Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)          下载免费PDF全文

DSR Angrimani  KK Nagai  BR Rui  LC Bicudo  JDA Losano  MM Brito  MCP Francischini  M Nichi 《Reproduction in domestic animals》2018,53(1):163-170

Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer‐assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3′3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.  相似文献   

A fast,low‐cost and efficient method for the diagnosis of sperm DNA fragmentation in several species          下载免费PDF全文

BR Rui  DSR Angrimani  LC Bicudo  JDA Losano  M Nichi  RJG Pereira 《Reproduction in domestic animals》2018,53(1):171-175

Sperm DNA fragmentation is a condition that interferes directly in the reproductive efficiency. Currently, there are several methods for assessing the sperm DNA integrity, such as Alkaline Comet, TUNEL and Sperm Chromatin Structure Assay. However, many of these techniques are laborious and require high‐precision equipment. Thus, the development of new techniques can optimize the evaluation of sperm DNA damage. Therefore, the aim of this study was to standardize the toluidine blue (TB) stain technique for the analysis of DNA fragmentation of dog, cat, bull, stallion and ram spermatozoa. For this purpose, we used six animals of each specie (n = 30), in reproductive age. Sperm was collected by different methods according to the particularities of each species, and such samples were divided into two aliquots: a sperm sample was kept at 5°C (considered as intact sperm DNA), and the remaining samples were submitted to the induction of DNA fragmentation by exposure to ultraviolet light for 4 hr. Samples were then mixed with the intact sample to obtain known and progressive proportions of sperm with fragmented DNA (0%, 25%, 50%, 75% and 100%). Semen smears were performed and subjected to staining with TB. Blue‐stained spermatozoa were considered to have DNA fragmentation. We observed high linear regression coefficients between the expected proportion of damaged DNA and the results of TB for dog, cat, ram, bull and stallion samples. In conclusion, TB stain was considered a fast and effective technique for the study of spermatozoa DNA in several species.  相似文献   

Antioxidant effectiveness of phenolic apple juice extracts and their gut fermentation products in the human colon carcinoma cell line caco-2     

Bellion P  Hofmann T  Pool-Zobel BL  Will F  Dietrich H  Knaup B  Richling E  Baum M  Eisenbrand G  Janzowski C 《Journal of agricultural and food chemistry》2008,56(15):6310-6317

Apples represent a major dietary source of antioxidative polyphenols. Their metabolic conversion by the gut microflora might generate products that protect the intestine against oxidative damage. We studied the antioxidant effectiveness of supernatants of fermented apple juice extracts (F-AEs, 6 and 24 h fermentation) and of selected phenolic degradation products, identified by HPLC-DAD-ESI-MS. Cell free antioxidant capacity of unfermented apple juice extracts (AEs) was decreased after fermentation by 30-50%. In the human colon carcinoma cell line Caco-2, F-AEs (containing <0.5% of original AE-phenolics) decreased the reactive oxygen species (ROS) level more efficiently than the F-blank (fermented without AE) but were less effective than the respective AEs. Similarly, antioxidant effectiveness of individual degradation products was lower compared to respective AE constituents. Glutathione level was slightly increased and oxidative DNA damage slightly decreased by fermented AE03, rich in quercetin glycosides. In conclusion, F-AEs/degradation products exhibit antioxidant activity in colon cells but to a lesser extent than the respective unfermented AEs/constituents.  相似文献   

Zu Prekler’s „rationellem Baldwirth“     

M. v. Cotta  M. v. Cotta  Mördes  v. Pannewib  Robert Rühn  Paur  v. Stelzl  v. Deltheim  Schulze  Q. v. Mikleben  Lreiherr v. Lflanteuffel  Lreiherr v. Berg  Qeitel  M. Lreiherr v. Berg  v. Michael  H. C. Bär  Baer  A. Chieriod  Berger  Rettftadt  Gekner  Wlrici  Lreiherr v. Schmerzing  Sprenger  Pommrich  v. Strune  Matufchka v. E.  Q. Brunft  Rnaudt  Mördlinger  Rnipping  v. Mangenheim  C. A. Baum  L. Mehlborn  G. v. Stieglik  H. v. Biegefar  C. Grebe  v.n Baumbach  Braun  R. Blohmer 《European Journal of Forest Research》1865,9(1):322-324

Ohne Zusammenfassung f. f?chf. Dberforftmeifter. f. bahr. Regierungs- und Forftrath von Mürzburg. f. preuk. Dberforftmeifter aus Breslau. f. f?chf. Dberforftmeifter. f. bahr. Regierungs- und Forftrath von Augsburg. f. bahr. Regierungs- und Forftrath aus Regensburg. Dberj?germeifter von Braunfchweig. f. bahr. Regierungs- und Forftrath aus München. f. f?chf. Dberf?rfter. f. f?chf. Dberforftmeifter. f. f?chf. Dberforftrath. herz. braunfchweig. Forftmeifter von Blanfenburg. Direftor der fürftlich Cfterhazh’fchen Herrfchaft Spoly Parsto. fürftl. fondershauf. Dberfortmeifter. f. bahr. Redierf?rfter don Schmarzenbach. f. preuk. Dberf?rfter. f. f. Forftrath aus Brekburg. f. preuk. Dberf?rfter von Altruppin. f. hann?v. Forftmeifter von Rienburg. Forftmeifter in preuk. Schleften. f. preuk. Dberforftmeifter von Berlin. herz. aftenburg. Dberfortmeifter. f. preuk. Dberf?rfter. f. f?chf. Dberf?rfter. f. ruff. Forftfomiff?r aus Marfchau. f. preuk. Forftinfpeftor. f. f?chf. Forftinfpeftor. grokh. meimar. Forftinfpeftor von Martfuhl. f. mürttb. Dberf?rfter und Brofeffor aus Hohenheim. f. hannov. Forftmeifter aus Hammeln. Dberforftmeifter aus Sotha. herz. altenburg. Dberf?rfter aus Hummelshain. herz. altenburg. Revierf?rfter aus Milchwig. herz. altenburg. geheimer Finanzrath und Dberforftmeifter. herz. altenb. Dberforftmeifter aus Hummelshain. grokh. f?chf. geheimer Dberforftrath aus Qifenach. herz. naffau. Dberforftrath aus Miesbaden. grokh. heff. Dberforftfefret?r aus Darmftadt. f. f?chf. Dberforftmeifter.  相似文献   

Bronchopneumonia and polyarthritis due to Mycoplasma bovis in a calf     

Hewicker-Trautwein M  Peters M  Gruber A  Baum B  Liverköhne I  Buchenau I  Kleinschmidt S 《DTW. Deutsche tier?rztliche Wochenschrift》2003,110(4):147-150

This case report describes gross lesions and histopathological findings in a 3-months-old calf originating from a feedlot with approximately 400 cattle. In this animal and additional 14 cattle of similar age, which were kept together in the same stable, swollen joints had occurred suddenly. The examination of this calf showed that a severe polyarthritis induced by haematogenous spread of Mycoplasma bovis following bronchopenumonia was present, which was characterised by necrotising lesions of the joint capsules and severe cartilage erosions.  相似文献   

Analysis of Genetic Diversity in Barley Cultivars Reveals Incongruence Between S-SAP,SNP and Pedigree Data     

V. D. Soleimani  B. R. Baum  D. A. Johnson 《Genetic Resources and Crop Evolution》2007,54(1):83-97

Accurate assessment of genetic similarity is important for plant breeding, germplasm enhancement and conservation of plant genetic resources. A comparative analysis of genome diversity among a group of six-rowed spring barley (Hordeum vulgare L.) cultivars was carried out using sequence-specific amplified polymorphism (S-SAP) and single nucleotide polymorphism (SNP), with the results compared to the kinship coefficients derived from the pedigree data. Mean pair-wise GS values were estimated to be 0.0957 ± 0.144 (Kinship), 0.491 ± 0.189 (SNPs), and 0.602 ± 0.098 (S-SAPs). S-SAP and SNP-based genetic similarity (GS) values were normally distributed but kinship values had a non-normal and skewed distribution. Pair-wise correlation of GS values were lowest for the S-SAP and the SNP matrices (r =; 0.040, p<0.230) and highest for the SNP and pedigree matrices (r =; 0.240, p < 0.001). Analysis of molecular variance (AMOVA) attributed about 90.4% of observed variation to the cultivars within each of the malting and feed groups. Variance component between malting and feed groups was 6.6% for both SNP and S-SAP data suggesting lack of a significant genetic differentiation along this agronomic division. The remaining 3% of variation was attributed to genetic diversity within cultivars. Although both DNA-based marker systems were able to differentiate all barley cultivars, significant difference were observed in the pattern of genetic relationships obtained by the two marker systems and the pedigree data.  相似文献   

Agreement of high definition oscillometry with direct arterial blood pressure measurement at different blood pressure ranges in horses under general anaesthesia          下载免费PDF全文

Karsten Feige  Sabine BR Kästner 《Veterinary anaesthesia and analgesia》2015,42(3):286-291

ObjectiveTo determine the agreement of high definition oscillometry (HDO) with direct arterial blood pressure measurements in normotensive, hypotensive and hypertensive horses during general anaesthesia.Study designExperimental study.AnimalsSeven healthy warmblood horses, aged 3–11 years, weighing 470–565 kg.MethodsMeasurements from a HDO device with the cuff placed around the base of the tail were compared with pressures measured invasively from the facial artery. High blood pressures were induced by intravenous (IV) administration of dobutamine (5 μg kg⁻¹ minute⁻¹) over ten minutes followed by norepinephrine (0.1 mg kg⁻¹ IV) and low pressures by increasing the inspired fraction of isoflurane and administration of nitroglycerine (0.05 mg kg⁻¹ IV). For analysis three pressure levels were determined: high (MAP>110 mmHg), normal (60 mmHgResultsA total of 245 paired measurements of systolic (SAP), mean (MAP) and diastolic (DAP) pressures were obtained. The HDO device underestimated blood pressure at hypertensive and normotensive levels and overestimated blood pressure at hypotensive levels. Best agreement was obtained for SAP and MAP within normotensive limits. At normotension, bias ± standard deviation for SAP, MAP and DAP were 0.1 ± 19.4 mmHg, 0.5 ± 14.0, 4.7 ± 15.6, respectively. At high pressure levels bias and SD were 26.1 ± 37.3 (SAP), 4.2 ± 19.4 (MAP), 1.5 ± 16.8 (DAP) and at low pressures -20.0 ± 20.9 (SAP), -11.4 ± 19.6 (MAP), -4.7 ± 20.1 (DAP), with HDO measurements at a MAP <50 mmHg often failing.Conclusion and clinical relevanceGood agreement with invasive arterial blood pressures was obtained with HDO at normotensive levels in horses. At high and low pressure ranges HDO was unreliable. Therefore, if haemodynamic instability is expected, invasive measurement remains preferable.  相似文献   

The effect of footrot on body weight and wool growth of sheep   总被引：1，自引：0，他引：1  

DJ MARSHALL  RI WALKER  BR CULLIS  MF LUFF 《Australian veterinary journal》1991,68(2):45-49

Body weight and traits associated with production of wool were measured over a 2-year period between 1985 and 1987 in south-western New South Wales in a flock of Merino wethers experimentally infected with footrot. The disease was allowed to spread freely amongst 150 of the flock but kept at very low prevalence in the remaining 50 by preventive footbathing during transmission periods. Severe, underrunning footrot had a significant adverse effect on body weight, for each year of the trial. Body weight was most severely reduced at times of the year when footrot was spreading among animals and lesions were severe. The mean body weight of the infected group at the end of the 2 years of observation was 7.3 kg (11.6%) below that of the control group. Footrot also depressed wool growth, with the mean clean fleece weight of the infected group being 0.4 kg (8%) lighter than that of the controls at each of the 2 annual shearings. There were no consistent differences between the groups for the other wool characteristics measured.  相似文献   

New molecular markers linked to qualitative and quantitative powdery mildew and scald resistance genes in barley for dry areas     

Haitham Sayed  Gunter Backes  Hamed Kayyal  Amor Yahyaoui  Salvatore Ceccarelli  Stefania Grando  Ahmad Jahoor  Michael Baum 《Euphytica》2004,135(2):225-228

A partial genetic linkage map was constructed on 71 doubled-haploid lines derived from a cross between the barley lines Tadmor and WI2291 with 181 molecular markers. The segregating population was used to detect markers linked to the gene Mlg conferring resistance to powdery mildew (Erysiphe graminis f. sp. hordei) and to genes for quantitative resistance to scald (Rhynchosporium secalis). The gene Mlg on chromosome 4H was flanked by two AFLP markers at a distance of 2.0 and 2.4 cM, respectively. QTLs for resistance to scald were detected on chromosomes 2H and 3H. This association of molecular markers with qualitative and quantitative disease resistance loci represents a valuable starting-point for marker-assisted selection. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Segregation distortion in doubled haploid lines of barley (Hordeum vulgare L.) detected by simple sequence repeat (SSR) markers     

Haitham Sayed  Hamed Kayyal  Luke Ramsey  Salvatore Ceccarelli  Michael Baum 《Euphytica》2002,125(2):265-272

A total of 147 simple sequence repeat (SSR) markers (including86 barley and 61 wheat microsatellite markers) were tested for their segregation in a doubled haploid (DH) and an F₂ population of barley. The DH population consisted of 71 doubled haploid lines, developed from F₁ plants of a cross between Tadmor and WI2291using isolated microspore culture technique. A genetic linkage map consisting of 43 microsatellite markers was constructed using the DH population. Particularly on chromosome 4H microsatellite markers showed distorted segregation ratios. Segregation of DH lines based on molecular markers were compared with segregation of 92 F₂ lines from the same cross. The proportion of loci deviating from the expected monogenic segregation ratios in the DH population was significantly higher (19/43loci, 44%) than in the F₂ population (7/43 loci, 16%). The deviation was biased towards the WI2291 parent alleles. In line with this observation, WI2291 was found to perform better than Tadmor in regenerating green plantlets with the isolated microspore-culture technique. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献