Detection of Pasteurella multocida isolates from local pigs of India by polymerase chain reaction and their antibiogram     

Rajkhowa S  Shakuntala I  Pegu SR  Das RK  Das A 《Tropical animal health and production》2012,44(7):1497-1503

Pasteurella multocida has been recognized as an important veterinary pathogen for over a century. Conventional methods for diagnosis of pasteurellosis rely on the detection of the organism by microscopy and its isolation and identification. However, as far as pasteurellosis is concerned, it is not just sufficient to know the identity of the organism. To constitute effective control measures, it is important to know the serotype of the organism. A study was undertaken to characterize the Pasteurella isolates from local pigs in India with clinical respiratory disease by determination of their capsule types and presence or absence of toxin gene. Pasteurella could be isolated from 66.70% of pigs with clinical respiratory disease. All the isolates were confirmed through biochemical characterization and P. multocida-specific polymerase chain reaction. It has also been observed that all the isolates belonged to capsular type D. All the isolates were sensitive to chloramphenicol, chlortetracycline, doxycycline, and enrofloxacin, while the rest of the antibiotics were less effective. It has also been observed that all isolates were resistant to cephalexin, penicillin G, and sulphadiazine. The study revealed the detection of P. multocida serotype D from clinical respiratory diseases of local pigs of India, which could be one of the important respiratory tract pathogens responsible for mortality of local pigs in India.  相似文献   

Impact of boron fertilization on boron fractions at different crop growth stages in cauliflower_cowpea_okra sequence in an inceptisols of North East India     

Ingudam Bhupenchandra  Anjali Basumatary  Samiron Dutta  Laishram Kanta Singh  Namita Datta 《Journal of plant nutrition》2020,43(8):1175-1188

Abstract

A field experiment was conducted at Horticultural Experimental Farm, Assam Agricultural University, Jorhat during the year 2015–17 to assess the impact of boron fertilization on dynamics of boron fractions in soil and crop yields in cauliflower–cowpea–okra cropping sequence. Five levels of boron (0, 0.5, 1.0, 1.5 and 2.0?kg ha^?1) along with the recommended dose of NPK fertilizer were applied in soil in cauliflower once in each 2 years (2015–17) of experimentation to assess the direct effect of boron fertilization, while cowpea and okra were grown as a suceeding test crop to study the residual effects of boron fertilization in the cropping sequence. Among the different levels of boron, the highest content of all the boron fractions in soil was recorded at rate of 2?kg B ha^?1 at different crop growth stages for all the three crops. Progressive decrease in content of all the boron fractions with crop ageing was noticed. The content of different boron fractions in soil follows the order readily soluble boron (RSB)<oxide bound boron (OXB)<organic bound boron (ORBB)<specifically adsorbed boron (SAB) <residual boron, respectively. The boron level of 2?kg B ha^?1 was found to prominent in increasing the yield in all the crops. In respect of contributions of different boron fractions to yield of crops, oxide bound boron(2.10) was found to be the highest contributor of cauliflower yield, while the residual boron (1.10), contributed the highest to cowpea yield and organic bound boron fraction (1.24) was found to be most prominent contributor of boron in leveraging okra yield.  相似文献