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排序方式: 共有59条查询结果,搜索用时 843 毫秒
1.
A study was conducted in a sweet pepper-maize-rice cropping system in six farmers fields in Batac, Ilocos Norte, the Philippines, to determine the optimum P fertilizer rate for sweet pepper that will benefit the succeeding crops, maximize system-level productivity and profitability, and reduce the excessive accumulation of P in the soil. Single super phosphate was applied to sweet pepper at rates of 0, 28, 56, 84, 112, and 140 kg P ha–1 and the succeeding crops were grown without P fertilization. Maize residue was incorporated into the soil at puddling of soil for rice. Phosphorus fertilization at 56 kg P ha–1 and above had a residual effect on maize and rice. A reduction in the P applied to sweet pepper from 140 to 84 kg P ha–1 reduced extractable P in the soil at rice harvest from 52 to 29 kg P ha–1. Phosphorus applied at 111 kg P ha–1 to sweet pepper was optimum for maximum productivity and economic returns of the sweet pepper-maize-rice cropping system. This rate of P also significantly reduced P accumulation in the soil, thereby reducing the chances of negative effects on soil nutrient balance/availability. The results suggested the need for a cropping systems approach to conserve and effectively use native and fertilizer P in the sweet pepper-rice cropping system. 相似文献
2.
Class II aminoacyl transfer RNA synthetases: crystal structure of yeast aspartyl-tRNA synthetase complexed with tRNA(Asp) 总被引:65,自引:0,他引:65
M Ruff S Krishnaswamy M Boeglin A Poterszman A Mitschler A Podjarny B Rees J C Thierry D Moras 《Science (New York, N.Y.)》1991,252(5013):1682-1689
The crystal structure of the binary complex tRNA(Asp)-aspartyl tRNA synthetase from yeast was solved with the use of multiple isomorphous replacement to 3 angstrom resolution. The dimeric synthetase, a member of class II aminoacyl tRNA synthetases (aaRS's) exhibits the characteristic signature motifs conserved in eight aaRS's. These three sequence motifs are contained in the catalytic site domain, built around an antiparallel beta sheet, and flanked by three alpha helices that form the pocket in which adenosine triphosphate (ATP) and the CCA end of tRNA bind. The tRNA(Asp) molecule approaches the synthetase from the variable loop side. The two major contact areas are with the acceptor end and the anticodon stem and loop. In both sites the protein interacts with the tRNA from the major groove side. The correlation between aaRS class II and the initial site of aminoacylation at 3'-OH can be explained by the structure. The molecular association leads to the following features: (i) the backbone of the GCCA single-stranded portion of the acceptor end exhibits a regular helical conformation; (ii) the loop between residues 320 and 342 in motif 2 interacts with the acceptor stem in the major groove and is in contact with the discriminator base G and the first base pair UA; and (iii) the anticodon loop undergoes a large conformational change in order to bind the protein. The conformation of the tRNA molecule in the complex is dictated more by the interaction with the protein than by its own sequence. 相似文献
3.
Arundhati Das Jagdish Krishnaswamy Kamaljit S. Bawa M.C. Kiran N. Samba Kumar 《Biological conservation》2006,133(1):16-31
Areas of high conservation value were identified in the Western Ghats using a systematic conservation planning approach. Surrogates were chosen and assessed for effectiveness on the basis of spatial congruence using Pearson’s correlations and Mantel’s tests. The surrogates were, threatened and endemic plant and vertebrate species, unfragmented forest areas, dry forests, sub-regionally rare vegetation types, and a remotely sensed surrogate for unique evergreen ecosystems. At the scale of this analysis, amphibian richness was most highly correlated with overall threatened and endemic species richness, whereas mammals, especially wide-ranging species, were better at capturing overall animal and habitat diversity. There was a significant relationship between a remote sensing based habitat surrogate and endemic tree diversity and composition. None of the taxa or habitats served as a complete surrogate for the others. Sites were prioritised on the basis of their irreplaceability value using all five surrogates. Two alternative reserve networks are presented, one with minimal representation of surrogates, and the second with 3 occurrences of each species and 25% of each habitat type. These networks cover 8% and 29% of the region respectively. Seventy percent of the completely irreplaceable sites are outside the current protected area network. While the existing protected area network meets the minimal representation target for 88% of the species chosen in this study and all of the habitat surrogates, it is not representative with regard to amphibians, endemic tree species and small mammals. Much of the prioritised unprotected area is under reserve forests and can thus be incorporated into a wider network of conservation areas. 相似文献
4.
The direct immunoperoxidase test has been used to detect rinderpest virus antigens in infected bovine kidney cell cultures to study the multiplication of the virus. Infected bovine kidney coverslip cultures were sequentially tested with peroxidase-labelled, anti-rinderpest globulins at 3, 6, 24, 48, 72, 96, 120 and 144 h post-infection. The progressive virus-specific cytopathic changes compared well with the increase in the number of cells showing the presence of viral antigens when tested by the direct immunoperoxidase test. The specificity of the reaction was confirmed by using negative and antiserum-blocked BK cell cultures on coverslips. 相似文献
5.
Patel J 《The Canadian veterinary journal. La revue veterinaire canadienne》2004,45(9):720; author reply 721-721
6.
India is the world’s tenth most forested nation with 76.87 M ha of forest and tree cover occupying 23.4% of its geographical
area. Forests—with their intrinsic of carbon sequestration and storage values—are in the front line of India’s climate change
mitigation strategies. This paper provides estimates of sequestered carbon in India’s forest and tree cover for the years
1995 and 2005 as per the IPCC good practice guidelines method. It is based on the primary data for the soil carbon pool through
collecting soil samples by laying out quadrats across the country and secondary data for the growing stock of all forest and
tree cover in the country. The estimates are compared with current and future projected emissions. It is found that conservation
policies have resulted in increase of the country’s forest carbon stocks from 6244.8 to 6621.6 Mt with an annual increment
of 37.7 Mt of the carbon from 1995 to 2005. Annual CO2 removal by the forests is enough to neutralise 9.3% of the country’s 2000 level emissions. Continued removals by the forest
and tree cover would offset 6.5 and 4.9% of India’s projected annual emissions in 2010 and 2020 respectively. Economically,
the annual value of this forest carbon in the international market is about US $188 million. The result is of use in the REDD
and REDD+ context for India. 相似文献
7.
8.
Pankaj Kumar Mishra Shekhar Chandra Bisht Krishnan Jeevanandan Sanjay Kumar Jaideep Kumar Bisht Jagdish Chandra Bhatt 《Archives of Agronomy and Soil Science》2013,59(6):799-815
Plant growth-promoting bacteria (PGPB) Pseudomonas lurida-NPRp15 and Pseudomonas putida-PGRs4 possessing multiple plant growth-promoting traits were isolated from rhizoplane of pea and rhizosphere of garlic, respectively. The effects of individuals and combinations of Pseudomonas spp. with effective root nodulating symbiotic nitrogen fixing Rhizobium leguminosarum-FB1 on plant growth, nutrient uptake and yield of the rajmash plant were studied under greenhouse conditions. Bacterial inoculation resulted in significantly higher values for plant dry biomass, N, P, K, Zn and Fe contents as compared to the uninoculated control. Furthermore, dual inoculation of P. lurida-NPRp15 with R. leguminosarum-FB1 significantly increased root and shoot dry weight, nodulation, nutrient uptake, pod yield, and nutrient content of pods of rajmash VL63 compared to controls, single and triple inoculation. The results of the study indicate the potential of harnessing the benefit of plant growth-promoting and nitrogen-fixing microorganisms to improve the growth and yield of rajmash. 相似文献
9.
Naseer A. Baba Manjit Panigrahi Ankita D. Verma Abdul Sadam Sourabh Sulabh Supriya Chhotaray Subhashree Parida Narayanan Krishnaswamy Bharat Bhushan 《Reproduction in domestic animals》2019,54(1):100-107
Progesterone (P4) plays a key role in the establishment and maintenance of pregnancy in most mammals. Unravelling the expression of progesterone‐regulated genes can expand the understanding of the embryonic mortality. Accordingly, we studied the relative mRNA expression of the P4‐regulated genes in the buffalo. Uteri were collected from the abattoir and categorized into nonpregnant late luteal phase, stage I (28–38th days of gestation) and stage II (48–56th days of gestation) of pregnancy (n = 6/group). After extraction of total RNA from the endometrial tissues, we carried out qRT‐PCR for determining the relative mRNA expression of the P4‐regulated genes using nonpregnant late luteal phase as calibrator group. The expression of LGALS3BP (essential for maternal recognition of pregnancy) gene was found to be significantly upregulated (p < 0.05), while MUC1 (important for embryo attachment) gene was downregulated in stage I and II of pregnancy. We observed no significant change in the expression of LGALS1, LGALS9 and CTSL genes. The SLC5A11 and SLC2A1 genes (involved in the transport of glucose to endometrium) in early pregnancy were upregulated in the pregnancy stage I (p < 0.05) relative to nonpregnant late luteal phase. The CST3 gene was significantly upregulated in pregnancy stage II (p < 0.01). These results provide molecular insights into the specific pathways involved in foeto‐maternal communication during early pregnancy in buffaloes. 相似文献
10.
The economic feasibility of energy farming based on Populus × euramericana (clone I45/51) in eastern Ontario has been evaluated for two potential energy end-uses: direct combustion and methanol production. Optimal management regime and the time when energy farming should begin to be economically most desirable have been determined. Three-year rotations with 16–35 thousand stumps/ha planted and coppiced for 5–6 rotations could be an attractive enterprise in about 15 years. Farming for methanol production could be feasible only about 35 years from now. 相似文献