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1.
Summary The competitive ability of inoculated and indigenous Rhizobium/Bradyrhizobium spp. to nodulate and fix N2 in grain legumes (Glycine max, Vigna unguiculata, Phaseolus vulgaris) and fodder legumes (Vicia sativa, Medicago sativa, and Trifolium subterraneum) was studied in pots with two local soils collected from two different fields on the basis of cropping history. The native population was estimated by a most-probable-number plant infectivity test in growth pouches and culture tubes. The indigenous rhizobial/bradyrhizobial population ranged from 3 to 2×104 and 0 to 4.4×103 cells g-1 in the two soils (the first with, the second without a history of legume cropping). Inoculated G. max, P. vulgaris, and T. subterraneum plants had significantly more nodules with a greater nodule mass than uninoculated plants, but N2 fixation was increased only in G. max and P. vulgaris. A significant response to inoculation was observed in the grain legume P. vulgaris in the soil not previously used to grow legumes, even in the presence of higher indigenous population (>103 cells g-1 soil of Rhizobium leguminosarum bv phaseoli). No difference in yield was observed with the fodder legumes in response to inoculation, even with the indigenous Rhizobium sp. as low as <14 cells g-1 soil and although the number and weight of nodules were significantly increased by the inoculation in T. subterraneum. Overall recovery of the inoculated strains was 38–100%, as determined by a fluorescent antibody technique. In general, the inoculation increased N2 fixation only in 3 out of 12 legume species-soil combinations in the presence of an indigenous population of rhizobial/bradyrhizobial strains. 相似文献
2.
Comparative analysis of organelle DNAs in acid citrus grown in Japan using PCR-RFLP method 总被引：1，自引：0，他引：1
Asad Asadi Abkenar Shiro Isshiki Ryoji Matsumoto Yosuke Tashiro 《Genetic Resources and Crop Evolution》2008,55(4):487-492
PCR-RFLP analyses of three regions for each of chloroplast DNA (cpDNA; rbcL-ORF106, trnD-trnT, trnH-trnK) and mitochondrial DNA (mtDNA; nad7/exon2-exon3, nad7/exon3-exon4, 18S-5S) were performed in 26 cultivars of acid citrus grown in Japan to identify polymorphisms and classify them. The polymorphisms
were compared with those of three true Citrus species, i.e., mandarin, pummelo and citron. Ichang papeda (C. ichangensis) was also included in this study to find its relationship with Yuzu. Inter-species cpDNA variation was recognized and the
acid citrus were divided into three groups, namely; I (‘Yuzukichi’ and ‘Kinkoyu’), II [sour oranges (‘Kaiseito’, ‘Daidai’
and ‘China daidai’), ‘Nansho daidai’, ‘Kiku daidai’, C. sudachi (‘Mushi yukaku’, ‘Yushi yukaku’ and ‘Yushi mukaku’), C. sphaerocarpa (‘Kabosu’ and ‘Aka kabosu’), C. kizu (‘Taninaka kizu’, ‘Kinosu’ and ‘Kizu’), ‘Zanbo’, ‘Mochiyu’, ‘Jabara’ and ‘Naoshichi’], and III [Yuzu (‘Tetraploid’, ‘Tochikei
yuzu’ and ‘Yamanekei yuzu’), ‘Matsuda sudachi’, ‘Zuishoyu’, ‘Hanayu’ and ‘Yuko’]. CpDNA restriction patterns of the three
true Citrus species differed from each other as well as from those of ichang papeda. CpDNA restriction patterns of group I of the acid
citrus were identical to those of mandarins. Group II showed the same as pummelos. CpDNA restriction patterns of group III
were differed from those of the three true Citrus species in the three regions. This group was differed from ichang papeda after digestion of trnH-trnK PCR products with TaqI, HinfI and AluI, while they showed identical restriction patterns in two regions, rbcL-ORF106 and trnD-trnT. Citrons and ichang papeda were placed in groups IV and V, respectively. Based on mtDNA restriction patterns, the acid citrus
were divided into three groups; i, ii and iii. In groups i and ii accessions of groups I and II of cpDNA were placed with
mandarins and pummelos, respectively. In group iii accessions of group III of cpDNA were placed with ichang papeda. Citrons
were placed in a distinct group, iv. 相似文献
3.
Md. Masud Karim Nazmoon Naher Tonu Mohammad Shakhawat Hossain Taketo Funaki M. Bahadur Meah Delwar M. Hossain M. Asad ud-doullah Eigo Fukai Keiichi Okazaki 《Euphytica》2016,208(3):535-544
Low erucic acid (LEA) rapeseed, which has accumulated mutant fatty acid elongase genes at the BnFAE1.1 and BnFAE1.2 loci of the A- and C-genome, respectively, is an important oilseed crop. Short growing turnip rape (B. rapa) is also important as a catch crop in the continuous cropping of rice in Asia but there is no LEA B. rapa cultivar for cultivation in South Asia. In order to develop LEA turnip rape cultivars, high erucic acid turnip rape cultivars were interspecifically crossed as recurrent parents to a canola quality rapeseed. In the meantime, we monitored incorporation of the mutant bnfae1.1 (e1) gene into A-genome of turnip rape, using a dCAPS primer pair, which can amplify PCR fragment only for the mutant e1 gene from A-genome. The early backcross progenies showed poor seed set, but which was improved in advanced progenies. Finally, homozygous e1e1 genotypes were established in the selfed progenies of BC2–BC3, and their LEA content was confirmed by gas-chromatography analysis. Our results and promising lines will contribute to LEA-trait selection in turnip rape and rapeseed breeding. 相似文献
4.
Parthenium hysterophorus in Nepal: a review of its weed status and possibilities for management 
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下载免费PDF全文 The rapid expansion of the alien invasive Parthenium hysterophorus is a new agricultural and environmental problem for Nepal. Although the weed was first recorded in 1967, the most significant expansion has occurred in the last 20 years. The weed is thought to have entered Nepal from India and currently is found in the Tarai, Siwalik and hill regions of Nepal. A CLIMEX modelling projection has shown these regions to be climatically suitable for the growth of P. hysterophorus, both under the present and a future projected increased temperature. From the initially invaded roadside vegetation, the weed has now spread into cropping land and forests. Vehicle movement and transportation of agriculture products are the main means by which its seed is spreading. The weed has reduced plant species richness and changed species composition in grasslands, while fodder collectors have developed contact dermatitis from the plant. To date, no sustainable management strategy has been developed for this weed. However, the fortuitous arrival of the biological control agents Zygogramma bicolorata and Puccinia abrupta var. partheniicola has had some impact upon the weed's growth and abundance. A CLIMEX modelling projection identifies many suitable locations for Z. bicolorata outside of its present range and within areas where the weed is likely to spread, both under the present and under a future projected climate of increased temperature. Mapping of the currently invaded areas, management through community involvement and further releases of new and redistribution of current biological control agents are required to manage P. hysterophorus in Nepal. 相似文献
5.
Somayeh Pouralimotlagh Asad Mohammadi Zarejabad Ehsan Ahmadifar 《Journal Of Applied Aquaculture》2013,25(1):74-85
This study investigates the effects of replacing fish oil with two vegetable oils (Sunflower oil and Soybean oil) on growth performance, salinity tolerance, hematological and serum biochemical profile of Rutilus rutilus over 56 days. Five diets were supplemented with 8% of either fish oil (FO) (control), sunflower oil (SFO), and soybean oil (SO) or a combination of 4% FO with 4% SFO and 4% FO with 4% SO, respectively. Triplicate groups of fish (24 fish per tank with an average body weight, of 5.52 g) were assigned to each diet. Blood samples were taken from the caudal vein of 75 apparently healthy fish at the end of trial. Hematological values of the blood samples were determined using standard techniques. At the end of the experiment fish were exposed to salinity stress. The results showed that the best growth performance was in fish fed the control diet, but the differences were minor among treatments containing vegetable oils (P > 0.05). Serum cholesterol and glucose concentrations were significantly higher in fish fed dieta containing vegetable oils (P < 0.05). Red blood cell count (RBC), hematocrit (Ht) and hemoglobin (Hb) in groups fed diets containing SFO and SO were higher than other groups (P < 0.05). There was no significant difference in survival after 48 h salinity stress (P > 0.05). These results indicate that in cases where fish oil is scarce or expensive, vegetable lipids can be used as replacements without any serious negative growth performance and health impacts. 相似文献
6.
Shahid Iqbal Awan Muhammad Shahzad Ahmed Jehanzeb Farooq Syed Dilnawaz Ahmad Muhammad Ilyas Asad Hussain Shah Muhammad Fareed Khan Sardar Ali Lutful Hasan 《Frontiers of Agriculture in China》2011,5(4):486-496
An experiment was conducted to access the genetic variability among early vigour and quantitative traits under limited moisture
in F2 generation of a 5 × 5 diallel cross of bread wheat. The results indicated that there was significant genotypic variation
among the genotypes. Additive dominance model revealed full fitness of the data for RL, DSW, FRW, DRW, spike length, and 1000-grain
weight but was partially fit for SL, FSW, R/S, tillers per seedling, plant height, tillers per plant, spikelets per spike,
and grain yield per plant. The partially adequate models for these plant characters might be due to the presence of non-allelic
interaction, linkage, and non-independent distribution of the genes in the parents. Additive genes coupled with moderate to
high narrow sense heritability were involved in the heritage of all the traits, which indicated a higher scope of selection
in early generations. 相似文献
7.
Fahimeh Hajipour Sedigheh Asad Mohammad Ali Amoozegar Ali Asghar Javidparvar Jialun Tang Haizheng Zhong Khosro Khajeh 《Iranian Biomedical Journal》2021,25(1):8
Background:Azo dyes are the most widely used synthetic colorants in the textile, food, pharmaceutical, cosmetic, and other industries, accounting for nearly 70% of all dyestuffs consumed. Recently, much research attention has been paid to efficient monitoring of these hazardous chemicals and their related metabolites because of their potentially harmful effect on environmental issues. In contrast to the complex and expensive instrumental procedures, the detection system based on the QDs with the superior optochemical properties provides a new era in the pollution sensing and prevention. Methods:We have developed a QD-enzyme hybrid system to probe MR in aqueous solutions using a fluorescence quenching procedure. Results:The azoreductase enzyme catalyzed the reduction of azo group in MR, which can efficiently decrease the FRET between the QDs and MR molecules. The correlation between the QDs photoluminescence recovery and MR enzymatic decolorization at the neutral phosphate buffer permitted the creation of a fluorescence quenching-based sensor. The synthesized biosensor can be used for the accurate detection of MR in a linear calibration over MR concentrations of 5-84 μM, with the LOD of 0.5 μM in response time of three minutes. Conclusion:Our findings revealed that this fluorometric sensor has the potential to be successfully applied for monitoring a wide linear range of MR concentration with the relative standard deviation of 4% rather than the other method. Key Words: Azoreductase, Methyl red, Quantum dots 相似文献
8.
AbstractSoil organic carbon (SOC) sequestration is one of the major agronomic measures to mitigate green house gas emission, enhance food security, and improve agriculture sustainability. The study, therefore, aimed to evaluate crop growth (CG) and radiation use efficiency in spring wheat (Triticum aestivum L.) treated soil with residue type (RT), that is, cowpea (Vigna unguiculata) as legume (LR), maize (Zea mays L.) as cereal (CR) and no residue (NR) treatment applied (5 t ha?1) on dry matter basis. The CR was subsequently incorporated with tillage depths (TD), that is, deep (DT?=?35?cm) and shallow (ST?=?15?cm) as main plot treatments. The N was applied in two splits starting from 0 to 160?kg ha?1 as sub plot treatments. Experiment was conducted in two CG seasons 2009–11 at Agronomy Research Farm, the University of Agriculture Peshawar, Pakistan. Results showed the highest CG and RUE with LR incorporated than CR and/or NR with DT. Increasing N-rate resulted an increase in CG, RUE and biomass of wheat. Residue of LR or CR deeply incorporate into the soil has resulted healthy traits (i.e., tillers- and spikes number), which resulted higher biomass. Nitrogen applied 120?kg ha?1 resulted in higher CG, RUE and grain yield for treatment LR, followed by CR and the lowest for the NR. Crop of second year showed higher grain yield, which was due to healthy traits including better CG and RUE. The study suggests that CR of LR or CR nature incorporated deep into the soil can optimize crop N-fertilizer demand for optimum production, which protects environment from the excessive use of N application. 相似文献
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Fahim Nawaz R. Ahmad E. A. Waraich M. S. Naeem R. N. Shabbir 《Journal of plant nutrition》2013,36(6):961-974
The response of different wheat cultivars to drought imposed after three and six weeks of seedling emergence was evaluated in the wire house. The seeds of recommended local wheat cultivars were sown in plastic pots. The drought stress decreased the water relation, nutrient uptake and grain yield of all the wheat cultivars. The early drought stress significantly reduced the nitrogen (N) uptake by 38% while late drought stress decreased nitrogen uptake by 46%. The phosphorus (P) and potassium (K) uptake were decreased by 49% and 37% under early drought stress, respectively while their uptake was decreased by 51% each under late drought stress. Grain yield was reduced by 24% under early drought stress while it was reduced by 60% under late drought stress. Water deficit at early growth stages reduced grain weight by 10% while it was reduced by 35% under water deficit at later stages of growth. 相似文献