全文获取类型
收费全文 | 278篇 |
免费 | 49篇 |
专业分类
林业 | 3篇 |
农学 | 9篇 |
基础科学 | 1篇 |
39篇 | |
综合类 | 16篇 |
农作物 | 5篇 |
水产渔业 | 52篇 |
畜牧兽医 | 188篇 |
园艺 | 6篇 |
植物保护 | 8篇 |
出版年
2023年 | 2篇 |
2021年 | 8篇 |
2020年 | 9篇 |
2019年 | 9篇 |
2018年 | 19篇 |
2017年 | 26篇 |
2016年 | 16篇 |
2015年 | 15篇 |
2014年 | 25篇 |
2013年 | 28篇 |
2012年 | 15篇 |
2011年 | 23篇 |
2010年 | 18篇 |
2009年 | 20篇 |
2008年 | 12篇 |
2007年 | 10篇 |
2006年 | 12篇 |
2005年 | 10篇 |
2004年 | 6篇 |
2003年 | 7篇 |
2002年 | 4篇 |
2001年 | 2篇 |
1999年 | 5篇 |
1998年 | 4篇 |
1997年 | 8篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1992年 | 1篇 |
1990年 | 3篇 |
1988年 | 1篇 |
1986年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
排序方式: 共有327条查询结果,搜索用时 62 毫秒
1.
Juan Pablo Zoffoli Bernardo A. Latorre Paulina Naranjo 《Postharvest Biology and Technology》2009,51(2):183-192
Over 54,600 ha of table grapes (Vitis vinifera), mainly cvs. ‘Thompson Seedless’, ‘Flame Seedless’ and ‘Redglobe’, are planted in Chile. Almost the entire production is exported to the USA, Europe, Asia, or one of several Latin American countries, which typically requires 15–40 d of maritime transportation. During this period, several physical, physiological, and pathological factors cause table grape deterioration. Because berry size is the main quality factor in international markets, farmers often overuse the growth regulators, gibberellic acid (GA3) and forchlorfenuron (CPPU), in an effort to increase berry size. We examined the effect of preharvest growth regulators on seedless (‘Thompson Seedless’, and ‘Ruby Seedless’) and seeded (‘Redglobe’) table grape cultivars during cold (0 °C) storage plus a shelf life period of 3 d at 20 °C. The overuse of GA3, eight instead of two GA3 applications on Thompson Seedless, and the use of one GA3 application on Redglobe and ‘Ruby Seedless’, increased berry pedicel thickness and lowered cuticle content but induced shatter and predisposed grapes to gray mold caused by Botrytis cinerea. In contrast, CPPU increased berry pedicel thickness and cuticle content but did not increase shatter or gray mold incidence. Clusters that were subjected to overuse of combined GA3 and CPPU were highly sensitive to shatter, had the thickest pedicel, and developed a high gray mold incidence during cold storage. Hairline, a fine cracking developed during cold storage, was induced on ‘Thompson Seedless’ and ‘Ruby Seedless’ by growth regulators, but no hairline occurred on ‘Redglobe’ table grapes. Therefore, berry quality during cold storage is greatly influenced by growth regulator management in the vineyard. 相似文献
2.
AE Jackson 《Australian veterinary journal》2015,93(12):431-432
3.
Mohammadreza Ebrahimi Laura Mara Bernardo Chessa Fabrizio Chessa Abbas Parham Maria Dattena 《Reproduction in domestic animals》2021,56(3):467-475
Microinjection of exogenous DNA into the cytoplasm of matured oocytes or zygotes is a promising technique to generate transgenic animals. However, the data about the microinjection time and procedure in sheep are limited and have not treated in detail. To obtain more in-depth information, the Sarda sheep oocytes from abattoir-derived ovaries were subjected to IVM and IVF. Then, the GFP plasmid as a reporter gene was injected into the cytoplasm of MII oocytes (n: 95) and zygotes at different post-insemination intervals (6–8 hpi, n: 120; 8–10 hpi, n: 122; 10–12 hpi, n: 110 and 12–14 hpi, n: 96). There were no significant differences in the cleavage rates between the groups. However, blastocyst rate of injected zygotes at all-time intervals was significantly lower than injected MII oocytes and control group (p < 0.05). Interestingly, the proportion of GFP-positive embryos was higher at 8–10 hpi compared with other injected groups (4 % versus 0 %, p < 0.01). Among these, the proportion of mosaic embryos was high and two of those embryos developed to the blastocyst stage. In conclusion, we settled on the cytoplasmic microinjection of GFP plasmid at 8–10 hpi as an optimized time point for the production of transgenic sheep and subsequent experiments. 相似文献
4.
Alisha Wehdnesday Bernardo Reyes Hannah Leah Tadeja Simborio Huynh Tan Hop Lauren Togonon Arayan Suk Kim 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(1):119-122
The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis. 相似文献
5.
AE Jackson 《Australian veterinary journal》2016,94(11):395-396
6.
AE Jackson 《Australian veterinary journal》2011,89(6):197-198
Computer model predictions and field observations of anthelmintic resistance in sheep · Dangers of off‐label use of barium selenate · Elbow luxation in dogs and cats · Prognosis of joint infections in adult horses · Omentalisation for mediastinal abscess in a dog · Adenoviruses in lizards 相似文献
7.
8.
9.
10.