Analgesic efficacy of an oral transmucosal spray formulation of meloxicam alone or in combination with tramadol in cats with naturally occurring osteoarthritis          下载免费PDF全文

Beatriz P Monteiro  Mary P Klinck  Maxim Moreau  Martin Guillot  Paulo VM Steagall  Daniel K Edge  Jean‐Pierre Pelletier  Johanne Martel‐Pelletier  Dominique Gauvin  Jérôme RE del Castillo  Eric Troncy 《Veterinary anaesthesia and analgesia》2016,43(6):643-651

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Expression Pattern of Apoptotic Genes in Vitrified‐Thawed Bovine Oocytes     

VM Anchamparuthy  RE Pearson  FC Gwazdauskas 《Reproduction in domestic animals》2010,45(5):e83-e90

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Characteristics of monoclonal antibodies against Piscirickettsia salmonis   总被引：3，自引：0，他引：3  

A Jamett  J Aguayo  A Miquel  I Muller  R Arriagada  M I Becker  P Valenzuela  & L O Burzio 《Journal of fish diseases》2001,24(4):205-215

A panel of 28 monoclonal antibodies against Piscirickettsia salmonis was produced using a purified fraction of the bacterium. To determine their specificity to the pathogen, the antibodies were assayed by ELISA and indirect immunofluorescence microscopy. Six monoclonal antibodies were selected based on their strong reaction against P. salmonis and absence of cross-reactivity with other common fish pathogens. Western blot analysis showed that the antibodies reacted to several antigens of P. salmonis . Immunofluorescence assays revealed that these antibodies reacted with the same specificity to different isolates of P. salmonis obtained from the south of Chile. This panel of monoclonal antibodies represents an important tool to develop simple, rapid, sensitive and highly specific methods for the detection of the pathogen and diagnosis of the disease.  相似文献   

Nuclear magnetic resonance imaging of the vitreous body   总被引：2，自引：0，他引：2  

R G González  H M Cheng  P Barnett  J Aguayo  B Glaser  B Rosen  C T Burt  T Brady 《Science (New York, N.Y.)》1984,223(4634):399-400

Imaging with proton nuclear magnetic resonance is a valuable new tool for studying the vitreous body of the eye. It is particularly suited for the detection of vitreal liquefaction and intraocular hemorrhage because of the dependence of the signal on the physical environment of water. Conversely, the vitreous body provides a new model for studying changes in proton relaxation times of protein solutions in biological systems.  相似文献   

Multiple effects of urbanization on the biodiversity of developing countries: The case of a fast-growing metropolitan area (Concepción, Chile)   总被引：1，自引：0，他引：1  

Aníbal Pauchard  Mauricio Aguayo  Roberto Urrutia 《Biological conservation》2006,127(3):272-281

Urbanization is increasingly homogenizing the biota of less developed countries. Even though urban sprawl is a worldwide problem, most studies on the effects of urbanization, and the conceptual models have focused on developed countries. South America has not escaped urbanization, and here we discuss the potential impacts of urban sprawl with respect to three ecosystems in the metropolitan area of Concepción, Chile. We consider this area a good model and fairly representative of other cities in developing countries which are also experiencing rapid and uncontrolled growth. We found that the impacts of urban sprawl on biodiversity in the metropolitan area of Concepción differ little from cities in other parts of the world: native ecosystems are replaced by pavements and buildings and what is left of the natural soil is covered with green areas dominated by non-native ornamental species. Wetlands and other peri-urban ecosystems are rapidly being destroyed, fragmented or invaded by non-native species. We found that from a study area of 32,000 ha, there was a net loss to urbanization of 1734 ha of wetlands (23% of the original) and 1417 ha (9%) of agricultural, forest and shrub land cover types between 1975 and 2000. From the total area urbanized (3151 ha), 55% corresponded to wetlands and 45% to agricultural, forest and shrub lands cover types. We see the lack of environmental awareness as a major cause of the increasing deterioration of biodiversity in urban areas of developing countries. More research is needed to fully understand the effects of urban sprawl on the biodiversity of developing countries to include these ecosystems in global conservation strategies.  相似文献   

Pharmacokinetic and pharmacodynamic modelling of intravenous,intramuscular and subcutaneous buprenorphine in conscious cats     

Paulo VM Steagall  Ludovic Pelligand  Tatiana Giordano  Christophe Auberger  John W Sear  Stelio PL Luna  Polly M Taylor 《Veterinary anaesthesia and analgesia》2013,40(1):83-95

ObjectiveTo describe simultaneous pharmacokinetics (PK) and thermal antinociception after intravenous (IV), intramuscular (IM) and subcutaneous (SC) buprenorphine in cats.Study designRandomized, prospective, blinded, three period crossover experiment.AnimalsSix healthy adult cats weighing 4.1 ± 0.5 kg.MethodsBuprenorphine (0.02 mg kg^?1) was administered IV, IM or SC. Thermal threshold (TT) testing and blood collection were conducted simultaneously at baseline and at predetermined time points up to 24 hours after administration. Buprenorphine plasma concentrations were determined by liquid chromatography tandem mass spectrometry. TT was analyzed using anova (p < 0.05). A pharmacokinetic-pharmacodynamic (PK-PD) model of the IV data was described using a model combining biophase equilibration and receptor association-dissociation kinetics.ResultsTT increased above baseline from 15 to 480 minutes and at 30 and 60 minutes after IV and IM administration, respectively (p < 0.05). Maximum increase in TT (mean ± SD) was 9.3 ± 4.9 °C at 60 minutes (IV), 4.6 ± 2.8 °C at 45 minutes (IM) and 1.9 ± 1.9 °C at 60 minutes (SC). TT was significantly higher at 15, 60, 120 and 180 minutes, and at 15, 30, 45, 60 and 120 minutes after IV administration compared to IM and SC, respectively. IV and IM buprenorphine concentration-time data decreased curvilinearly. SC PK could not be modeled due to erratic absorption and disposition. IV buprenorphine disposition was similar to published data. The PK-PD model showed an onset delay mainly attributable to slow biophase equilibration (t_1/2k_e0 = 47.4 minutes) and receptor binding (k_on = 0.011 mL ng^?1 minute^?1). Persistence of thermal antinociception was due to slow receptor dissociation (t_1/2k_off = 18.2 minutes).Conclusions and clinical relevanceIV and IM data followed classical disposition and elimination in most cats. Plasma concentrations after IV administration were associated with antinociceptive effect in a PK-PD model including negative hysteresis. At the doses administered, the IV route should be preferred over the IM and SC routes when buprenorphine is administered to cats.  相似文献   

A statistical model to detect asymptomatic infectious individuals with an application in the Phytophthora alni-induced alder decline     

Elegbede CF  Pierrat JC  Aguayo J  Husson C  Halkett F  Marçais B 《Phytopathology》2010,100(11):1262-1269

In some diseases-in particular, tree root infection-stages of infection and inoculum production level and timing are not readily observable because of uncertainty or time lags in symptom appearance. Here, we pose a criterion, based on relative hazard of disease symptoms, to discriminate between healthy and asymptomatic infected individuals. We design a statistical procedure to estimate the criterion for a 6-year survey of alder decline along a northeastern French river. Individual tree symptom hazard was modeled with Cox's regression model, taking estimation of local infection pressure as a risk factor. From an inoculum production experiment, we thereafter assessed the inoculum production level of target trees, including symptomatic and asymptomatic trees ranked according to their symptoms hazard. Using receiver operating characteristic methods, we first evaluated the criterion performance and determined the discrimination threshold to sort out asymptomatic individuals into healthy and infected. Then, we highlighted the fact that the infected asymptomatic trees were among the major inoculum producers whereas severely declining and dead trees were found to be poor inoculum sources.  相似文献   

Postoperative analgesic effects of intravenous,intramuscular, subcutaneous or oral transmucosal buprenorphine administered to cats undergoing ovariohysterectomy     

Tatiana Giordano  Paulo VM Steagall  Tatiana H Ferreira  Bruno W Minto  Sílvia Elaine Rodolfo De Sá Lorena  Juliana Brondani  Stelio PL Luna 《Veterinary anaesthesia and analgesia》2010,37(4):357-366

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Assessment of molecular detection of Fusarium circinatum in insects and passive spore traps in Pinus radiata plantations     

Jaime Aguayo  Cline Fourrier‐Jeandel  Xavier Capdevielle  Fabrice Vtillard  Dominique Piou  Eugenia Iturritxa  Ccile Robin 《Forest Pathology》2020,50(1)

Fusarium circinatum is the causal agent of pitch canker, a destructive disease that threatens natural and planted pine forests around the world. Although pitch canker has caused problems in Spain and Portugal, concerning Europe as a whole, the fungus is not established across the pine distribution area. Its dispersion by wind and/or insect vectors could nevertheless play a role in the colonization of currently uninfected stands. It is therefore crucial to develop monitoring tools for its detection. To this end, we assessed the molecular detection of the pathogen in environmental samples of bark beetles and passive spore traps, collected in two infected Pinus radiata plantations in Basque country, Spain. The spread pattern of F. circinatum was assessed by an experimental design that included insect and spore traps installed at the centre, at the edge and outside the plots. Our results showed that F. circinatum was detected in both types of samples, at almost all collection dates. In both type of samples, positive detections were mainly found at the centre of the plots, a lower proportion at the edge, and very few outside. This suggests that long‐distance dispersion of Fusarium circinatum does not rely on wind spore dispersal neither on insect flight. Our study also shows that molecular methods are a powerful tool to monitor the pathogen in environmental samples.  相似文献   

Salmon cells SHK‐1 internalize infectious pancreatic necrosis virus by macropinocytosis     

Jorge Levicn‐Asenjo  Ricardo Soto‐Rifo  Francisco Aguayo  Aldo Gaggero  Oscar Leon 《Journal of fish diseases》2019,42(7):1035-1046

We have previously shown that infectious pancreatic necrosis virus (IPNV) enters the embryo cell line CHSE‐214 by macropinocytosis. In this study, we have extended our investigation into SHK‐1 cells, a macrophage‐like cell line derived from the head kidney of Atlantic salmon, the most economically important host of IPNV. We show that IPNV infection stimulated fluid uptake in SHK‐1 cells above the constitutive macropinocytosis level. In addition, upon infection of SHK‐1 cells, IPNV produced several changes in actin dynamics, such as protrusions and ruffles, which are important features of macropinocytosis. We also observed that the Na+/H+ pump inhibitor EIPA blocked IPNV infection. On the other hand, IPNV entry was independent of clathrin, a possibility that could not be ruled out in CHSE 214 cells. In order to determine the possible role of accessory factors on the macropinocytic process, we tested several inhibitors that affect components of transduction pathways. While pharmacological intervention of PKI3, PAK‐1 and Rac1 did not affect IPNV infection, inhibition of Ras and Rho GTPases as well as Cdc42 resulted in a partial decrease in IPNV infection. Further studies will be required to determine the signalling pathway involved in the macropinocytosis‐mediated entry of IPNV into its target cells.  相似文献