Analytical validation of platelet microparticle quantification in cats          下载免费PDF全文

Signe E. Cremer  Jørgen Koch  Nanna Graversen  Anne S. Gravgaard  Rebecca Langhorn  Annemarie T. Kristensen  Jakob L. Willesen  Lise N. Nielsen 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2018,47(3):386-395

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Segment-based coancestry,additive relationship and genetic variance within and between the Norwegian and the Swedish Fjord horse populations     

Hanne Fjerdingby Olsen  Saija Tenhunen  Nils Ivar Dolvik  Dag Inge Våge  Gunnar Klemetsdal 《Acta Agriculturae Scandinavica, Section A - Animal Sciences》2020,69(1-2):118-126

ABSTRACT

The Fjord horse originates from Norway but forms a global population due to several small populations in foreign countries. There exists no information about the additive relationship and the genetic variance between these subpopulations. By collecting blood samples from Norwegian and Swedish Fjord horses, a sample of 311 Norwegian and 102 Swedish horses gave 485,918 SNPs available for analysis. Their inbreeding coefficients were calculated and compared to the pairwise coancestry and the shared genomic segments. The effective population size was almost similar with the two methods in the Norwegian Fjord horse population (63 and 71), but very different in the Swedish population (269 and 1136) and unprecise due to a much smaller number of observations. The study showed that coancestry from shared genomic segments can be used to estimate additive genetic relationship and genetic variation within and between the global populations of the Fjord horse.  相似文献   

Variation in growth and aggression of juvenile brown trout (Salmo trutta) from upstream and downstream reaches of the same river     

E. A. Kristensen  G. P. Closs 《Ecology of Freshwater Fish》2008,17(1):130-135

Abstract –  To investigate differences in behaviour and growth between juvenile brown trout from spatially separated reaches, fish were collected from above and below a weir in Silverstream, New Zealand. Population level differences in mass-specific growth rates and aggression level were examined in tank experiments. Monitoring of mass-specific growth rates found that relative growth rates between fish of different sizes varied between fish from the upstream reach but not between fish from the downstream reach. Large individuals grew faster than smaller individuals from the upstream reach but no effect of relative fish size on growth was found among fish from the downstream population, indicating that social organisation in the two populations differed. When tested in pairs, juvenile brown trout from the downstream population were found to be more aggressive than those from the upstream population. Differences in the allometric relationship between growth rate and fish size and in the levels of aggressiveness appear to be related to the failure to form dominance hierarchies among fish from the downstream reach, a fixed behavioural trait most likely related to prior experience of crowding or different genetic backgrounds.  相似文献   

A two-year comparison on nutritive value and yield of eight lucerne cultivars and one red clover cultivar     

Dannylo O. Sousa  Hanne H. Hansen  Ola Hallin  Luiz G. Nussio  Elisabet Nadeau 《Grass and Forage Science》2020,75(1):76-85

Red clover (Trifolium pratense) and lucerne (Medicago sativa) are among the most common legume species used for ruminant feeding systems. Besides the greater nutritive quality and first-year ley yield compared to lucerne, red clover decreases its yield over the years. However, under unfavourable soil conditions the persistency of lucerne can be compromised, not performing as expected. This study compared yield and chemical composition of eight lucerne cultivars and one tetraploid red clover cultivar, with similar growth capacity as lucerne, replicated over two years. In the second year, four lucerne cultivars and the red clover cultivar were analysed for crude protein (CP) fractions, dry matter and neutral detergent fibre (NDF) digestibility. A randomized block design was used with three field blocks per cultivar for the multiple comparisons within each year. There were differences in DM yield, concentrations of CP and NDF and CP fractions among the lucerne cultivars, but the differences were inconsistent between years and between growth cycles within year. The red clover cultivar had lower CP and NDF concentrations than a majority of the lucerne cultivars, and greater in vitro organic matter digestibility compared to all of the lucerne cultivars. Also, the red clover cultivar had greater rumen undegradable-to-degradable CP ratio but confirmed its lower yield persistency compared to the lucerne cultivars.  相似文献   

Bleeding of Farmed Atlantic Cod: Residual Blood,Color, and Quality Attributes of Pre- and Postrigor Fillets as Affected by Perimortem Stress and Different Bleeding Methods     

Hanne Digre  Ulf Erikson  Ekrem Misimi  Inger Beate Standal  Lorena Gallart-Jornet  Siriporn Riebroy 《Journal Of Aquatic Food Product Technology》2013,22(4):391-411

The main objectives of the study were to (a) assess the effect of perimortem stress on blood drainage, (b) compare the efficiency of gill cutting and direct gutting as bleeding methods, and (c) compare pre- and postrigor filleting strategies for presence of residual blood in Atlantic cod fillets. Anesthetized cod had significantly higher drainage of blood compared to stressed fish. Nevertheless, the visual assessments of residual blood were not affected by stress or bleeding method. Some minor, but significant, differences between pre- and postrigor fillets were found. Stressed fish were initially less light in color than those unstressed, but after ice storage there was no noticeable difference. However, initially, the largest difference in fillet color was due to different bleeding methods. Fillets cut from fish subjected to gill cutting were lighter and less red in color than those bled by direct gutting. After 7 and 21 days of storage, the color differences observed in fresh and salted fillets, respectively, were mainly due to the different rigor status when they were filleted, indicating that fillets cut postrigor were superior to fillets cut prerigor. Prerigor filleting resulted in lower water holding capacity after ice storage in anesthetized, direct gutted cod.  相似文献   

Prüfung von zwei Rattenvertilgungsmitteln     

Dr. R. Hanne 《Journal of pest science》1933,9(4):50-52

Ohne Zusammenfassung  相似文献   

Rumen microbial sequestration of [2-(13)C]acetate in cattle     

Kristensen NB 《Journal of animal science》2001,79(9):2491-2498

To investigate the impact of rumen microbial sequestration of VFA carbon on estimates of acetate availability based on intraruminal infusion of [2-(13)C] acetate, three nonlactating or low-yielding dairy cows were continuously intraruminally infused with [2-(13)C]acetate for 26 h. The 13C content of ruminal VFA, duodenal carbon, and fatty acids (FA) and AA isolated from liquid-associated ruminal microbes and duodenal DM was measured by an isotope ratio mass spectrometer interfaced to an elemental analyzer or a gas-liquid chromatograph. The ruminal gross production of acetate was 38 +/- 4 mol/d and could account for about 38% of the DE intake. Of the intraruminally infused 13C in [2-(13)C]acetate, 7.6 +/- 0.9% was recovered at the duodenum. The 13C content of ruminal propionate, butyrate, and valerate increased (P < 0.05) with intraruminal infusion of [2-(13)C]acetate. It was estimated that about 28% of the 13C intraruminally infused in [2-(13)C]acetate could be accounted for by duodenal 13C flow and absorption of non-acetate VFA. A number of FA isolated from liquid-associated ruminal microbes (C6, C12, C14, anteiso C15, and iso C15) were enriched with 13C (P < 0.05) at a level comparable to the enrichment of ruminal butyrate. Any absorption of these FA from the rumen would further contribute to non-acetate 13C uptake. A maximum of 72% of the ruminal gross production of acetate represented acetate absorption from the rumen in the present study. Consequently, previously used models using intraruminal isotope dilution techniques seem not to be appropriate for measuring acetate availability in ruminants. The number of metabolites exchanging carbon with acetate was found to be so high that assessments of the entire range of inter conversions seem to be practically impossible. Portal absorption studies are discussed as an alternative method of estimating VFA availability to the metabolism in ruminants.  相似文献   

CONTRAST-ENHANCED ULTRASONOGRAPHY IN NORMAL CANINE LIVER. EVALUATION OF IMAGING AND SAFETY PARAMETERS     

Helena T. Nyman  Annemarie T. Kristensen  Mads Kjelgaard-Hansen  Fintan J. McEvoy 《Veterinary radiology & ultrasound》2005,46(3):243-250

Contrast-enhanced ultrasonography, a new imaging modality in veterinary medicine, can provide data on tissue perfusion. The objective of this study was to use the ultrasonographic contrast agent SonoVue to evaluate various transit time indices in the normal canine liver, to examine the effect of anesthesia on these parameters, and to evaluate the safety of this agent in dogs. The liver of 11 healthy dogs was studied by ultrasound during an intravenous bolus injection of SonoVue. Each dog underwent the examination twice, first with and later without the use of anesthesia. A time-intensity curve was generated from a selected region of interest within the liver from each scanning session. Ratios derived from peak enhancement, time to peak enhancement, up-slope and full-width half-maximum (FWHM) of the curve were calculated from the time-intensity curves, and are reported. There were no statistically significant differences (P > 0.05) in peak enhancement, up-slope and FWHM between dogs that were anesthetized and dogs that were not. Time to peak enhancement, however, was significantly shorter when the dogs were anesthetized than when they were nonanesthetized (P < 0.05). There were no biologically significant changes in clinical laboratory findings. This study indicates that contrast-enhanced ultrasound using SonoVue gives reproducible liver perfusion data, and appears to be a safe and well-tolerated agent for use in dogs. When considering normal values, the use of anesthetic drugs has to be considered.  相似文献   

Decreased platelet function in Cavalier King Charles Spaniels with mitral valve regurgitation     

Tarnow I  Kristensen AT  Texel H  Olsen LH  Pedersen HD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2003,17(5):680-686

With aggregometry, increased platelet activity has been reported in Cavalier King Charles Spaniels (CKCS) without mitral regurgitation (MR). In contrast, dogs with MR have been found to have decreased platelet activity. The purpose of this study was to test an easy bedside test of platelet function (the Platelet Function Analyzer [PFA-100]) to see if it could detect an increase in platelet activity in CKCS without MR and a decrease in platelet activity in CKCS with MR. This study included 101 clinically healthy dogs > 1 year of age: 15 control dogs of different breeds and 86 CKCS. None of the dogs received medication or had a history of bleeding. The PFA-100 evaluates platelet function in anticoagulated whole blood under high shear stress. Results are given as closure times (CT): the time it takes before a platelet plug occludes a hole in a membrane coated by agonists. The CT with collagen and adenosine-diphosphate as agonists was similar in control dogs (median 62 seconds; interquartile interval 55-66 seconds) and CKCS with no or minimal MR (55; 52-64 seconds). The CT was higher in CKCS with mild MR (regurgitant jet occupying 15-50% of the left atrial area) (75; 60-84 seconds; P = .0007) and in CKCS with moderate to severe MR (jet > 50%) (87: 66-102 seconds; P < .0001). CKCS with mild, moderate, and severe, clinically inapparent MR have decreased platelet function. The previous finding of increased platelet reactivity in nonthrombocytopenic CKCS without MR could not be reproduced with the PFA-100 device.  相似文献   

Development of a rapid in vitro protein refolding assay which discriminates between peptide-bound and peptide-free forms of recombinant porcine major histocompatibility class I complex (SLA-I)   总被引：3，自引：0，他引：3  

Oleksiewicz MB  Kristensen B  Ladekjaer-Mikkelsen AS  Nielsen J 《Veterinary immunology and immunopathology》2002,86(1-2):55-77

The extracellular domains of swine leukocyte antigen class I (SLA-I, major histocompatibility complex protein class I) were cloned and sequenced for two haplotypes (H4 and H7) which do not share any alleles based on serological typing, and which are the most important in Danish farmed pigs. The extracellular domain of SLA-I was connected to porcine beta2 microglobulin by glycine-rich linkers. The engineered single-chain proteins, consisting of fused SLA-I and beta2 microglobulin, were overexpressed as inclusion bodies in Escherichia coli. Also, variants were made of the single-chain proteins, by linking them through glycine-rich linkers to peptides representing T-cell epitopes from classical swine fever virus (CSFV) and foot-and-mouth disease virus (FMDV). An in vitro refold assay was developed, using a monoclonal anti-SLA antibody (PT85A) to gauge refolding. The single best-defined, SLA-I restricted porcine CD8(+) T-cell epitope currently known is a 9-residue peptide from the polyprotein of CSFV (J. Gen. Virol. 76 (1995) 3039). Based on results with the CSFV epitope and two porcine haplotypes (H4 and H7), the in vitro refold assay appeared able to discriminate between peptide-free and peptide-occupied forms of SLA-I. It remains to be seen whether the rapid and technically very simple in vitro refold assay described here will prove generally applicable for the screening of virus-derived peptides for SLA-I binding.  相似文献