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Teruo NONOMURA Yoshinori MATSUDA Motoaki TSUDA Kazuto URANAKA Hideyoshi TOYODA 《Journal of General Plant Pathology》2001,67(3):224-227
Commercially available tomato cultivars were hydroponically cultured for inoculation, with Ralstonia solanacearum (K-101), which causes bacterial wilt, by pouring an inoculum suspension into the nutrient solution. Cultivar susceptibility
to the bacteria was evaluated, based on the highest percentage of wilting. Because the length of time for wilt appearance
varied among cultivars, some cultivars appeared to be suppressive to the translocation and/or multiplication of the invading
pathogen. Thus, this hydroponic inoculation system is effective for examining levels of susceptibility in tomato cultivars
to bacterial wilt.
Received 13 December 2000/ Accepted in revised form 27 March 2001 相似文献
4.
Enose Y Miyake A Ido E Hayami M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(2):283-286
Human immunodeficiency virus (HIV) infects lymphocytes and macrophages via CD4 and chemokine receptors. In this study, the infectivity of a chimeric simian and human immunodeficiency virus (SHIV) having a CCR5-specific HIV-1 envelope gene was examined. A SHIV strain termed SHIV-JRFL could enter cells via CD4 with a chemokine receptor CCR5, not CXCR4, and the viral replication was suppressed by recombinant human RANTES, one of beta-chemokines. The intravenous inoculation of SHIV-JRFL into two rhesus macaques resulted in a systemic infection, though it was rather weak. During the early infection, the production of RANTES from Con A-stimulated PBMCs of the infected monkeys increased. These results suggested that beta-chemokine has the potential to limit the infectivity of an R5-type SHIV. 相似文献
5.
Hoque S Derar RI Senba H Osawa T Kano K Taya K Miyake Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):713-717
To clarify the morphological and immunohistochemical characteristics in mares with granulosa theca cell tumor (GTCT), the localization of inhibin subunits (alpha, betaA, betaB) and aromatase in the granulosa cell layers and theca layers in the ovarian follicles were determined by immunohistochemical staining. The follicles were obtained from the ovaries of 6 mares with GTCT and 4 normal mares as controls. Immunohistochemically, inhibin alpha-subunit was localized in the granulosa cells of all follicles showing different sizes in all GTCT cases and betaA- subunit was localized in two GTCT cases in all sized follicles. But inhibin betaB- subunit and aromatase were not localized in GTCT cases. On the other hand, inhibin alpha-, betaA-, and betaB-subunits and aromatase were localized in the large and medium sized follicles, but inhibin betaA- and betaB-subunits and aromatase were not stained in the small sized follicles in normal cases. These findings suggest that some mares with GTCT can secrete dimeric inhibin (inhibin A), but all GTCT cases cannot secrete inhibin B. By the results of aromatase staining it is clear that testosterone is not converted into estradiol due to the lack of aromatase in the GTCT follicles. 相似文献
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Junji Nishikawa Takao Kobayashi Kazuto Shirata Takashi Chibana Keiko T. Natsuaki 《Journal of General Plant Pathology》2006,72(5):305-313
We isolated 629 fungi from 1296 berry seeds of solanaceous plants, including tomato (Lycopersicon esculentum), eggplant (Solanum melongena), bell pepper (Capsicum annuum), and red pepper (Capsicum annuum var. annuum) preserved for long and short terms. The isolates were classified into 22 genera excluding unidentified fungi, and the fungal
floras were divided into two types: the tomato–eggplant and pepper groups. The results of cluster analysis with unweighted
pair-group method with arithmetic average also supported these groups. Most tomato seeds infested with Geotrichum candidum germinated and grew the same as uninfested seeds. Cladosporium sphaerospermum and Arthrinium sp. isolated from eggplant seeds strongly suppressed germination, and Penicillium variabile suppressed seminal root elongation on eggplant. Alternaria alternata, Botrytis cinerea, and Myrothecium verrucaria detected from red pepper or bell pepper seeds were pathogenic to the fruits and the seedlings after artificial inoculation. 相似文献
8.
1. Changes in plasma alpha1-acid glycoprotein (AGP) concentration and immune responses following Escherichia coli lipopolysaccharide (LPS) injection were studied in broiler chickens. 2. Higher plasma AGP concentrations were observed from 12 to 48 h after a single injection of LPS. 3. The highest concentration of plasma AGP was observed on day 2 followed by a gradual decrease in chicks injected with 150 mug/kg body weight of LPS every day for 13 d. 4. Plasma AGP concentration in chicks injected daily with LPS at 900 mug/kg body weight for 13 d increased on day 2, and decreased on day 4 to the concentration found before the injection. The concentration increased again on day 10. 5. Changes in plasma interleukin-1 (IL-1) like activity were similar to those in plasma AGP concentration when LPS was injected daily at 900 mug/kg body weight for 3 d. 6. Responses of blood mononuclear cell (MNC) proliferation to mitogen or concanavalin A, (Con A), and pokeweed mitogen (PWM) were positively correlated with changes in plasma AGP concentration. 7. The results suggest that plasma AGP concentration could be used as a positive indicator of changes in blood MNC proliferation to a mitogen and in plasma IL-1 like activity. 相似文献
9.
Akio?InoueEmail author Marie?Miyake Tomohiro?Nishizono 《Journal of Forest Research》2004,9(4):319-324
An allometric model that explains the mechanism of the difference in the slope of the Reineke equation (A) among species was proposed based on the allometric relationships of mean tree height (H) to quadratic mean diameter D (H ∝ D
θ
) and stand density N (H ∝ N
δ
), i.e., A = θ/δ. The proposed model was fitted to Japanese cypress (Chamaecyparis obtusa Endl.) and red pine (Pinus densiflora) stands. The allometric exponents θ and δ were, respectively, 0.8995 and −0.5000 for cypress and 0.8612 and −0.6619 for pine. The difference between cypress and pine
was significant for δ but not for θ. Inserting the exponents into the model resulted in predicted slopes of −1.7991 for cypress and −1.3011 for pine. The difference
in the slope of the Reineke equation between the two species was produced by characteristics related to the tree crown, rather
than characteristics related to stem slenderness. The proposed model enables us to estimate the slope of the Reineke equation
from commonly measured stand attributes, such as mean tree height and quadratic mean diameter. Therefore, the proposed model
is expected to be practical and convenient for estimating the slope of the Reineke equation and for explaining the mechanism
of its variation among species. The model should be also accepted as a generalized model of the stand density versus quadratic
mean diameter relationship, whereas the original Reineke equation should be seen as a specific case of this model. 相似文献
10.
Jun TAMURA Tomohito ISHIZUKA Sho FUKUI Norihiko OYAMA Kodai KAWASE Kenjiro MIYOSHI Tadashi SANO Kirby PASLOSKE Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):289-296
The pharmacological effects of the anesthetic alfaxalone were evaluated after
intramuscular (IM) administration to 6 healthy beagle dogs. The dogs received three IM
doses each of alfaxalone at increasing dose rates of 5 mg/kg (IM5), 7.5 mg/kg (IM7.5) and
10 mg/kg (IM10) every other day. Anesthetic effect was subjectively
evaluated by using an ordinal scoring system to determine the degree of neuro-depression
and the quality of anesthetic induction and recovery from anesthesia. Cardiorespiratory
variables were measured using noninvasive methods. Alfaxalone administered IM produced
dose-dependent neuro-depression and lateral recumbency (i.e., 36 ± 28 min, 87 ± 26 min and
115 ± 29 min after the IM5, IM7.5 and IM10 treatments, respectively). The endotracheal
tube was tolerated in all dogs for 46 ± 20 and 58 ± 21 min after the IM7.5 and IM10
treatments, respectively. It was not possible to place endotracheal tubes in 5 of the
6 dogs after the IM5 treatment. Most cardiorespiratory variables remained
within clinically acceptable ranges, but hypoxemia was observed by pulse oximetry for 5 to
10 min in 2 dogs receiving the IM10 treatment. Dose-dependent decreases in rectal
temperature, respiratory rate and arterial blood pressure also occurred. The quality of
recovery was considered satisfactory in all dogs receiving each treatment; all the dog
exhibited transient muscular tremors and staggering gait. In conclusion, IM alfaxalone
produced a dose-dependent anesthetic effect with relatively mild cardiorespiratory
depression in dogs. However, hypoxemia may occur at higher IM doses of alfaxalone. 相似文献