Comparison of media to isolate Staphylococcus aureus from teat skin and milking unit liners.     

L K Fox  C T Gaskins  D D Hancock  D Newkirk  C T Hutton 《The Cornell veterinarian》1992,82(3):225-231

Four procedures were compared for isolation of Staphylococcus aureus from swabbing solutions of teat skin and milking unit liners from commercial dairies. In 2 procedures, 0.1 ml of swabbing solutions were added to either 5 ml Vogel-Johnson or Baird Parker broth media and enriched at 37 degrees C, 4 h. Following enrichment, 0.1 ml culture was transferred to modified Baird-Parker agar and incubated at 37 degrees C, 48 h. In the other 2 procedures, 0.1 ml of swabbing solution was directly placed on either blood or modified Baird-Parker agar plates and incubated at 37 degrees C 48 h. Combining results from all methods, Staphylococcus aureus were isolated from 72 of 913 (7.9%) skin samples, and 34 of 268 liners (12.6%). On average, 43.1% (31/72) of the S. aureus isolates were found by the enrichment in liquid Vogel-Johnson procedure. The average isolation percentage for other methods ranged from 19.4% to 25.0%. Isolation of S. aureus from milking unit liner or teat skin swabbing solutions was approximately twice as likely after enrichment in Vogel-Johnson liquid media as opposed to other methods of isolation. This indicates that enrichment in Vogel-Johnson liquid media improved recovery of S. aureus from swabbing solutions.  相似文献   

Effect of pre‐milking teat disinfection on clinical mastitis incidence in a dairy herd in Northern Queensland,Australia          下载免费PDF全文

SM Rowe  WP Tranter  RA Laven 《Australian veterinary journal》2018,96(3):69-75

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A comparison of the effect of short-acting and long-acting cloxacillin-based dry-cow therapy on somatic cell counts after calving in cows also given internal teat sealants     

LK Whitfield  RA Laven 《New Zealand veterinary journal》2018,66(1):44-47

AIM: To compare, in cows treated with an internal teat sealant, the effect of short-acting and long-acting cloxacillin-based dry-cow therapy on somatic cell counts (SCC) after calving.

METHODS: Cows from a spring-calving, pasture-based dairy farm in the Manawatu-Whanganui region of New Zealand were randomly allocated to receive either a short-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=291) or a long-acting cloxacillin and ampicillin dry-cow therapy and internal teat sealant (n=288) at the end of lactation. Cows were managed on-farm with routine husbandry procedures through the dry period and following calving. A multivariable logistic regression model was used to determine the association between length of action of dry-cow therapy and the proportion of cows with a SCC >150,000?cells/mL at the first herd test after calving.

RESULTS: Age of cow, mean SCC for the preceding season and interval from calving to the first post-calving herd test were all associated with the proportion of cows with an individual SCC >150,000?cells/mL at the first herd test (p<0.001) Treatment with the short-acting dry-cow therapy was not associated with decreased odds of cows having a SCC >150,000?cells/mL at the first herd test compared with treatment with long-acting dry-cow therapy (OR=0.724; 95% CI=0.40–1.30).

CONCLUSIONS AND CLINICAL RELEVANCE: In this herd, which routinely used internal teat sealants, the use of short-acting cloxacillin-based dry-cow therapy did not result in an increased proportion of cows with elevated SSC post-calving. This was a single farm, single year study but indicates that in this herd, changing from a long-acting to a short-acting antimicrobial may have no impact on the prevalence of subclinical mastitis.  相似文献   

Commercial Seed Lots Exhibit Reduced Seed Dormancy in Comparison to Wild Seed Lots of Echinacea purpurea     

Qu L  Wang X  Chen Y  Scalzo R  Widrlechner MP  Davis JM  Hancock JF 《HortScience : a publication of the American Society for Horticultural Science》2005,40(6):1843-1845

Seed germination patterns were studied in E. purpurea (L.) Moench grouped by seed source, one group of seven lots from commercially cultivated populations and a second group of nine lots regenerated from ex situ conserved wild populations. Germination tests were conducted in a growth chamber in light (40 μmol·m(-2)·s(-1)) or darkness at 25 °C for 20 days after soaking the seeds in water for 10 minutes. Except for two seed lots from wild populations, better germination was observed for commercially cultivated populations in light (90% mean among seed lots, ranging from 82% to 95%) and in darkness (88% mean among seed lots, ranging from 82% to 97%) than for wild populations in light (56% mean among seed lots, ranging from 9% to 92%) or in darkness (37% mean among seed lots, ranging from 4% to 78%). No germination difference was measured between treatments in light and darkness in the commercially cultivated populations, but significant differences were noted for treatments among wild populations. These results suggest that repeated cycles of sowing seeds during cultivation without treatments for dormancy release resulted in reduced seed dormancy in E. purpurea.  相似文献   

Effects of dietary electrolyte balance on the chemistry of blood and urine in lactating sows and sow litter performance     

DeRouchey JM  Hancock JD  Hines RH  Cummings KR  Lee DJ  Maloney CA  Dean DW  Park JS  Cao H 《Journal of animal science》2003,81(12):3067-3074

One hundred fifty-three sows (average parity of 2.2) were used to determine the effects of dietary electrolyte balance (calculated as mEq/kg of diet for Na + K - Cl) on sows and their litters during lactation. The sows were fed corn-soybean meal-based diets (1.0% lysine, 1.0% valine, 0.95% Ca, and 0.80% P; as-fed basis) starting on d 109 of gestation and throughout the 21-d lactation experiment. Dietary electrolyte balance (dEB) was 0, 100, 200, 350, and 500 mEq/kg (as-fed basis), well above and below the dEB of 185 mEq/kg found in a simple corn-soybean meal-based lactation diet. To achieve the desired dEB, diets had the following: 1) 1.8% HCl (6 N) and 1.06% CaCl2, 2) 1.0% CaCl2, 3) 0.04% NaHCO3, 4) 1.29% NaHCO3, and 5) 2.54% NaHCO3 (as-fed basis). Increasing dEB increased blood pH (linear and quadratic effects, P < 0.001), partial pressure of carbon dioxide (linear effect, P < 0.001), HCO3- concentration (linear and quadratic effects, P < 0.001), and blood base excess (linear and quadratic effects, P < 0.001). However, increased dEB resulted in lower blood concentrations of K (linear and quadratic effects, P < 0.04), Cl (linear and quadratic effects, P < 0.001), and ionized Ca (linear and quadratic effects, P < 0.001). Changing dEB did not affect ADFI; water usage, litter weight gain; sow weight change; sow backfat change; percentages of CP, lactose, and fat in the milk; percentage of sows returning to estrus; days to estrus; and number of pigs born alive in the subsequent litter (P = 0.06). However, piglet survivability to d 10 and overall was greatest with the lower dEB treatments (linear effect, P < 0.05). The pH (linear and quadratic effects, P < 0.001) and colony forming units of total bacteria (linear effect, P < 0.03) in the urine increased as dEB of the diet was increased. In conclusion, dEB had pronounced effects on the physiological status of sows and decreasing dEB below that in a simple corn-soybean meal-based diet decreased bacterial counts in the urine and increased piglet survivability. However, milk composition, sow and litter weights at weaning, and subsequent rebreeding performance of the sows were not affected by dEB.  相似文献   

Farm assurance schemes     

Hancock R 《The Veterinary record》2003,153(16):507

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Characterization of,and immune responses of mice to,the purified OmpA-equivalent outer membrane protein of Pasteurella multocida serotype A:3 (Omp28)     

Gatto NT  Dabo SM  Hancock RE  Confer AW 《Veterinary microbiology》2002,87(3):221-235

Pasteurella multocida A:3 is a major cause of bovine pneumonia. A major antigenic heat-modifiable 28kDa outer membrane protein (Omp28) was previously identified. The purpose of this study was to purify and characterize Omp28 immunologically and structurally. Omp28 was extracted from N-lauroylsarcosine-insoluble protein preparations by a combination of detergent fractionation with Zwittergent 3-14 and chromatography. Partial N-terminal amino acid sequence confirmed Omp28 as a member of the OmpA-porin family. However, porin activity could not be demonstrated in a lipid-bilayer assay. Heat modifiability of purified Omp28 was demonstrated, and Omp28 was found in outer membrane fraction of P. multocida. Surface exposure of Omp28 was demonstrated by partial protease digestion of intact bacteria, by binding of anti-Omp28 polyclonal ascites fluid to the bacterial surface, and by partial inhibition of anti-outer membrane antiserum binding by previous incubation of the bacteria with anti-Omp28 serum. CD-1 mice vaccinated with purified Omp28 developed a significant antibody titer (P<0.05) compared to the control treatment group but were not protected from a homologous intraperitoneal bacterial challenge. By contrast, treatment groups vaccinated with P. multocida outer membrane, formalin-killed P. multocida or a commercial vaccine were significantly protected from challenge. In vitro complement-mediated killing of P. multocida was observed in post-vaccination sera of outer membrane, formalin-killed P. multocida, and commercial vaccine-treatment groups, but not with sera from the Omp28-treatment group. In conclusion, although Omp28 is surface exposed and antigenic, it may not be a desirable immunogen for stimulating immunity to P. multocida.  相似文献   

What is your diagnosis? A large, oval, radiolucent defect in the proximolateral portion of the humerus     

Hancock RB  Rashmir-Raven AM  Thomas MW  Marti JM  Fleming SA 《Journal of the American Veterinary Medical Association》2000,217(6):823-824

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Acute cortisol responses of calves to scoop dehorning using local anaesthesia and/or cautery of the wound   总被引：1，自引：0，他引：1  

SP SYLVESTER  DJ MELLOR  KJ STAFFORD  RA BRUCE  RN WARD 《Australian veterinary journal》1998,76(2):118-122

Objective To measure plasma cortisol responses in calves dehorned using a scoop after administration of local anaesthesia and/or cautery of the wounds.
Design A physiological study with controls.
Procedure There were six treatments: control handling with and without local anaesthesia, dehorning, dehorning after local anaesthesia, dehorning followed by wound cautery, and dehorning after local anaesthesia followed by wound cautery. Blood samples were taken before and after dehorning.
Results Dehorning caused an increase in plasma cortisol concentrations, which decreased a little to plateau values and then declined to pretreatment values 3 to 4 h after dehorning. The peak was smaller after local anaesthesia was administered but when its effects wore off, cortisol concentrations increased and thereafter were similar to those in the dehorned animals. The combination of local anaesthesia and cautery resulted in a plasma cortisol response similar to those in control calves with or without local anaesthesia.
Conclusions If plasma cortisol concentrations reflect the distress being experienced by the calves, then local anaesthesia reduces the acute distress for about 3 h after dehorning but not during the subsequent 3 to 4 h. Combining local anaesthetic and cautery prevented the significant increase in plasma cortisol following dehorning and may eliminate the acute distress caused by scoop dehorning.  相似文献   

Successful medical treatment of splenic abscesses in a horse     

CM STEEL  RA LONSDALE  JR BOLTON 《Australian veterinary journal》1998,76(8):541-542

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