排序方式： 共有11条查询结果，搜索用时 33 毫秒
1.
Dynamics of cytokine gene expression in peripheral blood mononuclear cells of indigenous and exotic breeds of pigs in India 
下载免费PDF全文
下载免费PDF全文 2.
3.
Gaurav?KrishnaEmail author P.?Sairam?Reddy Pramod?W.?Ramteke Pogiri?Rambabu Kailas?Bhagawanrao?Tawar Parthasarathi?Bhattacharya 《Journal of Crop Science and Biotechnology》2011,14(3):197-204
Agrobacterium-mediated genetic transformation was performed using embryonic axes explants of pigeon pea. Both legume pod borer resistant
gene (cry1Ac) and plant selectable marker neomycine phosphor transferase (nptII) genes under the constitutive expression of the cauliflower mosaic virus 35S promoter (CaMV35S) assembled in pPZP211 binary
vector were used for the experiments. An optimum average of 44.61% successfully hardened dot blot Southern hybridization positive
plants were obtained on co-cultivation media supplemented with 200 μM acetosyringone without L-cysteine. The increased transformation
efficiency from a baseline of 11.53% without acetosyringone to 44.61% with acetosyringone was further declined with the addition
of different concentrations of L-cysteine to co-cultivation media. Transgenic shoots were selected on 50 and 75 mg L−1 kanamycin. Rooting efficiency was 100% on half-strength Murashige and Skoog medium with 20 g L−1 sucrose and 0.5 mg L−1 indole butyric acid in the absence of kanamycin. Furthermore, 100% seed setting was found among all the transgenic events.
The plants obtained were subjected to multi- and nochoice tests to determine the behavioral responses and mortality through
Helicoverpa armigera bioassays on the leaf and relate their relationship with the expression of cry1Ac protein which was found to be less in leaf as compared to the floral buds, anther, pod, and seed. 相似文献
4.
Jesu Arockiaraj Rajesh Palanisamy Prasanth Bhatt Venkatesh Kumaresan Annie J. Gnanam Mukesh Pasupuleti Marimuthu Kasi 《Fish physiology and biochemistry》2014,40(6):1937-1955
We have reported the molecular characterization including gene silencing, superoxide activity, superoxide anion production, gene expression and molecular characterization of a mitochondrial manganese superoxide dismutase (mMnSOD) from striped murrel Channa striatus (named as CsmMnSOD). The CsmMnSOD polypeptide contains 225 amino acids with a molecular weight of 25 kDa and a theoretical isoelectric point of 8.3. In the N-terminal region, CsmMnSOD carries a mitochondrial targeting sequence and a superoxide dismutases (SOD) Fe domain (28–109), and in C-terminal region, it carries another SOD Fe domain (114–220). The CsmMnSOD protein sequence shared significant similarity with its homolog of MnSOD from rock bream Oplegnathus fasciatus (96 %). The phylogenetic analysis showed that the CsmMnSOD fell in the clade of fish mMnSOD group. The monomeric structure of CsmMnSOD possesses 9 α-helices (52.4 %), 3 β-sheets (8.8 %) and 38.8 % random coils. The highest gene expression was noticed in liver, and its expression was inducted with fungal (Aphanomyces invadans) and bacterial (Aeromonas hydrophila) infections. The gene silencing results show that the fish that received dsRNA exhibited significant (P < 0.05) changes in expression when compared to their non-injected and fish physiological saline-injected controls. The SOD activity shows that the activity increases with the spread of infection and decreases once the molecule controls the pathogen. The capacity of superoxide anion production was determined by calculating the granular blood cell count during infection in murrel. It shows that the infection influenced the superoxide radical production which plays a major role in killing the pathogens. Overall, this study indicated the defense potentiality of CsmMnSOD; however, further research is necessary to explore its capability at protein level. 相似文献
5.
Cellulase, amylase, invertase, protease and phosphatase activities in pressmud (PM; filter cake) and PM vermicasts of fresh,
15- and 30-day-old casts of Lampito mauritii and Eudrilus eugeniae decreased considerably with cast age. Enhancement of the activities of these enzymes could be ascribed to the nutrient-rich
substrate, active microbial populations and optimal moisture conditions. Aged casts showed reduced enzyme activities because
of a decreased moisture content, lower nutrient concentrations and a decline in microbial activity. Earthworm-microbe symbiosis
seems to operate in a stepwise fashion, controlling groups of enzymes during the metabolism of PM to keep the flux of nutrients
in casts (soil) in balance in the biosphere.
Received: 20 November 1998 相似文献
6.
Carbon films with a diamondlike structure that are chemically bonded to surfaces have been deposited by means of low-energy C(+) ion beams. When mass-selected C(+) beams at energies in the range from 20 to 200 electron volts impinge on atomically clean surfaces, the first carbon monolayer grows as a carbide structure that is chemically bonded to the surface. As deposition continues, the structure evolves over the next several atomic layers into a diamondlike structure. These pure carbon films are strongly adhered to the surface through the carbide bonds, which also provide for an intimate interface. There are significant applications for such films, particularly as insulators and doped semiconductors. 相似文献
7.
Longevity of microbial and enzyme activity and their influence on NPK content in pressmud vermicasts
Total microbial population and their activity (CO2 evolution), macronutrients (N, P, K) and enzymes (dehydrogenase and phosphatase activities) in the uningested pressmud, fresh casts and 15- and 30-d-old pressmud vermicasts of two composting worms, Lampito mauritii and Eudrilus eugeniae, have been determined. Enhancement of microbial population and activity, NPK content and enzyme activities in the fresh casts are due to enhanced mineralization of nutrients, high substrate concentrations and high moisture level. As the vermicast become aged, there is a reduction in the moisture level leading to reduction in microbial population and activity. Consequently reduced microbial activity leads to reduced enzyme activity and NPK contents. Dryness of aged vermicasts implies the loss of N through volatilization, leaching and denitrification. Reduction in P is due to lesser conversion of organic to inorganic P due to reduced microbial activity. Loss of K input is due to leaching.
Résumé
La population microbienne totale et son activité (dégagement de CO2), les nutiments (N, P, K) et les activités enzymatiques (déshydrogénase et phosphatase) dans des résidus de canne à sucre (bagasse), ainsi que dans des turricules frais et des turricules âgés de 15 et 30 j, formés à partir de ce matériau organique par deux vers de terre composteurs, Lampito mauritii et Eudrilus eugeniae, ont été étudiés. L'augmentation de la population bactérienne et son activité, des teneurs en N, P, K et des activités enzymatiques dans les turricules frais sont liés à un accroissement de la minéralisation des nutriments, à la forte concentration en substrat organique et à la forte teneur en eau. Lorsque les turricules vieillissent, la réduction de cette teneur en eau conduit à une diminution de la population bactérienne et de son activité. Ceci conduit également, par conséquent, à une diminution des activités enzymatiques et des teneurs en N, P, K. La dessiccation des turricules entraîne une perte d'azote par volatilisation, lessivage et dénitrification. La réduction des teneurs en phosphore est due à une moindre conversion du P organique en P inorganique par les microorganismes. La perte de potassium se fait par lessivage. 相似文献8.
建立了一种考虑催化再生的微粒捕集器碳烟沉积量估计模型。通过试验数据辨识出NO_2与碳烟的反应以及热催化再生反应参数和载体捕集特性参数。在不同工况下,碳烟沉积量估计模型的计算值与实际碳载量称量值相比,误差可以控制在10%以内。经过计算,随着排气温度的不断提升,碳烟再生量权重不断提升,催化再生有效地降低了颗粒物沉积量,延长了主动再生周期。通过深床层离散分层和饼层累积碳载量计算,碳烟加载量主要分布在饼层,深床层占比较少,且深床层碳烟量主要集中在离散层的第1层中。经过验证,引入NO_2辅助再生及热催化再生反应的模型有效地估计了碳烟加载量并对碳载量分布特性给出了有效的分析,为微粒捕集器内碳烟颗粒物主动再生时机的判断提供了参考。 相似文献
9.
K.S. Moorthy B.Kasi Reddy K.S. Swami C.S. Chetty 《Pesticide biochemistry and physiology》1985,24(1):40-44
Changes in glucose metabolism were studied in hepatopancreas and gill of freshwater mussel, Lamellidens marginalis, exposed to a sublethal concentration (8 ppm) of methyl parathion. A slight decrease in glycogen and pyruvate and an increase in lactate levels were observed. An increase in phosphorylase and aldolase suggested increased formation of trioses during methyl parathion toxicity. The decrease in lactate dehydrogenase activity and increase in lactate content indicated reduced mobilization of pyruvate into the citric acid cycle. Glucose-6-phosphate dehydrogenase activity was increased, suggesting enhanced oxidation of glucose by the HMP shunt pathway. Citric acid cycle enzymes such as isocitrate, succinate, and malate dehydrogenases were found to be decreased, suggesting abnormality in mitochondrial oxidative metabolism as a consequence of methyl parathion toxicity. The decreased cytochrome c oxidase and Mg2+-ATPase, apart from citric acid cycle enzymes, indicated impaired energy synthesis as a result of reduced aerobic oxidation of glycose. The increase in acid and alkaline phosphatase activities suggested enhanced breakdown of phosphate to release energy in view of inhibition of the ATPase system during methyl parathion stress. The changes were more pronounced in hepatopancreas as compared to gill of mussel exposed to methyl parathion. 相似文献
10.
This is a study of trace metal competition in the complexation of Pb(II) by well-characterized humic substances, namely Suwannee River Fulvic Acid (SRFA) in model solutions. It was found that Cu(II) seems to compete with Pb(II) for strong binding sites of SRFA when present at the same concentration as Pb(II). However, Cd(II) and Zn(II) did not seem to compete with Pb(II) for strong binding sites of SRFA. These two metals did compete with Pb(II) for the weaker binding sites of SRFA. Heterogeneity of SRFA was found to play a crucial role in metal–SRFA interactions. The environmental significance of this research for freshwater is that even at relatively low Pb(II) loadings, the metals associated with lead in minerals, e.g. Cu(II), may successfully compete with Pb(II) for the same binding sites of the naturally occurring organic complexants, with the result that some of the Pb(II) may exist as free Pb2+ ions, which has been reported to be one of the toxic forms of Pb in aquatic environment. 相似文献