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1.
Four experiments with 270, 44, 432 and 66800 Leghorn hens were carried out to investigate the influence of various Mn additions to diets differed in mineral or Ca contents on egg shell quality. The addition of 300 mg Mn/kg diet improved significantly egg shell breaking strength by 4 N over one year. The supply of 50-500 mg Mn/kg diet for 10-24 weeks of the second half of laying year did not influence the egg shell quality. Addition of mineral mixture or Ca grit to layer rations with adequate or higher Mn levels did not influence egg shell strength. High mineral content in a low manganese diet increased number of cracks by 3%. Strength, weight and ash content of tibia were significantly reduced by feeding a low mineral level. Addition of 50-150 mg Mn per kg low mineral diet normalized partially tibia stability in young hens. It was concluded that supplied dietary Manganese influences calcification positively only in young hens. High levels of Ca did not influence the effects of Mn. 50 mg Mn per kg layers mixture have been considered as an essential supply.  相似文献   
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Two adult female rock hyraxes (Procavia capensis) at the Dallas Zoo were confirmed with spontaneous diabetes mellitus from 1997-2000, whereas a third animal with a similar clinical presentation never became hyperglycemic. The pancreas in all three animals showed pancreatic islet fibrosis (PIF). Retrospective examination of medical records for rock hyraxes acquired by this collection or born into it from 1991-2002 identified eight more animals affected with PIE All affected animals, including three males and eight females, were 1-7 yr of age and presented either with vague clinical signs of soft feces and rough hair coat or were acutely moribund or dead. Clinical pathology data was available for seven of the animals before onset of overt clinical signs and revealed inappropriate hyperglycemia in six, as well as elevated serum concentrations of creatine phosphokinase, amylase, and lipase in all seven animals. Pedigree evaluation did not support a familial pattern for PIE Review of the histopathology findings from nine other zoologic collections with rock hyrax deaths during the study period identified six institutions with 12 additional cases genetically unrelated to the incident collection. Histopathology and viral serology did not support an infectious cause. Analysis of serum anti-islet and anti-insulin antibodies did not suggest autoimmune disease, and none of the animals had known exposure to toxic substances. Limited nutritional analyses did not support a nutritional basis for the condition, and the cause for PIF remains unknown.  相似文献   
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Bacterial blood culture results of 292 privately owned cats presented to the Clinic for Small Animal Medicine, Ludwig Maximilian University Munich with signs of sepsis were evaluated retrospectively. Of the blood cultures, 23% were positive. In 88%, a single bacterial species was isolated. Of all bacterial isolates, 45% were Gram-positive, 43% were Gram-negative, and 12% were obligate anaerobes. The most frequently isolated bacteria were Enterobacteriaceae, obligate anaerobic species, Staphylococcus species and Streptococcus species. Of the cats with positive blood cultures, 32% were pretreated with antibiotics. Of all bacterial isolates, 77% were susceptible to enrofloxacin, 69% to chloramphenicol, 67% to gentamicin, and 64% to amoxycillin clavulanic acid. Only enrofloxacin reached an in vitro efficacy of more than 70% against Gram-positive and more than 74% against Gram-negative bacteria.  相似文献   
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精准农业的目的就是要按照作物生长需要有针对性地进行栽培管理。该文从耕种方式、播种方式、施肥方式以及农田作业轨迹方面出发,介绍了现行一些精准农业发展情况,同时指出了所面临的问题及发展方向。   相似文献   
7.
Evaluation of ultrasonic sensor for variable-rate spray applications   总被引:2,自引:0,他引:2  
Automatic variable-rate sprayers require accurate measurement of canopy size. An estimate of canopy size is made by measuring the distance to the canopy at several elevations above the ground; an ultrasonic sensor was used to determine canopy distance in this study. It is sometimes necessary to conduct spray operations during harsh operating conditions. In this study ultrasonic sensors were subjected to simulated environmental and operating conditions to determine their durability and accuracy. Conditions tested included exposure to extended cold, outdoor temperatures, cross winds, temperature change, dust clouds, travel speeds and spray cloud effects. The root mean square (RMS) error in a series of measurements of the distance to a simulated plant canopy was used to test for significant difference among treatments. After exposure to outdoor cold conditions for 4 months, the RMS error in distance measured by the ultrasonic sensor increased from 3.31 to 3.55 cm, which was not statistically significant. Neither the presence of dust cloud nor the changes in cross-wind speeds over a range from 1.5-7.5 m/s had significant effects on the mean RMS errors. Varying sensor travel speed from 0.8 to 3.0 m/s had no significant influence on sensor detection distances. Increasing ambient temperature from 16.7 to 41.6 °C reduced the detection distance by 5.0 cm. The physical location of the spray nozzle with respect to the ultrasonic sensor had a significant effect on mean RMS errors. The mean RMS errors of sensor distance measurements ranged from 2.3 to 83.0 cm. The RMS errors could be reduced to acceptable values by proper controlling the sensor/spray nozzles spacing on a sprayer. In addition, multiple-synchronized sensors were tested for their measurement stability and accuracy (due to possible cross-talk errors) when mounted on a prototype sprayer. It was found that isolating the pathway of the ultrasonic wave of each sensor reduced detecting interference between sensors during multiple sensor operation. Test methods presented herein may be useful in the design of standardized testing protocols for field use distance sensors.  相似文献   
8.
Krause MS  Madden LV  Hoitink HA 《Phytopathology》2001,91(11):1116-1123
ABSTRACT Potting mixes prepared with dark, highly decomposed Sphagnum peat, with light, less decomposed Sphagnum peat, or with composted pine bark, all three of which were colonized by indigenous microorganisms, failed to consistently suppress Rhizoctonia damping-off of radish or Rhizoctonia crown and root rot of poinsettia. Inoculation of these mixes with Chryseobacterium gleum (C(299)R(2)) and Trichoderma hamatum 382 (T(382)) significantly reduced the severity of both diseases in the composted pine bark mix in which both biocontrol agents maintained high populations over 90 days. These microorganisms were less effective against damping-off in the light and dark peat mixes, respectively, in which populations of C(299)R(2) declined. In contrast, crown and root rot, a disease that is severe late in the crop, was suppressed in all three types of mixes. High populations of T(382) in all three mixes late during the cropping cycle may have contributed to control of this disease.  相似文献   
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Cell cultures infected with BHV-1/F(syn), a recombinant bovine herpesvirus 1 (BHV-1) which expresses a synthetic open reading frame encoding the fusion (F) protein of the bovine respiratory syncytial virus (BRSV), showed a cytopathic effect (CPE) indistinguishable from that induced by wildtype BHV-1 although transient transfection experiments demonstrated that expression of the F protein leads to formation of large syncytia. Since it has been shown that glycoprotein M (gM) of pseudorabies virus inhibits BRSV F-induced syncytium formation in transient plasmid transfection experiments [Pseudorbies virus glycoprotein M inhibits membrane fusion. J. Virol. 74 (2000) 6760], the gM ORF of wtBHV-1 and BHV-1/F(syn) was interrupted. Infection of cell cultures with the resulting gM(-) mutant of BHV-1/F(syn) led to formation of syncytia, whereas the CPE in gM(-)BHV-1 infected cells was comparable to the CPE in wtBHV-1 infected cultures. Our results demonstrate that gM is not essential for BHV-1 replication in cell culture and that gM is involved in inhibition of the cell fusion activity of the BHV-1 expressed BRSV F protein.  相似文献   
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