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Effects of Body Condition and Protein Supplementation on LH Secretion and Luteal Function in Sheep 总被引:1,自引:0,他引:1
CA Meza-Herrera T Ross D Hallford D Hawkins A Gonzalez-Bulnes 《Reproduction in domestic animals》2007,42(5):461-465
In ruminants, nutrition is one of the exogenous inputs affecting reproductive function at different levels of the hypothalamic-hypophyseal-gonadal axis. However, the exact mechanisms or even the identification of the signalling metabolic compounds by which nutrition affects reproductive function still need further clarification. The role of static body condition (BC) and its interaction with a short-term protein supplementation (PL), on secretion of metabolic hormones [growth hormone (GH), insulin and insulin-like growth factor-1 (IGF-1)], as well as on secretion of LH and progesterone (P4) was evaluated in sheep. Twenty-four Rambouillet ewes divided into two groups, with lower (LBC) and higher body condition (HBC), were randomly assigned within BC to one of two PL levels: low (LPL, 24% of crude protein; 14 g/animal/day), and high (HPL, 44% of crude protein; 30 g/animal/day). The secretion of GH, insulin, IGF-1 and LH was evaluated on day 10 of the oestrous cycle; appearance and timing of oestrous behaviour were previously detected using rams. Progesterone secretion was evaluated on day 13 of the same cycle. No differences were found (p > 0.05) between PL groups on serum GH concentrations during the sampling period (overall mean of 4.0 +/- 0.3 ng/ml), but a trend for lower values in HBC sheep was found (3.6 +/- 0.4 vs 4.4 +/- 0.4 ng/ml, p = 0.06). A BC effect was observed (p < 0.05) on serum IGF-1 level, with higher values in HBC sheep (p < 0.05). Neither BC nor PL affected (p > 0.05) secretion of LH and the number of corpora lutea, nor serum P4 and insulin concentrations. Results indicate a predominance of the static component of nutrition on sheep metabolic hormone responses, GH and IGF-1, with no effect of short-term PL on secretion of pituitary and ovarian hormones as well as luteal number and activity. 相似文献
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Follicular Dynamics in Heifers during Pre-pubertal and Pubertal Period Kept under Two Levels of Dietary Energy Intake 总被引:1,自引:0,他引:1
MA Romano VH Barnabe JP Kastelic CA de Oliveira RM Romano 《Reproduction in domestic animals》2007,42(6):616-622
The objective of this study was to characterize follicular dynamics in pre-pubertal, pubertal and post-pubertal periods, as well as the effect of high-energy intake on follicular development and age at puberty in heifers. Thirty-one Nelore (Bos indicus) heifers, 6 months old, were randomly assigned to receive two different diets: one of low (GI) and other of high dietary energy intake (GII). Animals were evaluated in relation to body weight gain by being weighed every 21 days. Heifers were evaluated every other day by real-time linear ultrasonography to characterize ovarian structures development from weaning to post-pubertal period. Blood samples were collected to determine plasmatic concentrations of progesterone by RIA method. The ovulation was determined when progesterone concentrations were >1 ng/mL in three consecutive samples, and by ultrasound images of corpus luteum; and oestrous behaviour in some animals. Age at puberty differed among heifers of GII (17.00 +/- 0.46 months) compared with heifers of GI (19.87 +/- 0.47 months; p < or = 0.05). Maximum size of the dominant follicles at pre-pubertal period was greater in GII heifers than in GI (10.52 +/- 0.33 and 9.76 +/- 0.15 mm, respectively; p < or = 0.05). As heifers approached first ovulation time, size of dominant follicle increased (11.75 +/- 0.37 mm for GI and 12.52 +/- 0.91 mm for GII; p < or = 0.05). Body weight at puberty was not different in both groups (302.33 +/- 27.31 kg for GI and 326.19 +/- 27.78 kg for GII heifers; p > 0.05). We conclude that animals receiving high dietary energy intake attained the puberty earlier and the development of follicles were different than in low dietary energy intake. 相似文献
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本文利用相关系数(r)和剩余标准差(SD),探讨了6种不同数学模型估测柏木人工林现存量的效果。发现方程W=a+b_1D+b_2D~1+b_3DH+b_4H+b_5H~2是估测干材、树皮、枝、叶、根、地上部分、地下部分和全株现存量的最优模型,而且普遍适用于不同立地类型、林型和年龄的林分。并且对不同器官估测效果优劣进行了比较,结论是:干材>枝>叶>树皮>根。 相似文献
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Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality 下载免费PDF全文
F Krania E Dovolou CA Rekkas EK Theodosiadou I Pappas GS Amiridis 《Reproduction in domestic animals》2015,50(1):112-120
Plasminogen activators/Plasmin system plays pivotal role in regulating reproductive functions of mammals. Here, we examined the effects of modification of in vitro fertilization medium (IVF medium) with the addition of tissue‐type plasminogen activator (t‐PA), on bovine embryo development and quality, assessed by quantification of expression of various genes related to metabolism, oxidation, implantation and apoptosis. In addition, plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in the spent media. After conventional IVM, 2016 cumulus‐oocyte complexes (COCs) were divided into four groups with modified composition of the IVF medium containing t‐PA and/or its inhibitor epsilon‐aminocaproic acid (control, t‐PA, t‐PA+ε‐ACA, ε‐ACA). Presumptive zygotes were cultured for 8 days in synthetic oviductal fluid (SOF) medium; gene expression studies were carried out on morulae and blastocysts. t‐PA alone significantly suppressed cleavage and blastocyst formation rates, but this effect was neutralized by the addition of ε‐ACA. PAA in the treated group was significantly reduced by ε‐ACA, but without total elimination. Significant differences were detected in the expression of genes related to apoptosis and/or cell cycle arrest (BAX, BCL2L1, KAT2B) between embryos produced in t‐PA‐modified media and controls, giving an overall notion that the inferior developmental competence of treated embryos may be attributed to apoptotic phenomena induced by t‐PA. In conclusion, it appears that excessive t‐PA content in the IVF media, suppresses blastocyst formation rate, possibly due to induction of apoptotic phenomena. 相似文献
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为研究不同基肥及追肥配比对凤梨生长、产量及果实品质的影响,以台农17凤梨为研究对象,分别设基肥试验和追肥试验,明确最佳的凤梨施肥模式。基肥试验设复合肥、“劲久”控释肥、羊粪+复合肥和“沃丰康”生物有机肥+复合肥4种肥料组合,每种组合根据总氮含量设100% N、75% N与50% N三种梯度,其中不添加基肥为CK1,共计13个基肥处理。追肥试验设T1(促花肥+开花肥+结果肥)、T2(开花肥+结果肥)、T3(促花肥+结果肥),其中不施用追肥为CK2,共4个追肥处理。结果表明,在100% N水平下,以11 250 kg·hm-2羊粪+1 875 kg·hm-2复合肥作为基肥,凤梨的生长、产量及果实品质最佳。移栽135 d后,凤梨的叶尖高度增加了28.25%,总叶片数增加59.09%,最大叶长增加16.00%,最大叶宽增加42.55%。凤梨产量达58.62 t·hm-2,较CK1增加20.22%,平均单果重达(1.86±0.32)kg, 果长、果径与芯径分别达(231.47±44.28)mm、(125.62±33.24)mm和(41.16±4.13)mm。此外凤梨的果实品质也最佳,果肉可溶性固形物达(12.6±0.4)%,可滴定酸达(0.50±0.11)%,维生素C达(29.1±6.7)mg·kg-1。而在75%N水平或50%N水平下,“沃丰康”生物有机肥+复合肥处理的部分凤梨指标更佳。作为追肥,T1的效果最佳,较于追肥前,追肥后的凤梨叶尖高度、总叶片数、最大叶长叶宽分别增加20.18%、184.87%、21.95%和61.25%,产量较CK2增加6.62%。凤梨单果重(1.62±0.33)kg,果长、果径和芯径分别达(183.52±29.35)mm、(123.58±15.68)mm和(40.58±8.39)mm。凤梨果肉可溶性固形物(13.1±0.9)%,可滴定酸(0.47±0.12)%,维生素C含量(28.9±3.2)mg·kg-1。本研究表明,适用于宁波本地凤梨的最佳施肥方式为以11 250 kg·hm-2羊粪+1 875 kg·hm-2复合肥作为基肥,同时添加少量生物有机肥,并辅以促花肥、开花肥和结果肥追施。 相似文献
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