首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   17211篇
  免费   94篇
林业   3661篇
农学   1351篇
基础科学   142篇
  2893篇
综合类   732篇
农作物   2181篇
水产渔业   1886篇
畜牧兽医   1370篇
园艺   1133篇
植物保护   1956篇
  2023年   6篇
  2022年   16篇
  2021年   21篇
  2020年   32篇
  2019年   62篇
  2018年   2774篇
  2017年   2744篇
  2016年   1229篇
  2015年   100篇
  2014年   56篇
  2013年   80篇
  2012年   830篇
  2011年   2172篇
  2010年   2125篇
  2009年   1273篇
  2008年   1345篇
  2007年   1600篇
  2006年   55篇
  2005年   127篇
  2004年   117篇
  2003年   173篇
  2002年   76篇
  2001年   22篇
  2000年   51篇
  1999年   11篇
  1998年   9篇
  1997年   9篇
  1996年   8篇
  1995年   6篇
  1994年   7篇
  1993年   25篇
  1992年   13篇
  1991年   8篇
  1990年   7篇
  1989年   7篇
  1988年   19篇
  1987年   6篇
  1985年   4篇
  1984年   5篇
  1983年   6篇
  1982年   4篇
  1979年   11篇
  1978年   6篇
  1977年   8篇
  1976年   6篇
  1975年   4篇
  1971年   4篇
  1968年   4篇
  1967年   6篇
  1966年   3篇
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
1.
2.
The microbial community structure and function under forest in tropical peatlands are poorly understood. In this study, we investigated the microbial community structure and diversity in natural peat swamp forest soil, disturbed peat soil and mineral soil in Central Kalimantan, Indonesia, using 454 pyrosequencing. The results showed that the natural peat soil had the greatest fungal species richness (Chao1), which was significantly (< .05) larger than that in the other two soils. Community structure of both fungi and bacteria in natural peat soil differed significantly from that in the disturbed peat soil (= .039 and = .045, respectively). Ascomycota (40.5%) was the most abundant phylum across the three soils followed by Basidiomycota (18.8%), Zygomycota (<0.1%) and Glomeromycota (<0.1%). The linear discriminant analysis with effect size (LEfSe) showed that Ascomycota (< .05) and genus Gliocephalotrichum (< .05) dominated in natural peat soil. Functionally, pathotrophs were more abundant in disturbed peat soil (< .05). Proteobacteria (43.8%) were the most abundant phylum followed by Acidobacteria (32.6%), Actinobacteria (9.8%), Planctomycetes (1.7%). Methylocystis, Telmatospirillum, Syntrophobacter, Sorangium and Opitutus were the more abundant genera in disturbed peat soil, whereas Nevskia and Schlesneria were more abundant in mineral soil and natural peat soil, respectively. The natural peat forest soil supported a more diverse microbiology; however, the land use of such a soil can change its microbial community structure. The results provide evidence that the disturbance of tropical peat land could lead to the introduction and spread of a large number of fungal diseases  相似文献   
3.
4.
Provision of feed supplies for the rapidly growing livestock population is essential in West Asia. Shortages can be alleviated by growing forage crops in the existing fallow lands.
Narbon vetch (Vicia narbonensis L.) is a leguminous species with high yield potential, drought tolerance and cold resistance. It could be used for grain and straw production as source for animal feed in dry areas. It is good source of protein with seeds contain 28 % protein yielding 364 kg per hectare protein, whilst straw contains 9 % protein and yields a similar amounts of digestible protein per hectare.
Nine promising lines of Narbon vetch developed at ICARDA were tested at two sites over four years under rainfed conditions. Since climatic conditions were considered to be of considerable importance, each site in each year was treated as a separate environment to give eight environments with annual rainfall varied from 195 to 504 mm.
Narbon vetch possessed high seedling vigour with rapid winter growth and negligible cold damage. Grain yield varied form 0.47 to 1.90 t/ha, with a harvest index varying from 30 to 40 %. The data indicated that below 300 mm rainfall the grain yield varied from 0.47 t/ha when rain fall was 195 mm to 1.4 t/ha when rainfall was 245 mm. Most of the lines had wide adaptation to dry areas in terms of both grain yield and stability. Climate, except early spring rains had little effect on biological and grain yields.  相似文献   
5.
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   
6.
1. Under summer conditions in north Iraq, 2 000 chicks were reared for 56 d in two broiler houses. One house was provided with two air‐coolers. The birds were housed at densities of eight to 16 birds/m2.

2. Differences in weight gain between birds in cooled and uncooled houses ranged from 101.5 g at eight birds/m2 to 385 g at 16 birds/m2. Food consumption increased gradually with increasing bird density. Food conversion efficiency, meat yield and carcass conformation were much better in the cooled house than under conventional conditions.

3. The financial return/m2 ranged from 2.30 to 4.51 ID1 in the cooled house, compared with 2.01 to 2.77 ID in the uncooled house at different floor densities.  相似文献   

7.
1. Chemical analysis of a sample of ground mango seed kernels (MSK) revealed the following percentage composition: dry matter, 91.55; ash, 1.97; protein, 6.74; ether extract, 12.53; fibre, 1.30; nitrogen‐free extract, 77.46.

2. The ME content of MSK was 1.82 kcal/g.

3. The percentage retention of the crude fat content of MSK was only 3.38%.

4. Substituting MSK for corn at 10 or 20% led to reduced growth rate and poorer food consumption and a reduced efficiency of food utilisation in the period 0 to 4 weeks.  相似文献   

8.
L11A-Fukushima (L11A-F) derived from attenuated isolate LuA of Tomato mosaic virus (ToMV) has the highest ability to cross protect against virulent ToMV among LuA and its derivatives and is stably inherited. Growth, yield, fruit quality and symptom attenuation of inoculated tomato plants did not differ significantly between L11A-F and L11A. The infectivity of progeny viruses in tomato infected with LuA-F was less than 4% of that with virulent ToMV. From these results, L11A-F appears to possess the properties necessary for practical use. To manage L11A-F strictly, a PCR-based assay to detect trace contamination of virulent ToMV in L11A-F preparations was established. Received 10 June 2002/ Accepted in revised form 30 October 2002  相似文献   
9.
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV originating from latently infected stock plants in chrysanthemum production fields. Received 27 July 2001/ Accepted in revised form 27 November 2001  相似文献   
10.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号