopamine in portal blood (>8 ng/ml) was significantly increased (P < 0.05) compared with peripheral blood (<2 ng/ml). Norepinephrine concentration in portal blood was significantly greater (P < 0.05) than in peripheral blood during the first 60 min. The sustained high PRL level in peripheral plasma after severing the hypophyseal stalk stimulated hypothalamic dopamine secretion from hypophyseal portal vessels during the prolonged period of blood collection. Norepinephrine concentration in these cattle was greater in hypophyseal portal than in peripheral blood, implicating both an important hypothalamic source of the catecholamine as well as an adrenal gland contribution during anesthesia.  相似文献   

A preliminary report of the isolation of a lentogenic strain of Newcastle disease virus (CDF 66) from a pig     

B S Malik  A Bhatnagar  N D Verma 《The British veterinary journal》1969,125(8):Suppl:26-Suppl:28

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CONTRAST RADIOGRAPHY OF THE OVINE GASTROINTESTINAL TRACT     

Sandeep K.  Sharma  MVSc  Ambika P.  Singh  MVSc  PhD  Rishi  Tayal  MVSc  Inder S.  Chandna  MVSc  PhD 《Veterinary radiology & ultrasound》1984,25(1):17-22

Contrast radiography of the alimentary canal was conducted in six adult sheep with barium sulfate (70% W/V, 25–30 ml/kg). A period of 12 hours was adequate for opacification of most parts of the gastrointestinal tract. The presence of contrast medium in different parts of the alimentary canal was noted for as long as 60 hours. The right lateral view was adequate for visualization of most structures, but the typical laminar mucosal pattern of the omasum and the spiral mucosal folds of the abomasum were demonstrated better on the ventrodorsal view.  相似文献   

Path coefficient analysis of correlation between breeding cycles of the common myna Acridotheres tristis (Passeriformes: Sturnidae) and its phthirapteran ectoparasites     

Srivastava R  Kumar S  Gupta N  Singh SK  Saxena AK 《Folia parasitologica》2003,50(4):315-316

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Moisture-dependent physical properties of jatropha fruit     

R.C. Pradhan  S.N. Naik  N. Bhatnagar  V.K. Vijay 《Industrial Crops and Products》2009,29(2-3):341-347

The physical properties of fruit are important in designing and fabricating equipment and structures for handling, transporting, processing and storage, and also for assessing quality. The study was conducted to investigate some physical properties of jatropha fruit at various moisture levels. The average length, width, thickness and 1000 mass were 29.31 mm, 22.18 mm, 21.36 mm and 1522.10 g, respectively, at moisture content of 7.97% d.b. The geometric mean diameter increased from 24.03 to 24.70 mm and the sphericity varied between 0.82 and 0.83 as moisture content increased from 7.97% to 23.33% d.b., respectively. In the same moisture range, the bulk and true densities decreased from 278 to 253 and 546 to 435 kg m^?3, respectively, whereas the corresponding porosity also decreased from 49.08% to 41.84%. As the moisture content increased from 7.97% to 23.33% d.b., crushing strength was decreased from 275 to 79 N, whereas the angle of repose and surface areas were found to increase from 36.41° to 41.72° and 1815.73 to 1917.59 mm², respectively. The static coefficient of friction of jatropha fruit increased linearly against the surfaces of three structural materials, namely plywood (47.81%), mild steel (62.88%) and aluminium (34.82%) as the moisture content increased from 7.97% to 23.33% d.b.  相似文献   

Cartilage lesions associated with shaky-leg lameness in turkeys     

R J Julian  M K Bhatnagar 《Avian diseases》1985,29(1):218-232

Shaky-leg lameness is a poorly defined, mild to very severe, painful lameness occurring mainly in 10-to-18-week-old fast-growing male turkeys. Since there are no significant gross or microscopic lesions in early or uncomplicated cases, the cause of the lameness is likely soft-tissue (muscle or tendon) pain. A case history of a flock problem of shaky-leg lameness is described, and it is suggested that stiffness and inactivity secondary to footpad dermatitis in these turkeys was responsible for the severe shaky-leg lameness in the flock. The cartilage lesion described is an avascular lesion and appeared to be the result of inactivity rather than the cause of lameness in these turkeys.  相似文献   

Moisture-dependent physical properties of jatropha seed (Jatropha curcas L.)     

D.K. Garnayak  R.C. Pradhan  S.N. Naik  N. Bhatnagar 《Industrial Crops and Products》2008,27(1):123-129

The study was conducted to investigate some moisture-dependent physical properties of jatropha seed namely, seed dimension, 1000 seed mass, surface area, sphericity, bulk density, true density, angle of repose and static coefficient of friction against different materials. The physical properties of jatropha seed were evaluated as a function of moisture content in the range of 4.75–19.57% d.w. The average length, width, thickness and 1000 seed mass were 18.65 mm, 11.34 mm, 8.91 mm and 741.1 g, respectively at moisture content of 4.75% d.w. The geometric mean diameter and sphericity increased from 12.32 to 12.89 mm and 0.66 to 0.67 as moisture content increased from 4.75 to 19.57% d.w., respectively. In the same moisture range, densities of the rewetted jatropha seed decreased from 492 to 419 kg m⁻³, true density increased from 679 to 767 kg m⁻³, and the corresponding porosity increased from 27.54 to 45.37%. As the moisture content increased from 4.75 to 19.57% d.w., the angle of repose and surface area were found to increase from 28.15° to 39.95° and 476.78 to 521.99 mm², respectively. The static coefficient of friction of jatropha seed increased linearly against the surfaces of three structural materials, namely plywood (44.12%), mild steel sheet (64.15%) and aluminum (68.63%) as the moisture content increased from 4.75 to 19.57% d.w.  相似文献   

Detection and molecular characterization of naturally transmitted sheep associated malignant catarrhal fever in cattle in India     

Richa Sood  Rekha Khandia  Sandeep Bhatia  Divakar Hemadri  Manoj Kumar  Sharan S. Patil  Atul K. Pateriya  Arshi Siddiqui  Malkanna Sanjeev Kumar  Mudalagiri Dasappa Venkatesha  Diwakar D. Kulkarni 《Tropical animal health and production》2014,46(6):1037-1043

Malignant catarrhal fever (MCF) is a fatal herpesvirus infection of domestic and wild ruminants, with a short and dramatic clinical course characterized primarily by high fever, severe depression, swollen lymph nodes, salivation, diarrhea, dermatitis, neurological disorders, and ocular lesions often leading to blindness. In the present study, fatal clinical cases of sheep associated malignant catarrhal fever (SA-MCF) were identified in cattle in the state of Karnataka. These cases were initially presented with symptoms of diarrhea, respiratory distress, conjunctivitis, and nasal discharges. Laboratory diagnosis confirmed the detection of ovine herpesvirus-2 (OvHV-2) genome in the peripheral blood samples of two ailing animals. The blood samples collected subsequently from sheep of the neighboring areas also showed presence of OvHV-2 genome indicating a nidus of infection in the region. The positive test results were further confirmed by nucleotide sequencing of the OIE approved portion of tegument gene as well as complete ORF8 region of the OvHV-2 genome. Phylogenetic analysis based on the sequence of the latter region indicated close genetic relationship with other OvHV-2 reported elsewhere in the world.  相似文献   

A new gene for resistance in rice to Asian rice gall midge (Orseolia oryzae Wood Mason) biotype 1 population at Raipur, India     

M. N. Shrivastava  Arvind Kumar  Sandeep Bhandarkar  B. C. Shukla  K. C. Agrawal 《Euphytica》2003,130(1):143-145

The Asian rice gall midge, Orseolia oryzae Wood Mason (Diptera: Cecidomyiidae), is a major pest of rice in several South and South East Asian countries. The maggots feed internally on the growing tips of the tillers and transform them into tubular galls, onion leaf-like structures called ‘silver shoots’ resulting into severe yield loss to the rice crop. We studied the mode of inheritance and allelic relationships of the resistance genes involved in resistant donor Line 9, a sib of a susceptible cultivar ‘Madhuri’. The segregation behaviour of F₁, F₂ and F₃ populations of the cross between Line 9 and susceptible cultivar MW10 confirmed the presence of a single dominant gene for resistance. Tests of allelism with all the known genes giving resistance to this population indicated that Line 9 possessed a new gene which was designated Gm 9 This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献