Laboratory diagnosis and disease occurrence in the current African swine fever eradication program in Spain, 1989–1991     

Steen Bech-Nielsen  Maria Louisa Arias  Jesus Panadero  Jose M. Escribano  Concepcion Gomez-Tejedor  Q. Perez Bonilla  J. M. Sanchez-Vizcaino 《Preventive veterinary medicine》1993,17(3-4):225-234

The purpose of this study was to determine the rate of decline and geographical distribution by municipality of clinical and subclinical African swine fever (ASF) in the affected areas of Spain. A second aim was to evaluate the performance of diagnostic tests in the Spanish ASF eradication program. Clinical outbreaks were confirmed using both the direct and indirect immunofluorescence test (and if both were negative, by the hemabsorption test). The serological status of swine was determined by an enzyme-linked immunosorbent assay (ELISA) and suspect serum samples were confirmed by the immunoblot assay.

The number of clinical outbreaks (herds) of ASF for 1989, 1990 and 1991 was 170, 347 and 207, respectively. The numbers of municipalities within each affected province experiencing acute outbreaks for the same time periods were 49, 69 and 48, respectively. Serologically diagnosed animals positive for ASF were 1.1% of animals tested in 1989, 0.5% in 1990 and 0.8% in 1991. The corresponding positive predictive values of the standard ELISA test used were 99.0, 97.9 and 98.8, respectively. Similarly, the number of municipalities within each affected province experiencing serologically positive subclinically infected animals was 269, 178 and 147 for each of the years 1989, 1990 and 1991, respectively.  相似文献   

The effect of aging of milk samples on the accuracy of infrared analysis of milk composition]     

O Hanus  V Gencurová  B Gabriel 《Veterinární medicína》1992,37(3):149-160

The effect of a decrease (and/or fermentation) in the lactose content during milk storage under different conditions was investigated on the accuracy of the results obtained on a Milko-Scan apparatus to contribute to the present knowledge of this problem. The results were in agreement with some results cited in the literature. These wavelengths are used for infrared spectrophotometry on the above apparatus: for fat 3.48 microns, for proteins 6.46 microns and for lactose 9.60 microns. Bulk milk samples used for the tests were untreated or treated with potassium dichromate, bronopol, sodium azide and Milkofix at the temperatures of storage in darkness 20 degrees C and 4 degrees C. The differences against the reference values (measured on the first day) were determined and evaluated in milk composition and characteristics as arising during milk storage. These differences were used in form of either cumulative means of differences (Figs. 1 to 5) or individual differences (Fig. 8). In the first part significant correlation coefficients (P less than 0.001) were calculated for the relationship between the variations of lactose content and the fat and protein contents: r = -0.59 and/or -0.73 (Figs. 6 and 7). This suggests that the decrease in the lactose content by 0.10% recorded by the infrared analysis and caused by lactose decomposition is accompanied by a "seeming" increase in the fat and protein content by about 0.04%. In the second part the correlation coefficients for the fat and protein contents r = -0.96 and -0.96 (P less than 0.001; Figs. 9 and 10; Tab. II) were calculated on the basis of an observation of the lactose decrease in an untreated milk sample (20 degrees C for 28 hours). These coefficients are somewhat different from the preceding ones; this is due to the lower homogeneity of the first set where the milk samples were treated in a different way, but the coefficients confirm the same conclusions. The values of the correlation coefficients for the dependence between the development of the acquired titratable acidity (SH) and the variations of fat (F), protein (P) and lactose (L) contents were as follows: r = 0.95; 0.95; -0.99 (P less than 0.001; Figs. 12, 13; Tab. II). Thus the above-mentioned "seeming" increase in the F and P contents can be explained to the extent of 92.2% from the decrease in the L content, which also causes the increase in titratable acidity to the extent of 98.0%.(ABSTRACT TRUNCATED AT 400 WORDS)  相似文献   

Some determinants of the spatio-temporal fire cycle in a mediterranean landscape (Corsica,France)     

Mouillot  Florent  Ratte  Jean-Pierre  Joffre  Richard  Moreno  Jose Manuel  Rambal  Serge 《Landscape Ecology》2003,18(7):665-674

Based on recent needs to accurately understand fire regimes and post-fire vegetation resilience at a supra-level for carbon cycle studies, this article focusses on the coupled history of fire and vegetation pattern for 40 years on a fire-prone area in central Corsica (France). This area has been submitted since the beginning of the 20^th century to land abandonment and the remaining land management has been largely controlled by frequent fires. Our objectives were to rebuild vegetation and fire maps in order to determine the factors which have driven the spatial and temporal distribution of fires on the area, what were the feed backs on the vegetation dynamics, and the long-term consequences of this inter-relationship. The results show a stable but high frequency of small fires, coupled with forest expansion over the study period. The results particularly illustrate the spatial distribution of fires according to topography and vegetation, leading to a strong contrast between areas never burnt and areas which have been burnt up to 7 times. Fires, when occuring, affect on average 9 to 12% of the S, SE and SW facing slopes (compared to only 2 to 5% for the N facing slopes), spread recurrently over ridge tops, affect all the vegetation types but reburn preferentially shrublands and grasslands. As these fire-proning parameters have also been shown to decrease the regeneration capacity of forests, this study highlights the needs in spatial studies (both in terms of fire spread and vegetation dynamic) to accurately apprehend vegetation dynamic and functionning in fire-prone areas.This revised version was published online in May 2005 with corrections to the Cover Date.  相似文献   

Use of two doses of cloprostenol in different intervals for estrus synchronization in hair sheep under tropical conditions     

de Carvalho Menezes de Almeida  Sheylla Foligno  Souza-Fabjan  Joanna Maria Gonçalves  Balaro  Mario Felipe Alvarez  Bragança  Gláucia Mota  Pinto  Pedro Henrique Nicolau  de Almeida  José Gabriel  Moura  Ana Beatriz Bossois  da Fonseca  Jeferson Ferreira  Brandão  Felipe Zandonadi 《Tropical animal health and production》2018,50(2):427-432

Tropical Animal Health and Production - This study evaluated the effect of two doses of prostaglandin at different intervals on reproductive parameters of crossbred ewes. In Experiment 1, 30 ewes...  相似文献   

Early resynchronization protocols for goats: Progestogens can be used prior to an early pregnancy diagnosis without affecting corpus luteum functionality     

Isabel Oliveira Cosentino  Mario Felipe Alvarez Balaro  Felipe Seabra Cardoso Leal  Lucas de Figueiredo Cardoso Barbosa  Fernanda Martins Gonçalves  Gabriel Feliciano Felizardo  Marina Monteiro Netto  Felipe Zandonadi Brandão 《Reproduction in domestic animals》2020,55(11):1655-1659

This study aimed to evaluate the exogenous progesterone (P4) effect on the luteal function from Day 16 to Day 21 of the oestrous cycle in inseminated goats with unknown pregnancy status. A total of 54 does passed through a short progestin-based synchronization protocol and, on Day 16 of the following oestrous cycle, 27 does received a new P4 device which was retained until Day 21. Blood samples were collected daily from all does during this period, as well as on Day 24. Pregnancy diagnoses were performed on Day 30. Serum P4 values from 26 animals (G_NPSP: Group of non-pregnant does with second sponge: n = 8; G_NPNSP: Group of non-pregnant does without second sponge: n = 6; G_PSP: Group of pregnant does with second sponge: n = 5; G_PNSP: Group of pregnant does without second sponge: n = 7) were determined by radioimmunoassay commercial kits. No P4 differences were found between groups (G_NPSP: 3.1 ± 2.8; 1.7 ± 1.8; 0.4 ± 1.0; and 0.0 ± 0.0 vs. G_NPNSP: 4.4 ± 1.8; 3.0 ± 2.2; 0.8 ± 0.8; and 0.0 ± 0.0 or G_PSP: 4.2 ± 1.0; 3.4 ± 0.6; 3.3 ± 1.6; 3.2 ± 0.9; 3.6 ± 1.2; 3.5 ± 1.3; 2.7 ± 1.3 vs. G_PNSP: 4.4 ± 1.6; 3.6 ± 1.5; 3.7 ± 1.5; 3.8 ± 1.4; 3.2 ± 1.2; 3.1 ± 1.2; 3.6 ± 1.1; D16, D17, D18, D19, D20, D21, D24, respectively) or for the interaction of group and time. In conclusion, a second progestogen device had no effect on luteolysis or early pregnancy in the following oestrous cycle.  相似文献   

Thermal biology and locomotor performance in Phymaturus calcogaster: are Patagonian lizards vulnerable to climate change?     

Rosa L. OBREGÓN  Jose A. SCOLARO  Nora R. IBARGÜENGOYTÍA  Marlin MEDINA 《Integrative zoology》2021,16(1):53-66

Behavioral and physiological traits of ectotherms are especially sensitive to fluctuations of environmental temperature. In particular, niche-specialist lizards are dependent on their physiological plasticity to adjust to changing environmental conditions. Lizards of the genus Phymaturus are viviparous, mainly herbivorous, and inhabit only rock promontories in the steppe environments of Patagonia and the Andes. Herein, we examine the vulnerability of the southernmost Phymaturus species to global warming: the endemic Phymaturus calcogaster, which lives in a mesic environment in eastern Patagonia. We studied body temperatures in the field (T_b), preferred body temperatures in a thermogradient (T_pref), the operative (T_e) and environmental temperatures, and the dependence of running performance on body temperature. P. calcogaster had a mean T_b (27.04°C) and a mean T_e (31.15°C) both lower than their preferred temperature (T_pref = 36.61°C) and the optimal temperature for running performance (T_o = 37.13°C). Lizard activity seems to be restraint during the early afternoon due high environmental temperatures. However, both, the high safety margin and warming tolerance suggest that the expected increase in environmental temperatures due to global warming (IPCC report in 2018) would not threaten, but indeed enhance locomotor performance in this population.  相似文献   

Simplified strong ion difference approach to acid–base balance in healthy foals          下载免费PDF全文

Judit Viu DVM  PhD  Lara Armengou DVM  PhD  DECEIM  José Ríos BSc  Anna Muñoz DVM  PhD  Eduard Jose‐Cunilleras DVM  PhD  DACVIM  DECEIM 《Journal of Veterinary Emergency and Critical Care》2016,26(4):549-558

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Morphological and Immunohistochemical Characterization of Canine Osteosarcoma Spheroid Cell Cultures          下载免费PDF全文

C. Gebhard  C. Gabriel  I. Walter 《Anatomia, histologia, embryologia》2016,45(3):219-230

Spheroid cell culture emerges as powerful in vitro tool for experimental tumour research. In this study, we established a scaffold‐free three‐dimensional spheroid system built from canine osteosarcoma (OS) cells (D17). Spheroids (7, 14 and 19 days of cultivation) and monolayer cultures (2 and 7 days of cultivation) were evaluated and compared on light and electron microscopy. Monolayer and spheroid cultures were tested for vimentin, cytokeratin, alkaline phosphatase, osteocalcin and collagen I by means of immunohistochemistry. The spheroid cell culture exhibited a distinct network of collagen I in particular after 19‐day cultivation, whereas in monolayer cultures, collagen I was arranged as a lamellar basal structure. Necrotic centres of large spheroids, as observed in 14‐ and 19‐day cultures, were characterized by significant amounts of osteocalcin. Proliferative activity as determined by Ki‐67 immunoreactivity showed an even distribution in two‐dimensional cultures. In spheroids, proliferation was predominating in the peripheral areas. Metastasis‐associated markers ezrin and S100A4 were shown to be continuously expressed in monolayer and spheroid cultures. We conclude that the scaffold‐free spheroid system from canine OS cells has the ability to mimic the architecture of the in vivo tumour, in particular cell–cell and cell–matrix interactions.  相似文献   

Quantitative motor unit action potential analysis of supraspinatus,infraspinatus, deltoideus and biceps femoris muscles in adult Royal Dutch sport horses          下载免费PDF全文

E. Jose‐Cunilleras  I. D. Wijnberg 《Equine veterinary journal》2016,48(2):234-239

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Neuroaxonal dystrophy in dogs: case report in 2 litters of Papillon puppies     

Diaz JV  Duque C  Geisel R 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(3):531-534

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