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The effects of 3 different methods for removing the initial air on the properties of fermented plant beverages produced from phom-nang seaweed (Gracilaria fisheri) and wild forest noni (Morinda coreia Ham.) were investigated. Only method M which covered the space above the fermentation liquid with a water filled plastic bag produced no surface film of yeast, had the highest acidity and also antibacterial activity from both plants after 90 days of fermentation. However, the yeast count still exceeded the standard guidelines for plant beverages. The fermented beverage from wild forest noni showed more antibacterial activity against 3 of 4 pathogenic bacteria tested than that from the phomnang seaweed, probably for its higher levels of acidity and ethanol content. Lactic Acid Bacteria (LAB) isolated from the fermentation samples from days 1-5 using the method M from both fermented plant beverages were Leuconostoc mesenteroides supsp. mesenteroides and Leu. mesenteroides subsp. dextranicum while presence of Lactobacilus plantarum was only recorded at days 4-5 in the wild forest noni beverage. From days 6-14 the isolates were Lactobacillus plantarum, Lactobacillus fermentum and Lactobacillus brevis from wild forest noni beverage, whereas only L. brevis was not detected in the seaweed beverage. During days 21-45 both beverages had a similar LAB population of L. plantarum and L. brevis while L. coryniformis was only found in the wild forest noni beverage. Between days 60-90 in both plant beverages only L. plantarum and Lactobacillius sp. were detected. 相似文献
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Wilawan Khanitchaidecha Maneesha Shakya Kamei Tatsuru Futaba Kazama 《Water, air, and soil pollution》2012,223(7):3939-3953
To provide good quality of drinking water, a biological system to remove ammonium-nitrogen (NH4-N) from groundwater was studied in this research. The NH4-N removal system consists of two attached growth reactors: one for nitrification and the other for hydrogenotrophic denitrification (H. denitrification). The nitrification reactor, fed by the NH4-N contained water, could remove NH4-N without any need of aeration. The nitrification efficiency was increased by reactor length; the highest efficiency of 92?% was achieved at the longest reactor of 100?cm. A high Fe in groundwater affected the reactor performance by decreasing the efficiency, while a low inorganic carbon (IC) had no effects. Despite of good efficiency in terms of NH4-N removal, the nitrification reactor increased the concentration of NO3-N in its effluent. To treat the NO3-N, a H. denitrification reactor was set up after the nitrification reactor. Efficiency of the H. denitrification reactor was enhanced by increasing H2 flow rates. The efficiencies were 3, 27, and 90?% for 30, 50, and 70?mL/min of H2 flow rates, respectively. It was also found that the NO3-N contained water (water from the nitrification reactor) had to supply IC (i.e., NaHCO3 or CO2) for efficient H. denitrification; however, an on-site reactor showed that it can be achieved even without IC addition. The treated water contained low NH4-N and NO3-N of <1.5 and <11.3?mg/L, respectively, which comply with drinking water standards. The good performance of the reactors in terms of high efficiency, no aeration need, and low H2 supply indicated appropriateness of the system for groundwater treatment. 相似文献
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Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood 总被引：3，自引：0，他引：3
Alhassan A Pumidonming W Okamura M Hirata H Battsetseg B Fujisaki K Yokoyama N Igarashi I 《Veterinary parasitology》2005,129(1-2):43-49
With the aim of developing more simple diagnostic alternatives, a differential single-round and multiplex polymerase chain reaction (PCR) method was designed for the simultaneous detection of Babesia caballi and Babesia equi, by targeting 18S ribosomal RNA genes. The multiplex PCR amplified DNA fragments of 540 and 392 bp from B. caballi and B. equi, respectively, in one reaction. The PCR method evaluated on 39 blood samples collected from domestic horses in Mongolia yielded similar results to those obtained from confirmative PCR methods that had been established earlier. Thus, the single-round and multiplex PCR method offers a simple tool for the differential diagnosis of B. caballi and B. equi infections in routine diagnostic laboratory settings as well as in epidemiological studies. 相似文献
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Asadhawut Hiranrat Darren C. Holland Wilawan Mahabusarakam John N. A. Hooper Vicky M. Avery Anthony R. Carroll 《Marine drugs》2021,19(2)
Two new fluorescent pteridine alkaloids, tedaniophorbasins A (1) and B (2), together with the known alkaloid N-methyltryptamine, were isolated, through application of mass directed purification, from the sponge Tedaniophorbas ceratosis collected from northern New South Wales, Australia. The structures of tedaniophorbasins A and B were deduced from the analysis of 1D/2D NMR and MS data and through application of 13C NMR DFT calculations. Tedaniophorbasin A possesses a novel 2-imino-1,3-dimethyl-2,3,7,8-tetrahydro-1H-[1,4]thiazino[3,2-g]pteridin-4(6H)-one skeleton, while tedaniophorbasin B is its 2-oxo derivative. The compounds show significant Stokes shifts (~14,000 cm−1) between excitation and emission wavelengths in their fluorescence spectra. The new compounds were tested for bioactivity against chloroquine-sensitive and chloroquine-resistant strains of the malaria parasite Plasmodium falciparum, breast and pancreatic cancer cell lines, and the protozoan parasite Trypanosoma brucei brucei but were inactive against all targets at 40 µM. 相似文献
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José F. Barbosa-Neto Wilawan Siripoonwiwat Louise S. O'Donoughue Stewart M. Gray Down M. Smith Fred L. Kolb Catherine Gourmet Charlie M. Brown Mark E. Sorrells 《Euphytica》2000,114(1):67-76
Immature embryos of the spring barley variety GoldenPromise, were bombarded with three different particledelivery systems and both transient and stabletransformation examined. In addition, a range oftechniques for the preparation of the DNA coated goldparticles was examined. Fertile transgenic barleyplants were obtained using three particle preparationtechniques which differed in the amount of gold andDNA used for each bombardment. However, only one ofthe particle delivery systems, the PDS 1000/He device,appeared to be effective in yielding transformedbarley plants. 相似文献
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