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This study evaluated the effect of supplement of raw potato starch (RPS) on the levels of skatole, androstenone, testosterone and oestrone sulphate in plasma from entire male pigs. The study also evaluated relationships between plasma levels of skatole and testicular steroids at three different live weights (LW) of approximately 90, 100 and 115 kg. A total of 111 entire male pigs of a crossbred (Yorkshire dams×Swedish Landrace sires) were used. Animals were raised either in mixed pens, with females and males, or single-sex pens. Each pen contained seven or nine pigs. The most fast-growing three pigs from the pens with nine pigs were slaughtered when they reached 90 kg LW, and the remaining pigs were slaughtered at 115 kg LW. All pigs were fed the same commercial diet until the average pen weight reached 100 kg. Then, 33 out of 80 remaining pigs received RPS, 0.6 kg per pig and day, for 2 weeks prior to slaughter. Blood samples were taken from the pigs at three occasions: first, the day prior to first slaughter occasion, low-weight group; second, the day prior to change in diet, middle-weight group; and third, the day prior to second slaughter occasion, high-weight group. Plasma was analysed for the levels of skatole, androstenone, testosterone and oestrone sulphate. Fat samples were taken at slaughter and analysed for the levels of skatole and androstenone. The levels of skatole and testicular steroids in plasma were significantly higher in entire male pigs from the high-weight group fed no RPS compared to those from low- and middle-weight groups. The levels of the investigated compounds did not differ between low- and middle-weight groups (P>0.1). The diet with RPS induced a decline in skatole levels in plasma and fat (P<0.001), but not plasma levels of testicular steroids and fat levels of androstenone (P>0.05). Skatole levels were positively correlated to testosterone and oestrone sulphate levels in the middle- and high-weight pigs fed no RPS as well as to testosterone in the low-weight group. In the high-weight group fed RPS, skatole levels were not correlated to any of the analysed compounds. Approximately 26% of the entire male pigs (11 out of 43) from the high-weight group fed no RPS produced skatole levels in fat above 0.20 μg/g, whereas the pigs from the low- and high-weight group fed RPS did not produce skatole levels above 0.20 μg/g in fat. Androstenone levels in fat were high in all groups. In total 47% (52 out of 111) pigs expressed androstenone levels above the rejection levels in fat of 1.0 μg/g and 88% (98 out of 111) had androstenone levels above 0.5 μg/g. It was concluded that a lower slaughter weight and the supplement of raw potato starch to the diet could be used to reduce skatole levels in entire male pigs. Androstenone levels in fat, however, could not be reduced by either a lower weight at slaughter or dietary manipulation. 相似文献
3.
Since its detection in 2001 the human Metapneumovirus (HMPV), a member of the Paramyxoviridae family, was observed to be a serious pathogen in human respiratory infections during childhood. Meanwhile, several animal models have been established to study the virus-host interactions and pathogenic effects. Mainly, small laboratory animals like mice and cotton rats have been used, although the usage of these two species for HMPV research is controversially discussed and contradictious results were obtained by different groups. Further trials with ferrets, hamsters and non human primates were performed revealing different success in their individual usage. In this review we present the different animal models, summarize their advantages and disadvantages, and discuss the controversial results from different studies. 相似文献
4.
Recruitment of sympatric vendace (Coregonus albula) and whitefish (C. lavaretus) is affected by different environmental factors 下载免费PDF全文
Vendace and whitefish in Lake Osensjøen (boreal south‐east Norway) were studied by means of gillnet test fishing (1976–2013) and hydro acoustic acquisition (1986–2011). Vendace increased in number between 1998 and 2009 while growth and size at maturity decreased. The relative density of whitefish decreased in the pelagic habitat, whereas growth and size remained the same. Both species exhibited varying year‐class strength. Whereas strong year‐classes of both species became less frequent after 1980 than before this, this seemed to change after 2000, especially for vendace. Generalised additive models suggested a strong positive relationship between July/August mean air temperature and year‐class strength of both vendace and whitefish. Whitefish recruitment was also negatively affected by the new regulation regime implemented since 1981, and positively correlated with water level after hatching and by late ice off. The reason for the diverging impacts of environmental factors on the two species may be that vendace spawn in deeper waters that whitefish do. The results indicate that increasing summer temperatures benefit recruitment of both species, whereas low water level and early ice off will harm whitefish recruitment. Both trends are caused by climate warming. It may be speculated that increased density of the specialised plankton feeding vendace may affect the algal community through increased predation on herbivorous zooplankton and potentially affect the trophic state of the lake. 相似文献
5.
OAT Peltoniemi A Tast JV Virolainen V Karkamo M Heinonen MA Andersson 《Reproduction in domestic animals》2005,40(3):224-227
This study was conducted to determine whether the seasonal delay in puberty in autumn is driven by individual differences in night-time melatonin secretion in domestic gilts at the attainment of puberty. A group of spring-born gilts (n = 30) were expected to reach puberty in autumn by the age of 7 months. Eighteen of these gilts were selected in pairs on the basis of matched days of birth. By the expected time, half of the animals showed oestrous symptoms (group CYCLING, n = 9) with the rest remaining silent (group SILENT, n = 9). Afterwards, all gilts were fitted with indwelling jugular catheters for frequent blood sampling. Blood samples were collected from all animals three times during the day followed by three times in the night at 2-h intervals for 48 h. The samples were analysed by a commercial radioimmunoassay (RIA). The results show a consistent 25-fold rise (on average) in night-time melatonin concentration in every animal sampled with group averages ranging from 0.28 +/- 0.04 to 0.37 +/- 0.06 pg/ml at day and from 10.20 +/- 2.16 to 10.67 +/- 0.05 pg/ml at night. Night-time group mean values between CYCLING and SILENT gilts did not differ significantly (10.26 +/- 0.67 and 10.38 +/- 0.94 for the CYCLING; 10.67 +/- 0.05 and 10.20 +/- 2.16 for the SILENT). When 10 pg/ml was used as a threshold value, six individuals did not reach it during the night (low responders). Two of these gilts were CYCLING and four were SILENT. In conclusion, the results presented imply no involvement of the level of night-time melatonin concentration in the seasonal delay of puberty in gilts. 相似文献
6.
An image-based stated choice approach was used to investigate the conditions determining why visitors to an urban forest in Vienna feel crowded or not. Respondents (N=213) evaluated several sets of images depicting trail use scenarios with different levels of social crowding conditions and several types of social interferences. Forest users were segmented into three groups based on their global evaluations of use levels during weekends and work days, resulting in a crowding-averse, a crowding-tolerant, and a crowding-indifferent segment. Crowding-averse respondents reacted much more negatively to scenarios with high-use levels, heterogeneous trail use conditions, and violations of personal minimum spatial requirements caused by the presence of others. This user group felt overcrowded because social conditions experienced in the area interfered with their main visiting goals, especially to walk with their dog unleashed and to recreate. By contrast, crowding-tolerant respondents disliked very low-use and high-use situations, and preferred a certain amount of social stimulation in the form of some encounters, and more heterogeneous trail use conditions. 相似文献
7.
ABSTRACT Trichoderma biocontrol isolates are most effective as highly concentrated inocula. Their antagonism to other fungi may be a result of pregermination respiration. In a nutrient-rich medium, almost all Trichoderma atroviride P1 (P1) conidia initiated germination processes and increased respiration, even in dense suspensions. When 1 x 10(7) P1 conidia/ml were coinoculated with 1 x 10(5) Botrytis cinerea conidia/ml, dissolved oxygen fell to <1% within 2 h and the pathogen failed to germinate. More dilute P1 suspensions consumed oxygen slowly enough to allow coinoculated B. cinerea to germinate. On nutrient-poor media, fewer P1 conidia initiated germination. Oxygen consumption by the inoculum and inhibition of B. cinerea were enhanced when P1 conidia were nutrient activated before inoculation. Pregermination respiration also affected competitive capacity of the antagonist on solid substrates, where respiratory CO(2) stimulated germination rate and initial colony growth. These parameters were directly correlated with inoculum concentration (R(2) >/= 0.97, P < 0.01). After initiating germination, Trichoderma conidia became more sensitive to desiccation and were killed by drying after only 2 h of incubation on a nutrient-rich substrate at 23 degrees C. These results indicate that nutrient-induced changes preceding germination in Trichoderma conidia can either enhance or decrease their biological control potential, depending on environmental conditions in the microhabitat. 相似文献
8.
Full pedigree quantitative trait locus analysis in commercial pigs using variance components 总被引:1,自引:0,他引:1
de Koning DJ Pong-Wong R Varona L Evans GJ Giuffra E Sanchez A Plastow G Noguera JL Andersson L Haley CS 《Journal of animal science》2003,81(9):2155-2163
In commercial livestock populations, QTL detection methods often use existing half-sib family structures and ignore additional relationships within and between families. We reanalyzed the data from a large QTL confirmation experiment with 10 pig lines and 10 chromosome regions using identity-by-descent (IBD) scores and variance component analyses. The IBD scores were obtained using a Monte Carlo Markov Chain method, as implemented in the LOKI software, and were used to model a putative QTL in a mixed animal model. The analyses revealed 61 QTL at a nominal 5% level (out of 650 tests). Twenty-seven QTL mapped to areas where QTL have been reported, and eight of these exceeded the threshold to claim confirmed linkage (P < 0.01). Forty-two of the putative QTL were detected previously using half-sib analyses, whereas 46 QTL previously identified by half-sib analyses could not be confirmed using the variance component approach. Some of the differences could be traced back to the underlying assumptions between the two methods. Using a deterministic approach to estimate IBD scores on a subset of the data gave very similar results to LOKI. We have demonstrated the feasibility of applying variance component QTL analysis to a large amount of data, equivalent to a genome scan. In many situations, the deterministic IBD approach offers a fast alternative to LOKI. 相似文献
9.
In this paper, the quantitative post-embryonic development of the Asian freshwater fish nematode Camallanus cotti Fujita, 1927, is described. Larval and adult morphometrics were obtained by following the parasite's life cycle experimentally using copepods Macrocyclops albidus (Jurine) as intermediate host and guppies Poecilia reticulata (Peters), southern platyfish Xiphophorus maculatus (Günther) and paradise fish Macropodus opercularis (L.) as definitive host. Additionally, adult worms were obtained from heavily infected paradise fish imported from Singapore. It is suggested that the gradual change in proportions of the worm's somatic body parts reflects the specific ecological role of each developmental stage. The free-living infective first-stage larva seems to be adapted for transmission, as indicated by its relatively long tail, designed to generate host-attracting movements, and its non-functional intestine. The second- and third-stage larvae from the copepod intermediate host seem mainly to invest in trophic functionality, i.e., the development of the buccal capsule and the oesophagus, which are crucial structures for the worm's successful establishment in the definitive fish host. Once in the fish intestine, the larvae enter a period of considerable growth. After the fourth (i.e., last) moult, a 72% increase in average female body length occurs. This is accompanied by doubling the average vulva-tail tip distance and the average tail length. The length of the female hind body expands in an accelerating allometric fashion, and seems to be closely linked to the posterior-wards expansion of the uterus. In the males however, growth seems to cease after the final moult. We conclude that female post-maturational body size, but especially the length of the hind body and the tail, are closely related to reproductive state, i.e., the developmental stage of the offspring in the uterus, and, probably, the worms' age. Any future taxonomical studies of camallanids in general, and C. cotti in particular, should thus be aware of the reproductive state of the females used. 相似文献
10.