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1.
Relationship between serum cortisol levels and some physiological parameters following reining training session in horse 
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下载免费PDF全文 Stefania Casella Irene Vazzana Elisabetta Giudice Francesco Fazio Giuseppe Piccione 《Animal Science Journal》2016,87(5):729-735
The changes of cortisol, red blood cells (RBC), hemoglobin (Hb), hematocrit (Hct), heart rate (HR), respiratory rate (RR) and rectal temperature (RT) were evaluated after a reining training session in eight Quarter Horses. All parameters were measured before exercise (T0), immediately after exercise (T1), 1 h after exercise (T2), 2 h after exercise (T3) and 24 h after exercise (T4). One‐way analysis of variance (ANOVA) for repeated measures, followed Bonferroni's post hoc test, showed a significant effect of the reining training session (P < 0.0001) on cortisol, RBC, Hb, Hct, HR, RR and RT. Simple linear regression analysis showed the positive correlation (P < 0.05) between cortisol changes and variations of studied parameters in T1, T3 and T4. Exercise‐induced cortisol concentrations reflect the physiological response of reining training, suggesting that the changes observed are useful to assess the performance in reining horses and their reining training adaptability. 相似文献
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Concetta Burgarella Miguel Navascués Álvaro Soto Ángel Lora Silvio Fici 《Annals of Forest Science》2007,64(7):757-763
In order to empirically assess the effect of actual seed sampling strategy on genetic diversity of holm oak (Quercus ilex) forestations in Sicily, we have analysed the genetic composition of two seedling lots (nursery stock and plantation) and their known natural seed origin stand by means of six nuclear microsatellite loci. Significant reduction in genetic diversity and significant difference in genetic composition of the seedling lots compared to the seed origin stand were detected. The female and the total effective number of parents were quantified by means of maternity assignment of seedlings and temporal changes in allele frequencies. Extremely low effective maternity numbers were estimated (Nf e ≈ 2–4) and estimates accounting for both seed and pollen donors gave also low values (N e ≈ 35–50). These values can be explained by an inappropriate forestry seed harvest strategy limited to a small number of spatially close trees. 相似文献
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Pectins from Foeniculum vulgare were extracted under acidic conditions. The obtained pectins were mainly composed of uronic acid but also contained traces of rhamnose, galactose, and arabinose. Extracted pectins were used as a carbohydrate source to prepare biopolymer films in the absence and in the presence of phaseolin protein. The swelling characteristics of the films were examined as a function of ionic strength, pH, and the applied osmotic stress. The swelling behavior was dominated by a Donnan-type effect, which decreases with increasing ionic strength and counterion valency. In all cases the swelling of films containing phaseolin was reduced, suggesting a network formation between protein and pectins. Mechanical property studies have also estimated the validity of the obtained novel biopolymer films in terms of mechanical resistance. 相似文献
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Mariniello L Giosafatto CV Di Pierro P Sorrentino A Porta R 《Journal of agricultural and food chemistry》2007,55(12):4717-4721
The ability of phaseolin to act as an acyl donor and acceptor substrate of transglutaminase was studied by using an enzyme isolated from Streptoverticillium mobarense. Phaseolin, a trimeric storage protein from Phaseolus vulgaris L., was shown to possess both glutamine and lysine residues reactive for the enzyme. The extent of transglutaminase-catalyzed cross-linking has been studied in function of both incubation time and enzyme concentration. Native- and SDS-PAGE demonstrated that phaseolin is intra- and intermolecularly cross-linked by transglutaminase and gives rise to different polymers as well as to modified forms of the protein having a similar molecular weight but lower Stokes radius if compared to unmodified phaseolin. Cross-linked phaseolin was found to be more resistant to proteolytic cleavage than the unmodified counterpart, as demonstrated by in vitro trypsin and pepsin digestion experiments. This behavior could suggest novel possible uses of the transglutaminase-modified phaseolin. 相似文献
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Gagnaire V Carpino S Pediliggieri C Jardin J Lortal S Licitra G 《Journal of agricultural and food chemistry》2011,59(23):12443-12452
Ragusano is a pasta filata cheese produced from raw milk in Sicily. The proteolysis was extensively analyzed after stretching (day 0), at 4 and 7 months of ripening through soluble nitrogen, urea-PAGE, and peptide identification by tandem mass spectrometry. After stretching, 123 peptides were identified: 72 arising from β-casein, 34 from α(s1)-casein, and 17 from α(s2)-casein. The main protein splitting corresponded to the action of plasmin, chymosin, cathepsin D, cell envelope proteinase, and peptidase activities of lactic acid bacteria. Unlike other types of cheeses, <10% residual β- and α(s)-caseins remained intact at 7 months, indicating original network organization based on large casein fragments. The number of identified soluble peptides also dramatically decreased after 4 and 7 months of ripening, to 47 and 25, respectively. Among them, bioactive peptides were found, that is, mineral carrier, antihypertensive, and immunomodulating peptides and phosphopeptides. 相似文献
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Marialuisa Menna Concetta Imperatore Filomena D’Aniello Anna Aiello 《Marine drugs》2013,11(5):1602-1643
Meroterpenes are widely distributed among marine organisms; they are particularly abundant within brown algae, but other important sources include microorganisms and invertebrates. In the present review the structures and bioactivities of meroterpenes from marine invertebrates, mainly sponges and tunicates, are summarized. More than 300 molecules, often complex and with unique skeletons originating from intra- and inter-molecular cyclizations, and/or rearrangements, are illustrated. The reported syntheses are mentioned. The issue of a potential microbial link to their biosynthesis is also shortly outlined. 相似文献
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Weak effects of habitat type on susceptibility to invasive freshwater species: an Italian case study 下载免费PDF全文
下载免费PDF全文 Angela Boggero Alberto Basset Martina Austoni Enrico Barbone Luca Bartolozzi Isabella Bertani Alessandro Campanaro Antonella Cattaneo Fabio Cianferoni Giuseppe Corriero Ambrosius Martin Dörr A. Concetta Elia Gentile Francesco Ficetola Lyudmila Kamburska Gianandrea La Porta Sara Lauceri Alessandro Ludovisi Elda Gaino Enzo Goretti Massimo Lorenzoni Marina Manca Aldo Marchetto Giuseppe Morabito Francesco Nonnis Marzano Alessandro Oggioni Cataldo Pierri Nicoletta Riccardi Giampaolo Rossetti Nicola Ungaro Pietro Volta Silvia Zaupa Diego Fontaneto 《水产资源保护:海洋与淡水生态系统》2014,24(6):841-852
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De Stefano C Milea D Porcino N Sammartano S 《Journal of agricultural and food chemistry》2006,54(4):1459-1466
As a contribution to understanding the speciation of mercury in the environment and to the study of the sequestering ability of phytate (Phy) toward heavy metal and organometal cations, this paper describes the results of an investigation (at t = 25 degrees C by potentiometry, ISE-H+ glass electrode) of its interactions with mercury(II) cation in NaCl aqueous solutions at different ionic strengths (I = 0.15 and 1.0 mol L(-1)), in the pH range 2.5 < or = pH < or = 9.5 and considering metal-to-ligand ratios of 1:1 < or = Hg/Phy < or = 4:1. The formation of 11 HgiHjPhy(12-2i-j)(- species with i = 1 and 0 < or = j < or = 7 and i = 2 and 0 < or = j < or = 2 was observed. Their complex formation constant values proved to be fairly dependent on ionic strength. The speciation of phytic acid and mercury(II) is also dependent on the metal-to-ligand ratio; the dependence of the stability of phytate-mercury(II) species on the phytate protonation step was modeled, and an empirical predictive relationship was proposed. From the results obtained, phytate has very good sequestering ability toward Hg2+, even in the presence of considerable excesses of chloride ion, that is, another ligand strongly interacting with mercury; this supports future studies both on the use of plants that naturally synthesize it for phytoremediation purposes and on its direct application in remediation techniques. 相似文献
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Antonia Concetta Elia Valentina Ciccotelli Nicole Pacini Ambrosius Josef Martin Dörr Marilena Gili Mauro Natali Laura Gasco Marino Prearo Maria Cesarina Abete 《Fish physiology and biochemistry》2014,40(4):1055-1068
Oxytetracycline (OTC) is employed in fish farms to contest or prevent bacterial infections. We simulated an OTC treatment at therapeutic level (75 mg kg?1) and at higher doses (150, 300 mg kg?1) for 10 days. A withdrawal period of 10 days was considered for treated carp, carrying out the same chemical and biochemical analyses (total glutathione, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase and malondialdehyde). The aim was to obtain data related to the carryover in muscle and on variations in the antioxidant indicators in liver and kidney. The OTC residual levels in muscle showed a dose–response relationship. After 10 days of treatment at the recommended dose (75 mg kg?1), the mean value in muscle was 295 μg kg?1. After 10 withdrawal days, residues in all treated groups were not entirely eliminated by fish. Residues of recommended 75 mg kg?1 OTC dose were lower than the maximum permitted by EEC regulation: 100 μg kg?1. Disturbance in the antioxidant systems in liver and kidney was recorded in (150, 300 mg kg?1) carp, as well as during the withdrawal period. A lowered superoxide dismutase activity and higher levels of catalase, glutathione peroxidase, glutathione reductase and glutathione were evaluated in liver, while in kidney only higher malondialdehyde and glutathione S-transferase concentrations were recorded for 300 mg kg?1 dose. The therapeutic OTC dose exerted lower effects, and only in liver, enhancement of GPx and GR activities was recorded. After the withdrawal period, altered antioxidant responses in tissues were restored for all three OTC doses. 相似文献