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K. Aranka Deér János Nemcsók LászIó Boross Béla Szajáni 《Fish physiology and biochemistry》1990,8(1):79-83
The effects of paraquat (PQ; 1,1′-dimethyl-4,4′-bipyridylium dichloride) treatment were investigated in carp, silver carp
and wels. The serum glutamic-oxalacetic transaminase (GOT; L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1.) level
was enhanced by 50% at 1 ppm exposure and by 100% at 10 ppm exposure in all species, and there was a change in the distribution
of the molecular subforms of GOT in the liver and heart. The activities of the individual subforms decreased with increasing
PQ concentration or after a longer exposure. In some cases, one of the subforms was no longer present in the liver. An increased
serum GOT activity, a decreased enzyme activity in different organs and the disappearance of molecular subform indicate tissue
damage. 相似文献
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The kinetics of cadmium uptake by Selenastrum capricornutum Printz was studied at 21 °C and 0 °C and at four different concentrations of free cadmium (10?9, 10?8, 10?7 and 10?3 M). The free cadmium concentrations in the medium were kept constant through buffering by a chelating agent (NTA). Initial uptake occurred within 10 min at both temperatures, indicating a fast process. At 21 °C, the cadmium uptake increased during the experimental sampling times from 0.12 to 50 h, whereas at 0 °C, the cadmium uptake remained constant after the first sampling time. Apparently, two different processes were involved in cadmium uptake, a first fast, probably passive process and a second slower, possibly physiological process. The cadmium uptake processes are described with a generalized metalligand complex formation model. The experimental dissociation constants for the fast (K R,F ) and the slow process (K R,S ) were reproducible for different experiments and they were both in the order of 5 10?6 mol L?1. For the slow process, the uptake- and release rate constants (k1 and k2) were quantified; for two experiments, the mean value for ki was 111±28 L mol?1 h?1 and the mean value for k 2 was (5.1±1.8) 10?4 h?1. These values were calculated using the fitted value of the cadmium uptake in the fast process. 相似文献
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