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1.
Seyyed Aliakbar Sheikholeslami Ali Soleimanzadeh Alaleh Rakhshanpour Dariush Shirani 《Reproduction in domestic animals》2020,55(9):1229-1239
The aim of this study was to evaluate the effects of different concentrations of lycopene and cysteamine on characteristics of sperm, liquid peroxidation and enzymatic activities in seminal plasma of canine semen preserved at 5°C for 72 hr. The semen samples were divided into eight aliquots: control, control sham (dimethyl sulfoxide 5%), lycopene groups (250, 500 and 750 µg/ml) and cysteamine groups (2.5, 5 and 10 mM). Motility, viability, membrane integrity, DNA integrity, total antioxidant capacity (TAC) and malondialdehyde (MDA) levels were evaluated. Progressive motility and total motility were higher with the 500 and 750 µg/ml lycopene concentrations, respectively, compared to the control group and the cysteamine groups following 72 hr of storage in the liquid storage. Motility characteristics, viability and hypo-osmotic swelling test (HOST) percentages were significantly improved in 500 µg/ml lycopene compared to other groups. The 500 and 750 µg/ml lycopene concentrations, respectively, showed significantly reduced percentages of spermatozoa with DNA integrity compared to the control group. The 500 and 750 µg/ml lycopene concentrations, respectively, led to the significant decrease of MDA levels. The 500 µg/ml lycopene enhanced TAC levels after 48 and 72 hr that was not observed in other groups. In conclusion, the findings of this study showed that lycopene supplementation in canine semen extenders improved canine semen parameters and TAC levels and decreased MDA levels in the chilling process. 相似文献
2.
Rhizosphere effects on Cu availability and fractionation in sewage sludge‐amended calcareous soils 
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下载免费PDF全文 Rhizosphere processes have a major impact on copper (Cu) availability and its fractions in soils. A greenhouse experiment with wheat was performed to investigate availability (using seven chemical procedures) and fractionation of Cu in the rhizosphere of ten agricultural soils (Typic Calcixerepts) amended with sewage sludge (1% w/w) using rhizoboxes. The results show that available Cu concentrations in rhizosphere soils were significantly (P < 1%) lower than in bulk soils. In comparison with the bulk soils, in the rhizosphere soils the concentration of Cu associated with organic matter and residual Cu increased by 24 and 4%, respectively, whereas exchangeable Cu, Cu associated with iron‐manganese oxides, and Cu associated with carbonate decreased by 20, 14, and 12%, respectively. Dissolved organic carbon (DOC) and Cu associated with iron‐manganese oxides and Cu associated with organic matter in the rhizosphere and bulk soils were significantly correlated (P < 5%). The results show that the differences between rhizosphere and bulk soils in chemical conditions such as DOC concentrations can change the proportion of soil Cu fractions and, therefore, Cu availability for wheat in calcareous soils amended with sewage sludge. The results show that the wheat root‐induced modifications of chemical and biological soil conditions do not only lead to Cu depletion in mobile soil Cu fractions, but also to modification in soil Cu fractions which are commonly considered as more stable. 相似文献
3.
A greenhouse experiment was carried out to determine copper (Cu) desorption characteristics in ten bulk and rhizosphere soils (Typic Calcixerepts) amended with sewage sludge (1% w/w) using rhizoboxes. The kinetics of Cu desorption in the bulk and the rhizosphere soils were determined after successive extraction with DTPA‐TEA and 10 mM citric acid in a period of 1 to 504 h at (25 ± 1)°C. The results show that Cu extracted after 504 h using DTPA‐TEA were significantly (P < 1%) lower in the rhizosphere than the bulk soils. However, Cu extracted after 504 h using citric was significantly (P < 1%) higher in the rhizosphere than in the bulk soils. The results illustrated that, on average, citric acid extracted 56% more Cu from the bulk soils than DTPA‐TEA, and citric acid extracted 85% more Cu from the rhizosphere soils than DTPA‐TEA. Desorption kinetics of Cu in the two extractants was well described by power‐function, parabolic diffusion, and first‐order equations. The results show that a 10 mM citric acid extractant may be recommended to determine the kinetics of Cu desorption in calcareous soils amended with sewage sludge. 相似文献
4.
Akram Farshadirad Ali Reza Hosseinpur Hamid Reza Motaghian Shoja Ghorbani Dashtaki 《Archives of Agronomy and Soil Science》2017,63(10):1419-1430
Zinc (Zn) distribution in different soil aggregates can affect Zn availability. In this study, the effect of soil aggregate-size fractions on Zn distribution and availability was determined in some heavy metal-contaminated soils. Air-dried samples were fractionated into four different aggregate-size fractions (2.0–4.0, 0.25–2.0, 0.05–0.25 and <0.05 mm). Extraction efficiency of available Zn determined by using Mehlich-3, DTPA-TEA, Mehlich-1 and H2O methods in different aggregates and relation between extracted Zn and corn indices were studied. Moreover, the Tessier fractionation scheme was applied to determine the partitioning of Zn in different aggregates. Among all the extractants, Mehlich-3 showed better extractability of Zn from soils and the highest amount of extracted Zn was found in the <0.05 (87.65 mg kg?1) and 0.05–0.25 (80.86 mg kg?1) mm fractions. Zn extracted by Mehlich-3 and DTPA-TEA in the <0.05 and 0.05–0.25 mm fractions had significant correlation with the amount of Zn in corn. Also, correlation coefficients between carbonate-bound and Fe–Mn oxide-bound Zn and available Zn in <0.05 and 0.05–0.25 mm aggregates was higher than other aggregates, suggesting that this two fraction of Zn in finer aggregates constituted the major available Zn pools in the studied soils. 相似文献
5.
Mottaghian A Pirdashti H Bahmanyar MA Abbasian A 《Pakistan journal of biological sciences: PJBS》2008,11(9):1227-1233
Plant nutrients can be influenced by organic materials of soils. An experiment was conducted to evaluate the effect of organic amendments on elements uptake by soybean cultivars in a silty loam soil in Mazandaran province, Iran. The experiment was carried out in split plot based on randomized complete block design with three replications in 2006. Main plots were included 8 fertilizer treatments consisted of 20 and 40 Mg ha(-1) Municipal Solid Waste Compost (MSW), Vermicompost (VC) and Sewage Sludge (SS) which enriched with 50% chemical fertilizers needed by soil, only chemical fertilizer treatment and control. Sub plots consisted of three genotypes of soybean (032, 033 and JK). Grain yield was determined and soybean leaves and seeds were digested and analyzed for Mn, Cu, Zn and Fe. Results showed that yield and elements content in soybean leaves and seeds (Mn, Cu, Zn and Fe) were influenced by all treatments. The 40 Mg ha(-1) of sewage sludge enriched with chemical fertilizers produced maximum grain yield. Different soybean cultivars had also significant differences in terms of leaf and seed micronutrients accumulation. Maximum grain yield was observed in JK and 033. Mean comparisons showed that interaction effects of fertilizer and cultivar had significant differences on Mn, Cu and Fe content in soybean leaves, so that the maximum Cu content was observed in 032 cultivars with 40 Mg ha(-1) enriched sewage sludge and municipal waste compost. Also the highest amount of Fe was obtained for JK cultivar when the 40 Mg ha(-1) of municipal compost was used. Among different mentioned traits, Fe and Cu content in leaf and seed and Zn content in leaf had a positive and significant correlation with grain yield. 相似文献
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7.
Nastaran Shakouri Ali Soleimanzadeh Alaleh Rakhshanpour Mustafa Numan Bucak 《Reproduction in domestic animals》2021,56(7):1004-1014
Supplementing the extender with antioxidants with low molecular weight can enhance the quality of the post-thaw sperm during the freezing process. This study was aimed at determining the impacts of 3,4-dihydroxyphenyl glycol (DHPG) on the spermatozoa of the canine undergoing freeze-thawing process. In this study, 24 ejaculates were obtained from three mixed-breed dogs and were diluted in a Tris-based extender. The diluted semen was divided into aliquots for supplementation of 10, 30, 50 and 70 µg/ml of DHPG, control (without antioxidant) and control sham (DMSO). After being extended, the semen was equilibrated at a temperature of 4°C and then transferred to the straws and kept 4 cm above the liquid nitrogen for 20 min and was finally immersed in the liquid nitrogen. They were cryopreserved for seven days; then, sperm parameters including sperm motility evaluation, motility characteristics, viability, DNA and plasma membrane integrity, total antioxidant capacity (TAC), reduced glutathione content (GSH), antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT] and glutathione peroxidase [GPx]) activity malondialdehyde (MDA) levels were evaluated. This study showed that spermatozoa cryopreservation with 50, 30 and 70 µg/ml of DHPG concentrations had better progressive motility, Curvilinear Velocity, Linearity, viability, intact plasma membrane and the levels of TAC, GPx and GSH were higher than the control group. The 50, 30 and 70 µg/ml of DHPG concentrations led to the significant decrease of DNA damage compared to the control group. Total motility, average path velocity, straight-line velocity and CAT activity were significantly improved in 30 and 50 µg/ml of DHPG concentrations, compared to the control group. Also, the 50 and 30 µg/ml of DHPG concentrations, decreased MDA levels compared to the other groups, significantly. In conclusion, our study showed that the addition 50 µg/ml of DHPG to the canine semen extender improved the semen characteristics and oxidative markers in the cryopreservation process. 相似文献
8.
Mani A Radhakrishnan J Wang H Mani A Mani MA Nelson-Williams C Carew KS Mane S Najmabadi H Wu D Lifton RP 《Science (New York, N.Y.)》2007,315(5816):1278-1282
Coronary artery disease (CAD) is the leading cause of death worldwide and is commonly caused by a constellation of risk factors called the metabolic syndrome. We characterized a family with autosomal dominant early CAD, features of the metabolic syndrome (hyperlipidemia, hypertension, and diabetes), and osteoporosis. These traits showed genetic linkage to a short segment of chromosome 12p, in which we identified a missense mutation in LRP6, which encodes a co-receptor in the Wnt signaling pathway. The mutation, which substitutes cysteine for arginine at a highly conserved residue of an epidermal growth factor-like domain, impairs Wnt signaling in vitro. These results link a single gene defect in Wnt signaling to CAD and multiple cardiovascular risk factors. 相似文献
9.
Hemmatollah Pirdashti Alaleh Motaghian Mohammad Ali Bahmanyar 《Journal of plant nutrition》2013,36(4):485-495
A field experiment was conducted to evaluate the effect of organic amendments on grain yield, leaf chlorophyll content, and some morphological characteristics of three soybean cultivars in Mazandaran province located at north of Iran in 2006. Chemical fertilizer (75 kg ha?1 potassium sulfate and triple superphosphate), two levels of municipal solid waste, vermicompost and sewage sludge biosolid (20 and 40 Mg ha?1) enriched with%50 chemical fertilizers needed by soil were applied to soybean cultivars (‘032’ and ‘033’ promising lines and ‘JK’ cultivar). The experiment was carried out in split plot based on randomized complete block design with three replications. Some important plant characters such as grain yield, leaf chlorophyll content, number of branches, number of nodes on the main stem, length of internodes, stem diameter, first pod height and plant height were determined. Results showed that application of 40 Mg ha?1 sewage sludge enriched with chemical fertilizers increased plant grain yield and stem diameter and application of 40 Mg ha?1 vermicompost enriched with half chemical fertilizer increased the number of nodes on the main stem, significantly. The maximum length of internodes, first pod height and plant height were obtained when the 40 Mg ha?1 sewage sludge and vermicompost enriched with half chemical fertilizer in ‘032’ line was used. Biomass, number of branches, stem diameter, number of nodes on the main stem of soybean cultivars had a positive and significant correlation with grain yield. 相似文献
10.
Hamid Reza Motaghian Ali Reza Hosseinpur 《Communications in Soil Science and Plant Analysis》2017,48(18):2126-2136
The application of organic fertilizers in soils not only increases soil organic matter but also introduces essential nutrients to soil. Therefore, applying these fertilizers can affect the availability and desorption characteristics of nutrients. The main objective of this research is to study the effects of cow manure (CM) and vermicompost (VC) on availability and desorption characteristics of zinc (Zn) in a loamy calcareous soil. In this study, concentration of available Zn (using DTPA-TEA, AB-DTPA, and Mehlich 3) and desorption characteristics of Zn (using successive extraction with DTPA-TEA For 1–504 h at 25 ± 1°C) in amended soil with 0, 0.5, and 1% (w/w) of CM and VC were investigated in a completely randomized design. Results of this research showed that concentration of Zn extracted by using three methods was higher in amended soils with 1% CM and VC than those with 0.5% of these fertilizers. Furthermore, the difference between concentration of available Zn in amended soils with CM and VC was not found to be significant (p > 0.05). The results of kinetics study illustrated that the effect of organic fertilizers on Zn desorbed after 504 h was found to be significant (p < 0.01). Amount of cumulative of Zn desorbed in amended soils was significantly (p < 0.05) higher than unamended soil. Concentration of Zn desorbed after 504 h in 0.5 and 1% of CM and VC compared with unamended soil increased 26, 54, 12, and 46%, respectively. In addition, Zn desorption rate in amended soils with CM was higher than those with VC. It can be concluded that organic fertilizers applied to loamy calcareous soils enhance source of available Zn for the plant. Moreover, the results of this study showed that the ability of amended soils with VC to supplying Zn for plants was lower than those with CM. 相似文献