Genetic analysis of selected almond genotypes and cultivars grown in Turkey using peroxidase-gene-based markers     

Hasan Pinar  Mustafa Unlu  Sezai Ercisli  Aydin Uzun  Mustafa Bircan 《林业研究》2016,27(4):747-754

Peroxidase plays important roles in many stress-related interactions and catalyzes important reactions in various physiological processes. Since peroxidase played critical roles in the evolution of almond(Prunus dulcis Miller(D.A Webb) syn P. amygdalus Batsch), peroxidase-gene-based analyses may increase the understanding of evolution of this species. Peroxidase gene polymorphism(POGP) markers were used to determine genetic diversity and relationships among 69 Turkish genotypes/cultivars and 27 foreign almond cultivars by using unweighted pair group method with arithmetic mean(UPGMA) analysis. This study is the first evaluation of the use of POGP markers for diversity analysis in almond.Totally, 83 fragments were obtained from eight peroxidase primer pairs, and polymorphism was identified as 94 %.Similarity level among the genotypes ranged between 0.63 and 0.93, and all materials were distinguished. In general,Turkish and foreign genotypes were mixed in clusters since they share a common genetic background and gene migration among the sites. Clusters were not based on geographic regions except for some minor groupings. This study indicated that peroxidase gene markers can be reliably used to determine genetic relationships in almonds.  相似文献   

The effect of various nitrogen fertilizers on zinc (zn) availability in non calcic brown forest soils     

Aydin Adiloglu  Sevinç Adiloglu  Esin Gonulsuz  Nureddin Oner 《Archives of Agronomy and Soil Science》2013,59(6):601-606

In this article the effect of different nitrogen fertilizer application on the availability of zinc (Zn) content in non calcic brown forest soil was investigated. For this purpose increasing rates of NH₄Cl, (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 fertilizers were applied for having different physical and chemical properties in two soil samples. An incubation experiment was carried out over 4 months. The available Zn content of the soils was determined each month. According to the results the highest available Zn content of the soils was obtained from NH₄Cl applied to the soils followed by (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 application to soil. At the end of the experiment the average available Zn content of non calcic brown forest soils for NH₄Cl, (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 fertilizers are 0.93, 0.91, 0.88 and 0.77 mg kg^?1, respectively.  相似文献   

Effect of Water Stress at Various Growth Stages on Some Quality Characteristics of Winter Wheat   总被引：8，自引：0，他引：8  

A. Ozturk  F. Aydin 《Journal of Agronomy and Crop Science》2004,190(2):93-99

A field experiment was carried out in Erzurum (Turkey) on winter wheat to analyse the effect of water stress at different growth stages – fully irrigated (FI), rainfed (R), early water stress (EWS), late water stress (LWS) and continuous water stress (CWS), on some quality characteristics – in the 1995–96 and 1996–97 cropping seasons. Water stress had a substantial effect on most of the quality characteristics. As averages of cropping seasons, CWS, EWS, R and LWS treatments decreased grain yields by 65.5, 40.6, 30.5 and 24.0 %, respectively, compared with the FI treatment. CWS increased grain protein content by 18.1 %, sedimentation volume by 16.5 %, wet gluten content by 21.9 % and decreased 1000‐kernel weight by 7.5 g compared with FI treatment. LWS caused an increase of 8.3 % in grain protein content, 8.7 % in sedimentation volume, 10.8 % in wet gluten content and a reduction of 3.8 g in 1000‐kernel weight compared with FI. EWS and R increased sedimentation volume and wet gluten content, but decreased 1000‐kernel weight compared with FI. The effect of LWS on grain quality was more significant than that of EWS. The results suggest that soil moisture conditions increase grain yield and kernel weight of winter wheat but decrease its quality.  相似文献   

Determination of suitable chemical extraction methods for the available iron content of brown forest soils in Turkey     

Aydin Adiloglu 《Eurasian Soil Science》2006,39(9):961-967

The aim of this research was to determine the available iron (Fe) content of brown forest soils of Edirne Province and the most suitable chemical extraction method. Eight chemical extraction methods (the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 MTEA, 0.05 M HCl + 0.012 M H2SO4, 1 M NH4OAc (pH: 4.8), 0.01 M EDTA + 1 M NH4OAc, 1 M MgCl2, 0.01 M EDTA + 1 M (NH4)2CO3, 0.005 M DTPA + 1 M NH4HCO3, and 0.001 M EDDHA methods) and six biological indices (the dry matter yield, Fe concentration, Fe uptake, relative dry matter yield, relative Fe concentration, and relative Fe uptake) were compared. The biological indices were determined with barley (Hordeum vulgare L.) grown under greenhouse conditions. At the end of the experiment, the highest correlation coefficients (r) were determined to be between the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA method and the biological indices and between the 0.005 M DTPA + 1 M NH4HCO3 method and the biological indices. The corresponding correlation coefficients (r) for the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA method and the six biological indices were 0.621**, 0.823**, 0.810** 0.433**, 0.558**, and 0.640**, respectively. For the 0.005 M DTPA + 1 M NH4HCO3 method, these coefficients were equal to 0.618**, 0.520**, 0.679**, 0.521**, 0.492**, and 0.641**, respectively (** indicate the validity of the relationships at p < 0.01) These extraction methods, out of all the methods tested, were suggested for the determination of the available Fe content of the brown forest soils. Published in Russian in Pochvovedenie, 2006, No. 9, pp. 1068–1074. The text was submitted by the author in English.  相似文献   

Genotypic variation in phosphorus efficiency between wheat cultivars grown under greenhouse and field conditions     

Aydin GUNES  Ali INAL  Mehmet ALPASLAN  Ismail CAKMAK 《Soil Science and Plant Nutrition》2006,52(4):470-478

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Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey     

Turan N  Yildirim F  Altan E  Sennazli G  Gurel A  Diallo I  Yilmaz H 《Research in veterinary science》2012,93(3):1504-1507

The aim of the present study was to investigate abortion storms that occurred in the Marmara region of Turkey in 2008-2009 using a real-time PCR. Two aborted foetuses were necropsied and histo-pathological findings reported herein. Ten lungs, 3 brains and one nasal swab from 10 aborted foetuses, 6 nasal swabs and 3 vaginal swabs from aborting mares were included in this study. EHV-1 was isolated from the lung, liver and brain of 1 aborted foetus. EHV-1 DNA was detected in the lungs, livers and spleens of 2 necropsied foetuses and in 3 lungs from 10 foetuses submitted for diagnosis. A brain from one of the aborted foetuses was also positive for EHV-1 DNA. EHV-4 DNA was detected only in a nasal swab of one of the tested foetuses. Neither EHV-1 nor EHV-4 DNA was detected in the swabs of aborting mares. Sequence analysis of the glycoprotein B of the strains was performed and a phylogenetic tree was generated. The results indicated that 4 of the 5 Turkish EHV-1 strains (TR02, TR03, TR04 and TR05) clustered together; the fifth strain (TR01) was slightly removed from the group and clustered with other EHV-1 from various origins. Single nucleotide polyporphism (SNP in ORF30) associated with neuropathogenesis was not detected in any of the strains. At necropsy, sub-milier focal necrosis in the liver and spleen was observed. Microscopically, focal coagulation necrosis and marked eosinophilic intranuclear and intracytoplasmic inclusion bodies in the hepatocytes localised around the necrotic areas in the liver. Severe coagulation necrosis in white pulp of the spleen was also observed.  相似文献   

Investigation of serum amino acid and serum amyloid A concentrations in chickens with amyloid arthropathy     

Sevimli A  Yalcin M  Cangul IT  Aydin S 《The Veterinary quarterly》2012,32(1):17-23

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First Report on the Frequency and Molecular Detection of Neuropathogenic EHV-1 in Turkey     

Huseyin Yilmaz  Eda Altan  Nuri Turan  Aydin Gurel  Damla Haktanir  Kivilcim Sonmez  Sezgin Deniz  Ahmet Gulcubuk  Emre Gur  Gunes Sonmez  Juergen A. Richt 《Journal of Equine Veterinary Science》2012

There has been an increase in outbreaks of neuropathogenic equine herpesvirus-1 (EHV-1) in the United States and Europe. However, the presence and frequency of neuropathogenic EHV-1 in Turkish horses are not known at present. This study aimed to investigate the frequency of EHV-1 and neuropathogenic strains of EHV-1 in the Marmara Region of Turkey. Samples were analyzed for the presence of EHV-1 and neuropathogenic EHV-1 by real-time PCR TaqMan probe assays. Overall detection rate of EHV-1 was 45.5% (51 of 112). The detection rates were 70.5% (24 of 34) in aborted fetuses, 53.3% (8 of 15) in neonatal deads, 66.6% (4 of 6) in foals, 40% (2 of 5) in dead mares, and 25% (13 of 52) in living mares. Overall detection rate of neuropathogenic EHV-1 was 7.8% (4 of 51), and the real-time PCR results were confirmed by sequencing. Neuropathogenic strains of EHV-1 were detected in the brain and lung of two mares with neurological disease but without a history of abortion, in the brain of a foal that died of respiratory disorder, and in the nasal swab from a mare with a history of abortion. On histopathology, nonpurulent meningoencephalitis, hemorrhages, and vasculitis were seen in the brain. In conclusion, results of this study indicated, for the first time, that the neuropathogenic EHV-1 is circulating in the Marmara Region of Turkey. The results of this study also show that the current risk for non-neuropathogenic strains is high, whereas risk for the neuropathogenic EHV-1-G₂₂₅₄ strain seems to be low. As outbreaks of EHV-1 continue in the Marmara region of Turkey, surveillance for neuropathogenic EHV-1 genotype should be maintained.  相似文献   

Maintaining the carbon–oxygen balance in residential areas: A method proposal for land use planning     

Mediha Burcu Silaydin Aydin  Duygu Çukur 《Urban Forestry & Urban Greening》2012,11(1):87-94

The efforts to mitigate CO₂, one of the greenhouse gases causing global warming and climate change problems, have an important place in the worldwide agenda. One of the factors that cause CO₂ emissions is energy use in residential buildings, hence urban residential use is one of the areas on which mitigation efforts are focused. As land use planning determines the spatial development, density and form of residential areas, it has a direct role in the formation of residential emissions. However, these efforts to mitigate emissions could not yet be integrated with land use planning. It is possible to list the reasons as the mitigation efforts are made either on the building scale (mitigation of residential emissions by technological–architectural solutions) or on a global–regional scale (development of carbon sinks), the focus on transportation sector, and in land use planning, lack of considering the potential of urban green areas to mitigate emissions. By taking into consideration the O₂ production potential of green areas, a method proposal for land use planning is developed in this study to maintain the carbon–oxygen balance in residential areas. Depending on the form and density of the residential areas developed in a plan, the necessary size of green areas in such a plan is estimated by the method proposed. The varying emission amounts in different residential areas with diverse housing types, the number of actively used residential units, and local vegetation differences are included in the estimate. Therefore, an estimation method proposal that could be easily used in land use planning and that considers local differences is presented in the paper.  相似文献   

Use of topical selamectin for the treatment of Syphacia muris infection in laboratory rats     

Esatgil MU  Gülanber A  Aydin H 《Polish journal of veterinary sciences》2008,11(1):67-69

Efficacy of selamectin was studied in naturally acquired S. muris infections in rats. Fourty-eight S. muris-positive rats were divided into six treated and two control groups. Selamectin (6 mg/kg) was applied topically to the skin in a single spot at the base of the neck in the treatment group. The rats of treated and control groups were necropsied on the 24th day after the treatment. Topical selamectin was found to be 40.7-63.3% effective (based on egg per gram method) in eliminating S. muris infection in rats. The efficacy of the treatment against S. muris (based on adult worm counts) in male and female rats was 35.14-58.88%, respectively (mean 48.39%).  相似文献