Differentiation of Staphylococcus sciuri Strains Isolated from Free‐Living Rodents and Insectivores     

T. Hauschild  S. Schwarz 《Zoonoses and public health》2003,50(5):241-246

Twenty‐nine Staphylococcus sciuri strains isolated from free‐living insectivores and rodents were comparatively analysed for their biochemical capacities and their SmaI macrorestriction patterns. The 29 S. sciuri isolates represented 21 different biotypes and 22 different SmaI macrorestriction types. This observation confirmed that S. sciuri isolates obtained from insectivores and rodents living in natural environments constituted a heterogeneous population. Cluster analysis revealed that the macrorestriction patterns and the biochemical profiles matched in some cases. However, S. sciuri isolates that exhibited the same or closely related biochemical profiles were also found to be associated with different macrorestriction patterns. Analysis of the 29 S. sciuri isolates for their plasmid content and their sensitivity to antimicrobial agents showed that most of the isolates were plasmid‐free and susceptible to all antimicrobial agents tested.  相似文献   

Plasmids and resistance to antimicrobial agents and heavy metals in Staphylococcus hyicus from pigs and cattle   总被引：1，自引：0，他引：1  

S Schwarz  H Blobel 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1989,36(9):669-673

Staphylococcus hyicus-cultures, isolated from piglets and cattle with skin lesions were investigated for their plasmid content and their resistance to antimicrobial agents and heavy metals. Several plasmids of different sizes could be detected in most of the 32 "porcine" S. hyicus-isolates, whereas none of the 20 "bovine" S. hyicus-cultures possessed any plasmid. The "porcine" S. hyicus-isolates were much more resistant to antimicrobial substances than the "bovine" S. hyicus-cultures. However, the "porcine" and "bovine" S. hyicus-cultures did not differ in their resistance to heavy metals.  相似文献   

Detection of a novel chloramphenicol resistance plasmid from "equine" Staphylococcus sciuri   总被引：3，自引：0，他引：3  

S Schwarz  M Cardoso  H Blobel 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1990,37(9):674-679

A small chloramphenicol resistance (Cm) plasmid of 4.65 kB could be detected in an "equine" Staphylococcus sciuri-culture. This plasmid, designated as pSC3, was identified by interspecific protoplast transformation. On the basis of restriction endonuclease analyses a detailed restriction map of pSC3 could be constructed. This allowed structural comparisons of pSC3 with Cm-plasmids of other staphylococcal species from infections of humans and animals and identification of pSC3 as a member of the pC 221-family of staphylococcal Cm-plasmids. The pSC3-plasmid encoded an inducible chloramphenicol acetyltransferase as confirmed by enzymatic assays. This enzyme could be demonstrated in cell-free lysates of Cm-induced pSC3-transformants.  相似文献   

Plasmid-encoded antibiotic resistance in Staphylococcus hyicus     

S Schwarz  M Cardoso  H Blobel 《DTW. Deutsche tier?rztliche Wochenschrift》1990,97(11):498, 501-498, 503

A total of 33 Staphylococcus hyicus-cultures from piglets with exudative epidermatitis were analyzed for the presence of antibiotic resistance plasmids. Four small plasmids encoding resistances to chloramphenicol, macrolide-lincosamide-antibiotics, streptomycin or tetracyclines could be identified in plasmid-curing and plasmid-transformation experiments. For further characterization these plasmids were digested with restriction endonucleases. This led to the construction of a specific restriction map for each of the 4 plasmids. On the basis of their restriction maps, these 4 antibiotic resistance plasmids from "porcine" S. hyicus-cultures were compared with the respective resistance plasmids of other staphylococcal species from infections of humans and animals.  相似文献   

Beschreibung des Feuerbrandbefalls am Institut für Obstzüchtung in Dresden-Pillnitz im Jahr 2003     

Andreas Peil  Klaus Richter  Monika Höfer  Viola Hanke 《Erwerbs-Obstbau》2004,46(6):141-148

The orchard of the Institute of Fruit Breeding of the German Federal Centre of Breeding Research on Cultivated Plants in Dresden-Pillnitz was highly affected by fire blight in 2003. Infected pomefruit trees were observed over a period of nearly 3 months. The first symptoms on pear trees were found on May 19th. The pathogen Erwinia amylovora was confirmed officially on May 26, and the last infected apple trees were detected the 11th of August. The infected trees had to be grubbed at the decision of the Phytopathological Authority. In total, 1164 apple and 478 pear trees were grubbed, including the entire pear collection of the gene bank. Of 35 wild species of pear, 49 accessions, eight accessions of six species each, showed infections. The apple collection of the gene bank included 33 wild species, with 365 accessions, and 845 cultivars and clones. Ten accessions of nine wild apple species and 81 cultivars/clones of these collections showed fire blight infection. The source of infection was the pear collection, and the distance from that source was important for the occurrence of infection. Field plots close to the pear collection had tree losses of 10–34%, while more distant plots had losses of 0–6%. Around 80% of the lost apple trees were detected and grubbed from 27th May to 11th June. Some of the cultivars bred in Dresden-Pillnitz, e.g. ‘Pilot’ and ‘Rekarda’, were affected by fire blight in most field plots, whereas most others were affected mainly only in plots adjacent to the infection source. A correlation of r=?0.72 could be calculated for rating in artificial shoot inoculations and percentage of trees of resistant cultivars lost. The cultivars ‘Pirol’, ‘Reanda’, ‘Remo’, ‘Rene’, ‘Renora’, ‘Resi’, and ‘Retina’ showed only a very low numbers of infected trees. No tree of ‘Rewena’ showed symptoms of fire blight. Despite a tendency to postblooming, only 8.9% of ‘Pinova’ trees had to be grubbed.  相似文献   

Evaluating the measuring software package Metron-PX for morphometric description of equine hoof radiographs     

Jairo Vargas Rocha DVM  Christophorus J. Lischer DVM Habil  ECVS  Martin Kummer DVM  Michael Hssig DVM Habil  MPH  FVH  ECBHM  Jrg Andreas Auer DVM Prof  ACVS  ECVS 《Journal of Equine Veterinary Science》2004,24(8):347-354

  相似文献   

Computing inbreeding coefficients and the inverse numerator relationship matrix in large populations of honey bees          下载免费PDF全文

Richard Bernstein  Manuel Plate  Andreas Hoppe  Kaspar Bienefeld 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2018,135(4):323-332

The inbreeding coefficients are considered in breeding decisions, and the inverse numerator relationship matrix A ^?1 is a prerequisite for breeding value estimation. Polyandry and haploid males are among the specifics of relationships between honey bees. Brascamp and Bijma (2014) averaged out the manifold possible relationships among honey bees that appear to have the same parents in a pedigree and assigned a single entry in A to animals that behave as a unit, for example, the workers of a hive. Their methods of calculation connected full‐sibs in the variance matrix of the Mendelian sampling terms D , via nonzero off‐diagonal elements. This impedes the inversion of A and the closely connected calculation of inbreeding coefficients, because efficient algorithms for this task take D to be a diagonal matrix. Memory limitations necessitate their use for large data sets. We adapted the quickest of them to the block diagonal matrix D , that is postulated for the honey bee. To our knowledge, the presented algorithm is the first one that facilitates the method of Brascamp and Bijma (2014) on large data sets.  相似文献   

Randomized controlled clinical study evaluating the efficacy and safety of intratumoral treatment of canine mast cell tumors with tigilanol tiglate (EBC‐46)     

Thomas R. De Ridder  Justine E. Campbell  Cheryl Burke‐Schwarz  David Clegg  Emily L. Elliot  Samuel Geller  Wendy Kozak  Stephen T. Pittenger  Jennifer B. Pruitt  Jocelyn Riehl  Julie White  Melissa L. Wiest  Chad M. Johannes  John Morton  Pamela D. Jones  Peter F. Schmidt  Victoria Gordon  Paul Reddell 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):415-429

  相似文献   

Antimicrobial susceptibility of Escherichia coli from swine, horses, dogs and cats as determined in the BfT-GermVet monitoring program 2004-2006     

Grobbel M  Lübke-Becker A  Alesík E  Schwarz S  Wallmann J  Werckenthin C  Wieler LH 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(9-10):391-401

A total of 417 isolates of Escherichia coli collected from five animal species/organ system combinations from swine [urinary/genital tract (UGT) incl. mastitis metritis agalactia syndrome], horses [genital tract (GT)] and dogs/cats [respiratory tract (RT), UGT and gastrointestinal tract (GIT)] were analysed quantitatively for their susceptibility against different antimicrobial agents by determination of minimum inhibitory concentrations. Regardless of which animal species the strains originated from, resistance appeared most frequently against sulfamethoxazole (18-59%), tetracycline (14-54 %), and ampicillin (14-39%). High percentages of intermediate isolates were observed for cephalothin (39-46 %). In general, low prevalences of resistance were detected for amoxicillin/clavulanic acid (1-4%), gentamicin (1-9%), and cefazolin (0-11%). Generally speaking, the antimicrobial resistance situation among E. coli isolates from horses and small animals is relatively good.  相似文献   

Antimicrobial susceptibility of Pasteurella multocida and Bordetella bronchiseptica from dogs and cats as determined in the BfT-GermVet monitoring program 2004-2006     

Schwarz S  Alesík E  Grobbel M  Lübke-Becker A  Werckenthin C  Wieler LH  Wallmann J 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(9-10):423-430

A total of 92 canine/feline Pasteurella multocida strains form respiratory tract infections or infections of skin/ear/mouth as well as 42 canine/feline Bordetella bronchiseptica strains from respiratory tract infections were investigated for their susceptibility to antimicrobial agents. While the P. multocida strains were susceptible to all antimicrobial agents tested - except sulfonamides -, a considerable number of the B. bronchiseptica strains was resistant or exhibited high MIC values against a number of antimicrobial agents including penicillin G, oxacillin, cefazolin, ceftiofur, cefquinome, sulfamethoxazole, and trimethoprim/sulfamethoxazole.  相似文献