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Introduction It is well known fact that in some plants asparagine is the principal amide, and in others, glutamine. The environmental conditions, in which free amides are formed in the body of living crops, seemed to be divided roughly into two main parts as follows: First, nitrogen nutrition was supplied to the crop during the development. Second, the crop was subjected to physiological hydrolysis, for instance, placed in the darkness for the germination of the seed. 相似文献
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A condition similar to egg-drop syndrome-1976 (EDS-76) occurred in 14 broiler breeding flocks in 2 farms in Japan from December 1978 to January 1980, and it was diagnosed as EDS-76 by serologic and virological investigations. Egg production fell suddenly when the hens were 30 to 55 weeks of age, and the depression lasted 3 to 7 weeks. Production fell 6 to 25%. Depressed egg production was accompanied by the laying of shell-less, soft-shelled, and thin-shelled eggs associated with loss of egg-shell pigment. Eleven isolates of hemagglutinating adenovirus were isolated from cloacal swabs (10 isolates) and a uterus (1 isolate) of hens in one farm. One isolate, cloned and named JPA-1, had the same antigenicity in serologic tests and the same biological and physicochemical properties as the BC14 strain of EDS-76 virus. 相似文献
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Adenosine deaminase activity in six soil samples was measured. One g of moist soil was incubated with 0.02 M adenosine in 0.1 M phosphate buffer (pH 7.6) and toluene in a shaking water bath (30°C) for 72 h. Inosine and NH2 + were detected in the filtrate as degradation products of adenosine; inosine was identified by paper chromatography, and NH2 + was determined by Nessler’s method. The adenosine deaminase activity in soils was completely inhibited by autoclaving (120°C, 15 min). The relation between substrate concentration and reaction rate followed the Michaelis-Menten equation. The optimum pH for adenosine deaminase activity in soils ranged from about 7 to 8. The adenosine deaminase activity in soils ranged from about 1.51 to 4.33 munit (nmol per min) per g of dry soil at 30°C. 相似文献
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Hiroshi Doguchi Masanao Saio Shimpei Kuniyoshi Akiko Matsuzaki Naoki Yoshimi 《Journal of toxicologic pathology》2014,27(1):67-72
The effects of hyperbaric oxygen (HBO) on mouse skin two-stage chemical carcinogenesis were examined. Six-week-old inbred CD-1 female mice were divided into the following five groups: group 1, normoxia and application of 25 nmol 7,12-dimethylbenz[a]anthracene (DMBA) and 8.5 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA) (n=19); group 2, HBO and DMBA/TPA (n=21); group 3, HBO and DMBA/acetone (n=3); group 4, normoxia and acetone (n=3); and group 5, non-treatment group (n=5). HBO was started at the same time as DMBA. Mice were euthanized at 23 weeks after the start of the experiment. Mice in group 2 showed the occurrence of tumors at 8 weeks after the beginning of the experiment, while the occurrence of tumors in mice in group 1 was observed beginning at 9 weeks. There was a difference in occurrence among low-grade papillomas, high-grade papillomas and SCCs in both groups 1 and 2 by the χ2-test at end of the experiment (p<0.05). The Ki-67 labeling indices of tumors revealed that the percentages of positive cells in low-grade papillomas in groups 1 and 2 were 15.27 ± 2.54% and 29.67 ± 2.82%, respectively (p<0.01). The results suggested that the tumors in group 2, which was treated with HBO, were more progressive than those in group 1, which was not treated with HBO. In this study, HBO accelerated tumor cell proliferation and advanced tumor progression in skin carcinogenesis by DMBA/TPA. 相似文献
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