Identification of bovine serum albumin as an IgE-reactive beef component in a dog with food hypersensitivity against beef     

Ohmori K  Masuda K  Kawarai S  Yasuda N  Sakaguchi M  Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(8):865-867

IgE-reactive beef components were examined by an immunoblot analysis using a serum from a dog with food hypersensitivity against beef. The immunoblot analysis revealed a distinct band at approximately 66 kDa and a faint band at approximately 50 kDa. The immunoblot analysis for serum IgE reactivity to bovine serum albumin (BSA) also revealed a positive band at 66 kDa. Serum IgE reactivity to the 66-kDa protein of beef was diminished by pre-incubating the serum sample with BSA. Furthermore, a positive reaction to BSA was detected in intradermal testing in the dog. These results clearly indicated that BSA was an IgE-reactive beef component in the dog with food hypersensitivity against beef.  相似文献   

Afferent and efferent connections of the nucleus rotundus demonstrated by WGA-HRP in the chick   总被引：5，自引：0，他引：5  

Hu M  Naito J  Chen Y  Ohmori Y  Fukuta K 《Anatomia, histologia, embryologia》2003,32(6):335-340

Organization of the fibre connections in the chick nucleus rotundus (Rt) was investigated by an axonal tracing method using wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP). After an injection of WGA-HRP into the Rt, labelled neurones were observed in the striatum griseum centrale (SGC) in both sides of the tectum (TO) and in the ipsilateral nucleus subpretectalis/nucleus interstito-pretecto-subpretectalis (SP/IPS). Labelled fibres and terminals were also found in the ipsilateral ectostriatum (Ect). These fibre connections were topographically organized rostrocaudally. In the TO-Rt projection, the rostral and the dorsocaudal parts of the Rt received afferents from the superficial part of the SGC, the middle part of the Rt received afferents from the intermediate part of the SGC, and the ventrocaudal part of the Rt received mainly fibres from the deep part of the SGC. These topographic projections were accompanied by a considerable number of diffuse projections to the thalamic regions surrounding the Rt. In addition, the rostral and middle caudal parts of the Rt received afferents from the lateral and medial parts of the SP/IPS, respectively, and respective parts of the Rt sent efferents to the lateral and medial parts of the Ect.  相似文献   

Histo-and Cytochemical Studies on the Distribution of Acetylcholinesterase-positive Nerve Fibers in the Goat Adrenal Gland     

Tohru  Watanabe  Kohzy  Hiramatsu  Yasushige  Ohmori Young-Ki  Paik 《Anatomia, histologia, embryologia》1990,19(3):245-254

The distribution patterns of acetylcholine esterase positive nerve fibers in the goat adrenal gland is described. Some positive cells are observed within the capsule. Terminals were identified in both the medulla and the cortex of the gland.  相似文献   

Muscle Representation within the Hypoglossal Nucleus of the Chicken Studied by Means of Horseradish Peroxidase     

J. Wang  T. Watanabe  M. Suzuki  Y. Ohmori  J. Naito 《Anatomia, histologia, embryologia》1996,25(4):277-282

The distribution in the chicken of motoneurons innervating the hyolingual muscles, i.e. the Mm. hyoglossus rostralis (HR), hyoglossus obliquus (HO), ceratoglossus (CG), interceratobranchialis (CB). stylohyoideus (YH), serpihyoideus (PH) and cricohyoideus (CR), and the laryngotracheal muscles, comprising the Mm. tracheolateralis (TL), cleido-hyoideus (CL) and sternotrachealis (ST), was examined by retrograde transport of horseradish peroxidase conjugated with wheat-germ agglutinin. Labelled motoneurons are only found in the hypoglossal nucleus. The rostrocaudal distributions of motoneurons projecting to hyolingual muscles are restricted in the hypoglossal nucleus cranial to the obex, and those projecting to laryngotracheal muscles are distributed in the more caudal part of hypoglossal nucleus. Detailed analysis of the data showed that the most rostrally positioned motoneurons in the hypoglossal nucleus supplied to the PH, followed by the CG, CB. HR, YH, HO, CR, TL, CL and ST in that order, overlapping each other. In the hypoglossal nucleus motoneurons innervating the PH and YH have the smallest perikarya. Of the motoneurons in the hypoglossal nucleus, those supplying the laryngotracheal muscles (CL and TL) have the largest perikarya. Motoneurons innervating the other muscles are intermediate in size.  相似文献   

Development of respiratory enzyme activities during spore germination   总被引：2，自引：0，他引：2  

K Ohmori  D Gottlieb 《Phytopathology》1965,55(12):1328-1336

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Localization of the motoneurons innervating the hindlimb muscles in the spinal cord of the domestic fowl     

Y Ohmori  T Watanabe  T Fujioka 《Anatomia, histologia, embryologia》1984,13(2):141-155

The origins of the motor nerve fibers supplying the individual hindlimb muscles were elucidated in the fowl by the retrograde degeneration method. Chromatolytic cells were seen in the ipsilateral lamina 9 of the lumbosacral enlargement. Motoneurons innervating the individual hindlimb muscles were localized in the characteristic position of lamina 9. Both hip and thigh muscles are supplied by motoneurons of almost all the columns in lumbar segments and by those of dorsolateral columns in sacral segments, while the shank and foot muscles are supplied by motoneurons of ventromedial columns in sacral segments. The origins of the dorsal cord of the lumbosacral plexus are situated laterally in the lamina 9 and those of the ventral cord are located medially in it.  相似文献   

Glucose uptake activity in murine red blood cells infected with Babesia microti and Babesia rodhaini     

Ohmori T  Adachi K  Fukuda Y  Tamahara S  Matsuki N  Ono K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(8):945-949

The glucose uptake activity in Babesia rodhaini and B. microti - infected red blood cell (IRBC) was investigated in mice using 2-deoxy-D-glucose (2DOG) and L-glucose (L-Glc), a non-metabolizable analogue of D-glucose and non-incorporative glucose to non-infected RBC (NRBC), respectively. The uptake activities of both DOG and L-Glc were higher in IRBCs than those in NRBC. The concentration dependent uptake of 2DOG and L-Glc in both IRBC revealed a linear curve, indicating non-transporter mediated uptake. In addition, B. microti IRBC showed higher 2DOG uptake than B. rodhaini IRBC, whereas no difference was observed in L-Glc uptake. These results indicated that some new glucose uptake system, at least two systems, developed in both IRBC. The new systems were sodium independent, non-competitive to L-Glc, and sensitive to temperature. One of two systems had no kinetical difference between B. rodhaini and B. microti IRBC, however another one might have higher uptake activity in B. microti IRBC compared to that in B. rodhaini IRBC.  相似文献   

Cardiovascular effects of a phosphodiesterase III inhibitor in the presence of carvedilol in dogs     

Uechi M  Hori Y  Fujimoto K  Ebisawa T  Yamano S  Maekawa S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(6):549-553

The aim of this study was to determine whether dobutamine, dopamine, or milrinone (a phosphodiesterase [PDE] III inhibitor) would support cardiac function that had been attenuated by administration of the beta-blocker, carvedilol (0.2, 0.4, or 0.8 mg/kg). Hemodynamic and cardiac parameters including the heart rate (HR), left-ventricular fractional shortening (FS), and arterial pressure were measured in six healthy dogs without cardiac disease. Carvedilol did not affect FS or arterial pressure, but decreased the HR significantly. The positive inotropic and chronotropic responses to dobutamine and dopamine were attenuated by carvedilol, whereas arterial pressure was unaffected. Milrinone did not affect the HR and decreased arterial pressure, whereas FS was significantly greater both in the control and carvedilol-treated groups. Although milrinone affect the negative chronotropic effects of carvedilol, milrinone increased FS and prevented the decrease in arterial pressure. These results suggest that inhibition of PDE III preserves cardiac contractility and hemodynamic function in the presence of carvedilol.  相似文献   

Identification of c-kit mutations-independent neoplastic cell proliferation of canine mast cells     

Ohmori K  Kawarai S  Yasuda N  Tanaka A  Matsuda H  Nishimura R  Sasaki N  Tsujimoto H  Masuda K 《Veterinary immunology and immunopathology》2008,126(1-2):43-53

Gain-of-function mutations in the proto-oncogene c-kit have been considered the molecular mechanism of neoplastic proliferation of mast cells. However, the importance of c-kit gene mutations is not well evaluated in canine mast cell tumors (MCTs). In the present study, we established and characterized a mast cell line, HRMC, derived from a dog with MCT. We also examined c-kit mutations in HRMC cells and assessed an inhibitory effect of a tyrosine kinase inhibitor, STI571, on HRMC cells. HRMC cells had cytoplasmic metachromatic granules, chymase and tryptase, and expressed both KIT and FcepsilonRI on the cell surface. HRMC cells contained histamine and released beta-hexosaminidase through FcepsilonRI cross-linking and calcium ionophore stimulation. Nucleotide sequence analysis demonstrated no mutations in an open reading frame of c-kit cDNA and genomic DNA of the juxtamembrane domain of c-kit in HRMC cells. STI571 did not show any inhibitory effects on the proliferation of HRMC cells. These findings clearly demonstrated the existence of c-kit mutations-independent neoplastic canine mast cell proliferation. The growth factor-independent mast cell line established in this study might be valuable to explore novel mechanisms of c-kit mutations-independent neoplastic proliferation of mast cells in dogs.  相似文献   

Pilot evaluation of the efficacy of shampoo treatment with ultrapure soft water for canine pruritus     

Keitaro Ohmori  Akane Tanaka  Yuka Makita  Masaki Takai  Yuji Yoshinari  Hiroshi Matsuda 《Veterinary dermatology》2010,21(5):477-483

Ultrapure soft water (UPSW) is water in which calcium and magnesium ions have been replaced with sodium ions using a cation‐exchange resin. We recently demonstrated that washing with soap and UPSW reduced the clinical severity of dermatitis and improved the skin barrier function in NC/NgaTnd mice, a murine model for human atopic dermatitis. The purpose of this pilot study was to evaluate the efficacy of shampoo treatment with UPSW for dogs with pruritus. Eleven dogs with pruritus were randomly assigned to two groups depending on whether they received weekly shampoo treatment with UPSW or tap water for 4 weeks. After a washout period, the treatment protocol was switched such that each dog received both treatments. The pre‐treatment and post‐treatment values of the following were compared: pruritus scores assessed by the owners; dermatitis scores recorded by an investigator; and transepidermal water loss (TEWL). Shampoo treatment with UPSW significantly decreased pruritus and dermatitis scores in the dogs, whereas shampoo treatment with tap water did not. In addition, shampoo treatment with UPSW, but not with tap water, significantly reduced TEWL in the dogs. Adverse events due to the treatment were not observed in the dogs. Furthermore, we found that topical application of UPSW for barrier‐disrupted skin caused by tape stripping in healthy dogs decreased TEWL more rapidly than topical application of tap water. Our findings suggest that shampoo treatment with UPSW promotes skin barrier recovery and thus could be considered as a possible therapeutic option in the management of pruritus and dermatitis in dogs.  相似文献