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Bovine babesiasis: its diagnosis and control   总被引:1,自引:0,他引:1  
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A 4‐year study (May 1997–December 2000) of the reproductive biology of pink dentex Dentex gibbosus (Rafinesque) from the Adriatic Sea revealed that this species is a rudimentary hermaphrodite. The smallest mature males and females captured were 38.70 cm in total length (TL) and 39.80 cm in total length (TL) respectively. The von Bertalanffy growth parameters estimated for the entire population were: L=107.24, K=0.12 and t0=−0.90. Fifty per cent of the population were sexually mature at 41.50 cm TL, while 100% of the specimens were sexually mature at 57.00 cm TL. Both monthly gonadosomatic index and macroscopically determined gonad stages strongly indicate that the pink dentex from the Adriatic Sea spawn partially in August, September and October. The mean value of absolute fecundity (F) was 1672 × 106 eggs. The results of great fertility of the pink dentex, partial spawning and relatively late sexual maturation suggest that the pink dentex has a high potential for commercial culture.  相似文献   
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Despite the importance of soil organic matter (SOM), very few long‐term data concerning soil organic‐C dynamics are available for calibrating and evaluating C models. The long‐term 14C‐turnover field experiment, established in 1967 in Fuchsenbigl, Lower Austria, offers the unique opportunity to follow the fate of labeled C under different crop‐management systems (bare fallow, spring wheat, crop rotation) over a period of more than 35 y. Compared with the crop‐rotation and spring‐wheat treatments, the decline of total organic C was largest in the bare‐fallow treatments, because no significant C input has occurred since 1967. Nonetheless, the decline was not as fast as predicted with the original RothC‐26.3‐model decomposition rate constants. In this work, we therefore calibrated the Roth‐C‐26.3 model for the Pannonian climatic region based on the field‐experiment results. The main adjustment was in the decomposition rate constant for the humified soil C pool (HUM), which was set to 0.009 instead of 0.02 y–1 as determined in the original Rothamsted field trial. This resulted in a higher HUM pool in the calibrated model because of a longer turnover period (111 vs. 50 y). The modeled output based on the calibrated model fitted better to measured values than output obtained with the original Roth‐C‐26.3‐model parameters. Additionally, the original decomposition rate constant for resistant plant material (RPM) was changed from 0.3 to 0.6 y–1 to describe the decomposition of 14C‐labeled straw more accurately. Application of the calibrated model (modified HUM decomposition rate) to simulate removal of crop residues showed that this can entail a long‐term decline of SOM. However, these impacts are strongly dependent on the crop types and on environmental conditions at a given location.  相似文献   
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Summary The chemotherapeutic efficacy of imidocarb dihydrochloride (3,3′-bis(2-imidazolin-2 yl) carbanilide dihydrochloride) administered as single intramuscular doses of 1·0, 2·0 and 2·5 mg/kg, against concurrent bovine anaplasmosis and babesiosis, is reported. Dosages of 2·0 and 2·5 mg/kg of imidocarb dihydrochloride rapidly inhibited acute ascending concurrent parasitaemias ofAnaplasma marginale, Babesia bigemina andBabesia argentina; however, 1·0 mg/kg had a minimal effect onA. marginale but was very effective againstB. bigemina andB. argentina. Imidocarb dihydrochloride at 1·0, 2·0 and 2·5 mg/kg inhibited the development of immunity of the acuteBabesia spp. infections, making the calves more susceptible to babesiosis upon challenge. The inhibition ofA. marginale parasitaemias was directly related to increasing doses of imidocarb dihydrochloride; however, recrudescing and persisting post-treatment parasitaemias also occurred more frequently at higher doses.
Sumario La eficiencia quemoterapéutica del imidocarb dihydrochloride (3,3′-bis (2-imidazolin-2 yl) carbanilide dihydro chloride) administrada como dosis intramuscular unica de 1·0, 2·0 y 2·5 mg/kg, en infecciones concurrentes de anaplasmosis y babesiosis es reportada en este trabajo. Dosis de 2·0 y 2·5 mg/kg de imidocarb dihydrochloride, rapidamente inhibieron las parasitemias concurrentes ascendientes y agudas deAnaplasma marginale, Babesia bigemina yBabesia argentina; sin embargo, 1·0 mg/kg tuvo un efecto minimo sobreA. marginale, pero fue muy efectivo contraB. bigemina yB. argentina. El imidocarb dihydrochloride en dosis de 1·0, 2·0 y 2·5 mg/kg inhibió el desarrollo de inmunidad contra la forma aguda deBabesia, haciendo a los terneros mas susceptibles a la babesiosis al desafio. La inhibición de parasitemias deA. marginale estuvo directamente relacionada al aumento de la dosis de imidocarb dihydrochloride; sin embargo, parasitemias debido al recrudecimiento ó a la persistencia despues del tratemiento, tambien ocurrieron mas frecuentemente en dosis altas.

Résumé L'efficacité de ce produit administré à l'occasion d'une unique injection à la dose de 1,0, 2,0 et 2,5 mg/kg contre l'anaplasmose et la babésiose simultanées est rapportée. La dose de 2 à 2,5 mg/kg inhibe rapidement le développement aigu de parasitémies dues à la fois àAnaplasma marginale, Babesia bigemina etBabesia argentina; cependant la dose de 1 mg/kg n'a qu'un effet modeste surA. marginale, alors qu'il conserve toute sa valeur contreB. bigemina etB. argentina. A la dose de 1,0, 2,0 et 2,5 mg/kg, ce corps entrave le développement de l'immunité dans les accés aigus dus àBabesia spp. rendant les veaux plus sensibles à de nouvelles attaques de babésioses. L'inhibition de parasitémie à base deA. marginale est directement en rapport avec l'accroissement de la dose d'Imidocarb dihydrochloride; toutefois, la recrudescence et la persistance après traitement de parasitémies sont plus fréquentes après usage de doses élevées.
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Many viruses have been identified in pericardial fluid and in tissue samples from humans with pericarditis by means of molecular diagnostics. In canine idiopathic pericardial effusion there is as yet no conclusive evidence to support the involvement of an infectious agent. This study was designed to investigate a possible relationship between idiopathic pericardial effusion in dogs and viruses most commonly encountered in humans affected with viral pericarditis. Coxsackievirus B3 RNA, influenza virus type A RNA, human adenovirus type 2 DNA, human cytomegalovirus DNA, and parvovirus B19 DNA were investigated using PCR on pericardial effusion samples and pericardial tissue specimens collected from 14 dogs with idiopathic pericardial effusion. PCR was also used to test for two bacteria, Borrelia burgdorferi and Chlamydia pneumoniae. The same microorganisms were also looked for in pericardial effusions or pericardial washes from 10 dogs with neoplastic pericardial effusion, and in samples collected from 10 dogs which died of a non-cardiac disease. One pericardial effusion sample from a dog with the idiopathic form of the disease tested positive for influenza virus type A and sequencing of the amplicon confirmed the PCR result. In another dog from the same group a cytomegalovirus was detected by PCR in the effusion, but sequencing showed this to be a false-positive result. The genomes of the microorganisms investigated were not detected in neoplastic effusions or pericardial washes. The results indicate that viral and bacterial DNA/RNA of relevance for human pericarditis is rare in pericardial samples from dogs with idiopathic pericardial effusion. The finding of influenza type A viral RNA in pericardial fluid from one dog with the idiopathic form of the disease warrants further investigation.  相似文献   
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Mortality of pear psylla to amitraz was studied by means of bioassays. Variation between samples, temporal variation within the season in one orchard and spatial variation between Swiss regions were considered. Variation between samples was large enough to produce different Probit functions and LC50 values. Temporal and spatial variations were too small to indicate resistance. Prediction intervals of the pooled functions using bootstrapping were calculated to determine if future samples come from a population with decreased sensitivity. Probabilistic criteria on the population level were proposed for resistance.  相似文献   
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Complement-fixation (CF) and indirect fluorescent antibody (IFA) antigens were prepared from Babesia bigemina isolates obtained in Texas. These serologic procedures were evaluated on 130 serum samples sequentially collected from 5 B bigemina-infected mature cattle, beginning on the day of exposure and continuing for 175 day thereafter. Both tests were effective in detecting specific antibodies for the first 84 days of infection, with 57 of 60 (95%) serums tested being positive on the CF test and 57 of 57 (100%) tests being positive to the IFA test. During the interval from 98 to 175 days, 24 of 60 (40%) of the serums tested were positive with the CF test, and 53 of 56 (95%) were positive with the IFA test. During the first 84 days, a similar linear regression occurred in both CF and IFA serum titers, but after 98 days the IFA regression flattened out, whereas the CF titers decreased below the sensitivity threshold in 60% of the serums tested.  相似文献   
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