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Six-week-old Nile tilapia (Oreochromis niloticus) fry with an average weight (SD) of 0.51(0.2) g were reared for 140 days on five formulated, isocaloric diets of different protein levels (25, 30, 40 and 45% by dry weight). Fish fed diets of higher protein levels (40 and 45%) showed better growth and feed conversion ratio than those on lower protein levels. Fast-growing fish matured earlier. Maturation rate was affected by the dietary protein levels. Males matured earlier than females: the first mature males were recorded when they were 14 weeks old, whereas the females matured after 18 weeks. In both sexes, mean percentage of mature fish rose with increasing dietary protein level, the percentage of mature males being higher than that of the females. Similarly, the percentage of mature fish rose with the increasing age of fish, with more than 50% males and females mature at the age of 22 and 24 weeks, respectively, the exception being the 25% protein diet fed fish, where the percentage of mature fish was below 50%. In all treatments, spawning was initiated when the fish were 22 weeks old. Smallest size at spawning of males and females was 9.2 cm (13.1 g) and 8.1 cm (8.9 g), respectively, and dietary protein levels influenced the size of fish at first maturity. For both sexes, no difference was found in the gonado-somatic index (GSI) among different treatments. Fecundity increased with increasing dietary protein levels, but significant differences were found only between 40–45% and 25–35% dietary protein levels. The relative fecundity (eggs g–1 female) was higher at the lower dietary protein levels (25–35%) than at the higher dietary protein levels (40–45%). The dietary protein levels did not have any significant influence on the size and weight of mature eggs. The chemical composition of fish and mature ovaries was significantly influenced by the dietary protein level.  相似文献   
2.
Arbuscular mycorrhizal (AM) fungi are root symbionts that enhance plant growth and improve soil fertility and soil structure in drylands. Even though AM fungi are obligate biotrophs, organic matter (OM) can stimulate their growth, but the mechanisms behind this are still unknown. Here, we compared the effect of nutrient patches of different OM sources to intrinsic components of OM such as inorganic nutrient supply and an improved soil water-holding capacity (WHC; via application of hydrophilic polymers), on AM fungal growth. Fatty acids extracted from in-growth mesh bags incubated in the field were used as biomarkers for AM fungi and other soil microbes. We found an enhancement of AM fungal growth in certain nutrient patches. Two out of three OM types stimulated AM fungal growth strongly, and also the addition of inorganic nutrients enhanced AM fungi, though to a lesser extent than OM. Enhanced soil WHC, on the other hand, did not influence AM fungal growth. AMF were more strongly enhanced by the mineral nutrients relative to other soil organisms. Intrinsic nutrients might be an important factor for AMF growth stimulation in OM additions, but there was no evidence that nutrients alone can explain this phenomenon.  相似文献   
3.
The cocomposting of agricultural waste is a new management priority in Tunisia. In this study, four composts were evaluated by comparing the changes in measured hydrophysical and physicochemical properties and phytotoxicity. The organic wastes used were almond shell (AS), sesame bark (SB), olive husk (OH), and green and wood wastes (GW and WW, respectively). Composts CI and CII were composed of AS/SB and OH/SB, respectively, at a ratio of 75/25 (wet weight basis). CIII consisted of OH, SB and CAS (coarsely-ground almond shell used as a bulking agent) at a ratio of 55/25/20. Finally, CIV was composed of 25%SB+9%CAS+18%GW+48%WW. The composts studied were characterized by basic pH and an electric conductivity (EC) value ranging from 1.6 to 2.4 mS/cm. The organic matter contents (OM) and C/N ratios of composts ranged from 20 to 46% and from 10 to 21%, respectively. Based on hydrophysical analyses, composts CI, CIII and CIV, containing AS, were shown to have a porosity and a water content of 10-26% and 10-20%, respectively. The phytotoxicity of composts was studied on the basis of cress seed germination. Results revealed that differences in properties are mainly related to the nature of composted waste and that some of these composts are compatible for use as constituents in growing media for horticultural soilless cultures.  相似文献   
4.
The amounts of olive husks produced in Mediterranean countries are very significant. Their treatment and disposal are becoming serious environmental problems. Increasing attention has been paid to finding a use for olive husks. A technological treatment is available to reduce their pollutant effects and to transform them into valuable products. The most suitable procedures are recycling instead of the detoxification of these wastes. It is possible to transform olive husks into organic fertilizers (composts) by composting with poultry manure. The compost has no phytotoxicity and may improve soil fertility and plant production. The composting process involves the microbial degradation of the polluting load of the solid wastes. Results of experiments using olive husk composts in crop production have shown that yields obtained with organic fertilization are similar, and sometimes higher, than those obtained with classic manure. Their bioavailability may be linked to the soil humic complexes originated by these organic fertilizers and to mineral components (active lime and clay). The composition of vegetable leaves improved similitude between compost and manure, normal concentrations but with deficiency in nitrogen, phosphorus and potassium.  相似文献   
5.
The aim of this study was to investigate the antioxidant role of zinc (Zn) in the Cd-exposed testes of Wistar rats. Subchronic exposure to Cd (CdCl(2), 40 mg/l, per os) for 30 days resulted in a significant reduction in growth rate (-11%) and relative weights of testes (-36%) and seminal vesicles (-80%). Treated rats displayed a decrease in testicular and plasma testosterone levels, respectively (-70%, P<0.05; -48%, P<0.05), epididymal sperm count (-22%, P<0.05), and spermatozoa motility (-35%, P<0.05). In contrast, Cd increased the malondialdehyde (+46%, P<0.05), metallothionein (+200%, P<0.05), and 8-oxodGuo concentrations (+71%, P<0.05) in the testis. In the gonad, Cd decreased the GPx (-30%, P<0.05), CAT (-32%, P<0.05), mitochondrial Mn-SOD (-34%, P<0.05), and cytosolic CuZn-SOD (-32%, P<0.05) activities. Zinc supplementation (ZnCl(2), 40 mg/l, per os) in the Cd-exposed rats restored the activities of GPx, CuZn-SOD, and Mn-SOD in the testes to the levels of the control group. Moreover, zinc administration was capable of reducing the elevated levels of malondialdehyde in the testis. Interestingly, zinc supplementation attenuated DNA oxidation induced by Cd in the gonad and restored the testosterone level and sperm count to the levels of the control group. Zinc administration minimized oxidative damage and reversed the impairment of spermatogenesis and testosterone production induced by Cd in the rat testis.  相似文献   
6.
Severe toxicity was detected in mussels from Bizerte Lagoon (Northern Tunisia) using routine mouse bioassays for detecting diarrheic and paralytic toxins not associated to classical phytoplankton blooming. The atypical toxicity was characterized by rapid mouse death. The aim of the present work was to understand the basis of such toxicity. Bioassay-guided chromatographic separation and mass spectrometry were used to detect and characterize the fraction responsible for mussels’ toxicity. Only a C17-sphinganine analog mycotoxin (C17-SAMT), with a molecular mass of 287.289 Da, was found in contaminated shellfish. The doses of C17-SAMT that were lethal to 50% of mice were 750 and 150 μg/kg following intraperitoneal and intracerebroventricular injections, respectively, and 900 μg/kg following oral administration. The macroscopic general aspect of cultures and the morphological characteristics of the strains isolated from mussels revealed that the toxicity episodes were associated to the presence of marine microfungi (Fusarium sp., Aspergillus sp. and Trichoderma sp.) in contaminated samples. The major in vivo effect of C17-SAMT on the mouse neuromuscular system was a dose- and time-dependent decrease of compound muscle action potential amplitude and an increased excitability threshold. In vitro, C17-SAMT caused a dose- and time-dependent block of directly- and indirectly-elicited isometric contraction of isolated mouse hemidiaphragms.  相似文献   
7.
The effects of dietary protein (25%, 30%, 35%, 40% and 45%) on growth, survival, feed conversion ratio (FCR), protein efficiency ratio (PER) and body composition were investigated for four sizes (0.51, 45, 96 and 264 g) of Nile tilapia, Oreochromis niloticus L. In all four experiments, there was a progressive increase in growth with increasing dietary protein. In fry (0.51 g), significantly higher growth, survival and feed conversion were recorded for fish fed 40–45% rather than 25–35% protein diets. Similar trends for growth and FCR were also noted in 45 g fish. For larger (96 and 264 g) tilapia, significant differences in growth and FCR were found only between fish fed 25% and 30–45% protein diets. FCR and PER decreased with increasing weight of fish, and both were found to be negatively correlated with dietary protein level. Whole-body composition of the smallest fish was significantly influenced by dietary protein content. Percentage body protein of the fish fed 40–45% protein was higher than that of fish fed 25–35% protein diets, whereas lipid content decreased with increasing dietary protein level. In 45 g fish, both protein and lipid contents were higher in fish fed 25% and 30% protein diets than in those fed 35–45% protein diets. In larger tilapia, no significant influence of dietary protein level on body protein content was found. Percentage lipid decreased with increasing dietary protein level, and no definite trends in ash content were found. The results of these studies indicate that O. niloticus fry (0.51 g) should be reared on a practical diet containing 40% protein, and larger tilapia (96–264 g) on a diet containing 30% protein.  相似文献   
8.
The composting process of organic wastes consists of the bioconversion of biologically unstable wastes to stabilized products for application to soils. This study was carried out on different soils (brown isohumic soils in Sfax region in southern Tunisia and calcimagnesic soils in Korba region in northern Tunisia). The mineralization process of organic matter is more important in isohumic soils, showing less tenors of fine fractions than in calcimagnesic soils. The study of different humic fractions in amended soils shows that humic acid tenors and the polymerization rates are linked to soil nature. Calcimagnesic soils ensure a more important humification than isohumic soils.  相似文献   
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