Correlation between surface free energy and surface constitution     

Chaudhury MK  Whitesides GM 《Science (New York, N.Y.)》1992,255(5049):1230-1232

Self-assembled monolayers (SAMs) of alkylsiloxanes on elastomeric PDMS (polydimethylsiloxane) were used as model systems to study interactions between surfaces. Surface free energies (gammasv) of these chemically modified surfaces were estimated by measuring the deformations that resulted from the contact between small semispherical lenses and flat sheets of the elastomer under controlled loads. The measured surface free energies correlated with the surface chemical compositions of the SAMs and were commensurate with the values estimated from the measurements of contact angles. This study provides direct experimental evidence for the validity of estimates of the surface free energies of low-energy solids obtained from contact angles.  相似文献   

Identification of internal control genes in milk‐derived mammary epithelial cells during lactation cycle of Indian zebu cow          下载免费PDF全文

Pradeep Jatav  Monika Sodhi  Ankita Sharma  Sandeep Mann  Amit Kishore  Umesh K Shandilya  Ashok K Mohanty  Ranjit S Kataria  Poonam Yadav  Preeti Verma  Surinder Kumar  Dhruba Malakar  Manishi Mukesh 《Animal Science Journal》2016,87(3):344-353

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Interactive effects of Cd and PAHs on contaminants removal from co-contaminated soil planted with hyperaccumulator plant <Emphasis Type="Italic">Sedum alfredii</Emphasis>     

Kai Wang  Zhiqiang Zhu  Huagang Huang  Tingqiang Li  Zhenli He  Xiaoe Yang  Ashok Alva 《Journal of Soils and Sediments》2012,12(4):556-564

Purpose  

Soil contamination by multiple organic and inorganic contaminants is common but its remediation by hyperaccumulator plants is rarely reported. The growth of a cadmium (Cd) hyperaccumulator Sedum alfredii and removal of contaminants from Cd and polycyclic aromatic hydrocarbons (PAHs) co-contaminated soil were reported in this study.  相似文献   

Direct determination of p-nitrophenyl substituent organophosphorus nerve agents using a recombinant Pseudomonas putida JS444-modified Clark oxygen electrode     

Lei Y  Mulchandani P  Chen W  Mulchandani A 《Journal of agricultural and food chemistry》2005,53(3):524-527

A microbial biosensor for rapid, sensitive, selective, and cost-effective determination of the total content of organophosphorus nerve agents with p-nitrophenyl substituent is reported. The biosensor consisted of genetically engineered PNP-degrader Pseudomonas putida JS444 expressing organophosphorus hydrolase (OPH) on its cell surface immobilized on a dissolved oxygen electrode. Surface-expressed OPH catalyzed the hydrolysis of organophosphorus pesticides with p-nitrophenyl substituent such as paraoxon, methyl parathion, and parathion to release p-nitrophenol that was oxidized by the enzymatic machinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen. The oxygen consumption was measured and correlated to the concentration of organophosphates. The sensor signal and response time were optimized with 0.086 mg dry weight of cell and operating in 50 mM pH 7.5 citrate-phosphate buffer with 50 microM CoCl(2) at room temperature. When operated at optimized conditions, the biosensor measured as low as 55 ppb of paraoxon, 53 ppb of methyl parathion, and 58 ppb of parathion without interference from most phenolic compounds and other commonly used pesticides, such as atrazine, coumaphos, sutan, sevin, and diazinon. The operational life of the microbial biosensor was approximately 5 days when stored in the operating buffer at 4 degrees C.  相似文献   

Comparative sensitivity of barley (Hordeum vulgare L.) to insecticide and fungicide on different stages of cell cycle     

Parul Singh  Anjil Kumar Srivastava  Ashok Kumar Singh 《Pesticide biochemistry and physiology》2007,89(3):216-219

In this study, the comparison of cytogenetic effects of insecticide and fungicide in different phases of cell cycle was investigated in the root tip cells of barley (Hordeum vulgare L.). The seeds of H. vulgare L. Var. Karan 16 were treated with different concentrations (0.05%, 0.1% and 0.5%) of insecticide Profenophos (PF) and fungicide Mancozeb (MZ) for 6 h after presoaking durations of 7, 17 and 27 h.The different presoaking durations were used to bring the cells in various phases of cell cycle. Negative control was run parallel in distilled water. Cytogenetic examinations of root meristems exposed to the PF and MZ showed significant inhibition of mitotic index (MI) as well as significant increase in chromosomal aberrations (CAs). These parameters were dependent on the concentrations of insecticide and fungicide. The present study shows that PF and MZ both caused more damage in S phase of cell cycle which indicates that S phase is more sensitive in comparison to other phases.  相似文献   

Alterations in oxidant/antioxidant balance,high-mobility group box 1 protein and acute phase response in cross-bred suckling piglets suffering from rotaviral enteritis     

Ujjwal Kumar De  Reena Mukherjee  Sukdeb Nandi  Bhimnere Hanumatnagouda Manjunatha Patel  Umesh Dimri  Chintu Ravishankar  Ashok Kumar Verma 《Tropical animal health and production》2014,46(7):1127-1133

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Understanding genetic diversity in blackgram [Vigna mungo (L.) Hepper] collections of Indian National Genebank     

Gayacharan  Tripathi  Kuldeep  Aski  Muraleedhar S.  Singh  Neeta  Kumar  Ashok  Lal  Hanuman 《Genetic Resources and Crop Evolution》2022,69(3):1229-1245

Genetic Resources and Crop Evolution - Blackgram [Vigna mungo (L.) Hepper] is a protein rich pulse crop of Indian origin. The crop is cultivated in diverse range of agro-ecological regions,...  相似文献   

Optimization of Factors Affecting <Emphasis Type="Italic">In vitro</Emphasis> Establishment, <Emphasis Type="Italic">Ex vitro</Emphasis> Rooting and Hardening for Commercial Scale Multiplication of Silk Banana (<Emphasis Type="Italic">Musa</Emphasis> AAB)     

A. A.?WamanEmail author  Pooja?Bohra  B. N.?Sathyanarayana  K.?Umesha  G. K.?Mukunda  T. H.?Ashok  Balakrishna?Gowda 《Erwerbs-Obstbau》2015,57(3):153-164

Factors concerning aseptic culture establishment and hardening were studied in detail in choicest Silk Banana. Effect of size of sucker (small, medium and large), carbon concentration (10, 20 and 30 g/l), season of initiation (wet and dry) and pre-treatments such as segmentation and incision to the explants were studied during initiation. Further, hardening related factors such as substrates used for primary and secondary hardening, nursery nutrition (source and frequency of application) and pre-treatments for ex vitro rooting were also studied. Results revealed that small suckers were most suited for initiations with the least contamination, maximum establishment and higher percentage of greening. Lower concentration of sucrose, though delayed greening, resulted in 100?% establishment of explants. Initiations performed during the drier period were completely free from the fungal contamination and showed less bacterial contamination than those performed during the rainy season. Segmentation of explant into four parts during first subculture supported maximum shoot proliferation by overcoming apical dominance. Coir pith was observed to be the most congenial substrate during primary hardening, whereas coir pith alone or sand: red earth: coir pith (1:1:1) supported superior performance of plantlets during secondary hardening. Single application of mono ammonium phosphate improved growth of plantlets during secondary hardening. Pre-treatment with mono ammonium phosphate gave the best response in terms of rooting and hardening of un-rooted micro-shoots. Thus, the discussed methodology could help the industries to take up commercial scale propagation of Silk Banana.  相似文献   

Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers          下载免费PDF全文

Kapil K. Tiwari  Anshuman Singh  Sasmita Pattnaik  Maninder Sandhu  Sukhdeep Kaur  Sourabh Jain  Sushma Tiwari  Shweta Mehrotra  Mahender Anumalla  Rashmita Samal  Jyoti Bhardwaj  Neha Dubey  Vikrant Sahu  Gayle A. Kharshing  Patu K. Zeliang  Kadiri Sreenivasan  Pankaj Kumar  Swarup K. Parida  Sevanthi V. A. Mithra  Vandana Rai  Wricha Tyagi  Pawan K. Agrawal  Atmakuri R. Rao  Arunava Pattanayak  Girish Chandel  Ashok K. Singh  Ishwari S. Bisht  Kangila V. Bhat  Gundimeda J. N. Rao  Jitendra P. Khurana  Nagendra K. Singh  Trilochan Mohapatra 《Plant Breeding》2015,134(2):164-171

Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining.  相似文献   

Collection and Characterization of Citrus indica Tanaka and C. macroptera Montr.: Wild Endangered Species of Northeastern India     

S. K. Malik  R. Chaudhury  O. P. Dhariwal  Rajwant K. Kalia 《Genetic Resources and Crop Evolution》2006,53(7):1485-1493

Citrus indica and C. macroptera are the wild endangered species of Citrus occurring in northeastern India. Surveys were undertaken in this region for ascertaining distribution, studying variability and for collection of germplasm of these two species. C. indica, an endemic species of this region, was collected from the Citrus Gene Sanctuary located in buffer zone of Nokrek Biosphere Reserve in the Garo hills of Meghalaya. In addition, a putative natural hybrid of C. indica and C. limon was collected for the first time from the south Garo hills. C. macroptera had much wider distribution and was collected from Mizoram and Meghalaya states. In Jantia hills of Meghalaya, natural populations of this species are in a highly threatened state. The two species were unevenly distributed all over the explored territory. Morphological characterization of leaves, fruits and seeds indicated the presence of sizable variability within collected accessions of these two Citrus species. Indigenous technical knowledge gathered on the use and socio-economic importance indicated commercial potential for these two species in northeastern India. However, lack of cultivation of these species and clearing of forest cover at an alarming rate has led to an urgent need to adopt complementary conservation strategies to safeguard these species and to ensure their availability for future utilization. A major emphasis on developing methods for their propagation, multiplication and regeneration in in situ and ex situ conditions is required.  相似文献