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1.
Foster JG Clapham WM Belesky DP Labreveux M Hall MH Sanderson MA 《Journal of agricultural and food chemistry》2006,54(5):1772-1778
The forage potential of chicory (Cichorium intybus L.) has not been realized in southern West Virginia (WV) because ruminants are reluctant to consume the herbage. Chicory contains bitter sesquiterpene lactones that can adversely impact palatability. This study was undertaken to determine whether sesquiterpene lactone concentrations in chicory grown in southern WV differ from those in chicory grown in central Pennsylvania (PA) where chicory is grazed readily. Herbage was collected in 1997 and 1998 from cultivars Grasslands Puna (Puna), INIA le Lacerta (Lacerta), and Forage Feast established at research sites near State College, PA, and Beckley, WV. The total concentration of sesquiterpene lactones in WV-grown cultivars was 0.58% (dry matter basis) in Puna, 0.59% in Lacerta, and 0.79% in Forage Feast in 1997 and ranged from 1.03 (Lacerta) to 1.52% (Forage Feast) in 1998. In PA-grown cultivars, sesquiterpene lactones represented 0.16 (Puna), 0.18 (Lacerta), and 0.27% (Forage Feast) of the forage dry matter in 1997 and ranged from 0.32 (Lacerta) to 0.55% (Forage Feast) in 1998. Concentrations of lactucin, lactucopicrin, and total sesquiterpene lactones in Forage Feast exceeded those in the other cultivars grown at the same site. The lowest concentrations of lactucopicrin and total sesquiterpene lactones observed among WV-grown cultivars were higher (2-fold or more) than the highest concentrations present in cultivars grown the same year in PA. Mineral analyses of soils from the two cultivation sites indicate that P availability may influence sesquiterpene lactone composition of chicory herbage. Results provide a foundation for future studies of environmental effects on sesquiterpene lactone composition and palatability of chicory herbage. 相似文献
2.
Forage Chicory: A Plant Resource for Nutrient-Rich Sites 总被引：1，自引：0，他引：1
Grazing livestock create localized nutrient patches that increase soil ionic strength (IS) and influence plant productivity. The ability of plant root systems to control ion absorption and flux to xylem, and to sequester ions reaching leaf tissue in bound, nontoxic forms are means of minimizing IS. A greenhouse experiment was conducted to determine the growth and mineral acquisition responses of forage chicory (Cichorium intybus L. cv. Grasslands Puna) to increasing (0.9, 4, 8, and 12 dS m?1) IS in the rhizosphere obtained by additions of NaCl/CaCl2 (1 : 1 m ratio). Plants were harvested four times after planting (20, 27, 34 and 41 d) to identify responses as a function of time. Increased accumulation and localization of Na in roots in comparison to shoots suggested that chicory restricted Na transport to shoots, and that insoluble Na in tissues increased with increasing IS. Soluble cations in shoots were about 50 % of total cations, irrespective of rhizosphere IS and Na uptake. Differences in the cation:anion ratio could not be accounted for by organic acid concentrations in chicory, but substantial accumulation of nonstructural carbohydrates as fructans in roots could contribute to charge balance. Our results demonstrate that forage chicory has moderate tolerance to IS, suggesting that it might be a useful species for sites with potential for IS. Chicory growth would probably be sustained under IS conditions, and the resultant vigorous growth and accumulation of mineral nutrients in shoots would contribute to acceptable nutritive value for grazing livestock. Plants capable of growing in and acquiring nutrients from nutrient‐laden patches in the sward would help minimize erosion and nutrient transport, with positive benefits for water and soil quality. 相似文献
3.
Intracellular calcium (Ca2+) is a ubiquitous second messenger. Information is encoded in the magnitude, frequency, and spatial organization of changes in the concentration of cytosolic free Ca2+. Regenerative spiral waves of release of free Ca2+ were observed by confocal microscopy in Xenopus laevis oocytes expressing muscarinic acetylcholine receptor subtypes. This pattern of Ca2+ activity is characteristic of an intracellular milieu that behaves as a regenerative excitable medium. The minimal critical radius for propagation of focal Ca2+ waves (10.4 micrometers) and the effective diffusion constant for the excitation signal (2.3 x 10(-6) square centimeters per second) were estimated from measurements of velocity and curvature of circular wavefronts expanding from foci. By modeling Ca2+ release with cellular automata, the absolute refractory period for Ca2+ stores (4.7 seconds) was determined. Other phenomena expected of an excitable medium, such as wave propagation of undiminished amplitude and annihilation of colliding wavefronts, were observed. 相似文献
4.
Productive infection and cell-free transmission of human T-cell leukemia virus in a nonlymphoid cell line 总被引：34，自引：0，他引：34
P Clapham K Nagy R Cheingsong-Popov M Exley R A Weiss 《Science (New York, N.Y.)》1983,222(4628):1125-1127
Human T-cell leukemia virus (HTLV), American PL isolate, was transmitted by cocultivation and by cell-free filtrates to a nonlymphoid human osteogenic sarcoma (HOS) cell line, designated HOS/PL, but not to nine other lines bearing receptors for HTLV. HOS and HOS/PL cells are not dependent on interleukin-2 and do not express interleukin-2 receptors that are recognized by anti-Tac monoclonal antibody. HTLV released by the Japanese MT2 cell line was also transmitted to HOS cells. The infected HOS cells release substantial titers of progeny HTLV which is antigenically indistinguishable from parental virus and is able to transform T cells. 相似文献
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Near-infrared reflectance spectroscopy (NIRS) was evaluated as a possible alternative to gas chromatography (GC) for the quantitative analysis of fatty acids in forages. Herbage samples from 11 greenhouse-grown forage species (grasses, legumes, and forbs) were collected at three stages of growth. Samples were freeze-dried, ground, and analyzed by GC and NIRS techniques. Half of the 195 samples were used to develop an NIRS calibration file for each of eight fatty acids, with the remaining half used as a validation data set. Spectral data, collected over a wavelength range of 1100-2498 nm, were regressed against GC data to develop calibration equations for lauric (C12:0), myristic (C14:0), palmitic (C16:0), stearic (C18:0), palmitoleic (C16:1), oleic (C18:1), linoleic (C18:2), and alpha-linolenic (C18:3) acids. Calibration equations had high coefficients of determination for calibration (0.93-0.99) and cross-validation (0.89-0.98), and standard errors of calibration and cross-validation were < 20% of the respective means. Simple linear regressions of NIRS results against GC data for the validation data set had r2 values ranging from 0.86 to 0.97. Regression slopes for C12:0, C14:0, C16:0, C18:0, C16:1, C18:2, and C18:3 were not significantly different (P = 0.05) from 1.0. The regression slope for C18:1 was 1.1. The ratio of standard error of prediction to standard deviation was > 3.0 for all fatty acids except C12:0 (2.6) and C14:0 (2.9). Validation statistics indicate that NIRS has high prediction ability for fatty acids in forages. Calibration equations developed using data for all plant materials accurately predicted concentrations of C16:0, C18:2, and C18:3 in individual plant species. Accuracy of prediction was less, but acceptable, for fatty acids (C12:0, C14:0, C18:0, C16:1, and C18:1) that were less prevalent. 相似文献
7.
Scaglia G Swecker WS Fontenot JP Fiske D Fike JH Abaye AO Peterson PR Clapham W Hall JB 《Journal of animal science》2008,86(8):2032-2042
Small cow-calf operations are common in the Appalachian region. Tall fescue [Lolium arundinaceum (Schreb.) S. J. Darbyshire] is the dominant forage in these systems for direct grazing as well as for stockpiling. The present study was conducted from 2001 to 2005. A total of 108 Angus and Angus crossbred cows were allotted randomly to 6 forage systems and then to 3 replicates within each system. In brief, system 1 had a stocking rate of 0.91 ha/cow in a Middleburg 3-paddock (A, B, and C) system. System 2 was similar to system 1 except for a stocking rate of 0.71 ha/cow. A stocking rate of 0.71 ha/cow also was used in systems 3 through 6. All A paddocks had tall fescue, whereas B paddocks had tall fescue/white clover (Trifolium repens L.) except in system 6, which had tall fescue/lespedeza [Lespedeza cuneata (Dum. Cours.) G. Don]. System 3 evaluated a 2-paddock (A and B) rotational grazing system, and system 4 evaluated a 3-paddock (A, B, and C) rotational grazing system, with paddock C containing orchardgrass (Dactylis glomerata L.) and alfalfa (Medicago sativa L.). Systems 5 and 6 differed from system 2 in the areas of paddocks B and C as well as in the forage mixtures used. In paddock C, system 5 had switchgrass (Panicum virgatum L.) and system 6 had tall fescue and birdsfoot trefoil (Lotus corniculatus L.). System 1 had the greatest average herbage availability from weaning until breeding (P < 0.05) with the least amount of hay fed (P = 0.03) when compared with the remainder of the systems. Differences (P > 0.05) in percentage of ground cover were not detected among systems. There was no year x system interaction effect on the cow or calf performance variables evaluated and no treatment effect on cow performance variables. There was a treatment effect on calf performance variables. System 2 produced the greatest adjusted weaning weight, kilograms of calf weaned per hectare, and kilograms of calf per kilograms of cow at weaning (P < 0.05). Numerical ranking for total calf production per hectare from the greatest to least was system 2, 6, 3, 5, 4, and 1. Systems evaluated did not affect cow performance although differences in calf performance and overall productivity of the systems were observed. 相似文献
8.
Nguyen D. Kien Tran H. Quang Gunnar Jansson Chris Harwood David Clapham Sara von Arnold 《Annals of Forest Science》2009,66(7):711-711
9.
Tran Ho Quang Nguyen Duc Kien Sara von Arnold Gunnar Jansson Ha Huy Thinh David Clapham 《New Forests》2010,39(3):301-312
Lignin and cellulose contents and wood basic density were related to diameter at breast height (DBH) in six fast-growing and
five slow-growing families from a combined progeny test and seedling seed orchard of Eucalyptus urophylla grown for 10 years in northern Vietnam. The mean cellulose content of the fast-growing families was significantly higher
than that of the slow growing-families (40.0 and 37.1%, respectively), and for individual trees cellulose content was significantly
correlated phenotypically with DBH. Wood basic density was significantly lower in the fast-growing group than in the slow-growing
group (0.506 and 0.535 g cm−3, respectively), and was significantly negatively correlated phenotypically with DBH. The lignin contents were not significantly
different between groups. Cellulose content and wood basic density were not correlated. The main conclusion is that there
is no obstacle to combining high growth rate with high cellulose content, for plantation of forests intended mainly for pulpwood. 相似文献
10.
Clapham DE 《Science (New York, N.Y.)》2002,295(5563):2228-2229