全文获取类型
| 收费全文 | 97篇 |
| 免费 | 3篇 |
专业分类
| 林业 | 5篇 |
| 农学 | 7篇 |
| 基础科学 | 2篇 |
| 35篇 | |
| 综合类 | 5篇 |
| 农作物 | 15篇 |
| 水产渔业 | 9篇 |
| 畜牧兽医 | 15篇 |
| 园艺 | 3篇 |
| 植物保护 | 4篇 |
出版年
| 2022年 | 2篇 |
| 2021年 | 3篇 |
| 2020年 | 2篇 |
| 2019年 | 3篇 |
| 2018年 | 5篇 |
| 2017年 | 4篇 |
| 2016年 | 5篇 |
| 2015年 | 1篇 |
| 2014年 | 3篇 |
| 2013年 | 10篇 |
| 2012年 | 7篇 |
| 2011年 | 3篇 |
| 2010年 | 2篇 |
| 2009年 | 2篇 |
| 2008年 | 4篇 |
| 2007年 | 2篇 |
| 2006年 | 5篇 |
| 2005年 | 1篇 |
| 2004年 | 4篇 |
| 2003年 | 9篇 |
| 2002年 | 3篇 |
| 2001年 | 1篇 |
| 2000年 | 1篇 |
| 1997年 | 2篇 |
| 1996年 | 1篇 |
| 1993年 | 1篇 |
| 1988年 | 2篇 |
| 1987年 | 2篇 |
| 1985年 | 3篇 |
| 1984年 | 1篇 |
| 1981年 | 1篇 |
| 1980年 | 1篇 |
| 1979年 | 1篇 |
| 1971年 | 1篇 |
| 1965年 | 2篇 |
排序方式： 共有100条查询结果，搜索用时 296 毫秒
1.
2.
Polymerase chain reaction (PCR) was used to amplify the spacer regions between the 16S and 23S genes of rRNA genetic loci of Salmonella serovars for their rapid identification. These genetic loci revealed a significant level of polymorphism in length across the species/serovar lines. When the 16S-23S spacer region amplification products were subjected to agarose electrophoresis, the patterns observed could be used to distinguish all the serovars of Salmonella tested. Unique elements obtained in amplification products were mostly clustered at serovar level, although certain genus-specific patterns were also observed. On the basis of the results obtained, the amplification of 16S-23S ribosomal spacer region could suitably be used in a PCR-based identification method for Salmonella serovars. 相似文献
3.
I. Tetens S.K. Biswas L.V. Glitsø K.A. Kabir S.H. Thilsted N.H. Choudhury 《Journal of Cereal Science》1997,26(3):355-361
The hypothesis was tested that certain physico-chemical characteristics might be used as indicators of total starch availability and rate of starch availability of milled rice. Milled unparboiled (uPB) and parboiled (PB) rice samples (n=93) were characterized using standardized methods of physical tests and chemical analyses and anin vitromethod was used for measuring the rate of starch digestion on a subsample of rice (n=26). The rice varieties were dominated by medium long, bold rice grain with high amylose rice and intermediate gelatinization temperature (GT), but a wide range in all characteristics was measured. Small amounts of resistant starch (RS) were measured in the cooked rice, indicating virtually complete starch availability. The RS of PB rice (0·4 g/100 g rice as eaten) was significantly (P<0·004) higher than the RS of uPB rice (0·1 g/100 g) however. The rate of starch digestion was significantly affected by both variety and parboiling. The starch digestion index (SDI) values of the PB samples (mean value 73·7) were significantly (P<0·001) lower than those of the uPB samples (mean value 79·0). The apparent amylose content (AC) was the strongest determinant for SDI in both uPB and PB rice. The widths and shapes of the raw grains and the elongation after cooking were correlated significantly with SDI values for the uPB rice, while the relatively mild parboiling procedure followed in this study eliminated this correlation. The minimum cooking times were correlated significantly with the SDI values in the uPB samples. 相似文献
4.
Chanda D Debnath SC Das SK Mandal TK Bhattacharyya A Choudhury A Chakraborty AK 《Journal of agricultural and food chemistry》2004,52(24):7377-7381
Disposition kinetic behavior and metabolism studies of metamitron and its metabolite in terms of the parent compound were carried out in black Bengal goats after a single oral administration of a nontoxic oral dose at 30 mg kg(-1) of body weight. Metamitron was detected in the blood sample at 5 min (2.23 +/- 0.04 microg mL(-1)), maximum at 1 h (3.43 +/- 0.02 microg mL(-1)) and minimum at 12 h (0.41 +/- 0.01 microg mL(-1)), after a single oral administration. Metabolite [3-methyl-6-phenyl-1,2,4-triazin-5(4H)-one] in terms of the parent compound was detected in the blood sample at 5 min (0.47 +/- 0.006 microg mL(-1)), maximum at 6 h (5.12 +/- 0.02 microg mL(-1)) and minimum at 96 h (1.06 +/- 0.016 microg mL(-1)), after a single oral administration. The t(1/2 K) and Cl(B) values of metamitron were 3.63 +/- 0.05 h and 1.36 +/- 0.016 L kg(-1) h(-1), respectively, whereas the t(1/2K)(m) and Cl(B)(m) values of the metabolite were 38.15 +/- 0.37 h and 0.091 +/- 0.001 L kg(-1) h(-1), respectively, which suggested long persistence of the metabolite in blood and tissues of goat. Metamitron was excreted through feces and urine for up to 48 and 72 h, whereas the metabolite was excreted for up to 168 and 144 h, respectively. Metabolite alone contributed to 96 and 67% of combined recovery percentage of metamitron and metabolite against the administered dose in feces and urine of goat, respectively. All of the goat tissues except lung, adrenal gland, ovary, testis, and mammary gland retained the metabolite residue for up to 6 days after administration. 相似文献
5.
Bhaskar Choudhury 《Agricultural and Forest Meteorology》1985,34(4):333-336
Well-watered crops having nearly identical ratio of intercept and slope of the “non-water-stressed baseline” would attain their equivalence point temperature at roughly identical air vapor pressure deficit. However, such a behavior of these crops might not prevail as the wet soil dries out progressively. The methods for calculating the equivalence point temperature due to Linacre (1964) and Paw U (1984) gave results which sometimes differed by more than 10 K. 相似文献
6.
Bhaskar Sharma B. S. Negi A. B. Pandey S. K. Bandyopadhyay H. Shankar M. P. Yadav 《Tropical animal health and production》1988,20(2):109-113
Summary The counterimmunoelectrophoresis (CIE) test was standardised for the detection of goat pox antigen and antibody using inactivated antigens. The chloroform inactivated and live antigens were equally sensitive for detection of goat pox precipitins. The precipitinogens of goat pox virus (GPV) were found to be soluble in nature. The CIE test was quick as well as more sensitive than the agar gel precipitation test for detection of GPV antibody/antigen. The CIE employing inactivated antigen has been used for the first time in the detection of GPV antibodies/antigens.
Deteccion Del Antigeno Y Anticuerpos De Viruela Caprina Mediate La Prueba De Contrainmunoelectroforesis
Resumen Se estandarizó la prueba de contrainmunoelectroforesis, para la detección del antígeno y anticuerpos del virus de la viruela caprina, usando antígenos inactivados. El antígeno inactivado con cloroformo y el antígeno vivo, fueron igualmente sensitivos para la detección de precipitinas de viruela caprina. Los precipitógenos del virus de la viruela caprina se encontraron que eran solubles. La prueba de contrainmunoelectroforesis fue más rápida y más sensitiva que la precipitación agar gelatina para la detección de anticuerpos/antígenos del virus de la viruela caprina. La prueba de contrainmunoelectroforesis con antígeno inactivado ha sido utilizada por vez primera en la detección de anticuerpos/antígenos del virus de la viruela caprina.
Detection De l'Antigene Et De l'Anticorps Variole Caprine Par Un Test De Contrimmuno-Electrophorese
Résumé Le test de contrimmuno-électrophorèse (CIE) a été standardisé pour la détection de l'antigène et de l'anticorps variole caprine avec des antigènes inactivés. Les antigènes vivants et inactivés par le chloroforme sont de sensibilité équivalente pour la détection des précipitines variole caprine. On a montré que ces précipitogènes du virus variole caprine (VVC) étaient de nature soluble. Le CIE est rapide et plus sensible que le test de précipitation en gélose pour la détection des antigènes et anticorps VVC. C'est la première fois que le CIE mettant en oeuvre un antigène inactivé a été utilisé.相似文献
7.
V. S. A. K. Sama K. Himabindu S. Bhaskar Naik R. M. Sundaram B. C. Viraktamath J. S. Bentur 《Euphytica》2012,187(3):393-400
Host plant resistance is the preferred management strategy for Asian rice gall midge (Orseolia oryzae), a serious pest in many rice-growing countries. Identification of simple sequence repeat (SSR) markers that are tightly linked to pest resistance genes can accelerate development of gene pyramids for durable/multiple resistance. Based on conventional and molecular allelism tests, we report herein that rice genotype Aganni possesses Gm8 gene, conferring hypersensitive independent (HR– type) resistance to gall midge biotypes GMB1, GMB2, GMB3, GMB4, and GMB4M. The gene Gm8 was mapped to chromosome 8 within a 400-kbp region, and the SSR markers RM22685 and RM22709 flank the gene closely. Using these closely linked flanking markers, nine other gall midge-resistant genotypes were identified as carrying the same gene Gm8. Through marker-assisted selection, Gm8 has been introgressed into an elite bacterial blight-resistant cultivar, Improved Samba-Mahsuri (IS). 相似文献
8.
Shanti Shrestha Bhaskar Singh Karky Anup Gurung Rajesh Bista Ole Reidar Vetaas 《Small-Scale Forestry》2013,12(4):507-517
This study assessed the net above-ground carbon stock in six community forests in the Dolakha district, Nepal. A survey was conducted of above-ground timber species, using random sampling. A tree-ring chronology for Pinus roxburghii was created to construct a growth model representative of the various mainly-pine species. The allometric model combined with tree ring analysis was used to estimate carbon stock and annual growth in the above-ground tree biomass. The out-take of forest biomass for construction material and fuelwood was estimated on the basis of interviews and official records of community forest user groups. The average annual carbon increment of the community forests was 2.19 ton/ha, and the average annual carbon out-take of timber and fuelwood was 0.25 ton/ha. The net average carbon balance of 1.94 ton/ha was equivalent to 117.44 tons of carbon per community forest annually. All the community forests were actively managed leading to a sustainable forest institution, which acts as a carbon sink. It is concluded that community forests have the potential to reduce emissions by avoiding deforestation and forest degradation, enhance forest carbon sink and improve livelihoods for local communities. 相似文献
9.
10.
Sujata Mohapatra Rajesh Kumar Srianga Tilak Patnaik Choudhury Suryakant Mishra Lakshman Sahoo Jitendra Kumar Sundaray 《Aquaculture Research》2020,51(8):3230-3236
Monocrotophos (MCP) is one of the organophosphate pesticides extensively used in agriculture and animal husbandry. The present study reports the effects of sub‐lethal concentrations of MCP on gonadosomatic index (GSI), gonadal histology and breeding fitness of the fish Anabas testudineus. The fish were exposed to three sub‐lethal concentrations of monocrotophos (T1: 3.5 mg/L, T2: 5.3 mg/L and T3: 10.6 mg/L) for 45 days with 12 fish (males and females in equal numbers) in each tank, and one group was kept as control (C) without any treatment. GSI decreased in all treatment groups in comparison with control. Significant decrease in fertilization and hatching rate was observed at all concentrations in comparison with control on 15, 30 and 45 days of monocrotophos exposure. The decreased vitellogenesis, disruption of follicular wall and oocyte atresia were observed in all the treated females at 30 and 45 days of exposure. Rupture of seminiferous tubules was noticed in all the treated males irrespective of exposure periods. The results thus indicated that monocrotophos could deleteriously impact the gonadal structure and function in A. testudineus, which could seriously impact the reproductive success of the animal. 相似文献