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Comparative studies on three isolates of Breda virus of calves 总被引：8，自引：0，他引：8
G N Woode L J Saif M Quesada N J Winand J F Pohlenz N K Gourley 《American journal of veterinary research》1985,46(5):1003-1010
Three isolates of Breda virus of calves were compared morphologically and antigenically. The isolates demonstrated similar morphology and shared common antigens, as determined by enzyme-linked immunosorbent assay and immunoelectron microscopy. On the basis of results of the hemagglutination-inhibition test, enzyme-linked immunosorbent assay, and immunoelectron microscopy, the 3 isolates were further subdivided into 2 serotypes: serotype 1 (Breda virus 1) represented by the Iowa isolate 1; and serotype 2 (Breda virus 2), by the Ohio isolate and the Iowa isolate 2. The 3 isolates caused diarrhea in gnotobiotic calves. 相似文献
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Laparoscopic artificial insemination in sheep 总被引：2，自引：0，他引：2
The goal of any AI program is to create improved offspring, and the achievement of this objective will depend on the breeding value of the ram and ewe selected. Laparoscopic AI is being utilized in the sheep industry to extend the use of superior rams, and it offers the producer the opportunity to maximize the reproductive potential of superior sheep. Rapid genetic trait infusion of known superior stud rams into the flock is the primary economic benefit of laparoscopic AI. The success of laparoscopic AI depends on events and factors that interrelate in a complex way. Once the selection and preparation of the ewe have been accomplished, one of the more important steps in the program is the successful synchronization of the ewe to deliver the necessary ova to the site of fertilization at a specific time. One of the best methods of synchronization for laparoscopic AI is the use of a progesterone product for a controlled time period and the administration of PMSG upon its removal. Detecting the onset of estrus is critical, and the addition of sterile (e.g., vasectomized) males is helpful, even essential, to accurately determine when each ewe begins her estrus. The ram effect has been shown to stimulate ovulation and estrus. Ewes must be inseminated within a narrow window of time after the synchronization product is removed. Ewes should be inseminated in the order in which they begin to exhibit signs of behavioral estrus, but age, stage of lactation, duration of behavioral estrus, and breed must be taken into account when this order is established. Fresh-extended semen works well throughout this preferred time frame established for laparoscopic AI, but frozen semen gives best results when used near the end. Advancement in manufacturing technology today removes equipment as a variable factor. It is important, therefore, that the inseminator develop a level of expertise in laparoscopy to ensure maximum fertilization rates. If available, fresh-extended semen is preferred over frozen semen, using at least the minimal number of spermatozoa necessary for fertilization. Evaluation of the post-thaw frozen or fresh semen is necessary to determine motility, morphology, and concentration, all of which help determine the volume of the insemination dose. The minimum necessary for laparoscopic AI in fine-wooled breeds is 20 X 10(6) normal motile spermatozoa; however, the more seasonal and less fertile American sheep need approximately 40 to 50 X 10(6) normal motile sperm to achieve acceptable fertility rates.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
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C R Gregory I M Gourley J R Snyder J Ilkiw 《American journal of veterinary research》1992,53(1):153-156
Dissection, injection, and surgical studies in feline cadavers and in anesthetized cats were conducted to determine the feasibility of using the gracilis muscle as the basis for a free musculocutaneous flap. The vascular pedicle of the flap consisted of the femoral artery and vein. Mean length (1.6 +/- 0.2 cm) of the vascular pedicle and mean artery (1.33 +/- 0.19 mm) and vein (2.55 +/- 0.38 mm) diameters were satisfactory for microvascular transfer. Fluorometry revealed overlying cutaneous perfusion in the flaps on the basis of their muscle vascular pedicles. To ensure survival of the flap, the muscular branches of the femoral artery and vein supplying the gracilis muscle had to be carefully preserved during surgical elevation of the flap. 相似文献
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J Gourley 《The Journal of small animal practice》1966,7(1):33-61
Abstract— A comprehensive review of procedures for case recording, cash collection and accounts, leaflets for clients and notes to staff adopted in one practice. Examples are given of the procedures and leaflets etc., prepared in this and other practices. Résumé— Un compte-rendu fonctionnel des démarches pour l'enregistrement des cas, les encaissements et la comptabilité, prospectus pour les clients et mémorandum au personnel de chaque étude. Des exemples des démarches et des prospectus etc, préparés ont été donnés. Zusammenfassung— Ein umfassender Überblick über die Art der Aufzeichnungen von Krankengeschichten, Barund Kontenzahlungen, Merkblätter für Klienten und Personalanweisungen, wie sie in einer Praxis durchgeführt wurden. Beispiele dieser Aufzeichnungen sowie Merkblätter u.s.w sind angführt. 相似文献
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Hepatic dearterialization was performed in 9 adult dogs. Evaluation of the technique was based on clinical and laboratory assessment of physiologic and pathologic changes related to the procedure. All dogs had hematologic and histopathologic evidence of transient hepatic ischemia that was reflected by minimal post-operative morbidity. 相似文献
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C. R. Gregory DVM I. M. Gourley DVM PhD N. J. Taylor BS T. W. Broaddus DVM PhD R. B. Olds DVM J. D. Patz BS 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1987,1(2):53-60
Renal allograft transplantation was performed as treatment for terminal renal failure in six veterinary patients. Three patients failed to survive the postsurgical period, one patient died due to acute allograft rejection, one patient died subsequent to complications of previous parenteral hyperalimentation and infection, and one patient was euthanatized due to the inability of the clients to maintain the effort necessary to manage a transplant recipient. Further clinical studies are necessary to determine if renal transplantation with cyclosporine (cyclosporin A)/prednisolone immunosuppression has the potential to provide practical treatment of terminal renal disease in clinical veterinary medicine. Pharmacologic and immunologic monitoring of transplant recipients with individualized immunosuppression is imperative. Careful surveillance is necessary to identify and eliminate nosocomial sources of infection. Candidates for transplantation cannot be critically ill or malnourished prior to surgery, and pet owners must be prepared for the financial and time commitments associated with caring for a renal allograft recipient. 相似文献
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