绿矾对防治苹果黄叶病效应的初步研究     

周正卿 《植物保护学报》1964,3(2):145-154

以适当浓度的綠矾溶液;或以綠矾——檸檬酸混合溶液浸泡病株树根,效果显著,病株复綠快速,惟不能持久。若以綠矾——有机混合肥料施于根区,防治效果良好,效力持久,但复綠較慢。综合利用这两种施用方法,卽:先用綠矾溶液浸泡树根;后用綠矾——有机混合肥料施于根区,則可达到防病快速持久的目的。  相似文献   

Determination of plasma lysosomal enzyme activity and its relationship to severity of injury following blunt vehicular trauma in dogs     

Rosalind S. Chow  VMD    Rebecca S. Syring  DVM  DAVECC    Cynthia M. Otto  DVM  PhD  DAVECC 《Journal of Veterinary Emergency and Critical Care》2004,14(2):100-107

Objective: To determine plasma β‐d ‐glucuronidase (βG) activity in the first 4 hours following injury in dogs struck by a motor vehicle, and to evaluate whether the degree of enzyme activity is correlated with the severity of injury. Design: A prospective clinical study. Setting: Veterinary Medical Teaching Hospital. Animals: Thirteen client‐owned dogs that were presented to the Veterinary Hospital of the University of Pennsylvania between June and August 1999 for blunt vehicular trauma. Ten healthy student and staff‐owned dogs served as controls. Interventions: None. Measurements: Plasma was analyzed for βG enzyme activity at the time of presentation (n=13), and 1 and 4 hours (n=7) following presentation to the Emergency Service for blunt vehicular trauma. The results were compared with enzyme activity from healthy controls evaluated serially over 4 hours. Fluorometric analysis using 96‐well microtiter plates was used to perform the enzyme assays. The relationships between presentation (n=13) and 4 hours (n=7) of enzyme activity and 3 indices of metabolic and physical disturbance (serum pH, serum lactate and Animal Trauma Triage (ATT) score) at the time of presentation were also investigated. Main results: Of the 13 dogs, 7 fulfilled the inclusion criteria for comparison of enzyme activity of the trauma over time. A statistically significant difference in βG activity was found in the trauma group (mean 75.6±10.4 U) at 4 hours following presentation compared with controls (mean 48.0±6.4 U). This difference was suggested by 1 hour following presentation (trauma group, mean 70.4±10.9 U; control group, mean 49.8±5.5 U), although it did not reach statistical significance. Thirteen dogs fulfilled the inclusion criteria for comparison of only presentation enzyme activity with trauma severity score, serum lactate, and serum pH. No statistically significant relationship was found between the βd ‐glucuronidase activity and the presenting ATT score, serum lactate concentration, or serum pH at either presentation or 4 hours, although the power of these analyses was low. Conclusions: These results demonstrate that the activity of βG, a lysosomal enzyme, increases significantly in the systemic circulation in dogs 4 hours following blunt trauma. Additional research to include more severely injured dogs, a larger number of dogs, and to follow the course of injury for a longer period of time would be beneficial to further characterize βG activity following blunt trauma.  相似文献   

Use of in vitro assays to identify antibiotics that are cytotoxic to normal equine chondrocytes and synovial cells     

Lynn Pezzanite  Lyndah Chow  Gabriella Piquini  Gregg Griffenhagen  Dominique Ramirez  Steven Dow  Laurie Goodrich 《Equine veterinary journal》2021,53(3):579-589

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Characterization of the intestinal mucosal proteome in cats with inflammatory bowel disease and alimentary small cell lymphoma     

Sina Marsilio  Floris C. Dres  Lawrence Dangott  Betty Chow  Steve Hill  Mark Ackermann  J. Scott Estep  Jonathan A. Lidbury  Jan S. Suchodolski  Jrg M. Steiner 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2021,35(1):179-189

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Seroprevalence of and agroecological risk factors for Mycobacterium avium subspecies paratuberculosis and neospora caninum infection among adult beef cattle in cow-calf herds in Alberta, Canada     

Scott HM  Sorensen O  Wu JT  Chow EY  Manninen K 《The Canadian veterinary journal. La revue veterinaire canadienne》2007,48(4):397-406

A province-wide cross-sectional seroprevalence and agroecological risk factor study of Mycobacterium avium subspecies paratuberculosis (MAP) and Neospora caninum (NC) infection among cattle in 100 cow-calf herds in Alberta was conducted. The seroprevalence of MAP in adult cattle was 1.5% across all herds. Using a widely accepted herd test cutpoint of 2 or more seropositive cows out of 30 animals tested, 7.9% of herds were estimated to be infected (95% confidence interval (CI): 2.3-23.4%). Seroprevalence of MAP differed by agroecological region; specifically, cattle and herds in areas with high soil pH (> 7.0), southern latitudes, and arid climates had a moderately reduced risk of infection (P < 0.10). Seroprevalence of NC infection was 9.7% among adult beef cattle province-wide--these levels also varied by agroecological region--with 91.0% of herds infected overall.  相似文献   

Universal PCR primers for ribosomal protein gene introns of fish     

Seinen Chow  Takashi Yanagimoto 《International Aquatic Research》2016,8(1):29-36

Human ribosomal protein (RP) gene sequences with respect to intron/exon structures and corresponding cDNA or genomic data of fish species were obtained from the GenBank database. Based on conserved exon sequences, 128 primer pairs for 41 genes were designed for exon-primed intron-crossing (EPIC) polymerase chain reaction (PCR). In reference to the draft genome sequences of the Pacific bluefin tuna (Thunnus orientalis), 12 primer pairs expected to amplify introns of the bluefin tuna with lengths of 500–1000 bp were selected and applied to six distantly related fish species belonging to the Orders Clupeiformes, Tetraodontiformes, Pleuronectiformes, Perciformes, Scorpaeniformes, and Anguilliformes. PCR amplification was observed for at least four species in each primer pair, and all fragments were larger than those expected for intronless amplification. Single fragment amplification was observed for at least seven primer pairs per species. Fragment sizes of the bluefin tuna for nine primer pairs corresponded to those expected from the genomic data. Thus, our primer pairs are potentially applicable to a wide variety of fish species and serve as an initial step for isolating single-copy nuclear DNA sequences.  相似文献   

Identification of ommastrephid squid paralarvae collected in northern Hawaiian waters and phylogenetic implications for the family Ommastrephidae using mtDNA analysis     

Toshie Wakabayashi  Nobuaki Suzuki  Mitsuo Sakai  Taro Ichii  Seinen Chow 《Fisheries Science》2006,72(3):494-502

ABSTRACT:   A total of 110 adult individuals from four ommastrephid (family Ommastrephidae) squid species ( Ommastrephes bartramii, Sthenoteuthis oualaniensis, Eucleoteuthis luminosa, and Hyaloteuthis pelagica ) were used to obtain diagnostic DNA markers for species identification. Restriction fragment length polymorphism (RFLP) analysis of a partial segment (855 bp) of the mitochondrial DNA cytochrome oxidase I (COI) gene amplified by polymerase chain reaction (PCR) revealed that the restriction profiles of two endonucleases ( Alu  I and Tsp 509 I) were diagnostic for species identification. The restriction assay partially supplemented with nucleotide sequence analysis successfully assigned 69 damaged and morphologically equivocal ommastrephid paralarvae collected in northern Hawaiian waters, identifying 60 O. bartramii , eight S. oualaniensis , and one E. luminosa . The family Ommastrephidae appears to be monophyletic. Although the phylogenetic relationships among genera were not resolved well due to apparent homoplasy and large genetic divergence between species, COI sequence data without transitions provided support for subfamily level relationships.  相似文献   

Clean water: Factors that influence Its availability,quality and its use: Summary of the International Water Conference     

R. W. Brockseni  W. Chow  E. D. Daugherty  Y. G. Mussalli  J. Wisniewski  A. L. Woodis 《Water, air, and soil pollution》1996,90(1-2):3-7

The International Water Conference held in La Jolla, California during 28–30 November 1995, had an objective to provide an opportunity for international information exchange on new research applications and timely policy issues effecting the availability, use and quality of water for power generation. This Conference included the topical areas, the use of water and the management of chemical quality in process wastewaters; physical and biological transformation due to discharges in open surface waters, watersheds and wetlands; the role of risk and cost-benefit assessments; and discussion of the Clean Water Act containments. This Conference Summary contains an overview of the present state of the knowledge regarding the use of water by the electric utility industry and potential future issues as discussed in papers presented at the Conference and a summary of the most important findings and conclusions.  相似文献   

Duration of immunity in heifers inoculated with infectious bovine rhinotracheitis virus   总被引：4，自引：0，他引：4  

T L Chow 《Journal of the American Veterinary Medical Association》1972,160(1):51-54

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Immunodiffusion of bluetongue virus   总被引：4，自引：0，他引：4  

M M Jochim  T L Chow 《American journal of veterinary research》1969,30(1):33-41

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