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1.
Latifeh Abdellahi Fariba Iraji Anahita Mahmoudabadi Seyed Hossein Hejazi 《Iranian Biomedical Journal》2022,26(1):1
Leishmaniasis is caused by protozoan Leishmania parasites that are transmitted through female sandfly bites. The disease is predominantly endemic to the tropics and semi-tropics and has been reported in more than 98 countries. Due to the side effects of anti-Leishmania drugs and the emergence of drug-resistant isolates, there is currently no encouraging prospect of introducing an effective therapy for the disease. Hence, it seems that the key to disease control management is the introduction of an effective vaccine, particularly against its cutaneous form. Advances in understanding underlying immune mechanisms are feasibale using a variety of candidate antigens, including attenuated live parasites, crude antigens, pure or recombinant Leishmania proteins, Leishmania genes encoding protective proteins, as well as immune system activators from the saliva of parasite vectors. However, there is still no vaccine against different types of human leishmaniasis. In this study, we review the works conducted or being performed in this field. Key Words: Immune response, Leishmaniasis, Vaccination 相似文献
2.
Samira Pirmanesh Rouha Kasra Kermanshahi Sara Gharavi Elahe Mobarak Qamsari 《Iranian Biomedical Journal》2022,26(2):153
Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular identification of lipase-producing Lactobacilli was performed by sequencing the 16Sr DNA gene, and a phylogenetic tree was constructed. The LAF_RS05195 gene, encoding lipase protein in L. fermentum isolates, was identified using specific primers, amplified by PCR (918 bp) and cloned into the pET28a (+) vector. The recombinant proteins were expressed 2, 4, 6, and 12 hours after induction with IPTG and assessed using the SDS-PAGE. Enzymatic activity of the purified recombinant protein was measured at 410 nm in the presence of ρ-NPA and ρ-NPP. Results:Among five identified native lipase-producing isolates, one isolate showed 98% similarity with Enterococcus species. The other four isolates indicated 98% similarity to L. fermentum. After purification steps with Ni-NTA column, a single protein band of about 34 kDa was detected on SDS- PAGE gel. The enzymatic activity of purified recombinant protein alongside ρ-NPA and ρ-NPP was measured to be 0.6 U/ml and 0.2 U/ml, respectively. Conclusion:In the present research, a novel lipase/esterase from L. fermentum was cloned and expressed. The novel lipase/esterase has the merit to be further studied due to its substrate specificity. Key Words: Escherichia coli, Gene expression, Lactobacillus, Lipase, Phylogeny 相似文献
3.
Soil salinity is becoming an increasingly serious constraint to plant growth in many parts of the world;this is particularly common in semi-arid and arid zones. This study was conducted to evaluate the... 相似文献
4.
Mazloom Z Hejazi N Dabbaghmanesh MH Tabatabaei HR Ahmadi A Ansar H 《Pakistan journal of biological sciences: PJBS》2011,14(19):900-904
Diabetes mellitus is one of the most wide spread endocrine disorders and an important developing health problem in the world. Cardiovascular disease is a common complication of type 2 diabetes. Several risk factors for coronary heart disease cosegregate in type 2 diabetes, including hyperglycemia, hyperlipaemia, increases production of free radical and decrease in antioxidant defense system. In this study we evaluated the effect of vitamin C supplementation on fasting and postprandial oxidative stress and lipid profile in type 2 diabetic patients. 30 patients with type 2 diabetes from Nader Kazemi Clinic, Shiraz, Iran were randomly divided into 2 groups; vitamin C treatment group (1000 mg d(-1)) and placebo group from May to September 2010. Fasting and postprandial lipid profile and Malondialdehyde (MDA) level were measured at the beginning of the study and after six weeks of supplementation. Data analysis was carried out using Mann-Whitney U test with p < 0.05 being significant by SPSS software version 16.The result of the study showed a significantly decrease in fasting (p = 0.006) and postprandial MDA (p < 0.001) in vitamin C group compare to placebo group but not in lipid profile. This study suggests that vitamin C supplementation can decrease fasting and postprandial oxidative stress and may prevent diabetes complication. 相似文献
5.
BACKGROUND: Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Delta) in induction of B7.1 and IL-2 molecules was studied. METHODS: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells and inducing the expression of the gene of interest. The expression of B7.1 molecule on the surface of the cells was assayed upon infection with Ad-E1Delta vector. Adenovirus-IL-2/B7.1 vector capable of inducing IL-2 and B7.1 expression in the cells was used as the positive control vector. RESULTS: According to the FACS results, about 4.17% of normal cells expressed B7.1 on their surface, while this level was increased in Ad-E1Delta transduced cells up to 14.43%. These results demonstrate that Ad-E1Delta vector considerably (about 3 folds) increases the expression of B7.1 on the cells. No detectable IL-2 was secreted into the medium of non-transduced and Ad-E1Delta transduced cells. CONCLUSION: Data indicate that the infection of C1498 cells with recombinant adenoviruses stimulates expression of B7.1 on the cell surface rather than secretion of IL-2 into the medium. 相似文献
6.
This paper presents a method to separate harvested area and yield for irrigated crops from rainfed crops in a region, given
gross harvested area and yield, and climatic, agronomic and economic data for crops. The method is based on the principle
of general maximum entropy, which combines incomplete data, empirical knowledge and a priori information to derive desired
information. The model is applied to three large basins with aggregated climatic and agricultural conditions, and to five
counties in Texas and California. The modeled results and assessed values in these study areas are compared. While the dependability
of model outputs relies on empirical knowledge and judicious parameter estimation, the model remains reliable even for the
significant level of uncertainty produced by subjectively predetermined major parameters. The model can be applied to retriving
historical data for irrigated and rainfed crops; it can also be used for irrigated and rainfed agriculture planning based
on climatic and technological projections. Moreover, the model provides other useful information, including water allocation
by crop, water use efficiency and the impact of other agricultural inputs. 相似文献
7.
Effect of different seed treatments on dormancy breaking and germination in three species in arid and semi-arid lands 总被引：1，自引：0，他引：1
Seeds of many woody plant species have one of several types of dormancy. They do not germinate unless specific environmental signals are in place or events occur. This study was conducted to evaluate the effects of scarification treatments on seed dormancy and germination of Acacia nilotica (L.) Willd. ex Del., Prosopis juliflora (Sw.) DC. and Dodonaea viscosa (L.) Jacq. The following treatments were applied: T1, untreated seed (control); T2, sulphuric acid (97%) for 45 min; T3, boiling water for 5 min. The seeds were cultured on a Murashige and Skoog (MS) medium after sterilization. The responses of seeds to treatments were compared with each other and with the control treatment. Germination was observed daily for a 30-day period. Results indicated positive responses to treatments, while impermeable seed coats may be responsible for low germination rates in intact seeds as seen experimentally in the untreated control. The highest germination was obtained for P. juliflora and D. viscosa acid-scarified seeds (80.8%-90.8%) and for scarified seeds of A. nilotica (50.2%) boiled in water. The germination indices, i.e., final germination percentage (FG), mean daily germination (MDG) and germination rate (GR), were significantly affected by treatments and species (p<0.01). 相似文献
8.
Azizi S Tajbakhsh E Fathi F Oryan A Momtaz H Goodarzi M 《Tropical animal health and production》2012,44(1):113-118
Maedi-visna (MV) is an important slow viral disease of sheep leading to a progressive lymphoproliferative disease. It affects
multiple organs primarily the lungs, where it causes interstitial pneumonia (maedi). In this study, the lungs of 1,000 sheep
carcasses were grossly inspected and those suspected to have maedi were studied at histopathological and molecular levels.
A polymerase chain reaction (PCR) technique that amplified a 291-base pair DNA in the long terminal repeat (LTR) sequence
of MV provirus was conducted on all the 50 suspected lungs together with 10 normal appearing lungs as controls. Amplicons
of the expected size were detected in 11 (n = 11/50) suspected sheep, and one of the 10 control sheep. Histopathologic study of the pulmonary lesions of all 11 (n = 11/11) positive sheep showed MV lesions, including hyperplasia of the perivascular and peribronchiolar lymphoid cells,
interstitial lymphoplasmacytic infiltration and smooth muscle hyperplasia and the histopathologic findings were correlated
with PCR results. In contrast, the tissue sections of control animals were almost normal at histopathological level; however,
PCR technique demonstrated that one of them was affected by maedi. This study showed that the LTR-PCR had high specificity
and sensitivity in diagnosis of this viral infection. This study is the first to evaluate the prevalence of MV virus infection
in sheep in Iran. 相似文献
9.
10.
Beigi Boroujeni M Beigi Boroujeni N Salehnia M Marandi E Beigi Boroujeni M 《Iranian Biomedical Journal》2012,16(1):33-37
Background: To achieve multiple oocytes for in vitro fertilization, ovulation induction is induced by gonadotropins; however, it has several effects on oocytes and embryo quality and endometrium receptivity. The aim of this study was to assess ultrastructural changes of corpus luteum after ovarian induction using human menopausal gonadotropin (HMG) and human chorionic gonadotropin (HCG) during luteal phase at implantation period. Methods: Female NMRI mice (6-8 weeks) were divided into control and stimulated groups. In the control group, the mice were rendered pseudopregnant and in the ovarian induction group, the mice were rendered pseudopregnant after the ovarian induction. The samples were obtained from the ovary in each group at the same time during luteal phase at implantation period. Ultrastructural changes were assessed using electron microscopy study. Results: Our results displayed some identifiable changes in ultrastructure of corpus luteum in ovarian induction group. These changes included enhancement of the apoptosis and intercellular space, whereas the angiogenesis was decreased. The findings indicated a decline in organelle density in the cytoplasm of ovarian induction, such as mitochondria, endoplasmic reticulum and polyribosome. Furthermore, chromatin condensation of nuclei was observed in some cells. Conclusion: The ovarian induction using HMG and HCG resulted in some ultrastructural changes on the corpus luteum at implantation period, which could affect on the pregnancy rate.Key Words: Corpus luteum, Luteal phase, Ultrastructure, Ovarian induction 相似文献