Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers          下载免费PDF全文

Kapil K. Tiwari  Anshuman Singh  Sasmita Pattnaik  Maninder Sandhu  Sukhdeep Kaur  Sourabh Jain  Sushma Tiwari  Shweta Mehrotra  Mahender Anumalla  Rashmita Samal  Jyoti Bhardwaj  Neha Dubey  Vikrant Sahu  Gayle A. Kharshing  Patu K. Zeliang  Kadiri Sreenivasan  Pankaj Kumar  Swarup K. Parida  Sevanthi V. A. Mithra  Vandana Rai  Wricha Tyagi  Pawan K. Agrawal  Atmakuri R. Rao  Arunava Pattanayak  Girish Chandel  Ashok K. Singh  Ishwari S. Bisht  Kangila V. Bhat  Gundimeda J. N. Rao  Jitendra P. Khurana  Nagendra K. Singh  Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171

Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining.  相似文献   

Antiarthritic gold compounds effectively quench electronically excited singlet oxygen   总被引：5，自引：0，他引：5  

E J Corey  M M Mehrotra  A U Khan 《Science (New York, N.Y.)》1987,236(4797):68-69

Although certain gold [Au(I)] compounds have been used effectively in the treatment of rheumatoid arthritis for some years, the molecular basis for such therapeutic action has been unclear. One possible mechanism of the action of Au(I) compounds is that they protect unsaturated membrane lipids and proteins against oxidative degradation caused by activated phagocytes that are not properly regulated. In this study it has been shown that superoxide ion (O-2.), a product of activated phagocytes, can be oxidized to electronically excited singlet oxygen (O1(2)delta g), an agent that is capable of peroxidation of unsaturated fatty acid derivatives. It has also been shown that antiarthritic Au(I) compounds are effective deactivators of O1(2)delta g with quenching constants on the order of 10(7) M-1 sec-1.  相似文献   

Studies on the association of Aspergillus fumigatus with ocular infections in animals     

M Pal  B S Mehrotra 《The Veterinary record》1986,118(2):42-44

The occurrence and aetiological significance of Aspergillus fumigatus, an opportunistic pathogen, have been studied in 93 animals with various ophthalmological problems. A total of 93 eye swabs collected from 35 mules, 26 dogs, 13 fowl, 11 cattle, five buffaloes and three camels were investigated mycologically for the presence of A fumigatus. The pathogen was isolated in pure and heavy growth from the swabs from two dogs, one bull, one mule and one fowl. The fungus was also demonstrated directly in clinical material by the potassium hydroxide technique. A fumigatus could not be cultured from the buffaloes and camels. All the five cases had been treated with broad spectrum antibiotics and cortisone and two had received traumatic injury to the eyes (one mule and a bull). The organism was not isolated in pure culture from the conjunctival swabs of 22 apparently healthy animals (11 dogs, six mules, three fowl and two cattle). Many other saprophytic fungi were recovered in mixed cultures but were considered to be contaminants. The clinical signs and diagnostic criteria of oculomycosis have been discussed.  相似文献   

Inheritance and allelic relationship among gene(s) for blast resistance in pearl millet [Pennisetum glaucum (L.) R. Br.]          下载免费PDF全文

Shweta Singh  Rajan Sharma  Bheemavarapu Pushpavathi  Shashi Kumar Gupta  Ch. V. Durgarani  Chandramani Raj 《Plant Breeding》2018,137(4):573-584

Six blast‐resistant pearl millet genotypes, ICMB 93333, ICMB 97222, ICMR 06444, ICMR 06222, ICMR 11003 and IP 21187‐P1, were crossed with two susceptible genotypes, ICMB 95444 and ICMB 89111 to generate F₁s, F₂s and backcrosses, BC₁P₁ (susceptible parent × F₁) and BC₁P₂ (resistant parent × F₁) for inheritance study. The resistant genotypes were crossed among themselves in half diallel to generate F₁s and F₂s for test of allelism. The F₁, F₂ and backcross generations, and their parents were screened in a glasshouse against Magnaporthe grisea isolates Pg 45 and Pg 53. The reaction of the F₁s, segregation pattern of F₂s and BC₁P₁ derived from crosses involving two susceptible parents and six resistant parents revealed the presence of single dominant gene governing resistance in the resistant genotypes. No segregation for blast reaction was observed in the F₂s derived from the crosses of resistant × resistant parents. The resistance reaction of these F₂s indicated that single dominant gene conferring resistance in the six genotypes is allelic, that is same gene imparts blast resistance in these genotypes to M. grisea isolates.  相似文献   

Replacement of de‐oiled rice bran by soaked and fermented sweet potato leaf meal: Effect on growth performance,body composition and expression of insulin‐like growth factor 1 in Labeo rohita (Hamilton), fingerlings          下载免费PDF全文

Shweta Meshram  Ashutosh D Deo  Sarvendra Kumar  Md Aklakur  Narottam P Sahu 《Aquaculture Research》2018,49(8):2741-2750

A 60‐day feeding trial was conducted to determine the growth performance and expression of insulin‐like growth factor 1 gene (IGF‐I gene) in Labeo rohita fingerlings fed with either raw, soaked or fermented sweet potato leaf meal (SPLM) by completely replacing de‐oiled rice bran (DORB), following a completely randomized design. Seven isonitrogenous (30%) and isocaloric (1.8 MJ/100 g) diets were prepared by replacing DORB with 50% and 100% raw, soaked and fermented sweet potato leaf meal, maintaining DORB‐containing diets as a control. Weight gain %, SGR (specific growth rate) and PER (protein efficiency ratio) were significantly (p < 0.05) higher when 100% DORB was replaced by fermented SPLM in comparison to other treatment groups. The fermented and soaked SPLM‐fed groups had registered with lower FCR value. The expression of growth regulating gene IGF‐I mRNA and RNA/DNA ratio was found to be significantly higher (p < 0.05) in soaked and fermented SPLM‐fed groups. In this study, the body protein and lipid composition did not vary significantly (p > 0.05). Hence, the study concludes that the fermented sweet potato leaf meal using Chaetomium globosum can replace 100% DORB in the diet of Labeo rohita without any detrimental effect on growth performance.  相似文献   

Immune Responses and Protective Efficacy of Binary Ethylenimine (BEI)-Inactivated Bluetongue Virus Vaccines in Sheep   总被引：2，自引：0，他引：2  

Ramakrishnan MA  Pandey AB  Singh KP  Singh R  Nandi S  Mehrotra ML 《Veterinary research communications》2006,30(8):873-880

  相似文献   

Retraction notice to “Fluctuations of certain biochemical constituents and markers enzymes as a consequence of monocrotophos toxicity in the edible freshwater fish, Channa punctatus” [Pesticide Biochemistry and Physiology 94 (2009) 5-9]     

Shweta Agrahari 《Pesticide biochemistry and physiology》2010,96(3):141-146

A total of 185 hexanic, dichloromethanic, ethanolic and hydroethanolic extracts from 24 species of Cerrado plants, were tested against Zabrotes subfasciatus, Acanthoscelides obtectus, and human saliva α-amylases. Twelve crude extracts presented inhibition rates greater than 80% against digestive α-amylases of the insect pest Z. subfasciatus, at a concentration of 1 mg mL⁻¹. These extracts were also tested against A. obtectus and human saliva α-amylases to verify their affinity and specificity of action. The hydroethanolic Kielmeyera coriacea stem bark extract presented a strong inhibitory potential, with IC₅₀ values of 110 μg mL⁻¹ for Z. subfasciatus and 272.12 μg mL⁻¹ for A. obtectus, in addition to a 97.09% reduction in enzyme activity of human saliva α-amylases at 125 μg mL⁻¹. The hexanic Aspidosperma macrocarpon root wood extract totally inhibited the activity of Z. subfasciatus α-amylases, reduced the enzyme activity of A. obtectus by 14.69% at 1 mg mL⁻¹, but did not alter the activity of human saliva α-amylases, thus characterizing greater inhibition affinity and specificity. The results suggest that the application of plant extracts against insect α-amylases represent a promising biotechnological tool for development of new insect pest control strategies, with noticeable affinity and specificity of action against different target enzymes.  相似文献   

Pseudocercospora kaiseri sp. nov. causing a necrotic leaf spot of Erythrina     

M. D. Mehrotra 《Forest Pathology》1991,21(2):124-126

A new species, Pseudocercospora kaiseri, causing leaf spot of Erythrina stricta Roxb. from India is described and illustrated.  相似文献   

Effects of dieldrin and sumithion on the amino acid nitrogen and proteins in the haemolymph of desert locust Schistocerca gregaria Forsk     

A.P. Kulkarni  K.N. Mehrotra 《Pesticide biochemistry and physiology》1973,3(4):420-434

The changes in amino acid nitrogen, proline, and proteins in the haemolymph of desert locusts at different stages of dieldrin and sumithion posioning are reported using techniques of colorimetry, polyacrylamide gel electrophoresis, Sephadex gel chromatography, and electro-gel chromatography.Both the insecticides did not alter amino acid nitrogen and proline titers during the initial stages of poisoning. However, drastic changes were noted at the later stages. In comparison to amino acid nitrogen, the depletion in proline content was of much higher magnitude. Initially the protein content of haemolymph depleted and increased significantly at the acute poisoning stage. Several changes in the electrophoregrams and protein elution profiles were noted in the haemolymph samples of treated insects. Many new proteins were detected in treated insects and their molecular weights, diffusion coefficients, and Stoke's radii are reported.These results are discussed in relation to current ideas of protein metabolism in insects. Further, a possible role of haemolymph proteins as insecticide carriers has been postulated.  相似文献   

Nucleotide sequence and vaccinia expression of the nucleoprotein of a highly virulent, neurotropic strain of Newcastle disease virus   总被引：3，自引：0，他引：3  

Ward MD  Fuller FJ  Mehrotra Y  De Buysscher EV 《Avian diseases》2000,44(1):34-44

The nucleoprotein (NP) of Newcastle disease virus (NDV) was selected to study the relative importance of an internal structural protein in the avian immune response. The NP gene of the virulent, neurotropic NDV Texas GB (TGB) strain was cloned and sequenced. Nucleotide sequence data for the NP gene allowed comparison of the deduced amino acid sequences for the NP genes of NDV-TGB and the avirulent duck isolate NDV-D26. These comparisons demonstrated an 89% nucleotide sequence homology and a 97% homology between the deduced amino acid sequences. The NDV-TGB NP expressed in recombinant vaccinia virus (rVAC) was electrophoretically and immunologically identical to the wild-type NDV-TGB. Although inoculation of chickens with the recombinant vaccinia virus expressing the NDV NP gene elicited anti-NDV antibodies in higher titers than in birds inoculated with live LaSota NDV, this strong anti-NDV response did not protect against lethal challenge with NDV-TGB.  相似文献