Conductive 3D structure nanofibrous scaffolds for spinal cord regeneration     

Fatemeh Zamani  Mohammad Amani-Tehran  Arash Zaminy  Mohammad-Ali Shokrgozar 《Fibers and Polymers》2017,18(10):1874-1881

The complex nature of spinal cord injuries has provided much inspiration for the design of novel biomaterials and scaffolds which are capable of stimulating neural tissue repair strategies. Recently, conductive polymers have gained much attention for improving the nerve regeneration. In our previous study, a three-dimensional (3D) structure with reliable performance was achieved for electrospun scaffolds. The main purpose in the current study is formation of electrical excitable 3D scaffolds by appending polyaniline (PANI) to biocompatible polymers. In this paper, an attempt was made to develop conductive nanofibrous scaffolds, which can simultaneously present both electrical and topographical cues to cells. By using a proper 3D structure, two kinds of conductive scaffolds are compared with a non-conductive scaffold. The 3D nanofibrous core-sheath scaffolds, which are conductive, were prepared with nanorough sheath and aligned core. Two different sheath polymers, including poly(lactic-co-glycolic acid) PLGA and PLGA/PANI, with identical PCL/PANI cores were fabricated. Nanofibers of PCL and PLGA blends with PANI have fiber diameters of 234±60.8 nm and 770±166.6 nm, and conductivity of 3.17×10^-5 S/cm and 4.29×10^-5 S/cm, respectively. The cell proliferation evaluation of nerve cells on these two conductive scaffolds and previous non-conductive scaffolds (PLGA) indicate that the first conductive scaffold (PCL/ PANI-PLGA) could be more effective for nerve tissue regeneration. Locomotor scores of grafted animals by developed scaffolds showed significant performance of non-conductive 3D scaffolds. Moreover, the animal studies indicated the ability of two new types of conductive scaffolds as spinal cord regeneration candidates.  相似文献   

Prevalence of avian influenza (H9N2) in commercial quail,partridge, and turkey farms in Iran, 2014–2015     

Mehrabadi  Mohammad Hosein Fallah  Bahonar  Alireza  Mirzaei  Kamran  Molouki  Aidin  Ghalyanchilangeroudi  Arash  Ghafouri  Seyed Ali  Tehrani  Farshad  Lim  Swee Hua Erin 《Tropical animal health and production》2018,50(3):677-682

Tropical Animal Health and Production - Avian influenza virus (AIV) H9N2 subtype is endemic in Iran and causes substantial economic loss to the growing poultry industry within the country. In this...  相似文献   

Low potassium diets with different levels of calcium in comparison with different anionic diets fed to prepartum dairy cows: Effects on sorting behaviour,total tract digestibility,energy metabolism,oxidative status and hormonal response     

Abbas Rajaeerad  Gholam R. Ghorbani  Mohammad Khorvash  Ali Sadeghi-Sefidmazgi  Amir H. Mahdavi  Mirja R. Wilkens 《Journal of animal physiology and animal nutrition》2021,105(1):14-25

This study investigated the effects of low potassium diets with different levels of Ca compared to two diets low in dietary cation–anion difference (DCAD) fed prepartum as a strategy to prevent hypocalcemia on sorting behaviour, total tract digestibility, oxidative status and energy and protein metabolism of transition cows. Forty-eight pregnant dairy cows were assigned to 4 treatment groups: Low Ca, low K (LCLK), High Ca, low K (HCLK), Supplementation with anionic mineral mixture (AMS) supplementation with SoyChlor (CAS). After parturition, all animals were fed a standard postpartum diet. Data were collected until 21 DIM. Prepartum urinary pH was significantly reduced by the low DCAD diets, while postpartum Ca homeostasis was affected by the HCLK ration. Feeding AMS induced sorting against particles <1.18 mm in favour of particles >19 mm prepartum. In contrast, cows fed CAS showed an increase in selective consumption of fine particles and sorted against longer particles similar to the HCLK and LCLK groups. Postpartum sorting activity was not affected by the dietary treatments. After calving, apparent digestibility of NDF was significantly reduced in the HCLK group. Prepartum, we observed effects on serum concentrations of non-esterified fatty acids were higher and insulin sensitivity was lower in the AMS group. Blood urea nitrogen (BUN) was decreased in cows fed the CAS ration. Postpartum, we found serum protein to be decreased with the low DCAD diets while BUN was decreased in the CAS group. The low DCAD rations increased prepartum serum malondialdehyde concentrations, while postpartum total antioxidant capacity was lower in the HCLK and the AMS group. From these data, we conclude that AMS decreased prepartum intake due to compromised palatability. Intermediate protein metabolism was affected by the low DCAD diets, while parameters of oxidative stress were probably affected by acid–base balance and Ca homeostasis.  相似文献   

The effects of transportation stress on Japanese quail (Coturnix Coturnix japonica) fed corn‐based diet in comparison with wheat‐based diet supplemented with xylanase and phytase          下载免费PDF全文

M. H. Mehraei Hamzekolaei  A. K. Zamani Moghaddam  S. S. Tohidifar  A. Dehghani Samani  A. Heydari 《Journal of animal physiology and animal nutrition》2016,100(4):618-622

Harvesting, handling and transporting quails to the slaughterhouses, other farms and laboratories might covertly reduce their welfare. The aim of this study was to evaluate the effects of two major sources of energy in poultry nutrition on reducing transportation stress in Japanese quail (Coturnix Coturnix japonica). Male quails (n = 60) were divided into two groups. The first group was fed corn‐based diet, and the second was fed wheat‐based diet supplemented with xylanase and phytase. At the end of the experiment (day 35), quails were subjected to 80 km of transportation. Immediately on arrival and after 24 h, heterophil counts, lymphocyte counts and H:L ratios were measured. On arrival, H counts were lower, L counts were higher, and H:L ratios were lower for corn‐fed group. After 24 h, wheat‐fed group showed lower increment of H counts, greater increment of L counts and also decrement of H:L ratios rather than corn‐fed group which showed increment of H:L ratios. However, these ratios were still lower in corn‐fed group. Results indicate that corn‐based diets can help Japanese quail to better resist transportation stress, although it seems that feeding wheat‐based diets supplemented with xylanase and phytase could have positive effects for coping better with stress after journeys.  相似文献   

Farmer participation in irrigation management: The case of Doroodzan Dam Irrigation Network, Iran   总被引：1，自引：0，他引：1  

Taher Azizi Khalkheili  Gholam Hosein Zamani 《Agricultural Water Management》2009,96(5):859-865

The importance of farmer participation in system design and management has been emphasized in previous studies. The purpose of this study was to identify the factors affecting farmer participation in irrigation management using survey research. The study was conducted in Doroodzan Dam Irrigation Network in Fars province, Iran. Multistage stratified random sampling was used to collect data from 270 farmers as the research sample. Results reveal that farmers’ attitudes toward participation in irrigation management, attitudes toward personnel of the State Water Authority and the Agricultural Extension Service Centers (AESCs), family size, the problem perception, dependence on the dam for water, and educational background have influenced their participation in irrigation management. By contrast, contact with information sources, animal units, sociability, age and agricultural experience did not affect farmers’ participation. Moreover, based on farmers’ perspectives, unequal water distribution among farms, dissatisfaction with Water Authority operators and high water fees and charges were the main problems and obstacles toward farmer participation in irrigation management.  相似文献   

Designing E1 deleted adenoviral vector by homologous recombination   总被引：2，自引：0，他引：2  

Zamani A  Ghannad MS 《Iranian Biomedical Journal》2007,11(3):199-202

BACKGROUND: Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generation of large collection of clones. METHODS: In this project, to delete E1 gene in adenovirus, an adenoviral plasmid containing lateral sites of E1 region of adenovirus was made and recombination in the 293A cells between the homologous region of this linearized plasmid and the adenovirus genome resulted in the formation of the complete adenoviral recombinant. RESULTS: This recombination resulted in loss of E1 region and we constructed a recombinant adenovirus type 5 vector that E1 gene was deleted by homologous recombination. CONCLUSION: Homologous recombination is more easy and fast technique in the production of AdV.  相似文献   

Karyological analysis of endangered Caspian salmon, Salmo trutta caspius (Kessler, 1877)     

Mohammad Reza Kalbassi  Salar Dorafshan  Tahereh Tavakolian  Mahmoud Khazab  & Hosein Abdolhay 《Aquaculture Research》2006,37(13):1341-1347

The karyotype and chromosomal characteristics of endangered Caspian salmon, Salmo trutta caspius, in the southern part of the Caspian Sea were investigated. From the total of 60 mitotic metaphases achieved from 15 individuals, 15, 9 and 36 metaphases were with the mode <76, 76 and 80 representing 25%, 15% and 60% of metaphases respectively. So, the most common pattern of chromosome number was 80 (36 metaphases, 60%) and the number of diploid chromosomes was thus confirmed as (2n=80) of this subspecies. The karyotype consists of seven metacentric, five submetacentric and 28 telocentric pairs. The karyological parameters of the Caspian salmon, the centromic index, arm ratio, relative length and length variation range of chromosomes, were 0–0.5, 1–∞, 0.011–0.045 and 0.507–2.028 μm respectively. The total length of chromosomes in haploid series was 44.776 μm and fundamental number of chromosome arms was 104. This study may provide the first knowledge on chromosome analysis in Caspian salmon and add basic information useful for its chromosomal manipulations.  相似文献   

Effects of NaCl salinity on some leaf nutrient concentrations,non-photochemical quenching and the efficiency of the PSII photochemistry of two Iranian pomegranate varieties under greenhouse and field conditions: Preliminary results     

Mehdi Khayyat  Ali Tehranifar  Gholam Hossien Davarynejad  Mohammad Hassan Sayyari-Zahan 《Journal of plant nutrition》2016,39(12):1752-1765

The present research was conducted to study the responses of ‘Malas–e–Saveh’ (M) and ‘Shishe–Kab’ (Sh) Iranian pomegranates to sodium chloride (NaCl) stress under greenhouse and field conditions. Treatments included waters electrical conductivity (EC = 1.5, 3, 6, 9 and 12 dS m^?1 for greenhouse) and (EC = 1.05 as control, 4.61 and 7.46 dS m^?1 for field studies). Interactive effects of salinity × variety indicated the highest chlorophyll and leaf potassium concentration, and the lowest leaf chloride and sodium in control under greenhouse study. Non-photochemical quenching, effective quantum yield of photochemical energy conversion in PSII reduced under the highest salinity level in field, however, basal quantum yield of non-photochemical processes in PSII increased in the highest salinity. Sodium and chloride increased with increased in salinity. Calcium, magnesium and iron significantly decreased with increased in salinity. It seems that there are differences between pomegranate cultivars and Malas-e-Saveh is more tolerant compared with Shishe Kab.  相似文献   

Long-term high-titer neutralizing activity induced by octameric synthetic HIV-1 antigen   总被引：10，自引：0，他引：10  

C Y Wang  D J Looney  M L Li  A M Walfield  J Ye  B Hosein  J P Tam  F Wong-Staal 《Science (New York, N.Y.)》1991,254(5029):285-288

A titer for homologous viral neutralization activity (greater than 1:19,683) was observed after a 3.5-year immunization period with an octameric, branching peptide representing the principal neutralizing determinant (PND) of the human immunodeficiency virus-1IIIB envelope protein. Booster immunizations elicited persistent and potent antibodies in guinea pigs, exceeding responses produced by a conventional bovine serum albumin conjugate by 100-fold. Peptide length, central presentation of a conserved sequence, and inclusion of an upstream sequence contributed to immunogenicity. Titers (greater than 1:1,000) of heterotypic neutralizing antibodies also developed. Octameric PND peptides are a promising approach for an acquired immunodeficiency syndrome (AIDS) vaccine.  相似文献   

Identification and characterization of genes differentially displayed in Rosa hybrida petals during flower senescence     

Hanife Hajizadeh  Khadijeh Razavi  Younes Mostofi  Amir Mousavi  Giovanni Cacco  Zabihollah Zamani  Piergiorgio Stevanato 《Scientia Horticulturae》2011

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