Effects of three methionine sources in diets on temporal B0AT1 and ASCT2 mRNA expression in different intestinal segments of cobia,Rachycentron canadum     

Yuanfa He  Shuyan Chi  Beiping Tan  Xiaohui Dong  Qihui Yang  Hongyu Liu  Shuang Zhang 《Journal of the World Aquaculture Society》2021,52(1):171-183

A 4‐week feeding trial was conducted using five groups of hybrid grouper (61.15 ± 0.15 g) to explore the potential effects of three methionine (Met) sources. Five isonitrogenous and isolipidic diets were formulated that included a fishmeal (FM) diet; nonsupplemented (NS) diet; or NS diets with the addition of the L‐methionine (L‐Met), DL‐methionine (DL‐Met), or coated Met (Co‐Met) to obtain the same Met level as the FM diet. Fish were randomly distributed into sea cages (30 fish per cage). Weight gain and specific growth rate in the DL‐Met group and FM group were significantly higher than those in all other groups (p < .05). In the proximal and distal intestines, of the 20 gene and time combinations (10 per gene), there were 14 combinations (70%) in which there were no significant differences in gene expression levels between the FM and DL‐Met groups (p > .05). The main reason for the same growth effect between the DL‐Met and FM groups may be attributed to the synchronized absorption at most time points after feeding, which had similar expression patterns of B⁰AT1 and ASCT2 of the proximal and distal intestines between two groups.  相似文献   

大枫子与马钱上的叶斑类真菌病害   总被引：1，自引：1，他引：0  

林石明  戚佩坤 《热带作物学报》1992,13(2):97-102

报道1986～1989年在泰国大枫子、海南大枫子和印度马钱上发现的9种叶斑类真菌病害的症状、病原菌及病情、分布。9种病原菌中,大枫子褐斑病菌(Ascochyta hydnocarpi S.M.Lin et P.K.Chi)、马钱叶枯病菌(Didymosphaeria strychnoris S.M.Lin et P.K.Chi)、马钱叶斑病菌(Microsphaeropsis strychoris S.M.Lin et P.K.Chi)和马钱轮纹褐斑病菌(Mycosphaerella strychnoris S.M.Lin et P.K.Cki)是新种。  相似文献   

Effect of porcine somatotropin on number of granulosa cell luteinizing hormone/human chorionic gonadotropin receptors, oocyte viability, and concentrations of steroids and insulin-like growth factors I and II in follicular fluid of lean and obese gilts.     

L J Spicer  J Klindt  F C Buonomo  R Maurer  J T Yen  S E Echternkamp 《Journal of animal science》1992,70(10):3149-3157

Prepubertal gilts of obese (n = 24) or lean (n = 24) genetic lines were injected (s.c.) daily with 0, 2, or 4 mg of porcine somatotropin (pST) for 6 wk starting at 160 d of age to determine whether pST affects follicular function. Blood and ovaries were collected at slaughter 24 h after the last injection. Surface follicles greater than or equal to 1.0 mm in diameter were counted, and pools of follicular fluid (FFL) and granulosa cells were collected from 1.0- to 3.9-mm (small) and 4.0- to 6.9-mm (medium) follicles. Oocytes were collected from small and medium follicles and evaluated for maturational stage and viability. Porcine somatotropin increased (P less than .08) the numbers of small but not the numbers of medium follicles per gilt (P greater than .10). Oocyte maturation and viability were not affected by pST or genetic line. Porcine somatotropin increased (P less than .05) concentrations of insulin-like growth factor I (IGF-I) in serum and FFL of both obese and lean gilts; IGF-I was lower (P less than .01) in lean gilts. Treatment with pST decreased (P less than .05) IGF-II in FFL of lean but not in that of obese gilts. Dose of pST and line had no effect on concentrations of progesterone in FFL of small or medium follicles or on concentrations of estradiol in FFL of small follicles.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

近似法计量施用有机肥料对培肥水稻土土壤的研究     

孙丽宏  王长青  林立萍  迟成凯 《北方水稻》2006,(6):60-62

用近似法计量施用有机肥培肥土壤,测定土壤有机质矿化率和农肥及作物根茬的腐殖化系数。5a试验结果表明,土壤有机质矿化率在轻壤质良水型水稻土上平均为2.44%,轻壤质滞水型水稻土上平均为1.39%。有机质的腐殖化系数:马粪为35%～40%;猪粪为28%～34%;水稻根茬为45%。根据上述结果,计算了有机肥的建议施肥量,该计算值在水稻土上基本符合生产实际情况。  相似文献   

Effects of ventilation and air temperature on pollution levels in underground car parks     

M. I. Yaziz  A. W. P. Yen 《Water, air, and soil pollution》1989,44(3-4):375-385

The relationship between the levels of CO and airborne Pb was evaluated against conditions of traffic flow, ambient air temperature and the ventilation rates in 3 underground car parks. During the study period significant variations were noted in the levels of the pollutants in each of the car parks which frequently exceeded the proposed Malaysian Air Quality Standard of 0.7 μg m^?3Pb and 9 μg L^?1 (8 hr average) CO for indoor environs. High correlation coefficient values were obtained between the levels of CO and traffic flow and Pb and traffic flow. High traffic flow also increased the mean air temperature inside the car parks. Reductions in the ventilation rates in the car parks caused an almost two-fold increase in the level of CO and vice versa. A simple mathematical model depicting the CO concentrations in relation to other parameters was formulated.  相似文献   

Administration of porcine somatotropin by sustained-release implant: growth and endocrine responses in genetically lean and obese barrows and gilts.     

J Klindt  F C Buonomo  J T Yen 《Journal of animal science》1992,70(12):3721-3733

Previous studies have documented the effectiveness of porcine somatotropin (pST) administered by daily injection in promoting lean tissue growth in lean and obese pigs and the influence of sex and genotype. The present study examined the accretive responses in pigs of different lines and sexes to a slow release formulation of pST (pST-SR). Implants that deliver 2.0 mg of pST/d were implanted in genetically lean and obese barrows and gilts at 65 +/- .7 kg BW (mean +/- SE). Pigs received no, one, or two implants (i.e., doses of 0, 2.0, and 4.0 mg of pST/d). Pigs (four per line x sex x dose) were housed individually and continuously supplied with fresh water and a 19% CP diet containing 1.08% lysine. Pigs were slaughtered on d 0 (four per line x sex) and at the end of the trial (approximately 42 d after implantation) for estimation of initial composition and calculation of accretion rates. Blood samples were collected at d 0, 7, 14, 28, and 42 to measure endocrine and metabolite responses to pST-SR. Sustained-release pST elevated (P < .05) circulating pST throughout the trial with peak concentrations at d 7. On d 7, serum pST concentrations in the pigs given 2.0 mg of pST-SR per day were 16-fold greater than those in control pigs, and in pigs given 4.0 mg of pST-SR per day pST concentrations were 33-fold greater than in controls. Elevated serum pST resulted in increased (P < .05) serum concentrations of insulin-like growth factor (IGF)-I, IGF-II, insulin, and glucose and in reduced (P < .05) concentrations of urea nitrogen and IGF binding protein (IGFBP)-2. Gain was not influenced by pST-SR dose; however, feed consumption was reduced (P < .05) and efficiency of gain was increased (P < .05). Accretion of all body components except cold carcass weight, cecum, and untrimmed Boston butt and ham were changed (P < .05) with pST-SR administration. Heart and stomach were the only components of the carcass and offal whose accretion was not affected by line or sex. Increases in accretion of carcass components (< 75%) induced by sustained-release pST were considerably less than those measured in the organs (liver, 157%; lungs, 748%). The pST-SR treatment resulted in elevated serum concentrations of pST and its mediators and improved efficiency and composition of gain.(ABSTRACT TRUNCATED AT 400 WORDS)  相似文献   

松口蘑的研究进展   总被引：2，自引：0，他引：2  

邹莉  李玲  池玉杰 《中国食用菌》2005,24(3):11-13

详细论述了松口蘑的生物学特性、栽培技术及液体深层发酵技术，并探讨了过氧化物酶和酯酶同工酶技术、原生质体技术、基因工程技术与DNA指纹技术在松口蘑研究中的应用及发展趋势。  相似文献   

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats          下载免费PDF全文

Sui Zhi Tian  Chai Ju Chang  Chih Chi Chiang  Huo Cheng Peh  Mu Chiou Huang  Jai-Wei Lee    Xin Zhao 《Canadian journal of veterinary research》2005,69(1):39-45

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.  相似文献   

亚热带牧用草地中威提特东非狼尾草的种子繁殖研究   总被引：4，自引：0，他引：4  

匡崇义 徐驰薛世明  吴文荣 《四川草原》2004,(10):8-10

在3月和4月对全日制放牧的东非狼尾草草地上的风干牛粪取样,进行发芽试验,分别测定其中的东非狼尾草发芽种子数量。结果,3月取样平均432.5 g的牛粪中东非狼尾草平均发芽种子数量为118.25粒,4月取样平均437.8 g的牛粪中东非狼尾草平均发芽种子数量为52.8粒,二者平均牛粪重为435.15 g,平均发芽种子为85.5粒,说明牛粪中东非狼尾草种子数量较多,是其繁殖扩展较快的主要原因;3月取样的种子数量为4月取样数量的1.38倍,说明人工利用牛粪进行东非狼尾草繁殖时,需在有降雨来临前准备,即1～3月进行牛粪的准备工作。  相似文献   

在放牧干扰下冷蒿种群脯氨酸含量的变化     

王静  杨持 《中国草地学报》2005,27(4):52-57

对放牧干扰下土壤含水量、冷蒿体内含水量、脯氨酸含量变化进行的研究结果表明随着放牧强度的增加土壤含水量降低,冷蒿叶片、根中含水量呈下降趋势。在生长初期,随着放牧强度的增加冷蒿叶片内脯氨酸含量迅速积累;生长盛期脯氨酸含量虽随牧压有所增加,但增加的幅度较小;生长末期脯氨酸含量较高,而且随着牧压的增加略有下降。地下部分,脯氨酸含量在牧压梯度间变化较小。相关分析表明,在生长初期和盛期,冷蒿叶片、根的含水量与土壤含水量呈正相关,生长末期不相关。冷蒿脯氨酸含量与植物的含水量呈负相关,在生长初期和末期呈显著负相关,而生长盛期相关不显著;与此不同,在生长初期冷蒿体内脯氨酸含量与土壤含水量呈负相关,在生长末期二者负相关性不显著,这说明随着放牧干扰强度的增加土壤含水量降低,导致冷蒿体内水分胁迫增加,致使脯氨酸开始逐渐积累。而生长末期的降温和群落中植物种间竞争减少,使脯氨酸的积累速度也相应放慢。在放牧干扰下,脯氨酸大量积累,对冷蒿的生存和生长起到了重要的作用,是冷蒿种群耐啃食、耐践踏、逐渐取代禾本科植物而成为退化草原的优势植物的生理基础之一。  相似文献