Comparative antigenic analysis of extracellular proteins of Bacteroides nodosus isolated from virulent and benign ovine footrot     

W K Yong  L M Gordon 《Veterinary microbiology》1986,12(2):135-145

Antigens in the extracellular protein (ECP) complexes of Bacteroides nodosus, isolated from sheep with either benign or virulent footrot, were studied by immunoelectrophoresis (IEP). Rabbit antisera against ECP from virulent and benign strains, were used in homologous and heterologous crossed IEP. Four precipitin peaks unique to the virulent strain, and five peaks unique to the benign strain were identified. In an attempt to characterize the different antigens in ECP, rabbit antisera were raised against an outer membrane protein (OMP, mol. wt. 35 000 daltons), pili and various proteases of virulent and benign strains of B. nodosus. No precipitin band was observed when ECP from both B. nodosus strains were reacted against anti-OMP and anti-pilus antisera. However, single precipitin bands unique to one protease from the benign strain and one protease from the virulent strain were identified. The results suggest that specific antigens other than proteases or pili are important in determining whether a B. nodosus isolate is virulent or benign.  相似文献   

Australian College of Veterinary Scientists     

HMcL Gordon 《Australian veterinary journal》1992,69(5):122-123

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Effect of porcine somatotropin on number of granulosa cell luteinizing hormone/human chorionic gonadotropin receptors, oocyte viability, and concentrations of steroids and insulin-like growth factors I and II in follicular fluid of lean and obese gilts.     

L J Spicer  J Klindt  F C Buonomo  R Maurer  J T Yen  S E Echternkamp 《Journal of animal science》1992,70(10):3149-3157

Prepubertal gilts of obese (n = 24) or lean (n = 24) genetic lines were injected (s.c.) daily with 0, 2, or 4 mg of porcine somatotropin (pST) for 6 wk starting at 160 d of age to determine whether pST affects follicular function. Blood and ovaries were collected at slaughter 24 h after the last injection. Surface follicles greater than or equal to 1.0 mm in diameter were counted, and pools of follicular fluid (FFL) and granulosa cells were collected from 1.0- to 3.9-mm (small) and 4.0- to 6.9-mm (medium) follicles. Oocytes were collected from small and medium follicles and evaluated for maturational stage and viability. Porcine somatotropin increased (P less than .08) the numbers of small but not the numbers of medium follicles per gilt (P greater than .10). Oocyte maturation and viability were not affected by pST or genetic line. Porcine somatotropin increased (P less than .05) concentrations of insulin-like growth factor I (IGF-I) in serum and FFL of both obese and lean gilts; IGF-I was lower (P less than .01) in lean gilts. Treatment with pST decreased (P less than .05) IGF-II in FFL of lean but not in that of obese gilts. Dose of pST and line had no effect on concentrations of progesterone in FFL of small or medium follicles or on concentrations of estradiol in FFL of small follicles.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Effects of ventilation and air temperature on pollution levels in underground car parks     

M. I. Yaziz  A. W. P. Yen 《Water, air, and soil pollution》1989,44(3-4):375-385

The relationship between the levels of CO and airborne Pb was evaluated against conditions of traffic flow, ambient air temperature and the ventilation rates in 3 underground car parks. During the study period significant variations were noted in the levels of the pollutants in each of the car parks which frequently exceeded the proposed Malaysian Air Quality Standard of 0.7 μg m^?3Pb and 9 μg L^?1 (8 hr average) CO for indoor environs. High correlation coefficient values were obtained between the levels of CO and traffic flow and Pb and traffic flow. High traffic flow also increased the mean air temperature inside the car parks. Reductions in the ventilation rates in the car parks caused an almost two-fold increase in the level of CO and vice versa. A simple mathematical model depicting the CO concentrations in relation to other parameters was formulated.  相似文献   

猪轮状病毒2型(PCV2)是潜在的“定时炸弹”吗?     

Gordon Allan  Steve Krakowka  John Ellis  王洪戈 《中国牧业通讯》2003,(20):57-58

对猪轮状病毒2型(PCV2)的研究,为全球探讨断奶后多系统消瘦综合征(PMWS)的特殊问题,提供了许多答案。但是本文提出了一些还需要特别注意的地方 ,用以避免其可能导致的“恶果”出现。过去几年里 ,在关于断奶后多系统消瘦综合征(PMWS)、猪轮状病毒病(PCVD )全球性的探讨中 ,已经对许多关于疾病流行特点的来源和特征进行了广泛的研究。猪血清中猪轮状病毒2型 (PCV2)抗体以及猪的组织样本的检测表明 ,至少从1969年起 ,猪轮状病毒2型 (PCV2)的抗体就已经存在于猪的群体中了。现在已经确定 ,从1986年起在西班牙和英国就发现了传统的PMWS…  相似文献   

Administration of porcine somatotropin by sustained-release implant: growth and endocrine responses in genetically lean and obese barrows and gilts.     

J Klindt  F C Buonomo  J T Yen 《Journal of animal science》1992,70(12):3721-3733

Previous studies have documented the effectiveness of porcine somatotropin (pST) administered by daily injection in promoting lean tissue growth in lean and obese pigs and the influence of sex and genotype. The present study examined the accretive responses in pigs of different lines and sexes to a slow release formulation of pST (pST-SR). Implants that deliver 2.0 mg of pST/d were implanted in genetically lean and obese barrows and gilts at 65 +/- .7 kg BW (mean +/- SE). Pigs received no, one, or two implants (i.e., doses of 0, 2.0, and 4.0 mg of pST/d). Pigs (four per line x sex x dose) were housed individually and continuously supplied with fresh water and a 19% CP diet containing 1.08% lysine. Pigs were slaughtered on d 0 (four per line x sex) and at the end of the trial (approximately 42 d after implantation) for estimation of initial composition and calculation of accretion rates. Blood samples were collected at d 0, 7, 14, 28, and 42 to measure endocrine and metabolite responses to pST-SR. Sustained-release pST elevated (P < .05) circulating pST throughout the trial with peak concentrations at d 7. On d 7, serum pST concentrations in the pigs given 2.0 mg of pST-SR per day were 16-fold greater than those in control pigs, and in pigs given 4.0 mg of pST-SR per day pST concentrations were 33-fold greater than in controls. Elevated serum pST resulted in increased (P < .05) serum concentrations of insulin-like growth factor (IGF)-I, IGF-II, insulin, and glucose and in reduced (P < .05) concentrations of urea nitrogen and IGF binding protein (IGFBP)-2. Gain was not influenced by pST-SR dose; however, feed consumption was reduced (P < .05) and efficiency of gain was increased (P < .05). Accretion of all body components except cold carcass weight, cecum, and untrimmed Boston butt and ham were changed (P < .05) with pST-SR administration. Heart and stomach were the only components of the carcass and offal whose accretion was not affected by line or sex. Increases in accretion of carcass components (< 75%) induced by sustained-release pST were considerably less than those measured in the organs (liver, 157%; lungs, 748%). The pST-SR treatment resulted in elevated serum concentrations of pST and its mediators and improved efficiency and composition of gain.(ABSTRACT TRUNCATED AT 400 WORDS)  相似文献   

Australian College of Veterinary Scientists     

Hugh McL Gordon 《Australian veterinary journal》1993,70(6):236-236

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Pigeon Health and Disease          下载免费PDF全文

Gordon A. Chalmers 《The Canadian veterinary journal. La revue veterinaire canadienne》1993,34(9):561

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Ⅱ型猪圆环病毒--滴答作响的定时炸弹?     

Gordon Allan  Steven Krakowka  John Ellis  李希林 《国外畜牧学(猪与禽)》2003,23(2):47-49

对Ⅱ型猪圆环病毒的研究为与猪断奶后多系统消耗性综合征在全球爆发有关的问题提供了大量答案,但还有一些方面迫切需要引起人们的关注,以便为可能出现的梦魇般的前景准备对策.  相似文献   

A 31P topical magnetic resonance study of embryonic development in hens' eggs     

P. S. Belton  R. E. Gordon  J. M. Jones  D. Shaw 《British poultry science》1983,24(3):429-433

1. A ³¹P topical magnetic resonance study of whole chicken's eggs in vivo is described.

2. Resonances from adenosine triphosphate, phosphocreatine, inorganic phosphate and phosphorous storage protein were observed. These changed in relative intensity as the embryo developed.

3. No ill effects due to exposure to radiofrequency radiation or magnetic fields were observed.

4. It is concluded that topical magnetic resonance can make a useful contribution to the study of embryonic development in avian eggs.  相似文献