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Noli C Carta G Cordeddu L Melis MP Murru E Banni S 《Veterinary journal (London, England : 1997)》2007,173(2):413-421
Although conjugated linoleic acid (CLA) shows inhibitory effects on histamine release, eicosanoid production and pruritus in laboratory rodents, its use in canine atopic dermatitis (AD) has not been reported. The aims of this study were to assess the efficacy of CLA, black currant seed oil (BSO) or a combination of both, compared to placebo, in dogs with AD and to evaluate any changes in fatty acid metabolism with these treatments. Twenty-four dogs with AD were randomly allocated to four groups, and were treated orally each day for two months with either 1 mL/10 kg CLA (80% purity), 1 mL/10 kg pure BSO, 1 mL/10 kg CLA+1 mL/10 kg BSO, or 1 mL/10 kg sugar syrup (placebo). Serum was obtained on days 0, 30 and 60 for analysis of CLA metabolites, linoleic acid (LA), gamma-linolenic acid (GLA), dihomo-gamma-linolenic acid (DGLA) and arachidonic acid (AA). At the same time point, the owners were asked to assess pruritus and the veterinarians evaluated any skin lesions present. Although the best clinical results occurred with BSO treatment alone, improvement of clinical signs and pruritus was not significant with any of the treatments. Serum levels of GLA and DGLA significantly increased in BSO-treated dogs, but not in the CLA+BSO group. CLA at the dosage used in this study was not efficacious in treating canine AD, whereas BSO may help some dogs with AD, although further studies are necessary before this can be recommended as a treatment. 相似文献
2.
J. Ghedira J. Jebali Z. Bouraoui M. Banni H. Guerbej H. Boussetta 《Fish physiology and biochemistry》2010,36(1):101-107
The levels of metallothionein (MT), a biomarker of metal exposure, and of Cd and Cu, known as MT inducers, were investigated
in Sparus aurata intraperitoneally injected with 500 μg/kg of Cu and Cd for 2 days. MT and metal concentrations (Cd and Cu) were determined
in liver, gills and kidney. MT levels were significantly increased in all investigated tissues, with the highest value in
liver of Cu as Cd-treated fishes (3.56-fold and 3.3- fold, respectively). Metal concentrations were statistically different
between all tissues. Highest metal concentrations were in the liver. The higher metal concentrations and MT induction levels
support the main role of MT in metal homeostasis and detoxification. 相似文献
3.
Boughattas Iteb Hattab Sabrine Alphonse Vanessa Livet Alexandre Giusti-Miller Stéphanie Boussetta Hamadi Banni Mohamed Bousserrhine Noureddine 《Journal of Soils and Sediments》2019,19(1):296-309
Purpose
A better understanding is required of the potential of soil biota in controlling the availability and mobility of heavy metals and ascertaining their toxicity. The objectives of this work are to assess, first, the modification of heavy metal speciation induced by earthworms Eisenia andrei and, second, the consequence of this metal speciation change on soil enzyme activities as an easy bioindicator of stress.
Materials and methodsThe experiment was conducted on six sites from Jebel Ressas Mines, which are characterized by a gradient heavy metal contamination (Pb, Zn, and Cd). Earthworms E. andrei were introduced in these six soils for 60 days. We had performed heavy metal speciation both in the presence and absence of worms. Modifications of activities of seven enzymes implicated in C, N, and P biochemical cycles were used as a bioindicator of metal stress. We had used the co-inertia statistical method to evaluate the correlation between change in heavy metal speciation induced by earthworms and the enzyme activities in soils.
Results and discussionOur results suggested that earthworms modified the heavy metal dynamic and speciation. They decrease the amount of metal associated with the most available fraction, such as exchangeable one, and increase the amount of metal bound to the more stable fraction, like Mn and Fe oxide ones. On the same hand, enzyme activities increased in majority of the soils, following earthworm activity, but this effect is dependent on the amount of soil contamination. Moreover, the co-inertia results denote that change in heavy metal speciation significantly influences the soil enzyme activities in Jebel Ressas soils, especially β-glucosidase, urease, deshydrogenase, and fluorescein diacetate hydrolysis (FDA), and can be considered as bioindicators of metal toxicity and biological quality in the contaminated area.
ConclusionsBy reducing the availability of heavy metals, the earthworms are useful in the bioremediation of heavy metal contaminated soils. Soil enzymes β- glucosidase, urease, deshydrogenase, and FDA can be used to assess the changes in metal speciation and can let us, therefore, predict if the soils are bioremediated.
相似文献4.
M. Banni Z. Bouraoui J. Ghedira C. Clerandeau H. Guerbej J. F. Narbonne H. Boussetta 《Fish physiology and biochemistry》2009,35(2):293-299
In the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons
benzo[a]pyrene (B[a]P) were investigated in the liver of Sparus aurata (sea bream). Sexually immature gilthead sea bream were treated by intraperitoneal injection of B[a]P (20 mg kg−1) for 6, 12, 24, and 48 h. B[a]P accumulation was quantified in sea bream liver by mean of gas phase chromatography (GPC-MS)
after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity, as a phase I biotransformation parameter; (2) liver glutathione S-transferase (GST) activity as a phase II conjugation enzyme. DNA damage was assessed over time using the single-cell gel
electrophoresis comet assay. B[a]P bioaccumulation in the liver resulted in a biphasic curve with an increasing uptake up
to 5.55 ± 0.67 μg g−1 dry weight after only 6 h exposure and 4.67 ± 0.68 μg g−1 dry weight after 48 h exposure. EROD activity showed a nonsymmetrical bell-shaped kinetic with a maximum at 24 h and lower
but significant activities at 12 and 48 h with respect to control animals. Hepatic GST activities were only significant after
48 h exposure. Comet assay showed an increase in liver cells DNA damage with a maximum after 48 h exposure reaching up to
12.17 %DNA in the tail. 相似文献
5.
Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata 总被引：1，自引：1，他引：0
Bouraoui Z Banni M Ghedira J Clerandeau C Guerbej H Narbonne JF Boussetta H 《Fish physiology and biochemistry》2008,34(3):201-207
This research was designed to study Sparus aurata (sea bream) biotransformation and detoxification responses to acute exposure to cadmium (Cd). Sexually immature gilthead
sea bream were treated by intraperitoneal injection of Cd chloride (200 μg kg−1) for 6, 12, 24 and 48 h. Cd accumulation was quantified in sea bream liver by graphite furnace atomic absorption spectroscopy
after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD)
activity as phase I biotransformation parameter, (2) liver glutathione-S-transferase (GST) activity as a phase II conjugation
enzyme and metallothionein (MT) content as specific response to Cd contamination. Cd bioaccumulation in the liver resulted
in an increasing uptake up to 10.3 μg g−1 wet weight after 48 h of exposure. EROD showed a significant activation only after 6 h exposure and a return to control levels
after 12 h. GST revealed significant activation starting from 12 h exposure. MT accumulation in liver showed the same behavior
as GST activation. 相似文献
6.
The purpose of this study was to evaluate a combination of immunostimulatory bacterial DNA sequences and allergen-specific immunotherapy for the treatment of canine atopic dermatitis. Seven dogs with nonseasonal atopic dermatitis diagnosed by history, clinical signs and exclusion of differential diagnoses were included. All dogs had been on allergen-specific immunotherapy for at least 12 months with incomplete responses, were on additional antipruritic therapy and showed residual pruritus. Pruritus was marked by the owner on a visual analogue scale, lesions were determined by a clinician using the Canine Atopic Dermatitis Extent and Severity Index (CADESI), and concurrent medications were recorded before entering the study and after 14 weeks of treatment. Peripheral blood mononuclear cells were isolated and cultured; canine cytokine message for IFNγ, IL-4, TNF and IL-10 was quantitated using RT-PCR. A mixture of allergen extract and liposome-DNA complexes was injected intradermally at the beginning of the study and after 2, 4, 6, 10 and 14 weeks. CADESI, pruritus and medication scores, and cytokine messages at the beginning and end of the study were compared with a paired t -test. There were significant improvements in pruritus scores ( P = 0.0277). Reductions in medication scores and CADESI were not statistically significant. IL-4 production decreased significantly ( P = 0.0428); decreases in other cytokines were not significant. Although the number of dogs in this pilot study was small, the results warrant further investigation of a combination of immunostimulatory bacterial DNA sequences and allergen-specific immunotherapy for the treatment of canine atopic dermatitis.
Funding: Self-funded. 相似文献
Funding: Self-funded. 相似文献
7.
Hattab Sabrine Hedheli Afif Banni Mohamed Boussetta Hamadi Herrero Maria 《Scientia Horticulturae》2010
In this work, pea (Pisum sativum) plants exposed to increasing cadmium and copper concentrations were tested for heavy metals accumulation in flowers and for ‘in vivo’ pollen germination. Based on the Cd and Cu accumulation amounts in the flowers, an evaluation of the same metals effects on ‘in vitro’ pollen germination was achieved. Moreover, the effects of both metals on fruits number and weight and on seed set and yield at individual plant level were examined. While cadmium concentrations did not affect ‘in vivo’ pollen germination, only higher copper concentrations rendered a significant reduction. This is in contrast with the clear negative effect on pollen germination in vitro and might be explained by the different dynamic and bioavailability of both metals. A clear effect of Cd and Cu was observed on two important yield components ie, fruit weight and seed set. Although results obtained herein cannot give a clear cut relationship between the effect of Ca and Cu on reproductive development and its consequences on yields, they represent emerging results on the potential consequences of metals contamination on reproductive development in plants. 相似文献
8.
J. Jebali M. Banni H. Guerbej E. A. Almeida A. Bannaoui H. Boussetta 《Fish physiology and biochemistry》2006,32(1):93-98
The potential use of acetylcholinesterase (AChE) and metallothionein (MT) responses as biomarker of organophosphorous (OPs)
and trace metal were assessed in fish Seriola dumerilli exposed to 0, 4, 6 mg/kg of malathion for 2, 7 and 13 days, and to 0, 50, 100, 250 μg/kg of Cd for 2 days. Brain AChE was
significantly inhibited after 2 and 7 days of malathion exposure, in a dose–response manner, but no inhibition was observed
after 13 days of exposure. When exposed to Cd for 2 days, S. dumerelli presented an increase in AChE activity at a concentration of 50 μg/kg, but a strong and dose-dependent AChE inhibition at
100 and 250 μg/kg. Cd treatment also caused a rapid increase in MTs concentration in liver, even at the lower concentration.
Our experiments indicate that the measurement of hepatic MT concentration and brain AChE activity in S. dumerilli would be useful biomarkers of OP and Cd exposure and/or effects. 相似文献
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