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1.
Parker M Pollnitz AP Cozzolino D Francis IL Herderich MJ 《Journal of agricultural and food chemistry》2007,55(15):5948-5955
2.
Cozzolino D Cynkar WU Dambergs RG Mercurio MD Smith PA 《Journal of agricultural and food chemistry》2008,56(17):7631-7636
Samples (n = 620) of homogenized red grape berries were analyzed using a visible and near-infrared (NIR) spectrophotometer (400-2500 nm) in reflectance. The spectra and the analytical data were used to develop partial least-squares calibrations to predict dry matter (DM) content and condensed tannins (CT) concentrations. The coefficient of determination in cross-validation and the standard error of cross-validation were 0.92 and 0.83% w/w for DM and 0.86 and 0.46 mg/g epicatechin equivalents for CT, respectively. The standard error in prediction was 1.34% w/w for DM and 0.89 mg/g epicatechin equivalents for CT, respectively. By implementing a NIR spectroscopy method to measure DM and CT in red grape homogenates, we have developed an approach that is suited to large-scale compositional analysis in commercial wine production facilities, as it enables the analysis of large numbers of samples needed to stream batches of fruit. From an economical point of view, the calibration models could be achieved with relatively small data sets. Thus, NIR offers a suitable and efficient tool for the simultaneous measurement of DM and CT in addition to other important parameters in red grape homogenates such as total anthocyanins, total soluble solids, and pH, with minimal sample preparation and low cost. 相似文献
3.
A. Chabirand E. Jouen O. Pruvost F. Chiroleu B. Hostachy M. Bergsma‐Vlami G. Bianchi L. Cozzolino J. Elphinstone M. Holeva F. Manole P. Martini H. Matoušková J. Minatchy G. Op de Beeck F. Poliakoff L. Sigillo F. Siverio J. Van Vaerenbergh M. Laurentie I. Robène‐Soustrade 《Plant pathology》2014,63(1):20-30
Efficient control of Xanthomonas axonopodis pv. dieffenbachiae, the causal agent of anthurium bacterial blight, requires sensitive and reliable diagnostic tools. The European standard EN ISO 16140:2003 has been followed to compare a nested PCR assay (N‐PCR) to a reference method (isolation and serological identification of bacterial colonies) and to other alternative serological detection methods. The evaluation was performed in two steps: a comparative study and a collaborative study involving 15 European laboratories. Although inclusivity was maximal (100%) for all methods, a maximal exclusivity was obtained only with N‐PCR followed by an enzymatic restriction digestion of the amplicons. Exclusivity indices of 90·6, 88·7 and 47·2% were found for indirect ELISA, immunofluorescence and double antibody sandwich ELISA, respectively. An exclusivity of 92·5% was obtained with the reference method, further increased to 100% if pathogenicity tests were performed as a supplemental assay. The best level of sensitivity (relative detection level) was obtained with the reference method followed by the N‐PCR assay. The N‐PCR performance in terms of relative accuracy, accordance and concordance was very similar to that of the reference method. Moreover, N‐PCR had undeniable advantages compared to the reference method (less labour‐intensive and less time‐consuming). In addition, post‐test probabilities of infection were calculated to select the most appropriate detection scheme related to the prevalence of the pathogen. The N‐PCR assay has since been included in a revised version of the EPPO detection protocol. 相似文献
4.
The use of near infrared (NIR) reflectance spectroscopy to measure the concentration of minerals and electric conductivity (EC) in red grape homogenates was investigated. Wine grape samples (n = 209) from two vintages, representing a wide range of varieties and regions were analysed by Inductively Coupled Plasma Optical Emission Spectrometry (ICPOES) for the concentrations of calcium (Ca), potassium (K), magnesium (Mg), phosphorus (P), sulphur (S), iron (Fe), and manganese (Mn) and scanned in reflectance in a NIR instrument (400-2500 nm). The spectra were pre-processed using multiple scatter correction (MSC) before developing the calibration models using partial least squares (PLS) regression and cross validation. Coefficients of determination in cross validation (R2) and the standard errors of cross validation (SECV) obtained were for Fe (0.60 and 1.49 mg kg−1), Mn (0.71 and 0.41 mg kg−1), Ca (0.75 and 60.89 mg kg−1), Mg (0.84 and 12.93 mg kg−1), K (0.78 and 285.34 mg kg−1), P (0.70 and 40.19 mg kg−1), S (0.88 and 14.45 mg kg−1) and EC (0.87 and 7.66 mS). The results showed that Mg, S and EC in grape berries might be measured by NIR reflectance spectroscopy. 相似文献
5.
Daniela Gargano Nunzia Scotti Alessandro Vezzi Alessandra Bilardi Giorgio Valle Stefania Grillo Salvatore Cozzolino Teodoro Cardi 《Genetic Resources and Crop Evolution》2012,59(3):419-430
The plastome sequence of the European cultivated potato, Solanum tuberosum subsp. tuberosum (tbr, GenBank accession no. DQ386163), was compared with that of S. bulbocastanum, a wild potato relative (blb, GenBank accession no. DQ347958), in order to characterize the degree and type of variability
in different genomic regions, and develop molecular markers relevant to genetics, breeding and biotechnology of potato. One
hundred forty-two and 251 PICs (Potentially Informative Characters) were found in coding and non-coding sequences (NCSs),
respectively. Further, while variation in coding regions was almost exclusively due to nucleotide substitutions, 25% of PICs
in NCSs of tbr and blb were due to indels, most of them mononucleotide or longer tandem repeats (micro and minisatellites).
Four intergenic regions were selected for further analyses in other 16 tuber-bearing Solanum species. The rps16-trnQ
UUG
gene spacer was found to be the most variable, forty-six PICs in this region distinguishing 18 haplotypes. Analysis of haplotype
relationships, based on variability in the four intergenic regions, confirmed that the most primitive species from Central
America were the most distant to S. tuberosum. Finally, polymorphic sites in the same regions were used to develop a set of CAPS (Cleaved Amplified Polymorphic Sequences)
markers for species/cytoplasm identification in Solanum spp. 相似文献
6.
Liu L Cozzolino D Cynkar WU Gishen M Colby CB 《Journal of agricultural and food chemistry》2006,54(18):6754-6759
Visible (vis) and near-infrared (NIR) spectroscopy combined with multivariate analysis was used to classify the geographical origin of commercial Tempranillo wines from Australia and Spain. Wines (n = 63) were scanned in the vis and NIR regions (400-2500 nm) in a monochromator instrument in transmission. Principal component analysis (PCA), discriminant partial least-squares discriminant analysis (PLS-DA) and linear discriminant analysis (LDA) based on PCA scores were used to classify Tempranillo wines according to their geographical origin. Full cross-validation (leave-one-out) was used as validation method when PCA and LDA classification models were developed. PLS-DA models correctly classified 100% and 84.7% of the Australian and Spanish Tempranillo wine samples, respectively. LDA calibration models correctly classified 72% of the Australian wines and 85% of the Spanish wines. These results demonstrate the potential use of vis and NIR spectroscopy, combined with chemometrics as a rapid method to classify Tempranillo wines accordingly to their geographical origin. 相似文献
7.
Pigna Massimo Cozzolino Vincenza Violante Antonio Meharg Andrew A. 《Water, air, and soil pollution》2009,199(1-4):371-379
Water, Air, & Soil Pollution - In the present paper, electrocoagulation (EC) has been employed for the reduction of chemical oxygen demand (COD) reduction of a distillery wastewater... 相似文献
8.
Bevin CJ Fergusson AJ Perry WB Janik LJ Cozzolino D 《Journal of agricultural and food chemistry》2006,54(26):9713-9718
This paper reports on the development of a rapid and simple method for red wine authenticity confirmation during transport and processing; namely, a wine "fingerprinting" system. When wine is transported between two sites, a sample is taken and a mid-infrared (MIR) spectrum is obtained. One hundred sixty-one (n = 161) samples of three main red wine varieties grown in Australia, Shiraz, Cabernet Sauvignon, and Merlot, were collected from six commercial wineries across Australia and scanned in transmission on two MIR spectrophotometers located at The Hardy Wine Company's main site at Reynella, South Australia (Foss WineScan FT 120) (926-5012 cm-1). A similarity index (SI) method was used as a tool to classify wine samples on the basis of their spectral data. The results showed that high rates of classification were obtained when wine samples scanned in different instruments were analyzed. The SI has been proven to provide an acceptable measurement for authentication of red wine integrity during transportation. In five of the six winery data sets, the SI correctly classified 98% of the wines. It was also observed that less than 1% of wines were misclassified between the different wineries investigated. Further studies are needed in order to test the applicability of the SI in a commercial situation and to evaluate its potential as a rapid quality control tool for routine use to authenticate wine samples during transport. 相似文献
9.
Fudge AL Wilkinson KL Ristic R Cozzolino D 《Journal of agricultural and food chemistry》2012,60(1):52-59
In this study, the suitability of mid-infrared (MIR) spectroscopy, combined with principal component analysis (PCA) and linear discriminant analysis (LDA), was evaluated as a rapid analytical technique to identify smoke tainted wines. Control (i.e., unsmoked) and smoke-affected wines (260 in total) from experimental and commercial sources were analyzed by MIR spectroscopy and chemometrics. The concentrations of guaiacol and 4-methylguaiacol were also determined using gas chromatography-mass spectrometry (GC-MS), as markers of smoke taint. LDA models correctly classified 61% of control wines and 70% of smoke-affected wines. Classification rates were found to be influenced by the extent of smoke taint (based on GC-MS and informal sensory assessment), as well as qualitative differences in wine composition due to grape variety and oak maturation. Overall, the potential application of MIR spectroscopy combined with chemometrics as a rapid analytical technique for screening smoke-affected wines was demonstrated. 相似文献
10.
Usefulness of near-infrared reflectance (NIR) spectroscopy and chemometrics to discriminate fishmeal batches made with different fish species 总被引：1，自引：0，他引：1
Cozzolino D Chree A Scaife JR Murray I 《Journal of agricultural and food chemistry》2005,53(11):4459-4463
Near-infrared reflectance (NIR) spectroscopy combined with chemometrics was used to identify and authenticate fishmeal batches made with different fish species. Samples from a commercial fishmeal factory (n = 60) were scanned in the NIR region (1100-2500 nm) in a monochromator instrument in reflectance. Principal component analysis (PCA), dummy partial least-squares regression (DPLS), and linear discriminant analysis (LDA) based on PCA scores were used to identify the origin of fishmeal produced using different fish species. Cross-validation was used as validation method when classification models were developed. DPLS correctly classified 80 and 82% of the fishmeal samples. LDA calibration models correctly classified >80% of fishmeal samples according to fish species The results demonstrated the usefulness of NIR spectra combined with chemometrics as an objective and rapid method for the authentication and identification of fish species used to manufacture the fishmeal. 相似文献