Apparent Digestion Coefficients and Metabolizable Energy of Feed Ingredients for Rainbow Trout Oncorhynchus mykiss   总被引：1，自引：0，他引：1  

Robert R.  Smith Robert A.  Winfree Gary W.  Rumsey Anila  Allred Montie  Peterson 《Journal of the World Aquaculture Society》1995,26(4):432-437

More than 50 feedstuffs used or of potential use in diets for rainbow trout Oncorhyndus mykiss were compared through metabolism chamber trials. Testing included determination of apparent digestibility and metabolic energy for a wide variety of ingredients, including fish and animal meals, vegetable proteins, and single cell protein products. Testing involved feeding individual 200–300-g fish contained in replicate chambers, from which feces, urine, and gill excreta were collected seg arately and analyzed for residual nitrogen and energy. For comparison, several products previously tested at this or other laboratories were re-evaluated at dietary concentrations of 25, 50, or 100%.  相似文献   

A Single-Tube,Functional Marker-Based Multiplex PCR Assay for Simultaneous Detection of Major Bacterial Blight Resistance Genes Xa21,xa13 and xa5 in Rice     

K. Hajira S.  M. Sundaram R.  S. Laha G.  Yugander A.  M. Balachandran S.  C. Viraktamath B.  Sujatha K.  H. Balachiranjeevi C.  Pranathi K.  Anila M.  Bhaskar S.  Abhilash V.  K. Mahadevaswamy H.  Kousik M.  Dilip Kumar T.  Harika G.  Rekha G. 《水稻科学》2016,23(3):144

In marker-assisted breeding for bacterial blight(BB) resistance in rice, three major resistance genes, viz., Xa21, xa13 and xa5, are routinely deployed either singly or in combinations. As efficient and functional markers are yet to be developed for xa13 and xa5, we have developed simple PCR-based functional markers for both the genes. For xa13, we designed a functional PCR-based marker, xa13-prom targeting the In Del polymorphism in the promoter of candidate gene Os8N3 located on chromosome 8 of rice. With respect to xa5, a multiplex-PCR based functional marker system, named xa5 FM, consisting of two sets of primer pairs targeting the 2-bp functional nucleotide polymorphism in the exon II of the gene TFIIA5(candidate for xa5), has been developed. Both xa13-prom and xa5 FM can differentiate the resistant and susceptible alleles for xa13 and xa5, respectively, in a co-dominant fashion. Using these two functional markers along with the already reported functional PCR-based marker for Xa21(p TA248), we designed a single-tube multiplex PCR based assay for simultaneous detection of all the three major resistance genes and demonstrated the utility of the multiplex marker system in a segregating population.  相似文献   

Factors influencing crossability among triticale and wheat and its subsequent effect along with hybrid necrosis on haploid induction     

Navdeep S. Jamwal  H. K. Chaudhary  Anila Badiyal  Waseem Hussain 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2016,66(3):282-289

Gene introgression in wheat is not only limited by crossability but also affected by environmental conditions, genotypic differences and necrotic genes. Hence, the present investigation was formulated to determine the influence of genotypic and environmental factors on the crossability of elite triticale and wheat genotypes, screen the effect of necrotic genes in the F₁ hybrids and the relative performance of hybrids towards haploid induction following the Imperata cylindrica-mediated chromosome elimination approach. The dry temperate region was observed to be significantly better than sub-humid mid-hills for seed set in intergeneric crosses. Lower rate of crossability (17.49–48.33% and 14.46–36.51%) was found in triticale?×?wheat crosses at both locations. The hybrids having HPW236 as one of the parent revealed necrosis ranging from the lowest to highest grade. Further, negative influence of the necrotic genes on the haploid induction parameters was apparent due to significant hybrid necrosis symptoms in F₁ hybrids. Statistically significant variation in haploid induction parameters was also evident between and within different cross combinations, indicating that haploid induction parameters were profoundly influenced by genotypic specificity.  相似文献   

Biodiversity in wood-decay macro-fungi associated with declining arid zone trees of India as revealed by nuclear rDNA analysis     

S. K. Singh  Anila Doshi  Anjly Pancholy  Rakesh Pathak 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(2):373-382

Wood-decay macro-fungi have been reported to be a major cause of decline of arid zone trees in the genera Acacia, Prosopis, Ziziphus and Azadirachta; the identity of these fungi, however, has not been confirmed in the state of Rajasthan in northwestern India. Direct sequencing of PCR amplified ITS regions of rDNA facilitated molecular identification of 26 isolates into species in eight genera (Ganoderma, Inonotus, Phellinus, Ceriporia, Schizophyllum, Phanerochaete, Pleurotus, Leucoagaricus) and one incompletely characterized Basidiomycota species. In addition to intra-specific phenotypic variations in basidiocarp morphology, Single Nucleotide Polymorphisms (SNPs) were recorded among isolates of P. pulmonarius, S. commune, G. lucidum and Ganoderma species. Both SNPs and insertions/deletions (INDELS) were recorded amongst Inonotus species. A phylogram based on multiple sequence alignment delineated all isolates into separate intra-specific subgroups along with their GenBank reference sequences with high bootstrap values. Phellinus repandus, L. meleagris, C. lacerata and three Inonotus species (I. rickii, I. patouillardii, and I. porrectus) reported herein are new fungal records from India.  相似文献