Editorial     

C. Guillermo Couto 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(2):71-71

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Stem cell factor supports migration in canine mesenchymal stem cells     

Nathaly Enciso  Luciana L. K. Ostronoff  Guillermo Mejías  Leticia G. León  María Luisa Fermín  Elena Merino  Cristina Fragio  Luis Avedillo  Concepción Tejero 《Veterinary research communications》2018,42(1):29-38

Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC). We also analyzed the role of Stem Cell Factor (SCF) on MSC proliferation and on ASCs metalloproteinases MMP-2, MMP-9 secretion. Healthy dogs were used as BMMSC donors, and ASC were collected from omentum during elective ovariohysterectomy surgery. Both cell types were cultured in IMDM medium with or without SCF, 10% Dog Serum (DS), and incubated at 38 °C with 5% CO2. Growth of BMMSCs and ASCs was exponential until 25–30 days. Flow citometry of MSCs revealed positive results for CD90 and negative for CD34, CD45 and MCH-II. Genes were evaluated by RT-PCR and metalloproteinases by zymografy. Our findings indicate morphological and immunological similarities as well as expression of genes from both origins on analyzed cells. Furthermore, SCF did not affect proliferation of MSCs, however it up-regulated MMP-2 and MMP-9 secretion in ASCs. These results suggest that metalloproteinases are possibly essential molecules pivoting migration.  相似文献   

Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp. berkhoffii   总被引：1，自引：0，他引：1  

Diniz PP  Maggi RG  Schwartz DS  Cadenas MB  Bradley JM  Hegarty B  Breitschwerdt EB 《Veterinary research》2007,38(5):697-710

The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the S?o Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% (4/197) and 1.5% (3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer (ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples (n=8) were inoculated into a liquid pre-enrichment growth medium (BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog (ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii (pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs.  相似文献   

Effect of Density at Harvest on the Growth Performance and Profitability of Hatchery‐reared Spotted Rose Snapper,Lutjanus guttatus,Cultured in Floating Net Cages          下载免费PDF全文

Carlos Humberto Hernández  Crisantema Hernández  Francisco Javier Martínez‐Cordero  Nicolás Castañeda‐Lomas  Guillermo Rodríguez‐Domínguez  Albert G. J. Tacon  Eugenio Alberto Aragón‐Noriega 《Journal of the World Aquaculture Society》2016,47(1):51-60

This study evaluated the effect of the density at harvest on the performance and profitability of hatchery‐reared spotted rose snapper cultured in cages. The fish were stocked at harvest densities of 15, 20, and 22 kg/m³ in cages of 222 and 286 m³. More than 39,000 snapper fingerlings with an initial weight of 14 g were stocked. The fish were fed an extruded diet and cultured over a 360 d period. The thermal growth coefficient ranged from 0.04 to 0.05 and survival was 95% for all treatments, with the highest final weight (436.8 g) observed for fish reared at a density of 20 kg/m³. The allometric value b indicated that hatchery‐raised, cage‐cultured snapper were heavier than their wild counterparts. The major costs were feed (ranging from 44.7–45.9%), labor (22.4–32.6%), and seed costs (20.2–26.1%). The total production cost ranged from US$ 6.5 to US$ 7.5/kg. The baseline scenario was not economically feasible. However, a 10% increase in the sales price resulted in increases in the internal rate of return (183%) and net present value (US$ 97,628.9). These results suggest that L. guttatus has the potential for commercial production in cages.  相似文献   

Synthesis and toxicological properties of some alkyl and aryl 3,4-methylenedioxyphenyl N,N′-thio-bis-N-methylcarbamates     

Guillermo C. Wallace  Eduardo N. Zerba 《Pest management science》1989,26(3):215-225

A series of asymmetrical thio-bis-N-methylcarbamate derivatives were synthesised by combining 3,4-methylenedioxyphenyl N-methylcarbamate with different N-methylcarbamates. Some of these derivatives combined good insecticidal activity against the housefly with reduced acute oral toxicity to mice. The thio-bis-N-methylcarbamates obtained exhibited a lower anti-cholinesterase activity than the more toxic of the two carbamate moieties contained in each combination. The weak synergism of piperonyl butoxide on the insecticidal activity of the derivatives suggests the possibility of a self synergism phenomenon caused by the 3,4-methylenedioxyphenyl group.  相似文献   

What is new on feline lymphoma?     

Guillermo Couto C 《Journal of Feline Medicine and Surgery》2001,3(4):171-176

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CORNEAL ULCERS IN THE DOG     

Virginia P.  Studdert B.S.  D.V.M. 《Australian veterinary journal》1967,43(10):466-470

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Polymerase chain reaction tests for the identification of Ross River, Kunjin and Murray Valley encephalitis virus infections in horses   总被引：1，自引：0，他引：1  

Studdert MJ  Azuolas JK  Vasey JR  Hall RA  Ficorilli N  Huang JA 《Australian veterinary journal》2003,81(1-2):76-80

OBJECTIVE: To develop and validate specific, sensitive and rapid diagnostic tests using RT-PCR for the detection of Ross River virus (RRV), Kunjin virus (KV) and Murray Valley encephalitis virus (MVEV) infections in horses. METHODS: Primer sets based on nucleotide sequence encoding the envelope glycoprotein E2 of RRV and on the nonstructural protein 5 (NS5) of KV and MVEV were designed and used in single round PCRs to test for the respective viruses in infected cell cultures and, in the case of RRV, in samples of horse blood and synovial fluid. RESULTS: The primer pairs designed for each of the three viruses amplified a product of expected size from prototype viruses that were grown in cell culture. The identity of each of the products was confirmed by nucleotide sequencing indicating that in the context used the RT-PCRs were specific. RRV was detected in serums from 8 horses for which there were clinical signs consistent with RRV infection such that an acute-phase serum sample was taken and submitted for RRV serology testing. The RRV RT-PCR was analytically sensitive in that it was estimated to detect as little as 50 TCID50 of RRV per mL of serum and was specific in that the primer pairs did not amplify other products from the 8 serum samples. The RRV primers also detected virus in three independent mosquito pools known to contain RRV by virus isolation in cell culture. Samples from horses suspected to be infected with KV and MVEV were not available. CONCLUSION: Despite much anecdotal and serological evidence for infection of horses with RRV actual infection and associated clinical disease are infrequently confirmed. The availability of a specific and analytically sensitive RT-PCR for the detection of RRV provides additional opportunities to confirm the presence of this virus in clinical samples. The RT-PCR primers for the diagnosis of KV and MVEV infections were shown to be specific for cell culture grown viruses but the further validation of these tests requires the availability of appropriate clinical samples from infected horses.  相似文献   

Fertility and sperm quality of broiler breeder males infected with subgroup J avian leukosis virus     

Hudson BP  Wilson JL  Zavala G  Sander JE 《Avian diseases》2002,46(4):1033-1037

In order to assess the effects of subgroup J avian leukosis virus (ALV-J) on semen quality, broiler breeder males were separated by ALV-J status (ALV-J positive = POS, ALV-J negative = NEG) at 44 wk of age. Of the 249 males originally placed at 1 day of age, 101 (40.6%) died by 43 wk of age. Observations of tumor expression and high mortality suggest that many of the males that died prior to 44 wk of age were infected with ALV-J. From 47 to 56 wk of age, hens were inseminated every third week with 7.5 x 10(7) sperm. Fertility and hatch data were collected by incubating eggs laid during the 2 wk postinsemination (WPI). The number of sperm that penetrated the perivitelline membrane of the ovum was determined from eggs laid on the eighth day postinsemination. Sperm mobility index (SMI) was determined at 58 and 60 wk of age from all males producing semen. Whereas SMI and sperm hole penetration measurements indicated that the sperm quality from treatments POS and NEG were similar, fertility was significantly greater in the POS treatment during the first (89.0% vs. 79.0%) and second WPI (59.3% vs. 45.0%). However, because of numerically higher hatch of fertile from the NEG group, the percentage of hatch of eggs set was similar between groups. These data suggest that ALV-J status of caged males has no influence on sperm quality or hatchability of eggs.  相似文献   

Two new nematodes,Paraseuratoides ophisterni gen. et sp. n. (Quimperiidae) and Philometra ophisterni sp. n. (Philometridae), from the swamp-eel Ophisternon aenigmaticum in Mexico     

Moravec F  Salgado-Maldonado G  Aguilar-Aguilar R 《Folia parasitologica》2002,49(2):109-117

Two new nematodes, Paraseuratoides ophisterni gen. et sp. n. (Seuratoidea: Quimperiidae) and Philometra ophisterni sp. n. (Dracunculoidea: Philometridae) are described based on specimens recovered from the intestine and mesentery, respectively, of the swamp-eel Ophisternon aenigmaticum Rosen et Greenwood (Synbranchiformes: Synbranchidae) from a canal of the Papaloapan River in Tlacotalpan, State of Veracruz, Mexico. The genus Paraseuratoides is mot similar to Paraseuratum Johnston et Mawson, 1940, differing from it mainly in the absence of a bulbous inflation on the anterior end of the oesophagus and in the structure of the mouth (presence of 6 spines in addition to 6 oesophageal teeth). Neoquimperia Wang, Zhao, Wang et Zhang, 1979 and Wuinema Yu et Wang, 1992 are synonymised with Ezonema Boyce, 1971 and Paragendria Baylis, 1939, respectively, and Haplonema hamulatum Moulton, 1931 is considered a junior synonym of Ichthyobronema conoura Gnedina et Savina, 1930. Philometra ophisterni (only females) is mainly characterised by minute cephalic papillae, a greatly developed anterior oesophageal bulb separated from the cylindrical part of the oesophagus, anterior extension of the oesophageal gland anterior to the nerve ring, and by the character of large caudal projections. This is the first Philometra species recorded from inland fishes in Mexico.  相似文献