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A greenhouse experiment was carried out to investigate the effects of different growing media on some properties of tomato (Lycopersicom esculentum L. cv. Falcato). Four growing media including wheat residue, composted wheat residue, coco peat + perlite, and coco peat + soil were applied in pot experiment with completely randomized design with three replications. The results showed that fruit yield, chlorophyll, and vitamin C contents of tomatoes grown in the composted wheat residue and coco peat + perlite treatments were significantly greater than the other growth media. Fresh weight of root and inflorescence were higher in the tomatoes cultivated in the composted wheat residue pots than the other media. Higher internode length and nutrient concentrations in leaf and fruit at the coco peat + perlite treatment were obtained as compared to the other growth media treatments. According to the results, it can be concluded that the coco peat + perlite can be substituted for by composted wheat residue for the cultivation of tomato. 相似文献
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Magnetic Resonance Imaging of the Normal Canine Larynx 总被引：1，自引：0，他引：1
J. M. Vázquez A. Arencibia F. Gil J. A. Ramírez N. González C. D. Sosa J. R. Jaber 《Anatomia, histologia, embryologia》1998,27(4):263-270
The purpose of this study was to define the normal anatomic structures in the canine larynx with magnetic resonance images. TI-weighted images were taken in the sagittal and transverse planes. The MR images were obtained comparing MR images to dissection planes. Magnetic resonance imaging provides excellent anatomic detail of laryngeal structures. Therefore, it is of value for diagnostic imaging of some respiratory diseases in the dog. 相似文献
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Barbour EK Saade MF Sleiman FT Hamadeh SK Mouneimne Y Kassaifi Z Kayali G Harakeh S Jaber LS Shaib HA 《Tropical animal health and production》2012,44(7):1513-1519
The purpose of this research is to optimize quantitatively the amplification of specific sperm genes in reference genomically characterized Saanen goat and to evaluate the standardized protocols applicability on sperms of uncharacterized genome of rural goats reared under subtropical environment for inclusion in future selection programs. The optimization of the protocols in Saanen sperms included three production genes (growth hormone (GH) exons 2, 3, and 4, αS1-casein (CSN1S1), and α-lactalbumin) and two health genes (MHC class II DRB and prion (PrP)). The optimization was based on varying the primers concentrations and the inclusion of a PCR cosolvent (Triton X). The impact of the studied variables on statistically significant increase in the yield of amplicons was noticed in four out of five (80%) optimized protocols, namely in those related to GH, CSN1S1, α-lactalbumin, and PrP genes (P??0.05). The applicability of the optimized protocols of Saanen sperm genes on amplification of uncharacterized rural goat sperms revealed a 100% success in tested individuals for amplification of GH, CSN1S1, α-lactalbumin, and MHC class II DRB genes and a 75% success for the PrP gene. The significant success in applicability of the Saanen quantitatively optimized protocols to other uncharacterized genome of rural goats allows for their inclusion in future selection, targeting the sustainability of this farming system in a subtropical environment and the improvement of the farmers livelihood. 相似文献
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Hedman C Lyahyai J Filali H Marín B Serrano C Monleón E Moreno B Zaragoza P Badiola JJ Martín-Burriel I Bolea R 《Veterinary microbiology》2012,158(1-2):23-32
Bluetongue viruses (BTVs) could invade N-W Europe similar to BTV serotype 8 (BTV8/net06), since the source and route of introduction of this virus has not been solved. Therefore, the Dutch survey for Bluetongue by PCR testing was extended by further analysis of PCR positives to identify the involved BTV. In late August 2008, BTV was reported with 12 nucleotide differences in the S10 amplicon (S10 genotyping). This virus was identified as serotype 6, here named BTV6/net08. Promptly, serotype specific real-time PCR tests were developed for serotypes 1, 6, and 8 (S2 genotyping). Agreement was found between results by S10- and S2 genotyping. Further, BTV1 was identified by both S10- and S2 genotyping in one imported animal. After initial discovery of BTV6 in the Netherlands, animals from 18 holdings tested PCR positive for BTV6/net08 in 2008. Remarkably only one or two PCR positive animals per holding were found. Serum neutralization tests did not result in the discovery of more BTV6 infected animals. Retrospective studies indicated no evidence for infections by BTV6/net08 prior to the first discovery. Experimental infections with BTV6/net08 did not cause clinical disease in sheep, calves and cattle, except for a very short fever in some animals. This clearly showed that the vaccine-related BTV6/net08 is not virulent. BTV6/net08 was not found by passive and active surveys in the years after its discovery. Apparently, BTV6/net08 was not efficiently transmitted by endemic species of Culicoides in N-W Europe, and disappeared without the need of any control measure. 相似文献
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Sierra E Fernández A Espinosa de Los Monteros A Jaber JR Andrada M Herráez P 《Veterinary journal (London, England : 1997)》2012,193(1):152-156
Skeletal muscle samples were examined post-mortem in 148 cetaceans over a 12-year period. Histological analysis included haematoxylin and eosin (HE) and periodic acid-Schiff (PAS) staining with and without diastase digestion. In addition, histological muscle sections were immunostained for ubiquitin and fast and slow heavy-chain myosin isoforms. PAS-positive, diastase-resistant inclusions were detected in 26 animals from 11 different species. Older cetaceans were preferentially affected. These intrafibre inclusions varied from large aggregates to multiple coarse granules and were typically associated with type II fibres. All diastase-resistant inclusions were positive for ubiquitin. These features resembled those inclusions described as complex polysaccharide in horses. Based on these histological findings and the ubiquitin staining pattern, a morphological diagnosis of complex polysaccharide storage myopathy is proposed. 相似文献
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Alireza Hadipour Majid Azizi Hasanali Naghdi-Badi Jaber Panahandeh Abbas Delazar Hosein Aroei 《The Journal of Horticultural Science and Biotechnology》2016,91(1):55-62
A karyotype study was performed on 15 populations of Eremostachys laciniata Bunge from Iran in order to quantify the extent of cytological variation for use in breeding programmes. Ten mitotic root tip cells at the metaphase stage were prepared from each population by the squash method. The chromosomes of suitable mitotic cells were counted and various parameters, including long and short arm lengths, chromosome lengths, total length of the haploid chromosome complement, arm ratios, and the centromeric index, were measured. All 15 populations were diploid with 2n = 2x = 22 and were differentiated by their karyotypic parameters. All 15 E. laciniata populations occupied Class 1A of Stebbins’ karyotype classification, indicating the presence of a primitive symmetrical karyotype. The size of the mitotic chromosomes was medium, and mean chromosome lengths ranged from 3.84 to 4.77 μm. The shortest chromosome lengths were observed in the Ajabshir population and the longest in the Heydarabad population. The Heydarabad population also had the longest total chromosome length and the Ajabshir population had the shortest total chromosome length. The centromeric indices of the chromosome complements varied from 39.3% to 43.7%. Metacentric chromosomes were the most common in all populations, whereas submetacentric chromosomes were rare. The A1 index varied from 0.215 (Areshtanab) to 0.337 (Til). The A2 index ranged from 0.116 (Til) to 0.143 (Kaleybar), and the karyotype asymmetry index varied between 0.051 and 0.097. The results of a principal component analysis (PCA) showed that the main elements of the first three main principal components accounted for 97% of the total variance. Cluster analysis by Ward’s method indicated that all 15 populations could be classified into three clusters. Finally, the results of the karyotypic parameters showed that the Til, Heydarabad, and Marand populations had higher levels of karyotypic heterogeneity and may be useful when developing breeding programmes. 相似文献
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K S Ghenghesh S S Abeid M M Jaber S A Ben-Taher 《Comparative immunology, microbiology and infectious diseases》1999,22(3):175-179
Rectal swabs from 120 domestic dogs and 15 domestic cats were examined for Aeromonas species using alkaline peptone water (pH 8.6) as the enrichment medium and blood agar containing 15 mg/l ampicillin as the plating medium. Aeromonads were isolated from 13 (10.8%) dogs and from 1 (6.7%) cat. Of the 14 aeromonads isolated in the present study only 9 were available for speciation and testing in the haemolysin assay. Of these 5 were A. sobria (including one from a cat), 2 were A. hydrophila and 2 were A. caviae. Six were positive in the haemolysin assay; 4 A. sobria (one from a cat) and 2 A. hydrophila. The presence of haemolysin producing-Aeromonas species in the faeces of domestic dogs and cats may pose a public health problem for humans who come into contact with such animals. 相似文献
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Sharififar F Mirtajadini M Azampour MJ Zamani E 《Pakistan journal of biological sciences: PJBS》2012,15(1):49-53
One of the most common strategies in the treatment of cognitive disorders is enhancing the acetylcholine level in the brain through the inhibition of acetylcholinesterase. Despite the effectiveness of current modern drugs, more attention has been paid for finding new anticholinesterase agents from medicinal plants. Zatraia multiflora Boiss. is an endemic plant to Iran which has different uses in traditional medicine as anti-inflammatory, antimicrobial, anti spasmodic. We intended to evaluate the in vitro anticholinesterase and free radical scavenging activity of the essential oil and methanolic extract of Z. multiflora. The essential oil and methanolic extract of the plant were evaluated for anticholinesterase activity using modified Ellman method. The free radical scavenging effect of the samples were studied by using of the diphenylpicrylhydrazyl (DPPH). IC50 and the percent of inhibition of acetylcholinesterase was calculated from regression equation. The results showed that both the essential oil and methanolic extract of the plant exhibited high anticholinesterase activity (95.3 +/- 3.4 and 87.9 +/- 2.2% inhibition, respectively) which was similar to eserine (96.2 +/- 1.7% inhibition). The IC50 value of essential oil was determined as 0.97 +/- 0.12 microg mL(-1) in comparison to eserine (0.13 +/- 0.02 microg mL(-1)). The results of antioxidant assay showed that both the essential oil and methanolic extract potentially inhibit DPPH free radical (94.8 +/- 2.4 and 93.2 +/- 1.7% inhibition, respectively). The essential oil and methanolic extract of Z. multiflora have beneficial effect in health promotion and this plant would be good candidate for further studies. 相似文献