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P. P. Strel’chenko O. I. Romanova A. V. Konarev K. Okuno 《Russian Agricultural Sciences》2010,36(3):168-171
STS and InDel markers developed on the basis of DNA sequences of certain rice genes are used for a comparative study of grain
sorghum accessions. The main sorghum genetic diversity centers are revealed, two in Africa and a third in regions of Central,
East, and South Asia. 相似文献
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AV Pereira SA Pereira IDF Gremião MP Campos AMR Ferreira 《Australian veterinary journal》2012,90(11):448-450
This study compared the sensitivity of acetate tape impression and skin squeezing with that of deep skin scraping for the diagnosis of demodicosis in dogs. Demodex canis was detected in 100% of acetate tape impressions obtained after skin squeezing and in 90% of deep skin scrapings. There was a significant difference (P < 0.001) between the techniques in the total number of mites detected. Acetate tape impression with skin squeezing was found to be more sensitive than deep skin scraping and is an alternative diagnostic method for canine demodicosis. 相似文献
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A. V. Konarev I. O. Vvedenskaya E. A. Nasonova I. N. Perchuk 《Genetic Resources and Crop Evolution》1995,42(3):197-209
Summary Electrophoresis of single seed prolamines was used for the analysis ofLolium perenne L.,Festuca pratensis Huds., andDactylis glomerata L. populations. Identification and registration of populations was carried out according to the frequencies of occurrence
of genotypes with corresponding types of prolamine banding patterns. The publication sums up the problems of applied use of
molecular markers for identification and registration of world genetic resources of forage grasses, analysis of the dynamics
of population composition and other problems of plant growing, genetics, breeding and seed control. The approaches mentioned
in the article are promising for their use in genetic banks as well as at the institutions which store collections of genetic
resources of given crops and at universities and breeding stations. 相似文献
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Objective To determine the usefulness of a snake venom detection kit (SVDK) in the management of envenomed cats.
Design A clinical study.
Twenty-two cats were investigated.
Procedure Cats injected subcutaneously with approximately 0.25 or 1.0 lethal dose (LD) of tiger snake venom or 1 or 4 LD of brown snake venom were observed for clinical symptoms of envenomation at intervals over the ensuring 24 to 48 hours(h). Blood and urine samples were taken at regular intervals and assayed in a quantitative laboratory assay for snake venoms. Selected samples were assayed in parallel in a rapid, semi-quantitative SVDK.
Results The studies showed that it was important to estimate the elapsed time from envenomation to presentation. If this time was less than 8 h, blood was the most appropriate sample and a negative result should exclude serious envenomation. If the elapsed time exceeded 8 h, it was essential that urine be sampled. Venom levels in urine were high at 8 h and approached the level of test sensitivity over 24 to 48 h; however by this time clinical signs were obvious in endangered cats.
Careful use of the SVDK is a valuable aid in the management of a potentially envenomed cat. 相似文献
Design A clinical study.
Animals
Twenty-two cats were investigated.
Procedure Cats injected subcutaneously with approximately 0.25 or 1.0 lethal dose (LD) of tiger snake venom or 1 or 4 LD of brown snake venom were observed for clinical symptoms of envenomation at intervals over the ensuring 24 to 48 hours(h). Blood and urine samples were taken at regular intervals and assayed in a quantitative laboratory assay for snake venoms. Selected samples were assayed in parallel in a rapid, semi-quantitative SVDK.
Results The studies showed that it was important to estimate the elapsed time from envenomation to presentation. If this time was less than 8 h, blood was the most appropriate sample and a negative result should exclude serious envenomation. If the elapsed time exceeded 8 h, it was essential that urine be sampled. Venom levels in urine were high at 8 h and approached the level of test sensitivity over 24 to 48 h; however by this time clinical signs were obvious in endangered cats.
Conclusions
Careful use of the SVDK is a valuable aid in the management of a potentially envenomed cat. 相似文献
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Alekseij?Konarev Nataliya?Gubareva Dimitri?Kornuchin Andreas?B?rnerEmail author 《Genetic Resources and Crop Evolution》2005,52(5):519-523
A standard electrophoretic method for wheat cultivar identification was used on single seeds to analyse the genetic integrity
of 11 wheat (Triticum aestivum L.) accessions after up to 24 seed reproductions in the Gatersleben genebank. It was clearly demonstrated that the gliadin
pattern of single seeds can be used to analyse the genotype composition of wheat accessions. Stability of electrophoretic
banding patterns was detected in eight accessions. Very week genetic drift was observed in three accessions. Our investigations
confirm experiences of the successful utilisation of protein markers for cultivar verification and genetic integrity testing
and demonstrate the high standard of wheat accessions maintenance in the Gatersleben genebank. 相似文献
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