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Valentina Gonzlez María Jos Vargas-Straube Walter O. Beys-da-Silva Luclia Santi Pedro Valencia Fabrizio Beltrametti Beatriz Cmara 《Marine drugs》2020,18(11)
Marine actinobacteria are viewed as a promising source of enzymes with potential technological applications. They contribute to the turnover of complex biopolymers, such as pectin, lignocellulose, chitin, and keratin, being able to secrete a wide variety of extracellular enzymes. Among these, keratinases are a valuable alternative for recycling keratin-rich waste, which is generated in large quantities by the poultry industry. In this work, we explored the biocatalytic potential of 75 marine-derived actinobacterial strains, focusing mainly on the search for keratinases. A major part of the strains secreted industrially important enzymes, such as proteases, lipases, cellulases, amylases, and keratinases. Among these, we identified two streptomycete strains that presented great potential for recycling keratin wastes—Streptomyces sp. CHA1 and Streptomyces sp. G11C. Substrate concentration, incubation temperature, and, to a lesser extent, inoculum size were found to be important parameters that influenced the production of keratinolytic enzymes in both strains. In addition, proteomic analysis of culture broths from Streptomyces sp. G11C on turkey feathers showed a high abundance and diversity of peptidases, belonging mainly to the serine and metallo-superfamilies. Two proteases from families S08 and M06 were highly expressed. These results contributed to elucidate the mechanism of keratin degradation mediated by streptomycetes. 相似文献
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Bianca Ferreira Olivieri Camila Urbano Braz Fernando Brito Lopes Elisa Peripolli Rafael Medeiros de Oliveira Silva Rosana Ruegger Pereira da Silva Corte Lucia Galvo de Albuquerque Anglica Simone Cravo Pereira Nedenia Bonvino Stafuzza Fernando Baldi 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2021,138(1):80-90
The aim of this study was to identify differentially expressed genes (DEG) in the Longissimus thoracis muscle of Nelore cattle related to fatty acid (FA) profile through RNA sequencing and principal component analysis (PCA). Two groups of 10 animals each were selected containing PC1 and PC2 extreme DEG values (HIGH × LOW) for each FA group. The intramuscular fat (IMF) was compared between cluster groups by ANOVA, and only the sum of monounsaturated FA (MUFA) and ω3 showed significant differences (p < .05). Interestingly, the highest percentage (95%) of phenotypic variation explained by the sum of the first two PC was observed for ω3, which also displayed the lowest number of DEG (n = 1). The lowest percentage (59%) was observed for MUFA, which also revealed the largest number of DEG (n = 66). Since only MUFA and ω3 exhibited significant differences between cluster groups, we can conclude that the differences observed for the remaining groups are not due to the percentage of IMF. Several genes that have been previously associated with meat quality and FA traits were identified as DEG in this study. The functional analysis revealed one KEGG pathway and eight GO terms as significant (p < .05), in which we highlighted the purine metabolism, glycolytic process, adenosine triphosphate binding and bone development. These results strongly contribute to the knowledge of the biological mechanisms involved in meat FA profile of Nelore cattle. 相似文献
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Effect of farm characteristics and practices on hygienic quality of ovine raw milk used for artisan cheese production in central Italy 下载免费PDF全文
Elisa Carloni Annalisa Petruzzelli Giulia Amagliani Giorgio Brandi Francesco Caverni Piermario Mangili Franco Tonucci 《Animal Science Journal》2016,87(4):591-599
A survey on ovine dairy farms directly transforming own‐produced milk, in the Italian Marche region, was carried out to assess flock and milking practices that may influence milk hygienic‐sanitary conditions. A census survey established that 24 dairy farms were located in this region. Bulk milk samples were collected throughout the milking period in each dairy farm in 2013. Analyzed variables were: (i) chemical parameters such as fat, protein and lactose content, dry matter and pH; and (ii) total bacterial (TBC) and somatic cell counts (SCC). Chemical parameter values were in agreement with published data while, geometric mean (GM) log10 SCC was 5.91 and TBC GM was 57 978 colony forming units/mL, in compliance with Eropean Union criteria. A positive correlation was found between SCC and TBC when GMs of all farm data were considered (Spearman's rho = 0.7925; P = 0.0001). Statistical analysis did not show significant correlation between SCC or TBC GM and dairy farm principal characteristics. Although SCC levels detected in the present study should suggest the need to implement mastitis control programs, Marche's dairy sheep flocks revealed a good hygienic condition level. This is an important aspect in implementing safety for end users of the final product. 相似文献
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Detection of bacterial contamination and DNA quantification in stored blood units in 2 veterinary hospital blood banks 下载免费PDF全文
Valentina Stefanetti Arianna Miglio Katia Cappelli Stefano Capomaccio Elisa Sgariglia Maria L. Marenzoni Maria T. Antognoni Mauro Coletti Vittorio Mangili Fabrizio Passamonti 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(3):406-410
Blood transfusions in veterinary medicine have become increasingly more common and are now an integral part of lifesaving and advanced treatment in small and large animals. Important risks associated with transfusion of blood products include the transmission of various infectious diseases. Several guidelines suggest what infectious agents to screen for in canine and feline transfusion medicine. However, while the risk of bacterial contamination of blood products during storage and administration has not been documented in veterinary medicine, it has emerged as a cause of morbidity and mortality in human transfusion medicine. Clinical experience shows that the majority of blood component bacterial contaminations are caused by only a few species. Unlike other types of bacteria, psychrotolerant species like Pseudomonas spp. and Serratia spp. can proliferate during the storage of blood units at 4°C from a very low titer at the time of blood collection to a clinically significant level (> 105 CFU/mL) causing clinical sepsis resulting from red blood cell concentrate transfusions in human medicine. The purpose of this report was to describe the detection and quantification procedures applied in 4 cases of bacterial contamination of canine and feline blood units, which suggest the need for further investigations to optimize patients’ safety in veterinary transfusion medicine. 相似文献
6.
Cecilia Mónica Galosi Viviana Cid de la Paz Leandro Carlos Fernández Javier Pablo Martinez María Isabel Craig María Barrandeguy María Elisa Etcheverrrigaray 《Journal of veterinary diagnostic investigation》2005,17(5):500-502
This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion. 相似文献
7.
Narongrit Sombatsompop Nongyao Premkamolnetr Teerasak Markpin Santi Ittiritmeechai Chatree Wongkaew Wutthisit Yochai Preeyanuch Ratchatahirun Lee Inn Beng 《Asia Pacific viewpoint》2011,52(2):207-218
This opinion article expresses two key viewpoints regarding the options for Association of South‐East Asian Nations (ASEAN) universities in pursuit of fulfilling the demand for greater research visibility and academic reputation. The first viewpoint outlines the importance of promoting research collaboration among ASEAN researchers and their international peers in increasing the visibility and impact of ASEAN research. The second viewpoint examines the ASEAN research characteristics, the challenges and existing best practices of its journal publishing landscape, the potential role of non‐ASEAN international journals in improving the profile of ASEAN research and explores the needs and benefits of establishing an ASEAN Integrated Journal Publishing Network (IJPN). It is through these combined approaches, we believe, that effective policy change, the standardisation of publishing structure, procedures, and systems to drive journal development, improvement of journal quality, and establishment of a consistent framework for measuring researcher performance will result. This article also includes a proposal for the IJPN structure and implementation plan, and highlights the key benefits of taking a collaborative approach to achieving this common goal. 相似文献
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Stéphanie Mariette Emilie Balsemin Solenn Stoeckel Muriel Tavaud Hervé Le Bouler Frédérique Santi Michel Verger 《Annals of Forest Science》2007,64(5):533-539
Parents and progenies genetic diversity, and male and female contributions to the seed crop were assessed in three experimental Prunus avium seed orchards. Collected data were used to compare different effective population sizes, based on phenological, seed crop and paternity analysis. Our results did not show any difference of genetic diversity between parents and progenies. A limited pollen pollution was detected. We showed that distance and coflowering among clones had a significant effect on effective pollination, and a significant effect of the production of flowers was revealed in one of the seed orchards. Our study also revealed a quite low number of effective size of fathers per mother, but high effective sizes of mothers, fathers and parents at the level of the seed orchard. Finally, the calculation of effective size of mothers, fathers and parents was not highly modified when having the complete information based on the paternity analysis. 相似文献
10.
Karine Gendron Christine Goepfert Elisa Linon Horst Posthaus Caroline F. Frey 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(3):267-271
A young adult Labrador retriever dog was presented for surgical debulking of hepatic alveolar echinococcosis. Computed tomography detected hepatomegaly with multiple large cavitary masses with extension of tissue from a lesion wall into the caudal vena cava and numerous nodules in all lung lobes. Following euthanasia, histology confirmed parasitic vesicles with granulomatous reaction in all lesions, and polymerase chain reaction (PCR) established the causative agent to be Echinococcus multilocularis. This report is the first to present imaging features of pulmonary E. multilocularis granulomata in a dog. 相似文献