Conservative management of an atlantoaxial subluxation using thermoplastic material          下载免费PDF全文

E. L. Hall  J. Guevar  K. L. Holmes  R. Gutierrez‐Quintana 《The Journal of small animal practice》2016,57(7):386-386

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Urinary retention in two male dromedaries due to silica uroliths     

Gutierrez C  Padrón M  Bañares A  Palacios MP 《Zentralblatt für Veterin?rmedizin. Reihe A》1999,46(9):523-526

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The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques     

Gutierrez M  Forster FI  McConnell SA  Cassidy JP  Pollock JM  Bryson DG 《Veterinary immunology and immunopathology》1999,71(3-4):321-334

In order to develop procedures to label the main bovine leucocyte populations in paraffin embedded sections, the immunoreactivity of 25 monoclonal antibodies (mAbs) to different leucocyte antigens was assessed with formal dichromate (FD5) and 10% formalin fixation, a battery of antigen retrieval (AR) methods, and the biotin-tyramide amplification system. All the leucocyte populations investigated (CD2+, CD4+, CD8+, WC1+ T lymphocytes, B cells and macrophages) were strongly and specifically detectable under an appropriate combination of mAb, AR method and signal amplification system. CD4 and CD8 required the most stringent conditions and could only be demonstrated in FD5 fixed sections. For detection of CD2, WC1+ T lymphocytes, B cells and macrophages, all the mAbs produced immunoreactivity in FD5 or formalin fixed tissues. The need to check a range of different AR methods is stressed, as the method of choice varied for each individual mAb. The incorporation of the signal amplification system was necessary to observe a strong signal and the complete distribution of CD4, CD8 and B cells. Fixation by FD5 proved to be better than formalin for the preservation of surface antigens but it was inferior for the detection of markers which were found to show cytoplasmic immunoreactivity, such as the macrophage marker MAC387 or the B cell markers BAQ155 or IL-A59.  相似文献   

Vegetative valvular endocarditis in a dromedary camel     

Gutierrez C  Aguirre M  Morales M  Doreste F  Corbera JA 《The Veterinary record》2002,151(10):300-301

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Silica Urolithiasis in the Dromedary Camel in a Subtropical Climate     

Gutierrez C  Corbera JA  Doreste F  Padrón TR  Morales M 《Veterinary research communications》2002,26(6):437-442

In 1998, two cases of silica urolithiasis occurred in castrated male dromedaries on an intensive camel farm in the Canary Islands. The immediate attributable cause was the ingestion of large amounts of silica in the feed, estimated as 84.44 g/day. An associated cause was the low level of salt in the diet. Daily ingestion of salt from feed and water was estimated to be 21.8 g (8.6 g of sodium). Seventy-six castrated males from the same farm were divided into four groups: group A received 30 g of salt daily; group B received 40 g; group C received 60 g; and group D received no added salt in the diet (control). The animals were maintained on these dietary regimes for 2 years. No animals from groups A, B or C suffered overt urinary retention. One animal from group D had an obstructive urinary retention 10 months after the study commenced. Thus, 52 g of salt daily appears to be sufficient to prevent urinary retention in dromedaries raised in a subtropical climate.  相似文献   

Multiple malformations in a newborn goat          下载免费PDF全文

Gutierrez C  Rodriguez JL  Castellano E  Palomino E  Corbera JA  Montoya JA 《The Canadian veterinary journal. La revue veterinaire canadienne》2000,41(7):568-569

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Severe urolithiasis due to trimagnesium orthophosphate calculi in a goat     

Gutierrez C  Escolar E  Juste MC  Palacios MP  Corbera JA 《The Veterinary record》2000,146(18):534

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Camel trypanosomosis in the Canary Islands: assessment of seroprevalence and infection rates using the card agglutination test (CATT/T. evansi) and parasite detection tests     

Gutierrez C  Juste MC  Corbera JA  Magnus E  Verloo D  Montoya JA 《Veterinary parasitology》2000,90(1-2):155-159

Trypanosomosis due to Trypanosoma evansi (surra) is a major enzootic disease of the dromedary camel. Thus, the purpose of the present study was to assess seroprevalence and infection rates in the Canary Islands using antibody(-card agglutination test-CATT/T. evansi) and parasite detection tests (micro-Haematocrit Centrifugation technique, Giemsa stained blood smears, microscopic examination of lymph node aspirates and mouse inoculation). PCV was also determined. 745 dromedary camels (483 females and 262 males) were examined. Trypanosomes were detected in seven animals. 36 animals yielded CATT positive results while 709 animals were negative. All parasitologically positive animals were also CATT positive. Results showed a good correlation between CATT positive and low PCV and a higher seroprevalence in older animals. Trypanocidal drugs have not been registered in Spain and, consequently, if vigilance is not exercised the prevalence could be increased in the future.  相似文献   

Early growth, biomass allocation and physiology in three Eucalyptus nitens populations under different water regimes     

Gutierrez  L.; Casares  A.; Sanchez-Tames  R.; Majada  J. 《Forestry》2002,75(2):139-148

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GROSS AND HISTOPATHOLOGIC CORRELATION OF LOW‐FIELD MAGNETIC RESONANCE IMAGING FINDINGS IN THE STIFLE OF ASYMPTOMATIC HORSES          下载免费PDF全文

Marcos P. Santos  Santiago D. Gutierrez‐Nibeyro  Alexia L. McKnight  Kuldeep Singh 《Veterinary radiology & ultrasound》2015,56(4):407-416

With the recent introduction of a 0.25T rotating MRI system, clinical evaluation of the equine stifle joint is now possible in the average equine athlete. A recent publication described common abnormalities of horses with stifle lameness detected with a low‐field MRI system; however, postmortem corroboration of the lesions detected was not possible. Therefore, our objective was to compare postmortem findings with low‐field MRI findings in equine cadaver stifle joints. Ten fresh cadaver stifle joints from horses without clinical signs of stifle disease were evaluated using low‐field MRI, gross dissection, and histopathology. In eight stifles, either the lateral or medial cranial meniscotibial ligament had an irregular shape, fiber separation, or moderate abnormal signal intensity (SI) on all sequences. In five stifles, the medial femoral condyle had articular cartilage fibrillation with or without an osteochondral defect over the weight bearing surface of the medial femoral condyle. All stifles had abnormal SI on all sequences within the patellar ligaments that corresponded with adipose tissue infiltrating between the collagen bundles. Other abnormalities identified included articular cartilage fibrillation of the tibial condyles in three stifles, and articular cartilage fibrillation with chondral defects in the patella in three stifles. All abnormalities detected with low‐field MRI were corroborated by gross dissection. Findings from the current study supported the use of low‐field MRI for detection of stifle joint lesions in horses and demonstrated that some stifle joint pathologies may be subclinical in horses.  相似文献