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BACKGROUND: The present study investigated the effect of seven Bacillus‐species plant‐growth‐promoting rhizobacteria (PGPR) seed treatments on the induction of disease resistance in cowpea against mosaic disease caused by the blackeye cowpea mosaic strain of bean common mosaic virus (BCMV). RESULTS: Initially, although all PGPR strains recorded significant enhancement of seed germination and seedling vigour, GBO3 and T4 strains were very promising. In general, all strains gave reduced BCMV incidence compared with the non‐bacterised control, both under screen‐house and under field conditions. Cowpea seeds treated with Bacillus pumilus (T4) and Bacillus subtilis (GBO3) strains offered protection of 42 and 41% against BCMV under screen‐house conditions. Under field conditions, strain GBO3 offered 34% protection against BCMV. The protection offered by PGPR strains against BCMV was evaluated by indirect enzyme‐linked immunosorbent assay (ELISA), with lowest immunoreactive values recorded in cowpea seeds treated with strains GBO3 and T4 in comparison with the non‐bacterised control. In addition, it was observed that strain combination worked better in inducing resistance than individual strains. Cowpea seeds treated with a combination of strains GBO3 + T4 registered the highest protection against BCMV. CONCLUSION: PGPR strains were effective in protecting cowpea plants against BCMV under both screen‐house and field conditions by inducing resistance against the virus. Thus, it is proposed that PGPR strains, particularly GBO3, could be potential inducers against BCMV and growth enhancers in cowpea. Copyright © 2009 Society of Chemical Industry  相似文献   
2.
Enrichment of soil organic carbon (SOC) stocks through sequestration of atmospheric CO2 in agricultural soils is important because of its impacts on adaptation to and mitigation of climate change while also improving crop productivity and sustainability. In a long‐term fertility experiment carried out over 27 y under semiarid climatic condition, we evaluated the impact of crop‐residue C inputs through rainfed fingermillet (Eleusine coracana [L.] Gaertn.) cropping, fertilization, and manuring on crop yield sustainability and SOC sequestration in a Alfisol soil profile up to a depth of 1 m and also derived the critical value of C inputs for maintenance of SOC. Five treatments, viz., control, farmyard manure (FYM) 10 Mg ha–1, recommended dose of NPK (50 : 50 : 25 kg N, P2O5, K2O ha–1), FYM 10 Mg ha–1 + 50% recommended dose of NPK, and FYM 10 Mg ha–1 + 100% recommended dose of NPK imposed in a randomized block design replicated four times. Application of FYM alone or together with mineral fertilizer resulted in a higher C input and consequently built up a higher C stock. After 27 y, higher profile SOC stock (85.7 Mg ha–1), C build up (35.0%), and C sequestration (15.4 Mg C ha–1) was observed with the application of 10 Mg FYM ha–1 along with recommended dose of mineral fertilizer and these were positively correlated with cumulative C input and well reflected in sustainable yield index (SYI). For sustenance of SOC level (zero change due to cropping) a minimum quantity of 1.13 Mg C is required to be added per hectare per annum as inputs. While the control lost C, the application of mineral fertilizer served to maintain the priori C stock. Thus, the application of FYM increased the C stock, an effect which was even enhanced by additional amendment of mineral fertilizer. We conclude that organic amendments contribute to C sequestration counteracting climate change and at the same time improve soil fertility in the semiarid regions of India resulting in higher and more stable yields.  相似文献   
3.
A long-term experiment was conducted to evaluate the effect of integrated use of organic and inorganic sources of nutrients on soil quality and its relation to finger millet yield under two predominant crop rotations viz., groundnut–finger millet and finger millet monocropping in hot moist semiarid rainfed Alfisol soils in South India. Two experiments were laid out separately for each cropping system in a randomized complete block design with five treatments individually with FYM and maize residue-based combinations viz., Control (T1), FYM @ 10t ha ?1 or Maize residue (MR) @ 5t ha ?1 (T2), farm yard manure (FYM) @ 10t ha ?1 or Maize residue (MR) @ 5t ha ?1 + 50% RDF (Recommended Fertiliser Dose) N, P2O5 &; K2O (T3), FYM @ 10t ha ?1 or Maize residue (MR) @ 5t ha ?1 + 100% RDF N,P2O5 &; K2O (T4), Recommended N, P2O5 &; K2O (T5). Thus, four sets of nutrient management systems were evaluated. The results showed that farm yard manure or maize residue application in combination with recommended dose of fertilizer significantly improved the soil physical, chemical, and biological properties compared to control and application of inorganic fertilizers alone. Based on evaluation of 19 soil quality parameters under each of the four nutrient management systems, the common key soil quality indicators emerged out were: organic C (OC), available nitrogen (N), available sulfur (S), and mean weight diameter (MWD) of soil aggregates. A significant correlation between the finger millet yield and the relative soil quality indices (RSQI) indicates the importance of soil quality in these semiarid Alfisol soils. The results and the methodology adopted in the present study could be of importance in improving the soil quality not only for the region of the study, but also in other identical soils and cropping systems across the world.  相似文献   
4.
Field experiments were conducted to assess changes in soil infiltration, water-holding capacity (WHC), bulk density and soil fertility with 15 custard apple (CA)-based systems of T1:CA+Fingermillet; T2:CA+Fodder Maize; T3:CA+Fieldbean; T4:CA+Niger; T5:CA+Chilli; T6:CA+Cowpea; T7:CA+Foxtailmillet; T8:Fingermillet; T9:Fodder Maize; T10:Fieldbean; T11:Niger; T12:Chilli; T13:Cowpea; T14:Foxtailmillet and T15:CA in 2012 and 2013 under semiarid Alfisols at Bangalore. T2 gave the maximum CA equivalent yield (1333 kgha?1), rainwater-use efficiency (4.90 kgha?1mm?1), net returns of Rs. 52690 ha?1 and a benefit-cost ratio of 2.92. T6 gave the maximum WHC of 41.0%; an infiltration rate of 33.1 cmhr?1; a cumulative infiltration of 66.2 cm; and a minimum bulk density of 1.40 gcc?1. T6 maintained maximum soil nitrogen (N) of 259 kgha?1; phosphorus pentoxide (P2O5) of 67.9 kgha?1; and potassium oxide (K2O) of 141.5 kgha ?1; while T9 gave uptake N of 102.7 kgha?1; P2O5 of 24.6 kgha?1; and K2O of 103.7 kgha?1. T6 was efficient for improving the soil physical, chemical, and biological parameters based on the study.  相似文献   
5.
A field experiment was conducted during the summer of 1996 at the Main Research Station, University of Agricultural Sciences, Hebbal, Bangalore to study the effect of phenophased irrigation schedules on green cob and fodder yield and to delineate critical stages based on Stress Day Index. There were seven irrigation schedules consisting of a combination of two IW/CPE ratios (irrigation water/cumulative pan evaporation), viz. 0.6 and 0.8 shuffled at three growth stages: 10–35 DAS (days after sowing), 36–65 DAS and 66 days to harvest in RCBD (randomized complete block design) with four replications. The investigations revealed that all yield attributing characters such as cobs per plant, cob weight, green cob and fodder yields were significantly higher under the irrigation schedule of 0.8 IW/CPE ratio throughout the crop growth. The reduction in green cob and fodder yields were 39 and 25 % respectively, when irrigations were scheduled at 0.6 IW/CPE ratio throughout as compared to 0.8 IW/CPE. Also, frequent irrigation at 0.8 IW/CPE ratio throughout maintained higher plant relative water content (86.90 %) and higher available soil moisture (59.95–81.51 %) before irrigation as compared to delayed irrigations at 0.6 IW/CPE ratio. The quality parameter viz. sucrose content (2900 μg g−1) in developed seeds was also higher under frequently irrigated treatment. The stress day index indicated that vegetative followed by silking and tasseling were the critical stages for moisture stress.  相似文献   
6.
In a 20‐yr‐old long‐term experiment, the impact of continuous application of organic manures and inorganic fertilizers on soil quality and the sustainability of finger millet production was conducted on two cropping systems: finger millet and finger millet–groundnut on an Alfisol of semi‐arid southern India. The study was conducted from 1992 to 2011 at the All India Coordinated Research Project for Dryland Agriculture, UAS, Bangalore, using a randomized block design. The treatments comprised of T1: control [no fertilizer and no farmyard manure (FYM) applied], T2: FYM 10 t/ha, T3: FYM 10 t/ha + 50% of recommended NPK (50:50:25 kg/ha), T4: FYM 10 t/ha + 100% of recommended NPK and T5: 100% recommended NPK. Comparison of long‐term yield data between treatments was used to calculate a ‘sustainability yield index’ (SYI), which was greatest for T4 (FYM 10 t/ha + 100% of recommended NPK), in both rotational (0.68) and monocropping (0.63) situations. Soil quality indices were determined using principal component analysis linear scoring functions. The key indicators which contributed to the soil quality index (SQI) under rotation were organic C; potentially available N; extractable P, K and S; exchangeable Ca and Mg; dehydrogenase activity and microbial biomass C and N. The largest SQI (7.29) was observed in T4 (FYM 10 t/ha + 100% NPK), and the smallest (3.70) SQI was for the control. Application of 10 t/ha FYM together with NPK (50:50:25 kg/ha) sustained a mean yield of 3884 kg/ha.  相似文献   
7.
Field experiments were conducted in farmyard manure (FYM) and maize-residue (MR) blocks during 1984–2011 with the objective of identifying a superior treatment for attaining maximum finger millet yield and soil fertility at Bangalore. The treatments tested in the FYM block were Control; FYM@10tha?1; FYM@10tha?1 + 50% nitrogen, phosphorus, and potassium (NPK); FYM@10tha?1 + 100%NPK; 100%NPK; and in the MR block, Control; MR@5tha?1; MR@5t ha?1 + 50%NPK; MR@5tha?1 + 100%NPK; 100%NPK. FYM@10t/ha + 100%NPK gave a maximum mean yield of 3207 kg/ha in the FYM block, while MR@5t/ha + 100%NPK gave 2548 kg/ha in the MR block. Regression and principal component (PC) models of yield were developed through soil fertility and rainfall variables to assess the treatments. Maximum yield predictability of 60% and 65% under regression, and 76% and 75% under the PC model were observed for the FYM and MR blocks, respectively. FYM@10t/ha + 50%NPK was superior, with maximum gross returns of Rs.41286/ha and benefit–cost ratio (BCR) of 2.27 in the FYM block compared to MR@5t/ha + 100%NPK (gross returns of Rs.34530/ha and BCR of 2.09) in the MR block with maximum soil fertility, and are recommended for adoption under semi-arid Alfisols.  相似文献   
8.
Based on a long-term finger millet-groundnut rotation study conducted for 24-years during 1992–2015 under Alfisols at Bangalore, organic and inorganic fertilizer effects on soil organic carbon (SOC) sequestration and sustainability of yield were assessed. Field experiments were conducted with T1:Control; T2:FYM@ 10t ha?1; T3:FYM@ 10t ha?1 + 50% NPK; T4:FYM@ 10t ha?1 + 100% NPK, and T5:100% recommended NPK in same plot every year. T5 comprised of 50 kg N, 50 kg P2O5 and 25 kg K2O ha?1 for finger millet and 25 kg N, 50 kg P2O5 and 25 kg K2O ha?1 for groundnut. Sustainability yield index of treatments was assessed using measurements made on variability of yield over years. The amount of carbon sequestered was assessed to identify a superior treatment for improving soil quality. Balanced use of 100% NPK+ FYM for maintenance of SOC at antecedent level with biomass-C of 1.62 Mg C ha?1 year?1 was feasible for sustaining production under semi-arid Alfisols.  相似文献   
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