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1.
The purpose of this study was to determine whether there were differences in skeletal muscle properties in the hindlimb muscles of different types of dogs. Muscle samples were obtained from the gracilis, sartorius cranial head, sartorius caudal head and tibialis anterior muscles of mixed-breed and hound-type dogs and Beagles. Fiber type, fiber size and capillary morphometry determinations of each muscle from each dog were made from sections stained for myofibrillar ATPase activity. Individual animals were bilaterally symmetric for all measured variables. Fiber type, fiber size and capillary geometry varied between dogs of a given type and muscles within a given dog. There were no differences between dog types for fiber type or fiber size; significant variation in log(muscle)/log(body) mass ratios between dog types was observed for all muscles. The results indicate that for a given muscle, significant variation can occur in skeletal muscle characteristics between different types of dogs and that these differences can be independent of differences in exercise history.  相似文献   
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The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding.  相似文献   
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Intervertebral 7.5 mm (n = 75) and 6.0 mm (n = 22) metal washers were used to distract intervertebral spaces in 78 consecutive cases of canine cervical spondylopathy-associated disc protrusions, where myelography had demonstrated traction-responsive spinal cord compression. Nineteen dogs had compressive lesions at two sites. Fifty lesions were evaluated myelographically on immediate postoperative radiographs; of these, 32 compressions had been eliminated and 18 had been reduced. Euthanasia was performed in nine dogs within six months of surgery and 15 dogs had varying degrees of neck pain during this period. Long-term follow-up information was available on 65 dogs nine to 70 months following surgery (median 32 months). Sixty-three of these dogs improved postoperatively. Neurological function subsequently deteriorated in 17 dogs 10 to 59 months following surgery (median 34 months). Eight of these 17 cases had further myelographic investigations and all had additional disc protrusions with no evidence of cord compression at the previous sites of surgery. The remaining nine cases had a deterioration in hindlimb function but were not investigated further.  相似文献   
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Rainfall, throughfall and stemflow were measured and canopy interception loss was derived for 14 rainfall events from June 22 to August 30, 2008 within a mature declining hybrid white spruce(Picea glauca(Moench) Voss × P. engelmannii Parry ex Engelm.)- subalpine fir(Abies lasiocarpa(Hook.) Nutt.)- lodgepole pine(Pinus contorta var.latifolia Dougl. ex Loud.) stand in south-central British Columbia, Canada. Stemflow was negligible during the study period, while, respectively throughfall and canopy interception loss accounted for approximately59.4% and 40.6% of the 50.1 mm of cumulative rainfall. Throughfall variability was assessed with three approaches involving roving and stationary wedge-type gauges, and stationary trough gauges. Throughfall exhibited large spatial variability with the coefficient of variability of study period throughfall sampled using 16 stationary trough gauges being30.3%, while it was 38.0% and 28.7% for 32 stationary and 32 roving wedge gauges, respectively. Our analysis suggests that a roving gauge method is better than a stationary approach since the errors associated with event mean throughfalls are summed quadratically and a greater portion of the canopy area is sampled. Trough gauges were more efficient than wedge gauges; however, this efficiency was less than expected given their much larger sampling areas, suggesting that spatial autocor-relation lengths of throughfall may be longer than the trough systems. The spatial distribution of throughfall showed a high degree of temporal persistence throughout the study suggesting the existence of stable "wet" and "dry" inputs to the floors of these coniferous forests.  相似文献   
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Ossification of the infraspinatus tendon-bursa was diagnosed in 13 labrador retrievers, 12 of which were lame in one thoracic limb and the other in both. They ranged in age from 28 to 121 months (mean 69.4 months). The lameness developed gradually and was progressive in 11 of the 14 affected joints. Scapular muscle atrophy and signs of pain on direct pressure over the infraspinatus tendon of insertion were key clinical signs. Caudocranial radiographs revealed multiple mineralised masses lateral to the proximal humerus or glenohumeral joint in 11 of the 26 joints and single masses in 12. An arthroscopic examination revealed concomitant ligament or tendon abnormalities in six of seven shoulders. The dogs were followed up from one to 55 months (mean 20 months). Of five shoulders treated with non-steroidal anti-inflammatory drugs (nsaids), one resolved, two improved and two were managed surgically. Of six shoulders treated by the injection of long-acting intra-articular corticosteroid (five before and one after surgery), three resolved, two improved and one was unchanged. Of six shoulders treated by the surgical resection of the infraspinatus tendon and bursa (three before and two after treatment with nsaids, and one after treatment with a long-acting intra-articular corticosteroid), four improved, one was unchanged and one was managed with an intra-articular long-acting corticosteroid. One shoulder was managed by restricted exercise and the lameness resolved. Histological examination of the excised tissues revealed heterotopic bone within the infraspinatus tendon and/or bursa.  相似文献   
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G. W. Douhan  E. Fuller  B. McKee  E. Pond 《Euphytica》2011,182(2):209-217
Phytophthora cinnamomi, the causal agent of Phytophthora Root Rot (PRR) of avocado, is the most serious disease of avocado worldwide. The development of tolerant rootstocks to control PRR has proven to be an effective means to control the disease. However, using traditional breeding approaches can take over a decade to produce results and there has been a limited use to integrate molecular data into breeding efforts. Therefore, the objective of this work was to use amplified fragment length polymorphism (AFLP) to genetically characterize greenhouse selected PRR tolerant rootstocks, some that had been field-tested and others that had not. Sixty-one polymorphic markers were identified among the 83 cultivars/selections tested. Based on UPGMA analysis, extensive genotypic diversity was found with the closest genotypes differing by six markers. No obvious trends were observed based on the cluster analysis with regards to known tolerant and susceptible rootstocks but rootstocks that have been field-tested and determined to be very tolerant rootstocks such as Latas, Dusa, PP14 (Uzi), PP4 (Zentmyer), and PP24 (Steddom) were distributed across the tree. This suggests that the same mechanisms for resistance may not be shared among these various selections. Therefore, these best-performing and most genetically diverse rootstocks will be combined in future breeding efforts to potentially pyramid diverse sources of resistance into future selections.  相似文献   
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