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1.
Aleksa Obradovic Athanassios Mavridis Klaus Rudolph Jaap D. Janse Momcilo Arsenijevic Jeffrey B. Jones Gerald V. Minsavage Jaw-Fen Wang 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(3):285-292
During the last two decades bacterial strains associated with necrotic leaf spots of pepper and tomato fruit spots were collected in Serbia. Twenty-eight strains isolated from pepper and six from tomato were characterized. A study of their physiological and pathological characteristics, and fatty acid composition analysis revealed that all of the strains belong to Xanthomonas campestris pv. vesicatoria. Being non-amylolytic and non-pectolytic, pathogenic on pepper but not on tomato, containing lower amounts of fatty acid 15 : 0 ante–iso, the pepper strains were designated as members of the A group of X. campestris pv. vesicatoria. However, the tomato strains hydrolyzed starch and pectate, caused compatible reactions on tomato but not on pepper, had higher percent of 15 : 0 ante–iso fatty acid, and were classified into B phenotypic group and identified as X. vesicatoria. PCR primers were developed which amplified conserved DNA regions related to the hrp genes of different strains of X. campestris pv. vesicatoria associated with pepper and tomato. Restriction analysis of the PCR product resulted in different patterns and enabled grouping of the strains into four groups. When xanthomonads isolated from pepper and tomato in Serbia were analyzed, they clustered into two groups corresponding to the grouping based on their physiological and pathological characteristics. According to the reaction of pepper and tomato differential varieties, the strains from pepper belong to races P7 and P8 and tomato strains belong to the race T2. All strains were sensitive to copper and streptomycin. Advantages and disadvantages of various bacterial spot management practices are discussed. 相似文献
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Kaldrymidou E Papaioannou N Poutahidis T Karayannopoulou M Gruys E Toliou T Tsangaris T 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2000,47(8):457-462
A case of a canine large cell type T-cell lymphoma, with features of high-grade malignancy is described. The tumour was found confined in the nasal cavity and the paranasal sinuses of a crossbred German Shepherd dog. Histological examination revealed the features of a highly malignant large cell lymphoma. Ultrastructurally, the lymphoid tumour cells bore cytoplasmic protrusions that interdigitated tightly. From a panel of tumour markers used, the neoplastic cells were stained only for vimentin. Immunophenotyping of the tumour cells by means of CD3, CD79, kappa-light chains and lambda-light chains detection was undertaken. The tumour stained only for CD3 and was classified as T-cell lymphoma. 相似文献
4.
Stergios Gakis Konstantinos Mantzanas Dimitrios Alifragis Vasilios P. Papanastasis Athanasios Papaioannou Dimosthenis Seilopoulos Panagiotis Platis 《Agroforestry Systems》2004,60(2):149-157
The interaction between understorey plants and trees in a young silvopastoral system was studied in a sub-humid Mediterranean
environment with cold winters in northern Greece. The experimental design was a split-split plot with three replications and
included: three understorey treatments (grass, legume and control), two tree species (Acer pseudoplatanus and Pinus sylvestris) and two spacings (2.5×2.5 m and 3.5×3.5 m). The competition between herbaceous plants and trees resulted in significant
differentiation in tree growth while their spacing did not produce any significant differences. The influence of trees on
herbage yield either by plant species or spacing was not significant. During the early stages of establishment, a significant
positive correlation was observed between sycamore growth and soil moisture, leaf weight, leaf area and leaf number as well
as nitrogen and potassium concentration in leaves. Six years after planting the height increase of the sycamore trees was
largest in the control treatment (415%), lowest in the grass treatment (134%) and intermediate (192%) in the legume treatment.
Much higher was the increase obtained for the diameter, 161%, 207% and 536% respectively for the grass, legume and control
treatments. The Scots pine trees grew faster than sycamore over the course of the experiment. Height increased by 397%, 351%
and 400% and diameter by 518%, 443% and 683% respectively for the grass, legume and control treatments.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
5.
Depletion of CD4+ T cells in major histocompatibility complex class II-deficient mice 总被引：32，自引：0，他引：32
M J Grusby R S Johnson V E Papaioannou L H Glimcher 《Science (New York, N.Y.)》1991,253(5026):1417-1420
The maturation of T cells in the thymus is dependent on the expression of major histocompatibility complex (MHC) molecules. By disruption of the MHC class II Ab beta gene in embryonic stem cells, mice were generated that lack cell surface expression of class II molecules. These MHC class II-deficient mice were depleted of mature CD4+ T cells and were deficient in cell-mediated immune responses. These results provide genetic evidence that class II molecules are required for the maturation and function of mature CD4+ T cells. 相似文献
6.
Tsakos P Siarkou V Guscetti F Chowdhury H Papaioannou N Vretou E Papadopoulos O 《Veterinary microbiology》2001,82(3):285-291
Two groups of pregnant ewes were experimentally infected oronasally in midpregnancy. A faecal and an abortion-source isolate of Chlamydophila abortus were used. They were derived from a healthy ewe from a flock with no history of abortion, and from an aborted foetus in a farm with enzootic abortion. As assessed by modified Ziehl-Neelsen (MZN) staining, egg culture, antigen ELISA, the Clearview test and immunohistochemistry, inoculation resulted in placental and/or foetal infection in all ewes. Histopathology revealed placentitis in two and four ewes inoculated with the enteric or abortion-source isolate, respectively, in addition, these samples were immunohistochemically positive for chlamydial antigen. All six ewes infected with the enteric isolate and five of seven ewes infected with the abortion-source isolate showed evidence for a serological response by an indirect ELISA or CFT. Neither chlamydiae nor lesions were detected in the placentae and lambs of the uninfected control ewes, which remained seronegative. Our results suggest that enteric C. abortus can be associated with placental and foetal lesions in sheep. 相似文献
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K. V. Papageorgiou M. Chiotelli E. Panteris N. Papaioannou H. Nauwynck S. K. Kritas 《Anatomia, histologia, embryologia》2017,46(1):58-64
Myelination, the ensheathing of neuronal axons by myelin, is important for the proper function of both central and peripheral nervous systems. Various studies have investigated the quantitative parameters of myelination in certain species. Pigs are among the species of which their use as laboratory animals in neuroscience research increased the past few decades. However, there is limited data regarding the myelination process in the pig. Moreover, the maxillary nerve is crucial for Pseudorabies Virus (PrV) neuropathogenesis. In this context, a quantitative analysis of various myelination parameters of the maxillary nerve was performed, during the first 5 weeks of porcine post‐natal development, the time period, which exhibits the highest interest for PrV neuropathogenesis. The evaluation was conducted in four groups of uninfected pigs, at the time of birth (group 0w), at the age of 1 week (group 1w), 3 weeks (group 3w) and 5 weeks (group 5w), using toluidine blue staining, immunofluorescence and electron microscopy. Axon and fibre diameter, perimeter and surface, myelin sheath thickness and g‐ratio were measured on histological sections transverse to the longitudinal axis of the maxillary nerve. The thickness of myelin sheath was 0.76 μm for group 0w, 0.94 μm for group 1w, 0.98 μm for group 3w and 1.03 μm for group 5w. The g‐ratio was 0.529, 0.540, 0.542 and 0.531 for the respective animal groups. The results of this study contribute to the understanding of the myelination process in the pig will be used for the study of PrV effects on the myelination development of newborn piglets' maxillary nerve and may shed new light to their vulnerability to the virus. 相似文献
8.
Stavros Sakellariou Stergios Tampekis Fani Samara Athanassios Sfougaris Olga Christopoulou 《林业研究》2017,28(6):1107-1117
Forest ecosystems are our priceless natural resource and are a key component of the global carbon budget. Forest fires can be a hazard to the viability and sustainable management of forests with consequences for natural and cultural environments, economies, and the life quality of local and regional populations. Thus, the selection of strategies to manage forest fires, while considering both functional and economic efficiency, is of primary importance. The use of decision support systems (DSSs) by managers of forest fires has rapidly increased. This has strengthened capacity to prevent and suppress forest fires while protecting human lives and property. DSSs are a tool that can benefit incident management and decision making and policy, especially for emergencies such as natural disasters. In this study we reviewed state-of-the-art DSSs that use: database management systems and mathematical/economic algorithms for spatial optimization of firefighting forces; forest fire simulators and satellite technology for immediate detection and prediction of evolution of forest fires; GIS platforms that incorporate several tools to manipulate, process and analyze geographic data and develop strategic and operational plans. 相似文献
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Sobhi M. Girgis Frederic P. Bem Chrysostomos I. Dovas Athanassios Sclavounos Apostolos D. Avgelis Mina Tsagris Nikos Katis Panayota E. Kyriakopoulou 《European journal of plant pathology / European Foundation for Plant Pathology》2009,125(2):203-211
Unusual symptoms were observed on ‘Baresana’ x ‘Baresana’ Vitis vinifera hybrid vines in the Grapevine Variety Collection of the Grapevine Institute, Athens. The affected vines showed sharp angular
mosaic on leaves, along the veins and in vein angles, malformations, abortive flowers or very few berries with smaller, wrinkled
and non-germinating seeds, as well as gradual decline, severe stunting and death of the vine. Serological tests on diseased
vines for the presence of 13 known grapevine viruses gave negative results. An infectious agent was transmitted mechanically
to several herbaceous indicator plants. Koch’s Postulates were fulfilled, and the agent, proven to be a virus, was named Grapevine angular mosaic virus (GAMV). Serological tests have been developed for the virus. The most conserved polymerase region showed significant similarity
of GAMV with members of subgroup 1 of the Ilarvirus genus; however ML phylogenetic analysis could not support its clustering within this subgroup. GAMV differs serologically
and in particle morphology from Grapevine line pattern virus (GLPV) a putative member of the Ilarvirus genus that infects grapevine. It is proposed that GAMV is a novel member of the Ilarvirus genus. 相似文献
10.
KK Adamama-Moraitou D Pardali NN Prassinos LG Papazoglou D Makris KI Gourgoulianis N Papaioannou TS Rallis 《Australian veterinary journal》2010,88(9):351-356
Objectives To investigate whether there are any changes in the tidal breathing flow volume loop (TBFVL) in calm, non-dyspnoeic dogs with intratracheal masses. Methods We compared 4 dogs with intratracheal masses (group 1) with 10 healthy dogs (group 2). Routine clinical and laboratory examinations of the dogs were unremarkable, except for episodic upper respiratory obstructive signs in the dogs in group 1. Lateral radiography of the neck and thorax showed that group 1 dogs had masses that appeared to protrude into the tracheal lumen. Tracheoscopy and surgery or necropsy was performed to confirm the presence of the mass. Arterial blood gas and TBFVL analysis was carried out in all dogs to assess respiratory status. Results The shape of the TBFVL for dogs in group 1 was narrower and ovoid compared with that for the group 2 dogs. Tidal volume and expiratory and inspiratory times were significantly reduced, whereas the respiratory rate was increased for dogs in group 1 compared with dogs in group 2. Arterial blood gas analysis was unremarkable for all dogs. Conclusions TBFVL is a non-invasive technique that is easy to perform and well tolerated by dogs. In the absence of abnormalities detected by routine diagnostic evaluations and arterial blood gas analysis in dogs with intratracheal masses, the TBFVL contributes to the definition of the physiologic status of the airways at the time of testing, and results suggests that these dogs breathe quite normally when they are calm and non-dyspnoeic. 相似文献