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月季花瓣衰老过程中可溶性蛋白的SDS—PAGE分析 总被引:8,自引:0,他引:8
对月季花瓣衰老过程中可溶性蛋白的SDS-PAGE进行了分析,结果表明,蛋白条带变化表现出3种情况:(1)从瓶插开始到结束蛋白条带基本保持稳定;(2)随花瓣衰老,蛋白条带逐渐减弱、消失;(3)随着花的衰老,有新的蛋白条带出现。对花进行ABA或乙烯促衰处理,也导致相应的某些蛋白条带的变化,说明这些蛋白的变化可能与衰老有关。 相似文献
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不同温度差压预冷及其对西葫芦冷藏效果的影响 总被引:2,自引:2,他引:0
为了延长西葫芦的采后保鲜期和减少营养物质的损耗,本文以西葫芦(Cucurbita pepo.L)为试材,比较了不同预冷温度对西葫芦预冷后的冷藏效果,分别置于-3、0、3和6℃差压冷库中,预冷到10℃,然后在10℃条件下冷藏。期间每4 d取一次样,测定其各项指标。结果表明:6℃预冷速度最慢,0和3℃快于6℃,-3℃预冷速度最快。与冷藏前相比,冷藏结束(16 d)时,0和3℃预冷组的丙二醛含量分别上升了66.76、71.63%,显著(P0.05)低于其他2个处理;4个组(分别为-3,0,3和6℃预冷组)的硬度分别下降了9.89%、7.46%、7.89%和11.19%;CAT活性分别为16.25、19.79、17.58和16.63 U/min?g;各组的维生素C含量分别下降了32.61%、20.65%、26.09%和30.87%;香气成分中醛类相对含量分别为69.94%、76.33%、71.47%和70.97%。与-3和6℃预冷相比,0和3℃预冷处理能够显著的延缓硬度的下降,降低呼吸速率和乙烯的释放量,因此能延缓西葫芦的采后衰老和延长货架期;并抑制了丙二醛的积累,减少组织损伤且抗坏血酸过氧化物酶活性和过氧化物酶的活性也受到抑制,提高了过氧化氢酶活性,因而可以降低活性氧伤害;并保持了较高的香气成分含量,而-3和6℃预冷的西葫在冷藏后期的呼吸强度及抗坏血酸过氧化物酶活性迅速升高,且-3℃的预冷处理使得西葫芦在之后的冷藏过程中出现了明显的冷害现象,即表皮长斑。因此0和3℃差压预冷对西葫芦的冷藏效果较好,研究结果为西葫芦采后冷链技术的发展提供了参考。 相似文献
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Yumei M. Jiang Chengrong R. Chen Yuanqiu Q. Liu Zhihong H. Xu 《Journal of Soils and Sediments》2010,10(6):1071-1081
Purpose
The objective of the present study was to assess the differences in soil total C and N, microbial biomass C and N, soil soluble organic C and N among eight mono- and mixed species forest ecosystems (18-year-old restoration) in subtropical China. 相似文献7.
Soil microbial biomass, activity and community composition in adjacent native and plantation forests of subtropical Australia 总被引:2,自引:0,他引:2
Joanne Burton Chengrong Chen Zhihong Xu Hossein Ghadiri 《Journal of Soils and Sediments》2010,10(7):1267-1277
Purpose
Soil nitrogen (N) availability is a critical determinant of plantation productivity in subtropical Australia and is influenced by the soil microbial community. The size, structure and function of the soil microbial community can be impacted by land-use change and residue management. The objectives of this study were to examine the impact of land-use change from (1) native forest (NF) to first rotation (1R) hoop pine plantation and (2) 1R hoop pine plantation to second rotation (2R) hoop pine plantation on the soil microbial community. The impact of residue management on the soil microbial community was also investigated in the 2R forest, where soil microbial parameters were measured in tree rows (2R-T) and windrows (2R-W). In addition, relationships between soil microbial parameters and soil N parameters were investigated.Materials and methods
Each of the four treatments (NF, 1R, 2R-T and 2R-W) had five 24-m2 replicate plots from which 15 soil cores were collected and bulked at three depths (0–10, 10–20, 20–30 cm). Microbial biomass carbon (MBC) and N (MBN) and soil respiration were measured on field moist soils. In addition, carbon (C) source utilisation patterns were assessed using the whole soil MicroResp? technique (Campbell et al. 2003).Results and discussion
Results indicate that the land-use change from NF to 1R hoop pine plantation significantly reduced MBC, respiration rate, soil total C and total N. Furthermore, the land-use change appeared to have a significant impact on the soil microbial community composition measured using MicroResp? profiles. Land-use change from 1R to 2R hoop pine plantation resulted in a decline in total C and MBN and a shift in microbial community composition. When compared to the 2R-T soils, the 2R-W soils tended to have a greater microbial biomass and respiration rate. Residue management also influenced the microbial community composition measured in the MicroResp? profiles.Conclusions
Results indicate that land-use change had a significant impact on the soil microbial community, which was likely to be related to shifts in the quality and quantity of organic inputs associated with the change in land use. This may have significant implications for the long-term productivity of the soil resource. Further studies are required to confirm a difference in microbial community composition associated with residue management. In addition, long-term experiments in subtropical Australia are necessary to verify the results of this snapshot study and to improve our understanding of the impact of single-species plantation forestry and residue management on the soil microbial community, soil N dynamics and ultimately the long-term sustainability of the soil resource. 相似文献8.
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为提高毛竹林的生物生产力,在广西资源县中峰乡选择不同经营管理措施毛竹人工林设置土壤剖面和取样,按国家颁布标准测定其土壤理化性质。结果表明:(1)0~40 cm土层的土壤密度为0.735~1.14 g.cm-3;土壤总孔隙度为44.85%~58.56%;土壤通气度为30.7%~55.4%;土壤最大持水量、毛管持水量和最小持水量依次为53.87%~92.02%、37.05%~62.49%和21.21%~34.23%。(2)0~40 cm土层的土壤pH值为4.62~4.90;土壤有机质含量为16.78~79.37 g.kg-1,土壤全N、全P、全K含量依次为1.18~4.01 g.kg-1、0.94~1.91 g.kg-1和22.48~42.26 g.kg-1。(3)全垦抚育方式的土壤密度比不抚育方式略小;土壤孔隙度及持水量比不抚育方式略大;土壤有机质及N、P、K养分含量比不抚育方式略高。研究结果充分说明,该区毛竹林地土壤疏松、通气、透气性能良好,土壤酸减度适中,土壤有机质以及N、P、K养分含量较高,这些研究结果为该区毛竹人工林的经营管理与评价提供了重要的参考价值。 相似文献
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Responses of soil dissolved organic matter to long-term plantations of three coniferous tree species
Tree species have significant effects on the availability and dynamics of soil organic matter. In the present study, the pool sizes of soil dissolved organic matter (DOM), potential mineralizable N (PMN) and bio-available carbon (C) (measured as cumulative CO2 evolution over 63 days) were compared in soils under three coniferous species — 73 year old slash (Pinus elliottii), hoop (Araucaria cunninghamii) and kauri (Agathis robusta) pines. Results have shown that dissolved organic N (DON) in hot water extracts was 1.5–1.7 times lower in soils under slash pine than under hoop and kauri pines, while soil dissolved organic C (DOC) in hot water extracts tended to be higher under slash pine than hoop and kauri pines but this was not statistically significant. This has led to the higher DOC:DON ratio in soils under slash pine (32) than under hoop and kauri pines (17). Soil DOC and DON in 2 M KCl extracts were not significantly different among the three tree species. The DOC:DON ratio (hot water extracts) was positively and significantly correlated with soil C:N (R2 = 0.886, P < 0.01) and surface litter C:N ratios (R2 = 0.768, P < 0.01), indicating that DOM was mainly derived from litter materials and soil organic matter through dissolution and decomposition. Soil pH was lower under slash pine (4.5) than under hoop (6.0) and kauri (6.2) pines, and negatively correlated with soil total C, C:N ratio, DOC and DOC:DON ratio (hot water extracts), indicating the soil acidity under slash pine favored the accumulation of soil C. Moreover, the amounts of dissolved inorganic N, PMN and bio-available C were also significantly lower in soils under slash pine than under hoop and kauri pines. It is concluded that changes in the quantity and quality of surface litters and soil pH induced by different tree species largely determined the size and quality of soil DOM, and plantations of hoop and kauri pine trees may be better in maintaining long-term soil N fertility than slash pine plantations. 相似文献