Antioxidant and stomatal responses of grapevine (Vitis vinifera L.) to boron toxicity     

A. Gunes  G. Soylemezoglu  A. Inal  E.G. Bagci  S. Coban  O. Sahin 《Scientia Horticulturae》2006

Although of considerable agronomic importance, our understanding of B toxicity mechanism in plants is still not completely understood, and remains an open question. Therefore, we investigated the effect of increasing levels of B (0, 10, 20 and 30 mg kg⁻¹) on the growth, boron (B) concentrations, stomatal resistance, lipid peroxidation (MDA), membrane permeability (MP), lypoxygenase activity (LOX), proline (PRO) and H₂O₂ accumulation, and the activities of major antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT and ascorbate peroxidase, APX) of grapevine (Vitis vinifera L. cv. Kalecik Karasi) grafted on 5BB rootstock (V. berlandieri × V. riparia) was investigated. Applied toxic levels of B significantly reduced leaf and root growth and increased the B concentration of the leaf, and stem, bark and root of rootstock. In the all B levels leaf tissues of grapevine accumulated more B than that of the other plant parts. In order to restrict excessive uptake of B, stomatal resistance of the leaves increased especially at high B treatments (20 and 30 mg kg⁻¹). The concentrations of H₂O₂, MDA and membrane permeability were increased as the result of B toxicity while proline and the activity of lypoxygenase were decreased. Compared with control plants, the activities of SOD and CAT were increased by B treatments while the activity of APX was decreased. To our knowledge, this is the first report that B toxicity elevated the antioxidant enzymes to protect the membrane functions from reactive oxygen species (ROS) injury in grapevine and it was hoped that this study would provide a basis for developing strategies for reducing the risks associated with B toxicity.  相似文献   

Changes in antioxidative system and membrane damage of lettuce in response to salinity and boron toxicity     

F. Eraslan  A. InalO. Savasturk  A. Gunes 《Scientia Horticulturae》2007

Individual and combined effects of salinity and B toxicity on growth, the major antioxidant enzymes (superoxide dismutase, SOD; catalase, CAT; ascorbate peroxidase, APX) activities, ascorbic acid, proline, and H₂O₂ accumulation, and stomatal resistance (SR), malondialdehyde (MDA), membrane permeability (MP) and the concentrations of sodium (Na), chloride (Cl) and boron (B) of lettuce were investigated. Boron toxicity and salinity reduced growth of lettuce plants. Under B toxicity, B concentration of the plants was increased, but in the presence of NaCl, the concentration of B was significantly reduced. Sodium and Cl concentrations were increased in B + NaCl and NaCl treatments. Membrane damage was more pronounced in NaCl and B + NaCl treatments. Stomatal resistance of the plants was significantly increased by salinity treatments. The accumulation of proline and ascorbic acid was the highest in the B + NaCl treatment. In general, stress conditions significantly increased H₂O₂ and antioxidant enzyme (SOD, CAT and APX) activities. The present results indicate that stomatal closure is an important response of lettuce against NaCl and B + NaCl stress. Furthermore NaCl and B + NaCl toxicity-induced oxidative stress in lettuce resulting in lipid peroxidation and membrane damage. Increased antioxidant enzyme activities and also accumulation of ascorbic acid and proline are involved in order to overcome B- and NaCl-induced oxidative stress.  相似文献   

A retrospective study of gastrointestinal disorders in a predominantly Austrian leisure horse referral hospital population     

V. Gunes  A. C. Onmaz  A. Pavaloiu  G. Kaya Karasu  R. van den Hoven 《Equine Veterinary Education》2022,34(9):467-472

The aim of our retrospective study was to produce and analyse epidemiological data on gastrointestinal (GI) disorders of Austrian leisure horses. Case records of horses referred to the Equine Clinic of the Veterinary University of Vienna were collected from an electronic hospital information system using its search options. All cases reported to have suffered a GI disorder between March 2003 and February 2015 were selected. In this period of 4314 days, data of 1833 GI cases, mostly accompanied by colic signs, were analysed. The vast majority of our GI cases appeared in the abdominal parts of the GI tract 96% (1760/1833) while just 1.3% (24/1833) could be definitively attributed to oesophageal disorders, whereas in 2.7% (49/1833) of the cases a definitive diagnosis could not be made. The highest prevalence of GI disorders was seen in July and October/November. A breakdown of figures for cases clustered under the colic syndrome showed that colon impactions in left ventral large and/or pelvic flexure (35%) were the major problem followed by gastric disorders (18%), spasmodic colic (9%), enteritis (7%), caecal tympany (6%); caecal impaction (4%), colitis (3%), abnormal anatomical positions of large colon including complete and partial strangulation/volvulus, nephrosplenic entrapment of the left ventral and dorsal colon and right dorsal displacement of the large colon (3%) and small intestinal volvulus (2.7%). In 12% of the cases, the cause of the abdominal discomfort was likely idiopathic paralytic ileus. The searching tool of the electronic patient data also brought up cases with malabsorbtion/IBD syndrome and GI ruptures, both these contributed less than 1% of the referred GI cases. Results suggested that the causes of colic are diverse, but the major reasons for referred cases of colic in the east Austrian horse population are large colon impaction or displacements. Seasonal distribution and specific causes of gastrointestinal disorders should be considered essential components of the initial clinical assessment of horses presenting with colic.  相似文献   

Genetic analysis of selected almond genotypes and cultivars grown in Turkey using peroxidase-gene-based markers     

Hasan Pinar  Mustafa Unlu  Sezai Ercisli  Aydin Uzun  Mustafa Bircan 《林业研究》2016,27(4):747-754

Peroxidase plays important roles in many stress-related interactions and catalyzes important reactions in various physiological processes. Since peroxidase played critical roles in the evolution of almond(Prunus dulcis Miller(D.A Webb) syn P. amygdalus Batsch), peroxidase-gene-based analyses may increase the understanding of evolution of this species. Peroxidase gene polymorphism(POGP) markers were used to determine genetic diversity and relationships among 69 Turkish genotypes/cultivars and 27 foreign almond cultivars by using unweighted pair group method with arithmetic mean(UPGMA) analysis. This study is the first evaluation of the use of POGP markers for diversity analysis in almond.Totally, 83 fragments were obtained from eight peroxidase primer pairs, and polymorphism was identified as 94 %.Similarity level among the genotypes ranged between 0.63 and 0.93, and all materials were distinguished. In general,Turkish and foreign genotypes were mixed in clusters since they share a common genetic background and gene migration among the sites. Clusters were not based on geographic regions except for some minor groupings. This study indicated that peroxidase gene markers can be reliably used to determine genetic relationships in almonds.  相似文献   

Optimization of a profile extrusion die for flow balance     

Oktay Yilmaz  Hasan Gunes  Kadir Kirkkopru 《Fibers and Polymers》2014,15(4):753-761

One of the problems encountered in the extrusion of plastic profiles is unbalanced flow at the die exit. It causes deformation of the extrudates at ambient and precludes the material transition through remaining stages of production process (calibration, cooling sections etc). In this paper, geometric parameters of a profile extrusion die are optimized using several objective function definitions by Simulated Annealing-Kriging Meta-Algorithm. Objective functions are defined based on the uniformity of velocity distribution at the die exit. For this, Computational Fluid Dynamics (CFD) simulations are performed for N=70 die geometries. Appropriate geometric parameters (t and L) of the die are variables for the optimization problem.  相似文献   

The effect of various nitrogen fertilizers on zinc (zn) availability in non calcic brown forest soils     

Aydin Adiloglu  Sevinç Adiloglu  Esin Gonulsuz  Nureddin Oner 《Archives of Agronomy and Soil Science》2013,59(6):601-606

In this article the effect of different nitrogen fertilizer application on the availability of zinc (Zn) content in non calcic brown forest soil was investigated. For this purpose increasing rates of NH₄Cl, (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 fertilizers were applied for having different physical and chemical properties in two soil samples. An incubation experiment was carried out over 4 months. The available Zn content of the soils was determined each month. According to the results the highest available Zn content of the soils was obtained from NH₄Cl applied to the soils followed by (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 application to soil. At the end of the experiment the average available Zn content of non calcic brown forest soils for NH₄Cl, (NH₄)₂S0₄; NH₄N0₃ and Ca(N0₃)₂.4H₂0 fertilizers are 0.93, 0.91, 0.88 and 0.77 mg kg^?1, respectively.  相似文献   

Effect of Water Stress at Various Growth Stages on Some Quality Characteristics of Winter Wheat   总被引：8，自引：0，他引：8  

A. Ozturk  F. Aydin 《Journal of Agronomy and Crop Science》2004,190(2):93-99

A field experiment was carried out in Erzurum (Turkey) on winter wheat to analyse the effect of water stress at different growth stages – fully irrigated (FI), rainfed (R), early water stress (EWS), late water stress (LWS) and continuous water stress (CWS), on some quality characteristics – in the 1995–96 and 1996–97 cropping seasons. Water stress had a substantial effect on most of the quality characteristics. As averages of cropping seasons, CWS, EWS, R and LWS treatments decreased grain yields by 65.5, 40.6, 30.5 and 24.0 %, respectively, compared with the FI treatment. CWS increased grain protein content by 18.1 %, sedimentation volume by 16.5 %, wet gluten content by 21.9 % and decreased 1000‐kernel weight by 7.5 g compared with FI treatment. LWS caused an increase of 8.3 % in grain protein content, 8.7 % in sedimentation volume, 10.8 % in wet gluten content and a reduction of 3.8 g in 1000‐kernel weight compared with FI. EWS and R increased sedimentation volume and wet gluten content, but decreased 1000‐kernel weight compared with FI. The effect of LWS on grain quality was more significant than that of EWS. The results suggest that soil moisture conditions increase grain yield and kernel weight of winter wheat but decrease its quality.  相似文献   

Determination of suitable chemical extraction methods for the available iron content of brown forest soils in Turkey     

Aydin Adiloglu 《Eurasian Soil Science》2006,39(9):961-967

The aim of this research was to determine the available iron (Fe) content of brown forest soils of Edirne Province and the most suitable chemical extraction method. Eight chemical extraction methods (the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 MTEA, 0.05 M HCl + 0.012 M H2SO4, 1 M NH4OAc (pH: 4.8), 0.01 M EDTA + 1 M NH4OAc, 1 M MgCl2, 0.01 M EDTA + 1 M (NH4)2CO3, 0.005 M DTPA + 1 M NH4HCO3, and 0.001 M EDDHA methods) and six biological indices (the dry matter yield, Fe concentration, Fe uptake, relative dry matter yield, relative Fe concentration, and relative Fe uptake) were compared. The biological indices were determined with barley (Hordeum vulgare L.) grown under greenhouse conditions. At the end of the experiment, the highest correlation coefficients (r) were determined to be between the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA method and the biological indices and between the 0.005 M DTPA + 1 M NH4HCO3 method and the biological indices. The corresponding correlation coefficients (r) for the 0.005 M DTPA + 0.01 M CaCl2 + 0.1 M TEA method and the six biological indices were 0.621**, 0.823**, 0.810** 0.433**, 0.558**, and 0.640**, respectively. For the 0.005 M DTPA + 1 M NH4HCO3 method, these coefficients were equal to 0.618**, 0.520**, 0.679**, 0.521**, 0.492**, and 0.641**, respectively (** indicate the validity of the relationships at p < 0.01) These extraction methods, out of all the methods tested, were suggested for the determination of the available Fe content of the brown forest soils. Published in Russian in Pochvovedenie, 2006, No. 9, pp. 1068–1074. The text was submitted by the author in English.  相似文献   

Genotypic variation in phosphorus efficiency between wheat cultivars grown under greenhouse and field conditions     

Aydin GUNES  Ali INAL  Mehmet ALPASLAN  Ismail CAKMAK 《Soil Science and Plant Nutrition》2006,52(4):470-478

  相似文献   

Effect of time delay and storage temperature on blood gas and acid-base values of bovine venous blood     

Gokce G  Citil M  Gunes V  Atalan G 《Research in veterinary science》2004,76(2):121-127

The aim of this study was to investigate possible changes in the gas composition and acid-base values of bovine venous blood samples stored at different temperatures (+4, 22 and 37 degrees C) for up to 48 h. Five healthy cattle were used in the study. A total of 15 blood samples collected from the animals were allocated into three groups, which were, respectively, then stored in a refrigerator adjusted to +4 degrees C (Group I, n=5), at a room temperature of about 22 degrees C (Group II, n=5) and in an incubator adjusted to 37 degrees C (Group III; n=5) for up to 48 h. Blood gas and acid-base values were analysed at 0 (baseline), 1, 2, 3, 4, 5, 6, 12, 24, 36 and 48 h of storage. A significant decrease (p<0.001) was found, in the pH of the refrigerated blood after 5 h and its maximum decrease was recorded at 48 h as 0.04 unit. There were also significant alterations (p<0.001) in the blood pH of the samples stored at room temperature and in the incubator after 2 and 3 h, respectively. The maximum mean alteration in pCO(2) value for Group I was -0.72 kPa during the assessment, while for groups II and III, maximum alterations in pCO(2) were detected as +2.68 and +4.16 kPa, respectively. Mean pO(2) values increased significantly (p<0.001) for Group I after 24 h and for Group II after 6 h, while a significant decrease was recorded for Group III after 24 h (p<0.001). Base excess (BE) and bicarbonate (HCO(3)) fractions decreased significantly for all the groups during the study, compared to their baseline values. In conclusion, acid-base values of the samples stored at 22 and +4 degrees C were found to be within normal range and could be used for clinical purposes for up to 12 and 48 h, respectively, although there were small statistically significant alterations.  相似文献